• Primers
  • Next, an aqueous phase is prepared with the pre-loaded beads, PCR mixture, forward and reverse primers, and the paired end-tag library. (wikipedia.org)
  • The purpose of bead capping is to attach a "capping" oligonucleotide to the 3' end of both unextended forward ePCR primers and the RDV segment of template DNA. (wikipedia.org)
  • cells
  • Gorczyca W, Traganos F, Jesionowska H, Darzynkiewicz Z. Presence of DNA strand breaks and increased sensitivity of DNA in situ to denaturation in abnormal human sperm cells. (wikipedia.org)
  • fluorescent
  • The cap that being use is an amino group that prevents fluorescent probes from ligating to these ends and at the same time, helping the subsequent coupling of template DNA to the aminosilanated flow cell coverslip. (wikipedia.org)
  • FISH probes are generally labelled with a variety of different fluorescent tags and can only be detected under a fluorescence microscope, whereas CISH probes are labelled with biotin or digoxigenin and can be detected using a bright-field microscope after other treatment steps have been applied. (wikipedia.org)
  • subsequently
  • Boyd subsequently founded the Hannibal Records label in 1980 (later absorbed into Rykodisc), which released albums by Richard Thompson and many recordings of world music, including Hungarian band Muzsikás. (wikipedia.org)
  • reperfusion
  • Debate concerning prevalence of reperfusion injury continues but no true experimental model is presently available to distinguish damage caused by restoration of flow to the perfusion bed of the infarct related artery compared to that present at the end of ischemia. (omicsonline.org)
  • molecule
  • The study of this process in the zebrafish lateral line allows rapid morphological analysis of hair cell death and may be used as an efficient end point for molecule screens aimed at preventing these effects. (zfin.org)
  • Polony sequencing is generally performed on paired-end tags library that each molecule of DNA template is of 135 bp in length with two 17-18 bp paired genomic tags separated and flanked by common sequences. (wikipedia.org)
  • year
  • 17 January - The Chinese government announces plans for the first prototype exascale supercomputer by the end of the year. (wikipedia.org)
  • increase
  • Lastly, amplify the 135 bp paired end-tag library molecules with PCR to increase the amount of library material and eliminate extraneous ligation products in a single step. (wikipedia.org)
  • library
  • The protocol of Polony sequencing can be broken into three main parts, which are the paired end-tag library construction, template amplification and DNA sequencing. (wikipedia.org)
  • The resulting 135 bp library molecules are size-selected and nick translated. (wikipedia.org)
  • main
  • The main objectives of the park are ex-situ propagation of critically endangered species to prevent their extinction, wildlife education and interpretation aimed at a wider public appreciation of wildlife, and wildlife research to promote wildlife conservation and management. (wikipedia.org)
  • music
  • Boyd returned to the United States at the end of 1970 to work as a music producer for Warner Bros. with special input into films, where he collaborated with Stanley Kubrick on the sound track release of A Clockwork Orange. (wikipedia.org)
  • Corner Stone Cues (CSC) is a label and music production house based in Los Angeles. (wikipedia.org)