• Signaling pathway of IL-15 begins with binding to IL-15Rα receptor, with subsequent presentation to surrounding cells bearing IL-15Rβγc complex on their cell surface. (wikipedia.org)
  • IL-15 is an inflammatory cytokine with structural similarity to Interleukin-2 (IL-2). (wikipedia.org)
  • Although IL-15 mRNA can be found in many cells and tissues including mast cells, cancer cells or fibroblasts, this cytokine is produced as a mature protein mainly by dendritic cells, monocytes and macrophages. (wikipedia.org)
  • Expression of IL-15 can be stimulated by cytokine such as GM-CSF, double-strand mRNA, unmethylated CpG oligonucleotides, lipopolysaccharide (LPS) through Toll-like receptors (TLR), interferon gamma (IFN-γ) or after infection of monocytes herpes virus, Mycobacterium tuberculosis and Candida albicans (Figure 2). (wikipedia.org)
  • Interleukin (IL)-15, a major cytokine involved in cross-talk between accessory cells (dendritic cells and macrophages) and NK cells, is produced by epithelial renal cells. (nih.gov)
  • Silencing of IL-15Ralpha by specific small interfering RNA in renal cell carcinoma had no effect on MbIL-15 production, indicating that the cytokine is not cross-presented by IL-15Ralpha in renal cell carcinoma cells but anchored to the membrane. (nih.gov)
  • Interleukin-23 ( IL-23 ) is a heterodimeric cytokine composed of an IL12B ( IL-12p40 ) subunit (that is shared with IL12 ) and the IL23A ( IL-23p19 ) subunit. (wikidoc.org)
  • Interleukin (IL)-15 belongs to the common gamma-dependent cytokine family, along with IL-2, IL-4, IL-7, IL-9, and IL-21. (hal.science)
  • Interleukin-21 (IL-21) is a type I cytokine that modulates functions of T, B, natural killer (NK), and myeloid cells. (nih.gov)
  • IL-15 is a regulatory cytokine that is produced by dendritic cells, epithelial cells, human stromal cell line (IMTLH), fibroblasts, and monocytes (2). (biolegend.com)
  • Interleukin-23 receptor is a type I cytokine receptor . (wikidoc.org)
  • IL-15 is an immunomodulating cytokine that stimulates the proliferation of T lymphocytes and shares many biological properties with IL-2. (prosci-inc.com)
  • Together with Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-7 (IL-7), Interleukin-9 (IL-9), granulocyte colony-stimulating factor (G-CSF), and granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-15 belongs to the four α-helix bundle family of cytokines. (wikipedia.org)
  • In this study, we addressed feeder cell-free expansion methods using common γ-chain cytokines, especially IL-15 and IL-21. (frontiersin.org)
  • The group of common γ-chain receptor cytokines encompassing interleukin (IL)-2, IL-4, IL-9, IL-15, and IL-21 has been studied intensively over the recent years. (frontiersin.org)
  • Levels of IL12RB1 mRNA are dynamically regulated by various cytokines, including interferon-γ (IFN-γ) and IL-15. (elsevierpure.com)
  • In addition, IL-15 promotes the development of dendritic cells (5), activates human neutrophils (6, 7) and induces the production of proinflammatory cytokines from macrophages (8). (biolegend.com)
  • Although the intestinal mucosa of CD patients contains increased amounts of Th1 associated cytokines, such as IFN-α, interleukin (IL)-18, and IL-15, the contribution of each of these cytokines in the ongoing Th1 response remains to be determined. (bmj.com)
  • Interleukin-12 (IL-12) and interleukin-23 (IL-23), which belong to the IL-12 family of cytokines, have a key role in intestinal homeostasis and inflammation and are implicated in the pathogenesis of inflammatory bowel disease. (nature.com)
  • In the present study, we investigated the effect of LPS on the releasing function of afferent terminals as measured by calcitonin gene-related peptide (CGRP) release in ex vivo perfused rat trachea, and examined the possible role of the cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) as intermediaries in this effect. (jneurosci.org)
  • Cytokines such as interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) can be generated by inflammatory and nonimmune cells in response to inflammation, tissue injury, microbial invasion, and immunological reactions ( Dinarello, 1994 ). (jneurosci.org)
  • Interleukin-15 (IL-15) is a protein that in humans is encoded by the IL15 gene. (wikipedia.org)
  • Interleukin-15 (IL15) and Bcl-2 associated × protein (Bax) levels were increased in the LTA compared with the RTA (p=0.006) and (p=0.005), respectively. (cdc.gov)
  • Development and characterization of new monoclonal antibodies against porcine Interleukin-17A and Interferon-gamma. (usda.gov)
  • Interferon γ (IFN-γ) and interleukin (IL)-4 RNA expression was examined in T lamina propria lymphocytes by quantitative reverse transcription-polymerase chain reaction. (bmj.com)
  • Seder, R. A., Gazzinelli, R., Sher, A. & Paul, W. E. Interleukin 12 acts directly on CD4+ T cells to enhance priming for interferon gamma production and diminishes interleukin 4 inhibition of such priming. (nature.com)
  • Percent body fat, plasma inflammatory markers associated with obesity (interferon (IFN)-γ, interleukin (IL)-10, IL-12 p70, IL-1β, IL-6 and KC/GRO), plasma Cur metabolites and liver telomere length were measured. (springer.com)
  • Interleukin-15 Receptor alpha Subunit" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (childrensmercy.org)
  • This graph shows the total number of publications written about "Interleukin-15 Receptor alpha Subunit" by people in this website by year, and whether "Interleukin-15 Receptor alpha Subunit" was a major or minor topic of these publications. (childrensmercy.org)
  • Below are the most recent publications written about "Interleukin-15 Receptor alpha Subunit" by people in Profiles. (childrensmercy.org)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Interleukin 10 (IL10) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. (orbitalbiosciences.com)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Cattle Interleukin 10 (IL10) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. (orbitalbiosciences.com)
  • Anti-Interleukin-15 (IL-15) Antibody, clone 1H3 detects level of Interleukin-15 (IL-15) and has been published and validated for use in western blotting. (fishersci.com)
  • Giron-Michel where it inhibits responses in cells attributable Emodin to human IL-15 (Eisenman cell systems (Bouchaud and activities for its potential use as a therapeutic antibody. (immune-source.com)
  • Methods Isolation of antibody DISC0280 Phage display technology was used to isolate a panel of novel human monoclonal single chain antibody fragments (scFv) specific for hIL-15 by performing selections to enrich for scFv that bind to biotinylated hIL-15 (Vaughan biological activity assays such as hIL-15 dependent survival of the mouse T cell line CTLL-2. (immune-source.com)
  • This antibody fragment was then optimized using phage display (Thompson data was performed using anova to analyse the entire data set then using the paired a mouse model was set up which measured the increase in NK1.1+ and CD3+ cells as a result of once daily dosing of hIL-15 over 3 days. (immune-source.com)
  • No. 515104) as the detecting antibody and recombinant human IL-15 (Cat. (biolegend.com)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Cattle Interleukin 17 (IL17) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. (orbitalbiosciences.com)
  • There is also an anti-IL-15 [interleukin-15] antibody study that's ongoing. (consultantlive.com)
  • This anti-IL-15 antibody, which is a subcutaneous injection, is an exciting study, and hopefully we'll see the results of that soon. (consultantlive.com)
  • Description: A sandwich ELISA kit for detection of Interleukin 18 from Guinea pig in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (ibiomagazine.org)
  • Description: A sandwich ELISA kit for detection of Interleukin 17 from Cattle in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (orbitalbiosciences.com)
  • Description: A sandwich ELISA kit for detection of Interleukin 22 from Cattle in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (orbitalbiosciences.com)
  • Description: Quantitativesandwich ELISA kit for measuring Chicken Interleukin 6, IL-6 in samples from serum, plasma, tissue homogenates. (elisastrip.com)
  • An unimportant IgG1 control does not have any influence on the response to IL-15. (immune-source.com)
  • IL-15 fused to the soluble domain of the IL-15R alpha chain. (fishersci.com)
  • and when administered together either as a complex or as a fusion protein of IL-15 with the extracellular 'sushi' domain of the IL-15R? (immune-source.com)
  • IL-15 was initially characterized as a soluble molecule. (wikipedia.org)
  • Masking of MbIL-15 with soluble IL-15Ralpha molecules restored NK cell apoptosis. (nih.gov)
  • This increase in cells caused by DISC0280/IL-15 co-administration was greater than that observed when IL-15 was administered complexed with soluble C3orf13 IL-15R? (immune-source.com)
  • binding site on IL-15 allows trans-presentation of IL-15 by DISC0280 similar to the trans-presentation by soluble IL-15R? (immune-source.com)
  • We demonstrate that DISC0280 inhibits Emodin the activity of soluble hIL-15 and prevents binding of hIL-15 to sIL-15R? (immune-source.com)
  • Interleukin-15 (IL-15) was discovered in supernatant from simian kidney epithelial cell line CV-1/EBNA, as a soluble factor capable of supporting proliferation of the IL-2-dependent cell line, CTLL-2 (1). (biolegend.com)
  • IL-15 is normally not secreted in soluble form but is held on the cell surface bound to a unique receptor, IL-15Rα, especially on dendritic cells. (biolegend.com)
  • The human IL-15 gene comprises nine exons (1-8 and 4A) and eight introns, four of which (exons 5 through 8) code for the mature protein (Figure 1). (wikipedia.org)
  • This discrepancy between the wide appearance of IL-15 mRNA and limited production of protein might be explained by the presence of the twelve in humans and five in mice upstream initiating codons, which can repress translation of IL-15 mRNA. (wikipedia.org)
  • Later it was shown that IL-15 also exists as a membrane-bound form which represents the major form of IL-15 protein. (wikipedia.org)
  • Consistently, IL-15, as a histone deacetylase inhibitor, synergistically enhanced IL12RB1 gene expression and promoter activation by IFN-γ through increased protein binding to ETS/PU.1 and IRE/ISRE sites. (elsevierpure.com)
  • To obtain a linear standard curve, serial dilutions of IL-15 recombinant protein ranging from 500 to 7.8 pg/ml are recommended for each ELISA plate. (biolegend.com)
  • Numerous reports suggest that interleukin-6 (IL-6) promotes survival and proliferation of tumor cells through the phosphorylation of a cell-signaling protein, signal-transducer-and-activator-of-transcription-3 (STAT3). (greenmedinfo.com)
  • Interleukin-10 combines with itself to form a homodimeric molecule that is the biologically active form of the protein. (curehunter.com)
  • The lyophilized IL-15 recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted IL-15 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing IL-15 recombinant protein to repeated freeze / thaw cycles is not recommended. (prosci-inc.com)
  • Recombinant human IL-15 is a 12.9 kDa protein consisting of 114 amino acid residues. (prosci-inc.com)
  • IL-15 binds to heterotrimeric receptors composed of IL-15Rα, IL-15Rβ, and IL-15Rγc. (biolegend.com)
  • BACKGROUND AND PURPOSE Interleukin-15 (IL-15) is important in the activation and proliferation of lymphocytic cell populations and is implicated in inflammatory disease. (immune-source.com)
  • and also potently inhibits IL-15 dependent proliferation of cells expressing IL-15R? (immune-source.com)
  • DISC0280 also inhibited the IL-15 dependent proliferation of M-07e cells that only express IL-15R? (immune-source.com)
  • Y510 efficiently mediated IL-21-induced phosphorylation of Stat1 and Stat3, but not of Stat5, and CD8(+) T cells from Stat1/Stat3 double knock-out mice exhibited decreased proliferation in response to IL-21 + IL-15. (nih.gov)
  • IL-15 stimulates the proliferation of activated T cells, NK cells, and B cells, inducing immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand. (biolegend.com)
  • Increased expression of IL-15 has been implicated with rheumatoid arthritis, inflammatory bowl disease and diseases affiliated with retroviruses HIV and HTLV-I. Human IL-15 is biologically active on mouse cells as measured by the dose-dependent stimulation of the proliferation of mouse CTLL cells. (prosci-inc.com)
  • It has been demonstrated that two isoforms of IL-15 mRNA are generated by alternative splicing in mice. (wikipedia.org)
  • We show that renal cell carcinoma cells and normal renal cells express IL-15 mRNA and membrane-bound IL-15 (MbIL-15). (nih.gov)
  • Thus, IL-15 induces chromatin remodeling of the IL12RB1 gene promoter, increasing IL12RB1 mRNA expression in synergy with IFN-γ through the recruitment of PU.1 and IRF3. (elsevierpure.com)
  • However, dysregulations of IL-15 expression could occur and participate in the emergence of autoimmune inflammatory diseases as well as hematological malignancies. (hal.science)
  • Description: Quantitative sandwich ELISA for measuring Chicken Interleukin 6 (IL-6) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. (elisastrip.com)
  • Serum interleukin-1 (IL-1) Level [Time Frame: At Baseline, and After 60 days of treatment. (who.int)
  • Like IL-2, IL-15 binds to and signals through a complex composed of IL-2/IL-15 receptor beta chain (CD122) and the common gamma chain (gamma-C, CD132). (wikipedia.org)
  • The originally identified isoform, with long signal peptide of 48 amino acids (IL-15 LSP) consisted of a 316 bp 5'-untranslated region (UTR), 486 bp coding sequence and the C-terminus 400 bp 3'-UTR region. (wikipedia.org)
  • The other isoform (IL-15 SSP) has a short signal peptide of 21 amino acids encoded by exons 4A and 5. (wikipedia.org)
  • IL-15 LSP isoform was identified in Golgi apparatus [GC], early endosomes and in the endoplasmic reticulum (ER). (wikipedia.org)
  • On the other hand, IL-15 SSP isoform is not secreted and it appears to be restricted to the cytoplasm and nucleus where plays an important role in the regulation of cell cycle. (wikipedia.org)
  • Interleukin-12 receptor β1 (IL12RB1) is expressed on a variety of immune cells, including T and natural killer (NK) cells and macrophages, and is involved in innate and adaptive immune responses. (elsevierpure.com)
  • IL-15 acts as a bridge between innate and adaptive immunity because of its diverse roles in the immune system. (biolegend.com)
  • The main mechanism of IL-15 signaling is trans-presentation which is mediated by membrane-bound complex IL-15/IL-15Rα (Figure 3). (wikipedia.org)
  • These findings suggest that IL-15 produced by renal tumor cells is involved in the maintenance of active NK cells at the tumor site. (nih.gov)
  • Dauber, Victoria (2018): Körperliche Leistungsfähigkeit, Diabetesrisiko und das Myokin Interleukin-6 bei Frauen nach Gestationsdiabetes. (uni-muenchen.de)
  • Natural killer cell stimulatory factor (interleukin 12 [IL-12]) induces T helper type 1 (T h 1)-specific immune responses and inhibits the development of IL-4-producing Th cells. (nature.com)
  • Notably, chromatin immunoprecipitation (ChIP) assays revealed IL-15 rapidly induced histone H3 acetylation at the IL12RB1 promoter. (elsevierpure.com)
  • Promoter analyses in a mouse macrophage cell line, RAW264.7, revealed that the 2508-bp region upstream of the transcriptional start Site is sufficient for the full transcriptional activation of the IL12RB1 gene by IFN-γ or IL-15. (elsevierpure.com)
  • IL-15 exerts its biological activites primarily on T cells. (prosci-inc.com)
  • IL-15 is crucial for the homeostasis of Natural Killer (NK) and memory CD8 T cells, and to fight against cancer progression. (hal.science)
  • In Sweden, there has been a steady prostate-specific antigen (PSA) velocity for the diagnosis of pros- decrease in age-standardized breast cancer mortality in women up tate cancer (15). (lu.se)
  • without an IgG1 Fc domain) as a complex with hIL-15 (Figure 4A column 4 (immune-source.com)
  • IL-15 is 14-15 kDa glycoprotein encoded by the 34 kb region of chromosome 4q31 in humans, and at the central region of chromosome 8 in mice. (wikipedia.org)
  • IL-15 is secreted by mononuclear phagocytes (and some other cells) following infection by virus(es). (wikipedia.org)
  • These cells also express IL-15 receptor alpha (IL-15Ralpha). (nih.gov)
  • Furthermore, we show that MbIL-15 from renal cell carcinoma cells is functional and involved in rapid nuclear translocation of phosphorylated signal transducers and activators of transcription 3 in IL-2-starved NK cells. (nih.gov)
  • MbIL-15 on the target did not interfere with resting NK cell activation and target cell cytolysis but rescued NK cells from IL-2 starvation-induced apoptosis through contact-dependent interaction. (nih.gov)
  • Hence, we established a two-phase expansion protocol with IL-15 to induce an early NK cell expansion, followed by short exposure to IL-21 that boosted the cytotoxic activity of NK cells against RMS cells. (frontiersin.org)
  • In a proof of concept in vivo study, we also observed a therapeutic effect of adoptively transferred IL-15 expanded and IL-21 boosted NK cells in combination with image guided high precision radiation therapy using a luciferase-transduced RMS xenograft model. (frontiersin.org)
  • IL-2 and IL-15 have similar impacts on NK cells ( 13 , 14 ). (frontiersin.org)
  • However, direct injection of IL-2 has been shown to be accompanied by severe side effects, such as vascular leak syndrome, activation-induced cell death, and strong induction of regulatory CD4 pos T cells, which did not occur after IL-15 administration ( 15 , 16 ). (frontiersin.org)
  • Human IL-15 injected into Emodin mice caused an increase in NK1.1+ and CD3+ cells in the spleen and peripheral blood and these effects were unexpectedly potentiated by giving DISC0280 with human IL-15. (immune-source.com)
  • Body 5 (A) Treatment of mice with B-E29 causes a dosage dependent reduction in the result of hIL-15 on NK1.1+ cells. (immune-source.com)
  • Targeting constitutive and interleukin-6-inducible signal transducers and activators of transcription 3 pathway in head and neck squamous cell carcinoma cells by curcumin (diferuloylmethane). (greenmedinfo.com)
  • Chamie and colleagues hypothesized that BCG plus interleukin-15 would increase NK and T-cell recruitment without upregulation of regulatory T cells, further augmenting response and improving DFS. (medpagetoday.com)
  • In this study production levels of interleukins (IL)-12 and IL-13 were measured by commercial ELISA in culture supernatants of mitogen-stimulated peripheral blood mononuclear cells from 30 non-splenectomized beta-thalassaemia cases with iron overload and 20 age- and sex-matched healthy individuals. (who.int)
  • IL-15 bind to IL-15Rα receptor alone with an affinity of Ka = 1.1011/M. It can also bind to IL-15Rβγc signaling complex with lower affinity (Ka = 1.109/M) (Figure 4). (wikipedia.org)
  • These observations raise the possibility that DISC0280 or equivalent antibodies could be used to substitute clinically Emodin for IL-15 Emodin where a specific immunostimulation is desirable. (immune-source.com)
  • However in an model of hIL-15 activity we also show an opposing action for DISC0280 highlighting the complexity of pursuing IL-15 as a therapeutic target. (immune-source.com)
  • Native mouse Interleukin-3 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 15kDa. (reprokine.com)
  • Our results show that maternal dietary n-3 PUFA deficiency increases microglia-mediated phagocytosis of synaptic elements in the rodent developing hippocampus, partly through the activation of 12/15-lipoxygenase (LOX)/12-HETE signaling, altering neuronal morphology and affecting cognitive performance of the offspring. (nature.com)