- embryonic stem
- Pluripotent stem cells such as human ES cells (embryonic stem cells) and iPS cells (induced pluripotent stem cells) have been expected to be used and applied in various fields including regenerative medicine and cell therapy because they have the ability to be differentiated into various cells or tissues such as nerve cells and cardiac muscle cells, i.e., pluripotency. (patentsencyclopedia.com)
- A purified preparation of primate embryonic stem cells is disclosed. (google.es)
- In a particularly advantageous embodiment, the cells of the preparation are human embryonic stem cells, have normal karyotypes, and continue to proliferate in an undifferentiated state after continuous culture for eleven months. (google.es)
- b) culturing the primate embryonic stem cells so that they differentiate into derivatives of endoderm. (google.es)
- wherein the primate
- 3. The method according to claim 1, wherein the primate pluripotent stem cells are human pluripotent stem cells. (patentsencyclopedia.com)
- vitro
- Induced pluripotent stem cell technology makes in vitro reprogramming of somatic cells from individuals with various genetic backgrounds possible. (ahajournals.org)
- Mouse ES cells are undifferentiated, pluripotent cells derived in vitro from preimplantation embryos (Evans, at al. (google.es)
- The generation of β-cells in vitro is an attractive option for cell therapy treatments for type 1 diabetes and also for the development of more accurate disease models. (eurekaselect.com)
- A number of studies have demonstrated that insulin-expressing cells can be generated by the in vitro differentiation of human pluripotent stem cells. (eurekaselect.com)
- endoderm
- The embryonic stem cell lines also retain the ability, throughout the culture, to form trophoblast and to differentiate into all tissues derived from all three embryonic germ layers (endoderm, mesoderm and ectoderm). (google.es)
- Mouse ES cells cultured in non-attaching conditions aggregate and differentiate into simple embryoid bodies, with an outer layer of endoderm and an inner core of primitive ectoderm. (google.es)
- differentiation
- From these induced pluripotent stem cells, target cells and tissues can be generated after certain differentiation processes. (ahajournals.org)
- Mouse ES cells injected into syngeneic mice form teratocarcinomas that exhibit disorganized differentiation, often with representatives of all three embryonic germ layers. (google.es)
- In many cases, this is a result of an incomplete understanding of the regulatory processes involved in the differentiation of human pluripotent stem cells. (eurekaselect.com)
- regenerative medicine
- These target cells/tissues are expected to be useful in regenerative medicine, disease modeling, drug screening, toxicology testing, and proof-of-concept studies in drug development. (ahajournals.org)
- Therefore
- Therefore, the number of publications concerning induced pluripotent stem cells has recently been increasing rapidly, demonstrating that this technology has begun to infiltrate many aspects of stem cell biology and medical applications. (ahajournals.org)
- Therefore, frequent cryopreservation cannot be performed for primate pluripotent stem cells. (patentsencyclopedia.com)
- Therefore, in culture of primate pluripotent stem cells such as human ES cells and iPS cells, methods for simply maintaining and preserving pluripotent stem cells without cell passage while maintaining the properties of pluripotent stem cells other than cryopreservation are required. (patentsencyclopedia.com)
- human
- In this review, we discuss the perspectives of induced pluripotent stem cell technology for modeling human diseases. (ahajournals.org)
- The maintenance medium for human pluripotent stem cells according to the present invention includes xylose as a saccharide. (patentsencyclopedia.com)
- 6. The method according to claim 1, wherein the maintenance medium is a culture medium for human iPS cells containing xylose in place of glucose. (patentsencyclopedia.com)
- 7. The method according to claim 6, wherein the basal medium of a culture medium for human iPS cells is a DMEM/F12 medium. (patentsencyclopedia.com)
- In order to grow human ES cells or human iPS cells while maintaining the undifferentiation status and the pluripotency, it is usually needed to culture these cells in coexistence with feeder cells, or to add substances for maintaining undifferentiation status, for example, ascorbic acid, basic fibroblast growth factor (bFGF), and transforming growth factor β-3 (TGF-β3), to a medium (refer to WO 2011/058558). (patentsencyclopedia.com)
- In addition, for human iPS cells, since it is known that the properties of the cells are changed when the passage number exceeds a certain number, it is preferable to make the passage number as small as possible. (patentsencyclopedia.com)
- However, primate, especially human, ES cells and iPS cells are known to be sensitive to freezing and thawing and to have a low viability after cryopreservation. (patentsencyclopedia.com)
- Meanwhile, for human ES cells and iPS cells, it is difficult to prepare cells of different origin to a desired cell concentration during the same period because the growth rate is different by established cell line. (patentsencyclopedia.com)
- For human ES cells and iPS cells, it is recommended to change a medium every day due to their fast metabolism, and the risk of contamination is high, placing a burden on researchers. (patentsencyclopedia.com)
- However
- However, in pluripotent stem cells of primates including humans, it is not easy to grow the cells while maintaining the cell-specific properties, i.e., undifferentiation status and pluripotency (multipotency). (patentsencyclopedia.com)
- However, the passage process in culture in coexistence with feeder cells is complicated, and many of substances for maintaining undifferentiation status are expensive. (patentsencyclopedia.com)
- present
- The present invention relates to a maintenance medium for primate pluripotent stem cells, comprising xylose. (patentsencyclopedia.com)
- In general, the field of the present invention is stem cell cultures. (google.es)
- Specifically, the field of the present invention is primate embryonic stem cell cultures. (google.es)
- Given that miRNAs have the potential to influence cell fate, we present in this short review the evidence that a further understanding of the role of miRNAs in pancreatic development and function may be important in the on-going quest to generate insulin-secreting cells from pluripotent stem cells. (eurekaselect.com)
- maintain
- By applying this technology, it is possible to produce pluripotent stem cells from biopsy samples of arbitrarily selected individuals with various genetic backgrounds and to subsequently maintain, expand, and stock these cells. (ahajournals.org)
- Mouse ES cells maintain an undifferentiated state through serial passages when cultured in the presence of fibroblast feeder layers in the presence of Leukemia Inhibitory Factor (LIF) (Williams, et al. (google.es)
- animal
- Chemistry: molecular biology and microbiology animal cell, per se (e.g., cell lines, etc. (patentsencyclopedia.com)
- line
- A method for isolating a primate embryonic stem cell line is also disclosed. (google.es)
- culture
- Pluripotent stem cells can be grown by culture after cell establishment. (patentsencyclopedia.com)
- different
- For example, a hematopoietic stem cell may give rise to any of the different types of terminally differentiated blood cells. (google.es)
- method
- Provided are a maintenance medium for primate pluripotent stem cells, and a method for preserving and a method for controlling proliferation of primate pluripotent stem cells using the medium. (patentsencyclopedia.com)
- 1. A method for controlling proliferation of primate pluripotent stem cells, which comprises inhibiting the proliferation of primate pluripotent stem cells using a medium comprising xylose as a saccharide, which is substantially free of glucose. (patentsencyclopedia.com)
- 2. The method according to claim 1, which further comprises promoting the proliferation of primate pluripotent stem cells using a medium comprising glucose. (patentsencyclopedia.com)