High throughput screening assays. Medical search. Frequent questions

By coupling efficient chemical synthesis routes to multiple upfront in parallel phenotypic screens, we identify that KBU2046 inhibits cell motility and cell invasion in vitro. (nature.com)
Here we present an in-vitro method for screening RNA protein interactions that circumvents these problems. (nih.gov)
The presence of endotoxin can significantly impact both in vitro and in vivo experiments, causing non-specific cellular responses and false readings in cell-based assays. (abcam.com)
Molecular Devices Corporation announced the launch of its QBT Fatty Acid Uptake Assay Kit, the first assay kit that addresses the need for a single-step, homogenous in vitro assay for fatty acid uptake for use in high- throughput screening and metabolic disease research. (news-medical.net)
These experiments establish the feasibility of utilizing an in vitro cell based uptake/clearance assay coupled with pharmacokinetic modeling as a novel chemical screening strategy to identify ideal chemical candidates for saliva biomonitoring. (cdc.gov)
The most active compounds were further screened in vitro against the chloroquine resistant strain P. falciparum, (RKL9) and showed IC50 value range of 0.0198-0.096 µM, compared to IC50 value of 0.19420 µM for chloroquine sulphate. (bvsalud.org)

Here we describe the results of a programme that aimed to rapidly discover lead compounds for clinical use, by combining structure-assisted drug design, virtual drug screening and high-throughput screening. (nature.com)
Through a combination of structure-based virtual and high-throughput screening, we assayed more than 10,000 compounds-including approved drugs, drug candidates in clinical trials and other pharmacologically active compounds-as inhibitors of M pro . (nature.com)
One of these compounds (ebselen) also exhibited promising antiviral activity in cell-based assays. (nature.com)
To facilitate the rapid discovery of antiviral compounds with clinical potential, we developed a strategy that combines structure-assisted drug design, virtual drug screening and high-throughput screening to repurpose existing drugs to target SARS-CoV-2 M pro . (nature.com)
Molecules identified in the screen can serve as potential lead compounds for the development of anti-angiogenic and anti-anthrax therapies. (johnshopkins.edu)
Preliminary screens conducted with a library of known bioactive compounds identified tannic acid and cisplatin as inhibitors of the PA-CMG2 interaction. (johnshopkins.edu)
We developed Redistribution cell-based assays for use in both primary screening and lead optimization of compounds targeting GPCRs. (genengnews.com)
The Chemical Biology Core Facility (CBCF) offers the infrastructure and expertise for assay development, small-molecule screening and use of medicinal chemistry to optimise compounds against novel targets for 'biotool' or early drug development. (embl.org)
This is partly due to the poorly representative models used for screening in the early stages of drug discovery, leading to a high failure rate of compounds later in the drug development pipeline. (moleculardevices.com)
The challenge - accurate selection of compounds from early screens, for further development and clinical testing. (moleculardevices.com)
The same principle can be used to identify new therapeutics that are working effectively to kill cancer cells as part of a screen from a library of compounds. (moleculardevices.com)
How much to compress a library can be influenced by the quality of the library, purity, devoid of bad actors and PAINS compounds, the throughput of the ASMS approach and the target of interest. (ddw-online.com)
To accelerate screens, most users of ALIS utilise highly compressed libraries with hundreds or thousands of compounds per reaction. (ddw-online.com)
The K D estimates for a set of compounds selected from mono- and di-saccharides with affinities across a 550-fold range, correlated well between FP and SPR assay formats for both human and mouse galectin-3. (lu.se)
Together with an industrial partner Cellevate, an innovative biotech company based in Lund, the researchers developed a new method for medium-high throughput drug screening of human airway cells which allows several hundred compounds to be evaluated on cells from individual patients. (lu.se)

High-Throughput Screening Assays in Toxicology. (wikipedia.org)
9 ) with the determination of the RC50 value being the marker reactivity of test chemicals as end point toxicology assays have of the potency of a chemical. (cdc.gov)

To characterize the enzymatic activity of SARS-CoV-2 M pro and to carry out high-throughput screening of inhibitors, we developed a fluorescence resonance energy transfer assay. (nature.com)
Here we describe development and testing of an effective homogeneous steady-state fluorescence resonance energy transfer (FRET) high throughput screening assay designed to identify molecules that inhibit binding of PA to CMG2. (johnshopkins.edu)
Since this assay uses a fluorescence-based readout rather than a radioactivity-based one, it eliminates the safety risks associated with conventional radiolabel assays, in addition to being faster and easier to perform. (news-medical.net)
Historically, the screening and triaging of small molecule glycomimetics that bind to the galectin-3 CRD has been completed in fluorescence polarisation (FP) assays to determine K D values. (lu.se)

The assay to screen for inhibitors of this protein-protein interaction is sensitive and robust, with observed Z' values as high as 0.92. (johnshopkins.edu)
This work represents the first reported high throughput screening assay targeting CMG2 to identify possible inhibitors of both angiogenesis and anthrax intoxication. (johnshopkins.edu)
The purified recombinant CHIKV nsP1 has been used for the development of a rapid and sensitive non-radioactive enzyme linked immunosorbent assay (ELISA) to identify the inhibitors of CHIKV nsP1. (tenoeverlab.com)

High-throughput nuclear magnetic resonance metabolomics and biochemical assays were used to quantify 87 circulating metabolic measures in seven cohorts from Finland and the UK, comprising altogether 18 288 individuals (mean age 26 years, range 15-75). (nih.gov)

The NIH Molecular Libraries Roadmap Initiative wishes to solicit HTS assay applications from the scientific community from investigators who have the interest and capability to work with the Molecular Libraries Probe Production Centers Network (MLPCN) in support of chemical probe development. (nih.gov)
Processes that drive the development of increased cell motility represent high-value therapeutic targets. (nature.com)
To facilitate development of henipavirus antivirals, a high-throughput screening (HTS) platform was developed based on a well-characterized recombinant version of the nonpathogenic Henipavirus, Cedar virus (rCedV). (utmb.edu)
We experimented with cells division-arrested with low level mitomycin C treatment and demonstrated that they perform with better consistency than non-division arrested counterparts in high-content screening imaging assays, says Zhong Zhong, vp of development at CMT. (genengnews.com)
Sentigen Biosciences developed Tango assays for GPCR profiling of targets of relevance to pharmaceutical discovery and development. (genengnews.com)
For development of Redistribution assays, BioImage (www.bioimage.com) uses Aequorea victoria GFP and other fluorescent proteins fused to translocating targets of interest. (genengnews.com)
The development of fatty acid uptake regulators as potential drugs is dependent upon having a rapid assay that can be used in high-throughput screening. (news-medical.net)
Williams-Clark continued, "Prior to the development of this assay, it was extremely difficult for drug discovery researchers to study fatty acid uptake processes and identify new regulators. (news-medical.net)
Minimize assay development time and eliminate the need for cell culture. (discoverx.com)
Non-invasive saliva human biomonitoring: development of a transwell assay to support exposure science assessment and health impacts. (cdc.gov)
Gheit T, Landi S, Gemignani F, Snijders PJ, Vaccarella S, Franceschi S, Canzian F, Tommasino M. (2006) Development of a sensitive and specific assay combining multiplex PCR and DNA microarray primer extension to detect high-risk mucosal human papillomavirus types. (who.int)

Redistribution assays are quantitative assays that can be run in standard microtiter plates. (genengnews.com)
This reactivity of chemicals to cutaneous the peptide reactivity assay ( 10 ), quantitative LC-MS was proteins is the basis for most, if not all, current nonanimal- exploited by Natsch et al. (cdc.gov)

Our Surface Plasmon Resonance (SPR) Biacore T100 biosensor instrument is a high-throughput equipment that allows the study of protein-protein and protein-ligand kinetics and thermodynamics. (utmb.edu)

CMT also offers a cell-based assay technology based on Sentigen Biosciences (www.sentigen.com) Tango Assay System. (genengnews.com)
Redistribution assays are independent of the imaging platform and have been shown to give consistent results on a number of instruments on the market, including: IN Cell 3000 (GE Healthcare), Opera (Evotec Technologies), ArrayScan (Cellomics), Pathway HT (BD Biosciences), Discovery1 (Molecular Devices), and Icyte (CompuCyte). (genengnews.com)

Building upon the foundation of basics discussed in the previous edition, the Second edition provides a more in-depth look at the latest methods and technologies of advanced drug screening, an essential function of drug discovery. (chipsbooks.com)
Rapid methods of measuring the effects of an agent in a biological or chemical assay. (bvsalud.org)

The colony formation assay is the gold-standard technique to assess cell viability after treatment with cytotoxic reagents, ionizing radiation, and cytotoxic combinatorial treatments. (ox.ac.uk)
High-content imaging can also be used to determine cell viability by counting the number of live or dead cells (labelled with dyes such as Calcein AM, a common live cell marker) in a drug-treated sample in comparison to an untreated control (and possibly organoids derived from non-cancer-tissue). (moleculardevices.com)

eXpress Assay kits developed by Eurofins DiscoverX ® are ready-to-use (RTU) cell-based assay kits for functional mechanism of action (MOA) or binding assay for drug discovery target validation, screening and hit ID, and lead optimization applications. (discoverx.com)

Our results demonstrate the efficacy of our screening strategy, which can lead to the rapid discovery of drug leads with clinical potential in response to new infectious diseases for which no specific drugs or vaccines are available. (nature.com)

Additionally, using the developed ELISA nsP1 assay, the inhibitory effects of sinefungin, aurintricarboxylic acid (ATA) and ribavirin were determined and the IC 50 values were estimated to be 2.69 μM, 5.72 μM and 1.18 mM, respectively. (tenoeverlab.com)
Cell based inhibitory assays of these drugs against M. tb (H37Ra) culture indicates their potential to interfere with pathogen growth and replication. (bvsalud.org)

Virtually all these approaches (e.g. flow cytometry, high content imaging) offer only end point assays, so numerous experiments are needed to track internalization over time. (news-medical.net)

As cell-based assays are used more commonly in robotic high-throughput compound screening, cells themselves have become critical reagents. (genengnews.com)

www.cmt-inc.net), makes it possible to use frozen assay-ready cells for screening as if they were ordinary assay reagents like enzymes or membrane preparations. (genengnews.com)

Advanced high-throughput screening technologies require standardized, consistent cell culture as reagents to generate reliable results. (genengnews.com)
Thus, it has become essential to produce cell reagents with high consistency and quality. (genengnews.com)
These ready-to-use reagents can be thawed, plated, and used in screening with improved consistency and convenience. (genengnews.com)
Each assay kit includes RTU cryopreserved cells, optimized assay reagents, plates, and a detailed assay protocol to ensure robust and reliable performance every time. (discoverx.com)

Conducting molecular and cell biology assays in both human and mouse cell lines. (academicjobsonline.org)

The results from this prevali- the field of cancer biomarkers (11-13), and that approach was dation study showed that patients could be classified into high- adopted here to define predictive serum biomarkers associated versus low-risk groups for developing metastatic breast cancer with tumor relapse in breast cancer patients. (lu.se)

This initiative is one of the integrated components of the NIH Molecular Libraries Roadmap initiative that offers biomedical researchers access to large-scale automated high throughput screening (HTS) centers in the MLPCN, diverse compound libraries in the Small Molecule Repository (MLSMR) and information on biological activities of small molecules in the PubChem BioAssay public database. (nih.gov)
This protocol describes a high-throughput automated and high-content imaging approach to screen siRNA molecular libraries in HeLa cervical cancer cells in 96-well format. (ox.ac.uk)
2018). This demonstrates the proof of principle that organoids can be used for screening "libraries" of potential therapeutics. (moleculardevices.com)

Comprehensive molecular, cellular and systemic-level assays all support a high level of selectivity. (nature.com)
We do so by demonstrating selectivity across comprehensive molecular, cellular, and systemic assays. (nature.com)
Coherent metabolic signatures between lower birthweight and higher adult adiposity suggest that shared molecular pathways may potentially underpin the metabolic deviations. (nih.gov)
Susan Williams-Clark, Director of Marketing at Molecular Devices stated, "Working together with researchers at the Palo Alto Medical Foundation's Research Division, we have applied our proprietary quench technology to develop the first single-step homogenous assay for fatty acid uptake. (news-medical.net)
Similar to Molecular Devices' FLIPR Assay Kits, the QBT Fatty Acid Uptake Assay eliminates the need for cell washing steps, resulting in a faster, more streamlined route to drug discovery. (news-medical.net)
Molecular dynamics simulations confirmed a stable and high potential binding of the most active candidates against leishmanial PTR1. (bvsalud.org)

3D organoids are a relatively new technology and assays to make full use of them are still being invented and developed. (moleculardevices.com)

Develop, perform and analyze immuno-cell based assays data to determine the productivity of the platform process as well as to expedite project timeline. (biospace.com)
Screening using cell-based assays has been performed for many years, but recent technologies allow screening of cells that are alive and changing. (genengnews.com)
Cell-based assays are becoming increasingly popular for small molecule discovery in high-throughput screening. (genengnews.com)
For cell-based screening, it is possible to uncouple the cell production process from the screening process. (genengnews.com)
The idea of a robust, cell-based assay is no longer an oxymoron. (genengnews.com)
This expanding panel of cell-based receptor assays, which currently includes 54 targets, is being offered by CMT for custom compound profiling to evaluate compound selectivity over a wide range of likely GPCR targets. (genengnews.com)

We screen approximately 4000 alternatively spliced exons and the entire Hepatitis C genome for binding of ASF/SF2, the only splicing factor demonstrated to function as an oncogene. (nih.gov)

Principle of antibody Internalization assay using Incucyte ® FabFluor labeling reagent. (news-medical.net)
Each varies in the experimental design, throughput, reagent requirements and mass spectrometer instrument. (ddw-online.com)

By eliminating lengthy, time-consuming, and expensive cell culture steps, eXpress assay kits enable you to screen your small molecule or biologic therapeutics and generate accurate, precise, and reproducible functional data in just a few hours. (discoverx.com)
These similarities make them suitable as subjects for testing such as behavioural assays and functional brain imaging. (kcl.ac.uk)

The Swarm can screen ~10,000 wells/day in 384-well format, probing complex pharmacological interactions via a wide array of stimulation protocols. (frontiersin.org)

Using reverse genetics, a rCedV encoding firefly luciferase (rCedV-Luc) was rescued and its utility evaluated for high-throughput antiviral compound screening. (utmb.edu)

Redistribution assays can be multiplexed by either expressing two targets in the same cell line labeled with different colors, or by mixing different assay cell lines directly in the microtiter plate well. (genengnews.com)
Optogenetic assays provide a flexible, scalable, and information rich approach to probe compound effects for ion channel drug targets in both heterologous expression systems and associated disease relevant cell types. (frontiersin.org)
We believe that the QBT Fatty Acid Uptake Assay Kit will enable high-throughput screening of these drug targets and enhance fatty acid metabolism research. (news-medical.net)

Both the labeling method and assay workflow are ideal for assessing internalization rates of many antibodies (10-100s) in miniaturized (96/384 well) plate formats. (news-medical.net)

Homogeneous Cell- and Bead-Based Assays for High Throughput Screening Using Fluorometric Microvolume Assay Technology. (thermofisher.com)
The homogeneous assay kit format allows you to detect agonists, antagonists, and allosteric modulators. (discoverx.com)

By increasing the scale and scope of organoid experiments, this automated assay complements other patient-derived models and will expedite preclinical testing of new treatments for prostate cancer. (edu.au)
Zebrafish larvae are an excellent preclinical model that allow high-throughput approaches in line with the 3Rs and we are excited to embark on this project. (kcl.ac.uk)

2022), using the US EPA Toxicity Forecaster (ToxCast) assay data and the curated mapping of key characteristics to assays available at the time of the evaluations performed for IARC Monographs Volume 131. (who.int)

The QBT Fatty Acid Uptake Assay removes these limitations by providing a rapid, reproducible, inexpensive method for laboratory screening. (news-medical.net)

These assays usually require labeling each antibody with a fluorescent tag, and in nearly all of these cases the labeled antibody must then be split from the free label via a wash step or column. (news-medical.net)
In the present study, chikungunya virus (CHIKV) nsP1 MTase and GT reactions were confirmed by an indirect non-radioactive colorimetric assay and western blot assay using an antibody specific for the m 7 G cap, respectively. (tenoeverlab.com)
The developed ELISA nsP1 assay measures this m 7 GMP-nsP1 complex by utilizing anti-m 7 G cap monoclonal antibody. (tenoeverlab.com)

Surface plasmon resonance (SPR) has not been widely used for compound screening and in this study it was used to compare human and mouse galectin-3 affinity measures between FP and SPR, as well as investigate compound kinetics. (lu.se)

SPR has been shown to be a viable alternative to FP for early drug discovery screening and determining K D values. (lu.se)

We have a set of different sized cuvettes to allow optimization of operating conditions, from high volume 1 cm cells to low volume "sandwich cells" especially suitable for "non-transparent" buffers. (utmb.edu)

Neural Progenitor Cells Derived from XCL-1 DCXp-GFP are a neuronal lineage reporter cell line that can be used in neuronal differentiation and drug screening. (atcc.org)
Cells can be produced en masse, treated to become irreversibly division-arrested, and cryopreserved. (genengnews.com)
To address this challenge, we developed the Swarm TM , a custom designed optical instrument for highly parallelized, multicolor measurements in excitable cells, simultaneously recording changes in voltage and calcium activities at high temporal resolution under optical stimulation. (frontiersin.org)
One frequently used technique is a bioluminescent-based assay which detects the presence of metabolically active cells by measuring ATP levels. (moleculardevices.com)
The number of cells that are still alive in the untreated sample will be considerably more than in the drug-treated cells, especially where high concentrations of the therapy are used. (moleculardevices.com)
Schmitt M, Dondog B, Waterboer T, Pawlita M, Tommasino M, Gheit T. (2010) Abundance of multiple high-risk human papillomavirus (HPV) infections found in cervical cells analyzed by use of an ultrasensitive HPV genotyping assay. (who.int)
This model that has been developed with our collaborators will fill an important gap for current and future drug screening efforts for the upper or lower airways using human airway cells", says Sinem Tas, associate researcher. (lu.se)

The rCedV-Luc platform was optimized as an HTS assay that demonstrated high sensitivity with robust Z′ scores, excellent signal-to-background ratios and coefficients of variation. (utmb.edu)
In addition, it can also provide early kinetic characterisation of small molecule galectin-3 glycomimetics with robust k on and k off values generated in a high throughput manner. (lu.se)

As a demonstration of the versatility of the Swarm, we also developed an assay measuring cardiac action potential and calcium waveform properties simultaneously under paced conditions using human induced pluripotent stem (iPS) cell-derived cardiomyocytes as an additional counter screen for cardiac toxicity. (frontiersin.org)

We illustrate how this method could be employed to screen disease alleles with the set of small molecules that have been shown to alter splicing in search for therapies for splicing diseases. (nih.gov)

Despite the potential utility and growing adoption of optogenetics, there remains a critical need for compatible platform technologies with the speed, sensitivity, and throughput to enable their application to broader drug screening applications. (frontiersin.org)

Isothermal titration calorimetric analyses showed that the drugs can bind with high affinity to target proteins and interfere with known protein-protein interaction of MoxR1 and RipA. (bvsalud.org)
The reduction in affinity observed between human to mouse galectin-3 was also comparable between assay formats. (lu.se)

From the pre-mRNA sequences returned in this screen we discovered physiologically relevant ASF recognition element motifs. (nih.gov)

Through its funding of the MLPCN, NIH will support the costs of assay automation, screening and optimization chemistry. (nih.gov)
High Throughput Screening (HTS) allows a researcher to quickly conduct thousands of tests against a biological target through the use of automation (robotics, liquid handling devices, sensitive detectors), data processing and control software. (embl.org)
The assay usually involves some form of automation or a way to conduct multiple assays at the same time using sample arrays. (bvsalud.org)

Historically, binding assays have relied on displacement formats utilising fluorescent and radioactive probes 1,2 that required synthesising specific probes, thereby limiting the approach to identifying competitive molecules. (ddw-online.com)
Unlike many indirect binding assays, a major advantage of detecting the mass ID of a binding molecule is that multiple molecules can be tested simultaneously. (ddw-online.com)

These formats however, offer indirect hit finding strategies and therefore, are often associated with high false positive rates and progress delays. (ddw-online.com)

This simple, rapid, and inexpensive absorbance-based method has great potential for use as a preliminary screening tool for skin allergens. (cdc.gov)

Executing DNA repair assays and data analysis. (academicjobsonline.org)

High-throughput screening has been around for a while, but it has only been in the last few years that doing so on a cellular level has been feasible. (genengnews.com)

High-throughput extraction, amplification, and detection (HEAD) of HCV-RNA in individual blood donations. (thermofisher.com)
The compact design featuring high power LEDs, large numerical aperture optics, and fast photodiode detection enables all-optical individual well readout of 24-wells simultaneously from multi-well plates while maintaining sufficient temporal resolution to probe millisecond response dynamics. (frontiersin.org)
Title : Newborn Screening Quality Assurance Program for CFTR Mutation Detection and Gene Sequencing to Identify Cystic Fibrosis Personal Author(s) : Hendrix, Miyono M.;Foster, Stephanie L.;Cordovado, Suzanne K. (cdc.gov)

The general approach taken by the document is primarily to provide guidance on how test results might be interpreted based on the outcome of standardised assays. (oecd.org)
The use of column chromatography, and the requirement that each reaction is separated individually, limits the throughput of the approach. (ddw-online.com)

The pattern of metabolic deviations associated with lower birthweight resembled the metabolic signature of higher adult BMI (R 2 = 0.77) assessed at the same time as the metabolic profiling. (nih.gov)
The resemblance indicated that 1 kg lower birthweight is associated with similar metabolic aberrations as caused by 0.92 units higher BMI in adulthood. (nih.gov)

Future studies will begin linking the transwell assay to higher content chemical screening capabilities to increase throughput. (cdc.gov)

Conventional assays using radioisotopes are expensive and time-consuming and have limited the ability of drug researchers to identify potential new drugs via high-throughput screening. (news-medical.net)

To evaluate the Swarm screening system, we optimized a series of heterologous optogenetic spiking HEK293 cell assays for several voltage-gated sodium channel subtypes including Nav1.2, Nav1.5, and Nav1.7. (frontiersin.org)

Anatomy7

Organisms9

Diseases1

Chemicals and Drugs47

Analytical, Diagnostic and Therapeutic Techniques and Equipment99

Phenomena and Processes57

Disciplines and Occupations19

Anthropology, Education, Sociology and Social Phenomena2

Technology, Industry, Agriculture13

Information Science29

Health Care11

Vitro6

Compounds15

Toxicology2

Fluorescence4

Inhibitors3

Biochemical assays1

Development11

Quantitative2

Thermodynamics1

Biosciences2

Methods2

Viability2

Lead optimization1

Efficacy1

Inhibitory2

Flow cytometry1

Robotic1

Enzymes1

Reagents4

Biology1

Predictive1

Libraries3

Molecular6

Organoids1

Cell-based6

Genome1

Reagent2

Functional2

10,0001

Antiviral1

Targets3

Antibodies1

Homogeneous2

Preclinical2

20221

Uptake1

Antibody3

Surface plasmon r1

Drug discovery1

Suitable1

Cells7

Robust2

Stem1

Small molecules1

Sensitivity1

Affinity2

Physiologically relevant1

Automation3

Molecules2

Indirect1

Method1

Data1

Cellular1

Detection3

Approach2

Metabolic2

Capabilities1

Identify1

Sodium1