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  • gene
  • Transcription factor binding sites identified by Qiagen in the CABP1 gene promoter include Nkx2-5, RSRFC4, TAL1, and HSF1. (wikipedia.org)
  • IP3R
  • Western blotting using a polyclonal antibody directed against purified brain IP3 receptor (IP3R) identified a specific 260 kD band in 1% Triton X-100 extracts of rat, hamster, mouse and dog sperm. (rupress.org)
  • In mouse eggs, these oscillations cease after a variable period of time and this is accompanied by a decrease in inositol 1,4,5-trisphosphate receptor (IP3R) responsiveness and down-regulation of the IP3R type 1 (IP3R-1). (nih.gov)
  • To investigate the signaling pathway responsible for inducing IP3R-1 down-regulation during fertilization, mouse eggs were exposed to or injected with several Ca(2+)-releasing agonists and the amounts of IP3R-1 immunoreactivity evaluated by Western blotting. (nih.gov)
  • Exposure to ethanol or ionomycin, which induce a single [Ca(2+)]i rise, failed to signal down-regulation of IP3R-1. (nih.gov)
  • However, [Ca(2+)]i oscillations induced by injection of boar sperm fractions (SF), which presumably stimulate production of IP3, or adenophostin A, an IP3R agonist, both induced down-regulation of IP3R-1 of a magnitude similar to or greater than that observed after fertilization. (nih.gov)
  • Exposure to thimerosal, an oxidizing agent that modifies the IP3R without stimulating production of IP3, also initiated down-regulation of IP3R-1, although oscillations initiated by SrCl(2) failed to evoke down-regulation of IP3R-1. (nih.gov)
  • The degradation of IP3R-1 in mouse eggs appears to be mediated by the proteasome pathway because it was inhibited by preincubation with lactacystin, a very specific proteasome inhibitor. (nih.gov)
  • We therefore suggest that persistent stimulation of the phosphoinositide pathway in mouse eggs by the sperm during fertilization or by injection of SF leads to down-regulation of the IP3R-1. (nih.gov)
  • InsP
  • This quantal pattern of Ca 2+ release occurred both at 25 °C and at 4 °C. There was a time-dependent increase in the fraction of Ca 2+ recruited by the lower compared with the higher [InsP 3 ]. (biochemj.org)
  • Pancreatic acinar cells are highly polarized, both functionally and morphologically and InsP 3 Rs are predominantly expressed in ER extensions in the apical regions juxtaposed to the acinar lumen ( Figure 1 ) ( 34 , 36 , 42 , 69 ). (pancreapedia.org)
  • inhibition
  • We compared the Ins(1,4,5)P3-binding sites of liver and cerebellum by measuring inhibition of specific Ins(1,4,[32P]5)P3 binding by various ligands under equilibrium conditions, and find that each ligand binds with similar affinity in the two tissues. (biochemj.org)
  • Schild analysis of inhibition of Ins(1,4,5)P3 binding by ATP demonstrates a competitive interaction between the two at the liver Ins(1,4,5)P3-binding site, and this partly accounts for earlier discrepancies in binding and Ca2(+)-release data. (biochemj.org)
  • Enhances Ca(2+)-mediated inhibition of inositol 1,4,5-triphosphate receptor (ITPR) Ca(2+) release. (mybiosource.com)
  • PPY - inhibition of upper GI tract motility GLP-1 - Functions as an "Ileal Brake" to inhibit upper GI tract motility when the distal gut is exposed to unabsorbed nutrients. (wikipedia.org)
  • phospholipase
  • Peptide antibodies colocalized G alpha q/11 and the beta 1 isoform of phospholipase C (PLC beta 1) to the anterior acrosomal region of mouse sperm. (rupress.org)
  • human
  • The Ins(1,4,5)P3 receptor was examined in human promyelocytic leukaemic cells (HL-60) and in HL-60 cells differentiated towards granulocytes with either retinoic acid (RA) or dimethyl sulphoxide (Me2SO). (biochemj.org)
  • Subcellular fractionation of SH-SY5Y human neuroblastomas revealed that the type 2 RyR and type 1 Ins(1,4,5) P 3 R co-localize among the fractions of a sucrose-cushion separation of crude microsomal membrane fractions. (biochemj.org)
  • The helical domain is juxtaposed against one end of the cleft in the large domain There are three Inositol Trisphosphate 3 Kinases which are encoded by the human genome. (wikipedia.org)
  • Ryanodine
  • 2011) have demonstrated oxygen-coupled redox regulation of the skeletal muscle ryanodine receptor-Ca2+ release channel (RyR1;TC# 1.A.3.1.2) by NADPH oxidase 4. (wikipedia.org)
  • mobilization
  • Earlier studies in which Ins(1,4,5)P3 binding and Ca2+ mobilization were measured under different conditions demonstrated large differences between KD values for binding and EC50 values (concn. (biochemj.org)
  • We conclude that the high-affinity Ins(1,4,5)P3-binding site of hepatocytes is likely to be the receptor that mediates Ca2+ mobilization, and that this receptor is at present indistinguishable from that in cerebellum. (biochemj.org)
  • These data indicate that, provided that inositol 1,4,5-trisphosphate is studied independently of inositol 1,3,4-trisphosphate, the former shows metabolic characteristics consistent with its proposed role as a second messenger for calcium mobilization. (biochemj.org)
  • neurons
  • Type 1 IP 3 R (IP 3 R1) is enriched in growth cones of neurons in chick dorsal root ganglia. (sciencemag.org)
  • type
  • Manipulation of SH-SY5Y cells by chronic stimulation of muscarinic acetylcholine receptor (mAChR) results in a decrease in their type 1 Ins(1,4,5) P 3 R levels but not in the abundance of the type 2 RyR. (biochemj.org)
  • involves
  • This bond involves combining a hydroxyl group from the inositol ring and a free phosphate group through a dehydration reaction. (wikipedia.org)
  • reaction
  • The first reaction is the oxidation of glyceraldehyde 3-phosphate (G3P) at the position-1 (in the diagram it is shown as the 4th carbon from glycolysis), in which an aldehyde is converted into a carboxylic acid (ΔG°'=-50 kJ/mol (−12kcal/mol)) and NAD+ is simultaneously reduced endergonically to NADH. (wikipedia.org)
  • The energy released by this highly exergonic oxidation reaction drives the endergonic second reaction (ΔG°'=+50 kJ/mol (+12kcal/mol)), in which a molecule of inorganic phosphate is transferred to the GAP intermediate to form a product with high phosphoryl-transfer potential: 1,3-bisphosphoglycerate (1,3-BPG). (wikipedia.org)
  • release
  • The inositol 1,4,5-trisphosphate (IP 3 ) receptor (IP 3 R) acts as a Ca 2+ release channel on internal Ca 2+ stores. (sciencemag.org)
  • Finally, a molecule of inorganic phosphate attacks the thioester and forms a tetrahedral intermediate, which then collapses to release 1,3-bisphosphoglycerate, and the thiol group of the enzyme's cysteine residue. (wikipedia.org)
  • structure
  • When HL-60 cells are incubated with 1 microM-RA or with 1.25% Me2SO the cells differentiate within 5-7 days into cells resembling neutrophils in both structure and function. (biochemj.org)
  • conversion
  • As its name indicates, glyceraldehyde 3-phosphate dehydrogenase (GAPDH) catalyses the conversion of glyceraldehyde 3-phosphate to D-glycerate 1,3-bisphosphate. (wikipedia.org)
  • cell
  • Concomitant with HL-60 cell differentiation, the maximal [3H]Ins(1,4,5)P3 binding in membranes increases 3-4-fold, with no change in KD. (biochemj.org)
  • receptors
  • In the present study, to clarify this possible relationship, we investigated how calcium signaling and its associated purinergic receptors are related to the cell cycle between the nucleoplasm and cytoplasm in cultured G 1 -interphase cells of HeLa.S-Fucci2 and fucci/mouse fibroblasts. (go.jp)
  • Ratiometric fluorescence and reverse transcriptase polymerase chain reaction (RT-PCR) techniques were employed to assess the intracellular Ca 2+ concentrations ([Ca 2+ ] i ) and the expression of purinergic and inositol trisphosphate receptors, respectively. (go.jp)
  • In addition to the Ca 2+ wave, the RT-PCR results indicated variability in the purinergic receptors and inositol 1,4,5-trisphosphate receptor subtypes within G 1 -phase cells. (go.jp)
  • While extracellular Ca 2+ seems to enter the cytoplasm upon 6-OH-BDE-47 by interfering with the cell membrane and independent of Ca 2+ ion channels, ER-derived Ca 2+ is released following activation of protein lipase C and inositol 1,4,5-trisphosphate receptor, but independently of ryanodine receptors. (springer.com)
  • Parathyroid hormone (PTH) stimulates formation of cAMP and sensitizes inositol 1,4,5-trisphosphate receptors (IP(3)R) to IP(3). (nih.gov)
  • 4 Subsequent studies implicate weak coupling of the prostanoid receptors to stimulatory G s proteins and the cAMP pathway in young SHR. (ahajournals.org)
  • 6 The SHR responses to stimulation of the vascular AVP V 1 receptors are exaggerated whether or not indomethacin is administered to inhibit cyclooxygenase and prostaglandin production. (ahajournals.org)
  • Humans
  • The current study shows that BAs elicit a specific response in humans in which they repress hypoxia-inducible factor 1? (jove.com)
  • Humans contain two members, TPTE and TPTE2, which result from a primate-specific duplication [1]. (wikipedia.org)
  • This belief was taken apart when it became clear that only few of the ejaculated spermatozoa - in humans, only ~1 of every million spermatozoa - succeed in entering the oviducts (Fallopian tubes) and when more recent studies showed that mammalian spermatozoa do respond chemotactically. (wikipedia.org)

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  • phosphatases
  • These include enzymes called ceramide-activated Ser-Thr phosphatases (CAPPs), such as protein phosphatase 1 and 2A (PP1 and PP2A), which were found to interact with ceramide in studies done in a controlled environment outside of a living organism (in vitro). (wikipedia.org)
  • activates
  • The chemicals 2-(2,3-dicarboxycyclopropyl)glycine (DCG-IV) and eglumegad activate only group II mGluRs, while 2-amino-4-phosphonobutyrate (L-AP4) activates only group III mGluRs. (wikipedia.org)
  • The current knowledge is mainly based on studies in the sea urchin Arbacia punctulata, where binding of the chemoattractant resact (Table 1) to its receptor, a guanylyl cyclase, activates cGMP synthesis (Figure 1). (wikipedia.org)
  • proteins
  • So far, most sperm chemoattractants that have been identified in non-mammalian species are peptides or low-molecular-weight proteins (1-20 kDa), which are heat stable and sensitive to proteases. (wikipedia.org)
  • Molecular
  • The suppression of HIF-1 signaling by bile acids may have a significant influence on the progression and outcome of respiratory disease, and the molecular mechanism underpinning this response warrants further investigation. (jove.com)
  • isoform
  • A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain. (abcam.cn)
  • Isoform 1 has high affinity for FGF1 and FGF2 , but low affinity for FGF7 . (rcsb.org)
  • Isoform 3 has high affinity for FGF1 and FGF7 , and has much higher affinity for FGF7 than isoform 1 (in vitro). (rcsb.org)
  • second
  • Thus, the abnormal effects of AVP on renal resistance vessels of SHR are probably mediated by a strain difference in V 1 receptor function, or postreceptor second-messenger systems, or both. (ahajournals.org)
  • RYR1
  • FKBP1A has been shown to interact with: GLMN, ITPR1 KIAA1303, Mammalian target of rapamycin, RYR1, and TGF beta receptor 1. (wikipedia.org)
  • 2017
  • Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017! (bosterbio.com)
  • sperm
  • Exceptions to this rule are the sperm chemoattractants of corals, ascidians, plants such as ferns, and algae (Table 1). (wikipedia.org)
  • stores
  • 2003). In HEK-PR1 cells, PTH caused modest phosphorylation of IP3R (Figs. 3 A and S2 B, available at http://www.jcb.org/cgi/content/full/jcb.200803172/DC1), and PKA very slightly (though not to a statistically significant degree) increased the sensitivity of the intracellular Ca2+ stores to IP3 (see Fig. 4 A). (nih.gov)