• nucleic
  • The hybridization signals for each probe when a nucleic abnormality is detected. (wikipedia.org)
  • In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA, RNA or modified nucleic acids strand (i.e., probe) to localize a specific DNA or RNA sequence in a portion or section of tissue (in situ), or, if the tissue is small enough (e.g., plant seeds, Drosophila embryos), in the entire tissue (whole mount ISH), in cells, and in circulating tumor cells (CTCs). (wikipedia.org)
  • probe
  • The probe must be large enough to hybridize specifically with its target but not so large as to impede the hybridization process. (wikipedia.org)
  • However, in situ hybridization requires that many steps be taken with precise optimization for each tissue examined and for each probe used. (wikipedia.org)
  • For hybridization histochemistry, sample cells and tissues are usually treated to fix the target transcripts in place and to increase access of the probe. (wikipedia.org)
  • tissue
  • RNA ISH (RNA in situ hybridization) is used to measure and localize RNAs (mRNAs, lncRNAs, and miRNAs) within tissue sections, cells, whole mounts, and circulating tumor cells (CTCs). (wikipedia.org)
  • In addition, in-situ hybridization on tissue sections require that tissue slices be very thin, usually 3 µm to 7 µm in thickness. (wikipedia.org)
  • Common methods of preparing tissue sections for in-situ hybridization processing include cutting specimens with a cryostat or a Compresstome tissue slicer. (wikipedia.org)
  • The tissue samples must then be washed and heated several times to remove any paraffin before the hybridization step. (wikipedia.org)
  • different
  • Data from different experiments may be used to normalize the fluorescence intensity while plasmids with a known number of telomeric repeats can be used as standards to help relate telomere fluorescence and telomere length. (wikipedia.org)
  • contribute
  • Bacteria affiliated with the Betaproteobacteria in the shape of long rods or chains of rods were found to contribute most to in situ E1 degradation. (biomedsearch.com)