Loading...
  • morphology
  • The functions of Myc-nick are currently under investigation, but this new Myc family member was found to regulate cell morphology, at least in part, by interacting with acetyl transferases to promote the acetylation of α-tubulin. (wikipedia.org)
  • KLF4
  • The three transcription factors Oct3/4, Sox2 and Klf4 are essential for reprogramming but are extremely inefficient on their own, fully reprogramming roughly only 0.005% of the number of cells treated with the factors. (wikipedia.org)
  • germ cells
  • After fertilization some cells of the newly formed embryo migrate to the germinal ridge and will eventually become the germ cells (sperm and oocytes). (wikipedia.org)
  • Therefore, during the process of gametogenesis the primordial germ cells must have their original biparental DNA methylation patterns erased and re-established based on the sex of the transmitting parent. (wikipedia.org)
  • mammalian
  • Ben-Nun I.F., Montague S.C., Houck M.L., Ryder O., Loring J.F. (2015) Generation of Induced Pluripotent Stem Cells from Mammalian Endangered Species. (springer.com)
  • In biology, Reprogramming refers to erasure and remodeling of epigenetic marks, such as DNA methylation, during mammalian development or in cell culture. (wikipedia.org)
  • Ian Wilmut and Keith Campbell were the first to demonstrate that an adult mammalian cell could be reprogrammed back into a pluripotent state when they cloned Dolly the sheep in 1997. (wikipedia.org)
  • autologous
  • The reprogramming method paves a pathway for autologous and allogeneic cell therapy that satisfies regulatory requirements. (eurekalert.org)
  • neurons
  • LRRK2 mutant iPSC-derived da neurons demonstrate increased susceptibility to oxidative stress," Cell Stem Cell , vol. 8, no. 3, pp. 267-280, 2011. (hindawi.com)
  • Because they can propagate indefinitely, as well as give rise to every other cell type in the body (such as neurons, heart, pancreatic, and liver cells), they represent a single source of cells that could be used to replace those lost to damage or disease. (wikipedia.org)
  • CDI offers several terminally differentiated cell types as catalog products: iCell® Cardiomyocytes, iCell Neurons, iCell Endothelial Cells, and iCell Hepatocytes. (wikipedia.org)
  • Physiology
  • For this research coup and his leading role pioneering stem cell work, Yamanaka - who holds academic appointments at Kyoto University and UC San Francisco - was the co-recipient of the 2012 Nobel Prize in Medicine or Physiology . (ucsf.edu)
  • The transcription factor c-Myc can also be used as the fourth factor and was the original fourth factor used by Shinya Yamanaka who received the 2012 Nobel Prize in Physiology or Medicine for his work in the conversion of somatic cells to iPS cells. (wikipedia.org)
  • These tissues provide a unique in vitro model to study cardiac physiology with a species-specific advantage over cultured animal cells in experimental studies. (wikipedia.org)
  • tissue
  • Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution," Cell Stem Cell , vol. 1, no. 1, pp. 55-70, 2007. (hindawi.com)
  • To compare and contrast mature connective tissue cells called fibroblasts with the pluripotent stem cells into which they were reprogrammed, the investigators focused on a chemical change known as methylation. (redorbit.com)
  • Induced pluripotent stem cells that were reprogrammed from normal adult human tissue and have not yet been differentiated. (ucsf.edu)
  • As well, he was one of the first two animals of an endangered species to have his tissue samples used to make induced pluripotent stem cells as an attempt at conservation. (wikipedia.org)
  • In the post-implantation stage, methylation patterns are stage- and tissue-specific with changes that would define each individual cell type lasting stably over a long time. (wikipedia.org)
  • Factors leading to variation in the performance of reprogramming and functional features of end products include genetic background, tissue source, reprogramming factor stoichiometry and stressors related to cell culture. (wikipedia.org)
  • SCD-1 expression is significantly increased in liver tissue and heart in response to a high-carbohydrate diet, whereas SCD-2 expression is observed in brain tissue and induced during the neonatal myelination. (wikipedia.org)
  • His lab work is conducted on mice, induced pluripotent stem cells (iPSC) and Human postmortem tissue. (wikipedia.org)
  • Cellular
  • This type of research gets to the fabric of the fundamental differences between stem cells and their parental cells," says Akiko Doi, a doctoral candidate in the graduate program in Cellular and Molecular Medicine at Johns Hopkins. (redorbit.com)
  • Thus, the ability to switch states/phenotypes (phenotypic plasticity) is a key feature of development and normal function of cells within most multicellular organisms that enables the cell to respond to various intrinsic and extrinsic cues and stimuli in a concerted fashion enabling them to 'make' appropriate cellular decisions. (wikipedia.org)
  • Cellular Dynamics International (CD) was founded in 2004 by pluripotent stem cell biologist, James Thomson. (wikipedia.org)
  • Human cells are considered to be a more predictive model and a replacement for other cellular models that serve as proxies, including animal cells, immortalized cell lines, and cadaveric cells. (wikipedia.org)
  • The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. (wikipedia.org)
  • Jaiswal, Manoj K. (2012), Motoneuron specific calcium dysregulation and perturbed cellular calcium homestasis in amyotrophic lateral sclerosis: recent advances gained from genetically modified animals and cell culture models. (wikipedia.org)
  • research
  • The research team transformed tumor-generating cell types into iPS-like cells by manipulating their genetic makeup. (redorbit.com)
  • Human stem cell research is a new field with much promise, but progress towards a clinical setting has been complicated by scientific and ethical challenges. (jhu.edu)
  • The most heated discussion over stem cell research has focused on the source of human embryonic stem cells (ESCs). (jhu.edu)
  • I argue that a new approach is needed to deal with the serious uncertainties and indeterminate probabilities intrinsic to stem cell research and decision-making surrounding it. (jhu.edu)
  • I conclude that in the current state of knowledge, stem cell research needs to continue and be allowed to unfold as the narrative of a story. (jhu.edu)
  • Robinton DA, Daley GQ (2012) The promise of induced pluripotent stem cells in research and therapy. (springer.com)
  • Only then will we be confident that iPS cells are a safe replacement for ES cells in research and therapy. (redorbit.com)
  • Their research results were published in the October issue of Stem Cells. (innovations-report.com)
  • Research and emerging treatments with stem cells today can be traced to a startling discovery 10 years ago when Shinya Yamanaka , MD, PhD, and his graduate student Kazutoshi Takahashi , PhD, reported a way to reprogram adult mouse cells and coax them back to their embryonic state - pluripotent stem cells. (ucsf.edu)
  • Shantanu Sengupta is an Indian cell biologist and a professor at the Institute of Genomics and Integrative Biology (IGIB) of the Council of Scientific and Industrial Research. (wikipedia.org)
  • Current research directions include determining the exact mechanism of the disease, improving animal models to aid with research, testing of medications to treat symptoms or slow the progression of the disease, and studying procedures such as stem cell therapy with the goal of repairing damage caused by the disease. (wikipedia.org)
  • familial
  • Study familial hypertrophic cardiomyopathy using patient-specific induced pluripotent stem cells. (nih.gov)
  • Dr. Jaiswal studies the critical role of Cu, Zn superoxide dismutase (SOD1), typical for familial ALS, in the impairment of [Ca2+]mito handling and perturbation of Ca2+ homeostasis in SOD1G93A mice and cell culture models of ALS. (wikipedia.org)
  • epigenetic
  • Chou BK, Mali P, Huang X et al (2011) Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures. (springer.com)
  • Relatively little study has been done on the epigenetic nature of stem cells," says Andrew Feinberg, M.D., M.P.H., a professor of medicine at the Johns Hopkins University School of Medicine. (redorbit.com)
  • If we can use the epigenetic information to characterize these cells, this could inform how we might use them therapeutically. (redorbit.com)
  • However, the molecular makeup of these cells, including gene expression and epigenetic marks, was somewhere between that of a fibroblast and an ESC, and the cells failed to produce viable chimeras when injected into developing embryos. (wikipedia.org)
  • scientists
  • Both types of stem cells are well known by scientists to have the propensity to cause teratomas, a benign tumor that consists of many different cell types. (redorbit.com)
  • The scientists contrasted the different cells' transcriptomes, comprised of the RNA molecules or "transcripts. (redorbit.com)
  • As scientists learn more about the epigenetics of reprogrammed cells, they may find new ways of creating them or using them. (redorbit.com)
  • ESCs
  • The pluripotent cell types that comprise embryoid bodies include embryonic stem cells (ESCs) derived from the blastocyst stage of embryos from mouse (mESC), primate, and human (hESC) sources. (wikipedia.org)
  • In contrast to monolayer cultures, however, the spheroid structures that are formed when ESCs aggregate enables the non-adherent culture of EBs in suspension, making EB cultures inherently scalable, which is useful for bioprocessing approaches, whereby large yields of cells can be produced for potential clinical applications. (wikipedia.org)
  • Additionally, although EBs largely exhibit heterogeneous patterns of differentiated cell types, ESCs are capable of responding to similar cues that direct embryonic development. (wikipedia.org)
  • In addition, technologies have also been developed to physically separate cells by forced aggregation of ESCs within individual wells or confined on adhesive substrates, which enables increased throughput, controlled formation of EBs. (wikipedia.org)
  • colonies
  • Traditionally, hiPSCs are expanded in two-dimensional static culture as colonies in the presence or absence of feeder cells. (springer.com)
  • Alternatively, to avoid dissociation into single cells, EBs can be formed from hESCs by manual separation of adherent colonies (or regions of colonies) and subsequently cultured in suspension. (wikipedia.org)
  • neuronal
  • Hyperexcitability is considered to be a hallmark of ALS, and it has been suggested that the ALS-associated hyperexcitability may stem from altered function of the neuronal glutamate receptors due to inefficient RNA editing of one of the receptor's subunits. (wikipedia.org)
  • inhibitors
  • As hESC undergo apoptosis when cultured as single cells, EB formation often necessitates the use of inhibitors of the rho associated kinase (ROCK) pathway, including the small molecules Y-27632 and 2,4 disubstituted thiazole (Thiazovivin/Tzv). (wikipedia.org)
  • models
  • Reconciling computer models and stem cell models of human cardiac repolarization: reply. (nih.gov)
  • New drugs tested on human cells instead of animal models. (ucsf.edu)
  • It also allowed researchers to screen new drugs directly in human cells rather than relying on animal models, which more often than not fail to accurately predict a new drug's effects on people. (ucsf.edu)
  • iPSC-derived
  • LRRK2 mutations cause mitochondrial DNA damage in iPSC-derived neural cells from Parkinson's disease patients: reversal by gene correction," Neurobiology of Disease , vol. 62, pp. 381-386, 2014. (hindawi.com)
  • gene
  • This previously unidentified progenitor occurs as a temporally discrete population during ES cell differentiation, and differs from the preceding and succeeding populations in gene expression and differentiation potential, with the unique ability to form surface ectoderm in response to BMP4 signalling. (biologists.org)
  • Research applications such as gene editing, gene expression, and directed differentiation depend on the effective, efficient delivery of DNA, RNA and/or Cas9 protein complexes into stem cells. (thermofisher.com)
  • For gene editing, Lipofectamine Stem reagent allows the co-transfection of Cas9 protein complexed with guide RNAs along with single-stranded DNA for homology directed repair. (thermofisher.com)
  • In addition, Lipofectamine Stem reagent can be used for the transfection of mRNA for gene expression or directed differentiation studies using stem cells. (thermofisher.com)
  • The major emphasis of my research will be to employ induced Pluripotent Stem Cells and gene focusing on systems for your treatment of sufferers with inherited skin blistering conditions. (thezenweb.com)
  • The Regenerative Neurobiology Laboratory experiments novel mechanisms of neurological Restoration soon after gene therapy, stem cell transplant and organelle transfer pursuing world wide cerebral ischemia. (thezenweb.com)
  • The clock normally modulates expression of this gene that encodes an important cell cycle regulator, and that cell cycle regulator in turn affects not only the cell cycle but also the clock. (rxpgnews.com)
  • Promoter CpG methylation contributes to ES cell gene regulation in parallel with Oct4/Nanog, PcG complex, and histone H3 K4/K27 trimethylation," Cell Stem Cell , vol. 2, no. 2, pp. 160-169, 2008. (hindawi.com)
  • During the blastula stage of development, a significant amount of activity occurs within the early embryo to establish cell polarity, cell specification, axis formation, and regulate gene expression. (wikipedia.org)
  • Following λ-red operon activity induction, a linear, double-stranded cassette encoding a selectable marker, such as antibiotic resistance, is transformed into the cells in place of the target gene and incorporated into the DNA behind a specific inducible promoter. (wikipedia.org)
  • A plasmid expressing flippase (FLP) can be transformed into the recombined cells, which can specifically cleave FLP recognition target sites (FRTs) flanking the antibiotic resistance gene. (wikipedia.org)
  • Plant miRNAs usually have near-perfect pairing with their mRNA targets, which induces gene repression through cleavage of the target transcripts. (wikipedia.org)
  • The germ plasm effectively turns off gene expression to render the genome of the cell inert. (wikipedia.org)
  • Developmental biology and embryology provides the basic knowledge of the cell types' differentiation through mutation analysis, lineage tracing, embryo micro-manipulation and gene expression studies. (wikipedia.org)
  • Proper differentiation of the cell type of interest is verified by analyzing cell type specific markers, gene expression profile, and functional assays. (wikipedia.org)
  • Mice
  • 8.Yueli Wang, Yulin Li, Yina Wu, Lixin Jia, Jijing Wang, Bo Xie, Mizhou Hui, Jie Du: TNF-α and IL-1β Neutralization Ameliorates Angiotensin II-Induced Cardiac Damage in Male Mice. (54doctor.net)
  • After modifying the technique, Obokata was able to show that white blood cells from newborn mice could be transformed into cells that behaved much like stem cells. (wikipedia.org)
  • Her work focused on using pluripotent stem cells to create lung epithelial tissue in mice, and examining the potential effects of these results in human medicine. (wikipedia.org)
  • The CystLTs show relative potencies of LTE4>LTC4>LTD4 in stimulating GPR99-bearing cells with GPR99-deficient mice exhibiting a dose-dependent loss of vascular permeability responses in skin to LTE4 but not to LTC4 or LTD4. (wikipedia.org)
  • they found that GPR17-bearing cells did not respond to these CysLTs or nucleotides but did find that cells expressing both CysLTR1 and GPR17 receptors exhibited a marked reduction in binding LTC4 and that mice lacking GPR17 were hyper-responsive to igE-induced passive cutaneous anaphylaxis. (wikipedia.org)
  • Subsequently, in the future, it might be possible to specifically mature the cells into specific cells such as neurons and muscle cells, in a similar way in which Katsuhiko Hayashi has grown mice out of simple skin cells. (wikipedia.org)
  • embryo
  • Adding the two chemicals to the mix induced the cells to form tissue that genetic analysis revealed was very similar to actual developing salivary glands in the embryo. (brightsurf.com)
  • A series of experiments, first turning a mouse embryo green by fluorescently tagging STAP cells, then videotaping the transformation of T-cells into pluripotent cells, finally convinced skeptics that the results were real. (wikipedia.org)
  • France prohibits reproductive cloning and embryo creation for research purposes, but enacted laws (with a sunset provision expiring in 2009) to allow scientists to conduct stem cell research on imported a large amount of embryos from in vitro fertilization treatments. (wikipedia.org)
  • Rossant has discovered information on embryo development, how multiple types of stem cells are established, and the methods at which genes control development. (wikipedia.org)
  • The lab specifically focuses on how cells in the early mouse embryos decide their fate and how this information can be applied to maintaining and differentiating embryo-derived stem cells. (wikipedia.org)
  • Embryo development begins with a sperm fertilizing an egg to become a zygote which undergoes many cleavages to develop into a ball of cells called a morula. (wikipedia.org)
  • The addition of the two growth phases into the cell cycle allows for the cells to increase in size, as up to this point the blastomeres undergo reductive divisions in which the overall size of the embryo does not increase, but more cells are created. (wikipedia.org)
  • In genetics and developmental biology, somatic cell nuclear transfer (SCNT) is a laboratory strategy for creating a viable embryo from a body cell and an egg cell. (wikipedia.org)
  • Development will ensue normally and after many mitotic divisions, this single cell forms a blastocyst (an early stage embryo with about 100 cells) with an identical genome to the original organism (i.e. a clone). (wikipedia.org)
  • Stem cells can then be obtained by the destruction of this clone embryo for use in therapeutic cloning or in the case of reproductive cloning the clone embryo is implanted into a host mother for further development and brought to term. (wikipedia.org)
  • The aim of carrying out this procedure is to obtain pluripotent cells from a cloned embryo. (wikipedia.org)
  • citation needed] In the mouse, primordial germ cells (PGCs) arise in the posterior primitive streak of the embryo and start to migrate around 6.25 days after conception. (wikipedia.org)
  • Mammalian PGCs are specified by signalling between cells (induction), rather than by the segregation of germ plasm as the embryo divides. (wikipedia.org)
  • After fertilization some cells of the newly formed embryo migrate to the germinal ridge and will eventually become the germ cells (sperm and oocytes). (wikipedia.org)
  • During differentiation, pluripotent cells make a number of developmental decisions to generate first the three germ layers (ectoderm, mesoderm and endoderm) of the embryo and intermediate progenitors, followed by subsequent decisions or check points, giving rise to all the body's mature tissues. (wikipedia.org)
  • mRNA
  • (B) Human ES (H9) cells were transfected with Lipofectamine Stem Reagent or FuGENE HD Reagent according to the protocol with an mRNA expressing GFP. (thermofisher.com)
  • C, left) Lipofectamine Stem Reagent was used to transfect cells with Cas9 mRNA (modified)/ gRNA: Emx-1 crRNA (Exon2)-tracrRNA oligo/ GFP mRNA (modified) or Cas9 protein/gRNA: Emx-1 crRNA (Exon2)-tracrRNA oligo/ GFP mRNA (modified). (thermofisher.com)
  • These genes play a role in germ line development to localize nanos mRNA to the posterior and localize germ cell determinants. (wikipedia.org)
  • neural
  • In Xenopus laevis , BMP4 has been shown to suppress neural tissue and induce surface ectoderm from the definitive ectoderm ( Wilson and Hemmati-Brivanlou, 1995 ). (biologists.org)
  • Probably involved in the regulatory networks that define neural crest cell fate specification and determine mesoderm cell lineages in mammals. (genecards.org)
  • In striking contrast to these studies, studies concentration on neural tissues continue to find that Oligodendrocyte progenitor cells express GPR17 and respond through this receptor to LTC4, LTD4, and certain purines (see GPR17#Function). (wikipedia.org)
  • International Society for Stem Cell Res
  • In 2013, she was the president of International Society for Stem Cell Research. (wikipedia.org)
  • She is also a member or life member of the Indian Society of Cell Biology, the Society for Developmental Biology, USA, the International Society for Stem Cell Research (ISSCR), the North American Vascular Biology Organization (NAVBO) and the Indian Society of Developmental Biologists. (wikipedia.org)
  • ectoderm
  • Using a previously established mouse ES-cell-based system that recapitulates the development of the ectoderm lineage we have identified a transient population that is consistent with definitive ectoderm. (biologists.org)
  • The ability to form, recognize and manipulate the differentiation of definitive ectoderm is crucial to understanding the determination of cell fate in the ectoderm. (biologists.org)
  • and predicts formation of a cell population equivalent to the definitive ectoderm. (biologists.org)
  • subsequently
  • The technique for producing STAP cells was subsequently studied by Obokata at the Brigham and Women's Hospital (BWH), while she was studying as a post doc under Charles Vacanti, and then at the RIKEN Center for Developmental Biology in Japan. (wikipedia.org)
  • cleavage
  • Prior to this transition, cleavage occurs with only the synthesis and mitosis phases of the cell cycle. (wikipedia.org)
  • In contrast, animal miRNAs are able to recognize their target mRNAs by using as little as 6-8 nucleotides (the seed region) at the 5' end of the miRNA, which is not enough pairing to induce cleavage of the target mRNAs. (wikipedia.org)
  • The cells that give rise to the gametes are often set aside during embryonic cleavage. (wikipedia.org)
  • Cleavage in most animals segregates cells containing germ plasm from other cells. (wikipedia.org)
  • biology
  • The international journal of biochemistry & cell biology.2014;55:65-71. (54doctor.net)
  • Rossant has won numerous awards in cell and developmental biology. (wikipedia.org)
  • The SB ("Sleeping Beauty") 100X is announced as the Molecule of the Year 2009 by Isidro A. T. Savillo, President of the International Society for Molecular and Cell Biology and Biotechnology Protocols and Researches (ISMCBBPR). (wikipedia.org)
  • In biology, Reprogramming refers to erasure and remodeling of epigenetic marks, such as DNA methylation, during mammalian development or in cell culture. (wikipedia.org)
  • Directed differentiation is a bioengineering methodology at the interface of stem cell biology, developmental biology and tissue engineering. (wikipedia.org)
  • Efficient directed differentiation requires a detailed understanding of the lineage and cell fate decision, often provided by developmental biology. (wikipedia.org)
  • progenitor
  • We examine how competitive dynamics in stem and progenitor cell compartments influence irrespective of whether initiating oncogenic occasions bring about clonal growth and most cancers. (thezenweb.com)
  • transcription factors
  • STAP would have been a radically simpler method of stem cell generation than previously researched methods as it requires neither nuclear transfer nor the introduction of transcription factors. (wikipedia.org)
  • Reprogramming can also be induced artificially through the introduction of exogenous factors, usually transcription factors. (wikipedia.org)
  • This method, also known as transdifferentiation or direct conversion, consists in overexpressing one or several factors, usually transcription factors, introduced in the cells. (wikipedia.org)
  • A drawback of this approach is the introduction of foreign nucleic acid in the cells and the forced expression of transcription factors which effects are not fully understood. (wikipedia.org)
  • protein
  • 18.Shu-Lan Qiu, Zhi-Cheng Xiao, Chun-Mei Piao, Ying-Lin Xian, Li-Xin Jia, Young-Fen Qi, Jiahuai Han, You-Yi Zhang, Jie Du: AMP-Activated Protein Kinase α2 Protects Against Liver Injury from Metastasized Tumors via Reduced Glucose Deprivation-Induced Oxidative Stress. (54doctor.net)
  • 631166 and 631346), or you can monitor co-induced expression of your transgene with a fluorescent protein using kits that contain pTRE3G-mCherry (Cat. (clontech.com)
  • Alkaline phosphatase in E. coli is located in the periplasmic space and can thus be released using techniques that weaken the cell wall and release the protein. (wikipedia.org)
  • CysLTR1 is a G protein-coupled receptor that links to and when bound to its CysLT ligands activates the Gq alpha subunit and/or Ga subunit of its coupled G protein, depending on the cell type. (wikipedia.org)
  • populations
  • Recognition and analysis of these transient cell populations has been hampered by technical difficulties in accessing them in vivo. (biologists.org)
  • In this regard, the most promising strategy is the selective chemical ablation of undifferentiated cells in PSC-derived populations using small molecules which are not toxic to differentiated cells of interest. (provendis.info)
  • Inflammation
  • 15.Guan-Ming Qi, Li-Xin Jia, Yu-Lin Li, Hui-Hua Li, Jie Du: Adiponectin Suppresses Angiotensin II-Induced Inflammation and Cardiac Fibrosis through Activation of Macrophage Autophagy. (54doctor.net)
  • 1998
  • In 1998, her work helped lead to the discovery of the trophoblast stem cell, which has assisted in showing how congenital anomalies in the heart, blood vessels, and placenta can occur. (wikipedia.org)
  • genome
  • In this system, the red operon from bacteriophage λ is transfected into E. coli cells to facilitate incorporation of linear target DNA into the E. coli genome. (wikipedia.org)
  • primordial germ
  • Cells expressing germ plasm become primordial germ cells (PGCs) which will then give rise to the gametes. (wikipedia.org)
  • This cytoplasm, pole plasm, contains specialized materials called polar granules and the pole cells are the precursors to primordial germ cells. (wikipedia.org)
  • Therefore, during the process of gametogenesis the primordial germ cells must have their original biparental DNA methylation patterns erased and re-established based on the sex of the transmitting parent. (wikipedia.org)
  • fundamental
  • It suggests a fundamental connection among circadian timing, cell cycle progress, and potentially the origins of some cancers. (rxpgnews.com)
  • Maintaining homeostatic Ca 2+ signaling is a fundamental physiological process in living cells. (jove.com)
  • types
  • This means that in these cell types long term tetracycline inducible expression can be achieved. (clontech.com)
  • She repeated the experiment with other cell types including brain, skin, and muscle cells with the same result. (wikipedia.org)
  • novel
  • The notion that the clock regulates DNA-damage input and that mutation can affect the clock as well as the cell cycle is novel. (rxpgnews.com)
  • RIKEN
  • On August 5, 2014, Yoshiki Sasai-Obokata's supervisor at RIKEN and one of the coauthors on the STAP cell papers-was found dead at a RIKEN facility after an apparent suicide by hanging. (wikipedia.org)
  • proteins
  • Recent evidence in mammalian cells shows that other cell cycle regulators physically interact with clock proteins. (rxpgnews.com)
  • These IRES-containing bicistronic vectors allow the simultaneous induced expression of two proteins separately but from the same RNA transcript. (clontech.com)
  • Phys.org) 4 April - A new method developed by Cornell biological engineers offers an efficient way to make proteins for use in medicine or industry without the use of live cells. (wikipedia.org)
  • Alkaline phosphatase in E. coli is uncommonly soluble and active within elevated temperature conditions such as 80 °C. Due to the kinetic energy induced by this temperature the weak hydrogen bonds and hydrophobic interactions of common proteins become degraded and therefore coalesce and precipitate. (wikipedia.org)