• Poly-histidine tags (of six or more consecutive H residues) are utilized for protein purification by binding to columns with nickel or cobalt, with micromolar affinity. (wikipedia.org)
  • Previous studies using rat renal brush-border membrane vesicles indicated that cysteine and histidine residues played critical roles in H + /organic cation antiport activity. (aspetjournals.org)
  • In the present study, essential histidine and cysteine residues of MATE1 family were elucidated. (aspetjournals.org)
  • When 7 histidine and 12 cysteine residues of rat (r)MATE1 conserved among species were mutated, substitution of His-385, Cys-62, and Cys-126 led to a significant loss of tetraethylammonium (TEA) transport activity. (aspetjournals.org)
  • These results suggest that histidine and cysteine residues are required for MATE1 to function and that cysteine residues may serve as substrate-recognition sites. (aspetjournals.org)
  • Histidine residues have been suggested as triggers due to their approximate textbook pK(a) value of 6.1 for their side chains. (uni-koeln.de)
  • We present here an extensive theoretical and experimental study of the 14 histidine residues present in canine HSP47, where we have mutated all histidine residues in the collagen binding interface and additionally all of those that were predicted to undergo a significant change in protonation state between pH 7 and 6. (uni-koeln.de)
  • Sekler, I , Kobayashi, S & Kopito, RR 1996, ' A cluster of cytoplasmic histidine residues specifies pH dependence of the AE2 plasma membrane anion exchanger ', Cell , vol. 86, no. 6, pp. 929-935. (bgu.ac.il)
  • Three have histidine residues and the fourth is OPL. (cdc.gov)
  • Histidine (symbol His or H) is an essential amino acid that is used in the biosynthesis of proteins. (wikipedia.org)
  • Humans and other animals must ingest histidine or histidine-containing proteins. (wikipedia.org)
  • The chromatography medium provides very high binding capacity for histidine-tagged proteins and shows negligible leakage of Ni 2+ ions. (sigmaaldrich.com)
  • Ni Sepharose High Performance precharged with Ni2+ for high-performance purification of histidine-tagged proteins. (sigmaaldrich.com)
  • To prevent the binding of host cell proteins with exposed histidines, it is essential to include imidazole at a low concentration in the sample and binding buffer ( Optimizing purification of histidine-tagged proteins ). (sigmaaldrich.com)
  • Histidine-containing phosphotransfer proteins (HPts) take part in hormone signal transduction in higher plants. (rcsb.org)
  • HPts function in histidine-aspartate phosphorelays in which they mediate the signal from sensory kinases (usually membrane proteins) to RRs in the nucleus. (rcsb.org)
  • GE Healthcare has announced the availability of its single-use spin column designed for purification of histidine-tagged proteins using a standard microcentrifuge. (outsourcing-pharma.com)
  • In this study, we have explored several humanized histidine-rich peptides in tumor-targeted modular proteins, which can specifically bind and be internalized by the target cells through the tumoral marker CXCR4. (upc.edu)
  • We were particularly interested in exploring how protein purification, self-assembling and endosomal escape perform in proteins containing the variant histidine-rich tags. (upc.edu)
  • Histidine kinase 2 is a sensor of sodium ion concentration and redox potential, regulating transcription of genes for light-harvesting and reaction center proteins of photosynthesis in cyanobacteria and chloroplasts of algae and plants. (biorxiv.org)
  • Our hypothesis is that this effect is of electrostatic origin and that the observed behavior is due to proton charge fluctuations of the histidines, promoting attractive electrostatic interactions between the positively charged proteins and the anionic surfaces, with concomitant counterion release. (lu.se)
  • Histidine, one of several so-called essential amino acids for humans, was first isolated in 1896 from various proteins. (maxmedchem.com)
  • Histidine and its side effects, Proteins and enzymes are created in the body using amino acids. (healthylifehuman.com)
  • Ni Sepharose provides a high binding capacity-up to 750 µg of pure histidine-tagged protein per column-and compatibility with a variety of commonly used additives and denaturing and reducing agents. (outsourcing-pharma.com)
  • Location of study sites at Ghindae and Massawa Hospitals, Eritrea, for analysis of a major threat to malaria control programs by Plasmodium falciparum lacking histidine-rich protein 2. (cdc.gov)
  • Poly-histidine peptides such as H6 (HHHHHH) are used in protein biotechnologies as purification tags, pro- tein-assembling agents and endosomal-escape entities. (upc.edu)
  • The sensor component is a protein that becomes covalently modified by a phosphate group on a histidine side chain. (biorxiv.org)
  • Histatin 5 is a histidine-rich, intrinsically disordered, multifunctional saliva protein known to act as a first line of defense against oral candidiasis caused by Candida albicans. (lu.se)
  • It has been hypothesized that conserved histidines in the class II fusion protein E of these viruses function as molecular switches and, by their protonation, control the fusion process. (rupress.org)
  • Histidine amino acid , a protein amino acid obtained through hydrolysis. (maxmedchem.com)
  • The protein haemoglobin depends on histidine to stay in its normal range of concentrations. (healthylifehuman.com)
  • mAb 18.2, which recognises the Knob Associated Histidine Rich protein (KAHRP) is now available. (malariaresearch.eu)
  • ZIP4 is a member of the Zrt-/Irt-like protein (ZIP) family, and it is a histidine-rich transmembrane protein that is specifically expressed on the apical side of enterocytes in the small intestine, as well as in the kidney, and is responsible for zinc absorption. (medscape.com)
  • l-Histidine is an essential amino acid that is not synthesized de novo in humans. (wikipedia.org)
  • L-Histidine is an essential amino acid. (sigmaaldrich.com)
  • Here we are investigating the role of the histidines in more detail by defining a library of variants of the peptide, where the former have been replaced by the pH-insensitive amino acid glutamine. (lu.se)
  • Histidine amino acid is an essential amino acid that is required by the body primarily during childhood growth. (maxmedchem.com)
  • Because it increases calcium absorption and lowers amino acid amino acids histidine levels, it can control diarrhea. (maxmedchem.com)
  • An important amino acid is histidine. (healthylifehuman.com)
  • Histidine is a necessary amino acid, however adults do not seem to need it. (healthylifehuman.com)
  • Histidine is an amino acid used to treat metabolic syndrome, diarrhea caused by cholera infection, rheumatoid arthritis, allergic diseases, ulcers, and anemia caused by kidney failure or kidney dialysis. (drbillspetnutrition.ca)
  • The essential amino acid histidine is needed for the growth and repair of tissue, particularly for the maintenance of myelin sheaths-sleeves of fatty tissue that protect nerve cells, ensuring that they're able to send and receive messages. (mindbodygreen.com)
  • Histidine is thought to be a human essential amino acid, mainly children. (nmn-powder.com)
  • L-histidine is a semi-essential amino acid that is particularly important for infants and animals. (nmn-powder.com)
  • 5. Histidine is a semi-essential amino acid that is particularly important for infants and animals. (nmn-powder.com)
  • The imidazole sidechain of the histidine residue commonly serves as a ligand in metalloproteins. (wikipedia.org)
  • The transport of TEA via rMATE1 was inhibited by the sulfhydryl reagent p -chloromercuribenzenesulfonate (PCMBS) and the histidine residue modifier diethyl pyrocarbonate (DEPC) in a concentration-dependent manner. (aspetjournals.org)
  • These biopolymers have a histidine residue, which is a potent quencher of tryptophan fluorescence, positioned four amino acids away from tryptophan. (montana.edu)
  • Precursor of histamine by action of histidine decarboxylase. (sigmaaldrich.com)
  • WO9830593A2 ] The present invention provides a purified antibody which specifically reacts with human histidine decarboxylase (HDC), as well as immunogenic compositions comprising HDC peptides. (epo.org)
  • The disease dietary histidine is associated with low blood pressure. (healthylifehuman.com)
  • Here, we find that dietary histidine plays a key role in the response to an LP diet in male C57BL/6J mice. (niagads.org)
  • Finally, we demonstrate that variation in dietary histidine levels helps to explain body mass index differences in humans. (niagads.org)
  • Overall, our findings demonstrate that dietary histidine is a key regulator of weight and body composition in male mice and in humans, and suggest that reducing dietary histidine may be a translatable option for the treatment of obesity. (niagads.org)
  • The objectives of the proposed Transnational Network are to bring together scientists from academia and industry working in relevant multidisciplinary fields to develop integrated strategies to overcome the permeability barrier of Gram-negative cell envelopes and further develop newly discovered histidine kinase inhibitors as anti-virulence therapies. (wur.nl)
  • Histidine kinase inhibitors are being developed as a new class of antimicrobial drugs. (uky.edu)
  • We recently demonstrated the activity of a class of histidine kinase inhibitors against a mammalian enzyme, elongation factor-2 kinase (eEF-2K), and the effect of these compounds on cancer cell viability (Arora et al. (uky.edu)
  • These observations indicate that certain histidine kinase inhibitors are substrates for P-gp and hence an important consideration in development of these agents as potential antimicrobial and anticancer agents. (uky.edu)
  • The body uses histidine to make histamine. (healthylifehuman.com)
  • Investigation on beneficial role of l-carnosine in neuroprotective mechanism of ischemic postconditioning in mice: possible role of histidine histamine pathway. (bvsalud.org)
  • The present study was undertaken to investigate the possible role of histidine - histamine pathway in the neuroprotective effects produced by L-carnosine hand in hand with ischemic postconditioning in the animal model of cerebral ischemia . (bvsalud.org)
  • It is concluded that L-carnosine exerts neuroprotective effect via involvement of histidine - histamine pathway since the beneficial effects of L-carnosine were abolished by the H2-blocker. (bvsalud.org)
  • Using the mutational analysis of recombinant subviral particles of tick-borne encephalitis virus, we provide direct experimental evidence that the initiation of fusion is crucially dependent on the protonation of one of the conserved histidines (His323) at the interface between domains I and III of E, leading to the dissolution of domain interactions and to the exposure of the fusion peptide. (rupress.org)
  • Protonation of the proximal histidine ligand in heme peroxidases. (lu.se)
  • Natural poly-histidine peptides, found in the venom of the viper Atheris squamigera have been shown to bind Zn(2+), Ni(2+) and Cu(2+) and affect the function of venom metalloproteases. (wikipedia.org)
  • The biosynthesis of histidine has been widely studied in prokaryotes such as E. coli. (wikipedia.org)
  • A decrease in the number of histidine from the original seven to zero decreases the ability of the peptide to penetrate the bilayer, and the peptide is then also found residing within the bilayer. (lu.se)
  • At large distances this solvation decreases the ability of histidine to accept an electron from tryptophan. (montana.edu)
  • We have previously identified a panel of inhibitors targeting bacterial histidine kinases in bacteria that inhibit targets involved in the regulation of virulence and stress response pathways in bacterial pathogens (Velikova et al. (wur.nl)
  • The activities of this Transnational Network are aimed at aligning international research activities and combining expertise to devise the most efficient strategy to further develop new anti-infective drugs targeted to histidine kinases. (wur.nl)
  • Two-component signal transduction systems (TCSs) consist of sensor histidine kinases and response regulators. (biorxiv.org)
  • Dealing in Active Pharmaceutical Ingredients : Acesulfame Potassium USP, Betacyclo Dextrin USP, L Histidine. (needsinfo.com)
  • Products Offered : Amino Acids, Nutraceuticals and Other Chemicals, Active Pharmaceutical Ingredients L Histidine. (needsinfo.com)
  • In carbonic anhydrases, a histidine proton shuttle is utilized to rapidly shuttle protons away from a zinc-bound water molecule to quickly regenerate the active form of the enzyme. (wikipedia.org)
  • Food supplements zinc , histidine, vitamin C. The recommended daily intake must not be exceeded. (eliveragroup.com)
  • Dietary supplements zinc , histidine, vitamin C are no substitute for a balanced, varied diet and a healthy lifestyle. (eliveragroup.com)
  • Histidine is involved with the regulation and utilisation of several essential trace minerals in the body including zinc, copper, manganese and iron. (approvedvitamins.com)
  • Inactivation of plasma alpha1-proteinase inhibitor by acrolein: adduct formation with lysine and histidine. (cdc.gov)
  • Histidine is one of the essential raw materials for the synthesis of some pharmaceutical intermediates. (nmn-powder.com)
  • In catalytic triads, the basic nitrogen of histidine abstracts a proton from serine, threonine, or cysteine to activate it as a nucleophile. (wikipedia.org)
  • In microorganisms histidine is orchestrated from the sugar ribose and the nucleotide adenosine triphosphate. (maxmedchem.com)
  • Histidine is synthesized from phosphoribosyl pyrophosphate (PRPP), which is made from ribose-5-phosphate by ribose-phosphate diphosphokinase in the pentose phosphate pathway. (wikipedia.org)
  • The conjugate acid (protonated form) of the imidazole side chain in histidine has a pKa of approximately 6.0. (wikipedia.org)
  • The imidazole/imidazolium ring of histidine is aromatic at all pH values. (wikipedia.org)
  • L-histidine and a-amino-b-[4-imidazole]-propionic acid are other names for histidine. (healthylifehuman.com)
  • Experiments assumed that when folding occurs the fluorescence of tryptophan will be quenched by histidine due to the formation of an alpha helix. (montana.edu)
  • Simulations for HP-35 and WH5 indicate that the ability of histidine to quench the fluorescence of tryptophan is surprisingly controlled by the energy gap dependence on the distance that separates them. (montana.edu)
  • Different tryptophan/histidine rotamers control this distance. (montana.edu)
  • Even when HP-35 is completely folded much of the time histidine does not quench tryptophan fluorescence contrary to the idea that histidine is only close when HP-35 is folded. (montana.edu)
  • We focused this work on a representative compound (NH125) because we found it to be most potent against both histidine kinase and eEF-2K among the series. (uky.edu)
  • Histidine kinase 2 (Hik2) is a sensor histidine kinase found in all known cyanobacteria and as chloroplast sensor kinase in eukaryotic algae and plants. (biorxiv.org)
  • The data in this paper identify a histidine-rich sequence within the cytoplasmic domain of the nonerythroid anion exchanger, AE2, that serves as an intracellular pH 'sensor' that modulates anion exchange activity within the physiological range of cytoplasmic pH. (bgu.ac.il)
  • Dietary levels of histidine are positively associated with body mass index in humans. (niagads.org)
  • In helices E and F of haemoglobin, histidine influences binding of dioxygen as well as carbon monoxide. (wikipedia.org)
  • The liver produces histidine in small quantities. (healthylifehuman.com)
  • It is discovered at higher levels when there is an allergic response, even if histidine produces it as a byproduct. (healthylifehuman.com)
  • For allergic diseases such as asthma, L-histidine also has effect. (nmn-powder.com)
  • L-Carnosine is a dipeptide molecule and non-enzymatic free radical scavenger and a natural antioxidan, made up of the amino acids beta-alanine and histidine. (marknature.com)
  • The energy gap dependence on this distance arises from water solvation around histidine. (montana.edu)
  • Trade & Import - Export of Chemicals, L-Arginine Base, L-Arginine Base HCl, L Histidine. (needsinfo.com)
  • Surprisingly, the effects of histidine restriction do not require the energy balance hormone Fgf21. (niagads.org)
  • Specifically reducing dietary levels of histidine by 67% reduces the weight gain of young, lean male mice, reducing both adipose and lean mass without altering glucose metabolism, and rapidly reverses diet-induced obesity and hepatic steatosis in diet-induced obese male mice, increasing insulin sensitivity. (niagads.org)
  • However, it was shown to affect the penetration depth of the peptide into the bilayer, where all variants except the one with zero histidines were found below the bilayer. (lu.se)
  • Conserved histidines located outside this critical interface were found to be completely dispensable for triggering fusion. (rupress.org)
  • Reduced histidine promotes leanness and increased energy expenditure in male mice. (niagads.org)
  • Reduced histidine does not extend the lifespan of mice when begun in midlife. (niagads.org)
  • Different food items that incorporate histidine are given as meat, fish, and dairy items. (maxmedchem.com)
  • L-histidine may help joints work well, especially when gamma globulin is present in the synovial fluid. (maxmedchem.com)
  • In addition, histidine is also present in green vegetables, but less. (nmn-powder.com)
  • Here we are investigating the role of the histidines in more detail by defining a library of variants of the peptide, where. (lu.se)
  • The body uses histidine to create certain hormones and metabolites that influence kidney function, nerve conduction, stomach secretions, and the immune system. (healthylifehuman.com)