Hepatocytes. Medical search. Frequent questions

Hepatocytes manufacture their own structural proteins and intracellular enzymes. (wikipedia.org)
The endoplasmic reticulum (ER) is involved in conjugation of proteins to lipid and carbohydrate moieties synthesized by, or modified within, the hepatocytes. (wikipedia.org)
Proteins produced by hepatocytes that function as hormones are known as hepatokines. (wikipedia.org)
To generate a suite of liver proteins adducted by TA metabolites for proteomic analysis, and to reduce the need for both animals and labeled compounds, we treated isolated hepatocytes directly with TA. (ku.edu)
Selective induction of P450e by kepone in the hepatocyte cultures, the first pharmacologic dissociation of the induction of P450b and P450e mRNAs and proteins, was not apparent in kepone-treated rats, where both P450b and P450e mRNAs were increased to equivalent extents. (aspetjournals.org)
Selective protein adducts to membrane proteins in cultured rat hepatocytes exposed to diclofenac: radiochemical and immunochemical analysis. (aspetjournals.org)
The nonsteroidal anti-inflammatory drug diclofenac can be bioactivated to the reactive acyl glucuronide, which covalently binds to hepatocellular proteins in rat hepatocytes. (aspetjournals.org)
There were no detectable cross-reactive epitopes of proteins recognized by the antibody on Western blots of cultured hepatocytes not treated with diclofenac. (aspetjournals.org)
Collectively, these results suggest that binding of diclofenac to rat hepatocyte proteins is selective and that a 60-kDa microsomal membrane protein (or protein subunit) that accumulates in the plasma membrane fraction appears to be the major target for alkylation both in cultured hepatocytes exposed to diclofenac and in vivo. (aspetjournals.org)
The aim of the current thesis was to determine the effect of induction of stress proteins on the subsequent toxicity of chemicals in isolated hepatocytes. (ucl.ac.uk)
The use of stress proteins as markers was problematic as hepatocyte monolayers had elevated basal stress protein levels resulting in poor induction following chemical exposure. (ucl.ac.uk)

As with other tissues, there is a scarcity of donor livers and hepatocytes, which is compounded by the low recovery and proliferative capacity of adult hepatocytes. (news-medical.net)
The present study investigated whether streptozotocin-diabetes could be reversed in FRG mice in which chimeric mouse-human livers can readily be established and, in addition, whether pancreatic transdifferentiation occurred in the engrafted human hepatocytes. (nih.gov)
Strikingly, in vitro-expanded hepatocytes engrafted, and significantly repopulated, the injured livers of Fah(-/-) mice. (ca.gov)
The four CYP2C mRNAs were expressed in human livers and cultured primary hepatocytes, but only the CYP2C18 protein was not detected. (aspetjournals.org)
After acetaminophen administration, SOX9(+) dedifferentiated hepatocytes appeared around the necrotic area, which was suppressed in aged livers. (nii.ac.jp)
Journal Article] Identification and In Vitro Expansion of Adult Hepatocyte Progenitors from Chronically Injured Livers. (nii.ac.jp)

Silencing of TFEB decreases the level of autophagy-related protein LC3BII/I and increases p62 expression level in hepatocytes after exposure to Hcy. (lu.se)
The intracellular lipid level in hepatocytes from fish fed RO diet and DHA diet were higher, and the expressions of the genes for microsomal transfer protein (MTP) and apolipoprotein A1 (Apo A1) were lower, than those in fish fed the two other diets. (nofima.no)

This thesis describes that hepatocyte death and survival are regulated at the cross-roads of intracellular organelles (e.g., mitochondria and endoplasmic reticulum), membrane-bound receptors (such as GPCRs and EGFR) and cell survival signaling pathways (such as ERK, PI3K and PKC). (rug.nl)
In sandwich culture, the intracellular concentrations in Transporter Certified hepatocytes reflect physiologic conditions because the hepatocytes express functioning uptake and efflux transporters and develop bile pockets similar to bile canaliculi. (manufacturingchemist.com)
Specifically, Transporter Certified hepatocytes can be used for transporter studies, quantitation of basolateral versus biliary efflux, drug interaction experiments, intracellular concentration determination, induction, metabolism, hepatotoxicity or any experiment when transporters and in vivo correlation are important. (manufacturingchemist.com)
When hepatocytes were introduced with the intracellular domain of Notch 2 and Grhl2, they transdifferentiated into CK19(+) cholangiocyte-like cells after chronic injury. (nii.ac.jp)

The expression and inducibility of four CYP2C genes, including CYP2C8 , - 2C9 , - 2C18 , and - 2C19 , was investigated in primary cultures of human hepatocytes. (aspetjournals.org)
We conclude that the P450b and P450e genes may be expressed independently by process(es), possibly involving extrahepatic factors, that can be defined with the present hepatocyte culture system. (aspetjournals.org)
Furthermore, drug-induced activation of Nrf2 marker genes was observed when hepatocytes were exposed to test drugs. (edgehill.ac.uk)
Lastly, to better understand the signaling events that result in the rapid downregulation of mature genes in hepatocytes , we examined changes in chromatin accessibility in these cells during the first 24 h of culture using Assay for Transposase-Accessible Chromatin using sequencing ( ATAC-seq ). (bvsalud.org)

As these hepatocytes can mimic liver properties, they allow his team to evaluate the metabolism, drug-drug interaction, and toxicity of drug candidates in addition to their impact on parasite load. (massbio.org)
Three models of hepatocyte cell death were studied: glycochenodeoxycholic acid (GCDCA)-induced cell death as a model for bile acid toxicity, tumor necrosis factor α (TNFα)/ actinomycin D (ActD, an inhibitor of transcription) as a model for cytokine-induced toxicity and menadione-induced cell death (menadione is a superoxide anion donor) as a model for oxidative stress-induced toxicity. (rug.nl)
Metabolism and Toxicity of Thioacetamide and Thioacetamide S-Oxide in Rat Hepatocytes. (ku.edu)
Freshly isolated mouse hepatocytes were used to determine the role of mitochondrial permeability transition (MPT) in acetaminophen (APAP) toxicity. (aspetjournals.org)
To separate metabolic events from later events in toxicity, hepatocytes were preincubated with APAP for 2 h followed by centrifugation of the cells and resuspension of the pellet to remove the drug and reincubating the cells in media alone. (aspetjournals.org)
Inclusion of cyclosporine A, trifluoperazine, dithiothreitol (DTT), or N -acetylcysteine (NAC) in the reincubation phase prevented hepatocyte toxicity. (aspetjournals.org)
Primary hepatocytes, the most common in vitro model for drug-induced liver toxicity testing, are not ideal for high throughput screening due to their limited availability, difficulty to culture, and functional instability. (rndsystems.com)
Hepatocyte-like cells, derived from human pluripotent stem cells, are emerging as a stable and renewable model for drug-induced liver toxicity testing. (rndsystems.com)
Hyperthermic treatment of hepatocytes had a bi-phasic effect on the toxicity of subsequent chemical exposure. (ucl.ac.uk)
Assessment of the roles of P-glycoprotein and cytochrome P450 in triptolide-induced liver toxicity in sandwich-cultured rat hepatocyte model. (curehunter.com)
Human hepatocytes were exposed to three drugs (flucloxacillin, amoxicillin and isoniazid) associated with DILI potentially mediated by the adaptive immune system as drug-specific T-cells have been isolated from DILI patients, and the metabolite nitroso-sulfamethoxazole (SMX-NO). Hepatocyte toxicity, cytokine release and activation of oxidative stress pathways were measured. (edgehill.ac.uk)
Isoniazid which did not induce significant hepatocyte toxicity, compared with SMX-NO and flucloxacillin, stimulated the release of a panel of cytokines including the above and IFN-γ, IL-12, IL-17A, IP-10 and IL-10. (edgehill.ac.uk)

In vitro model systems based on hepatocytes have been of great help to better understand the role of hepatocytes in (patho)physiological processes of the liver. (wikipedia.org)
Arguably, human hepatocytes provide the closest in vitro model to human liver by providing the full complement of xenobiotic-metabolizing enzymes and transporters. (aspetjournals.org)

Here, we produced mice that lacked CB-1 in hepatocytes or stellate cells to determine if CB-1 signaling contributes to the development of NAFLD or liver fibrosis. (jci.org)

These data show that human hepatocytes can be induced to undergo partial pancreatic transdifferentiation in vivo, indicating that the technology holds promise for the treatment of type 1 diabetes. (nih.gov)
However, upon physical or chemical injury to the liver, hepatocytes proliferate extensively in vivo under the direction of multiple extracellular cues, including Wnt and pro-inflammatory signals. (ca.gov)
These studies were performed in two hepatocyte models, hepatocyte monolayers and liver spheroids, and the hepatic effect was also determined in vivo. (ucl.ac.uk)
Glibenclamide uptake into hepatocytes was dramatically decreased in vivo and in vitro. (diabetesjournals.org)
Attempts have been made to better recapitulate the in vivo liver environment in culture , but the full spectrum of signals required to maintain hepatocyte function ex vivo remains elusive. (bvsalud.org)
To elucidate molecular changes that accompany, and may contribute to dedifferentiation of hepatocytes ex vivo, we performed lineage tracing and comprehensive profiling of alterations in their gene expression profiles and chromatin landscape during culture . (bvsalud.org)

Journal Article] Hepatocytic parental progenitor cells of rat small hepatocytes maintain a self-renewal capability after long-term culture. (nii.ac.jp)
Journal Article] Transplantation of Thy1+ cells accelerates liver regeneration by enhancing the growth of small hepatocyte-like progenitor cells via IL17RB signaling. (nii.ac.jp)

We focus on the lineage plasticity of hepatocytes and cholangiocytes during development and regeneration. (nii.ac.jp)

Primary hepatocytes are widely used in the pharmaceutical industry to screen drug candidates for hepatotoxicity, but hepatocytes quickly dedifferentiate and lose their mature metabolic function in culture . (bvsalud.org)

Anisokaryosis (or variation in the size of the nuclei) is common and often reflects tetraploidy and other degrees of polyploidy, a normal feature of 30-40% of hepatocytes in the adult human liver. (wikipedia.org)
The effects of dexamethasone on multiple metabolic functions of adult rat hepatocytes in monolayer culture were studied. (nih.gov)
In the healthy adult liver, most hepatocytes proliferate minimally. (ca.gov)
Selective induction of cytochrome P450e by kepone (chlordecone) in primary cultures of adult rat hepatocytes. (aspetjournals.org)
To critically examine this assumption, we used a new system of primary cultures of adult rat hepatocytes on Matrigel to evaluate whether organochlorine pesticides, as "phenobarbital-like" agents, directly induce these cytochromes in parallel in the liver parenchymal cell. (aspetjournals.org)

1998).Linkage and molecular scaning analysis of MODY 3/hepatocyte nuclear factor 1-alpha gene in typical familial type 2 diabetes: Evidence for novel mutation in exon 8 and 10. (scirp.org)
Diabetes in subjects with hepatocyte nuclear factor (HNF)-1α gene mutations (maturity-onset diabetes of the young [MODY]-3) is characterized by impaired insulin secretion. (diabetesjournals.org)
Mutations in the hepatocyte nuclear factor (HNF)-1α gene cause the most common form of maturity-onset diabetes of the young (MODY), type 3 ( 1 ). (diabetesjournals.org)
The HNF1B gene provides instructions for making a protein called hepatocyte nuclear factor-1 beta (HNF-1β). (medlineplus.gov)

These cells are involved in: Protein synthesis Protein storage Transformation of carbohydrates Synthesis of cholesterol, bile salts and phospholipids Detoxification, modification, and excretion of exogenous and endogenous substances Initiation of formation and secretion of bile The typical hepatocyte is cubical with sides of 20-30 μm, (in comparison, a human hair has a diameter of 17 to 180 μm). (wikipedia.org)
Engraftment of human hepatocytes was confirmed by measuring human albumin levels. (nih.gov)
Quantitative RT-PCR showed expression of human and mouse endocrine hormones and β-cell transcription factors, indicating partial pancreatic transdifferentiation of mouse and human hepatocytes. (nih.gov)
Nonfasting human C-peptide levels were significantly higher than mouse levels, suggesting that transdifferentiated human hepatocytes made a significant contribution to the reversal of diabetes. (nih.gov)
During this webinar, Dr. Steven Maher, an associate research scientist in the Center for Tropical and Emerging Global Diseases Department at the University of Georgia, will outline how his team used primary human hepatocytes to develop the first medium-throughput, 384-well plate-based liver-stage antimalarial drug discovery platform. (massbio.org)
Using this technology, Dr. Maher's team was able to determine the optimal conditions for culturing the liver stages of Plasmodium in primary human hepatocyte cultures. (massbio.org)
They include large lots of highly viable, consistent cryopreserved human hepatocytes, and cynomolgus and rhesus hepatocytes, which have been isolated to increase their robustness and reliability in plated assays. (massbio.org)
Primary human hepatocytes in culture are commonly used to evaluate cytochrome P450 (P450) induction via an enzyme activity endpoint. (aspetjournals.org)
Kinetic analysis of enzyme inactivation in hepatocytes was used to describe the effect of these time-dependent inhibitors and derive the inhibition parameters k inact and K I , which generally were in good agreement with the values derived using recombinant P450s and human liver microsomes (HLMs). (aspetjournals.org)
K I 30 μM and k inact 0.02 min -1 ) effectively abolished CYP2C9 activity over 24 h at low (micromolar) concentrations in primary cultured human hepatocytes. (aspetjournals.org)
recombinantly expressed P450s, human liver microsomes, and human hepatocytes. (aspetjournals.org)
We incubated carfentanil with human hepatocytes for up to 1 h and analyzed samples on a Sciex 3200 QTRAP mass spectrometer to measure parent compound depletion and extrapolated that to represent intrinsic clearance. (diva-portal.org)
Pooled primary human hepatocytes were then incubated with carfentanil up to 6 h and analyzed for metabolite identification on a Sciex 5600+ TripleTOF (QTOF) high-resolution mass spectrometer. (diva-portal.org)
While the human liver microsomal system demonstrated rapid clearance by CYP enzymes, the hepatocyte incubations showed much slower clearance, possibly providing some insight into the long duration of carfentanils effects. (diva-portal.org)
In this study, we demonstrate the reliability and efficiency our StemXVivo ® Hepatocyte Differentiation Kit , which uses a standardized protocol with high quality reagents to generate functional hepatocyte-like cells from human pluripotent stem cells. (rndsystems.com)
Characterisation of drug-specific signalling between primary human hepatocytes and immune cells. (edgehill.ac.uk)
Exposure of human hepatocytes to doses of fipronil (5-amino-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-4-[(trifluoromethyl) sulfinyl]-1H-pyrazole-3-carbonitrile) ranging from 0.1 to 25 µM resulted in a dose dependent increase in CYP1A1 mRNA expression (3.5 to approximately 55-fold) as measured by the branched DNA assay. (cdc.gov)
Cytotoxic effects of fipronil and fipronil sulfone were examined using the adenylate kinase and the trypan blue exclusion assays in HepG2 cells and human hepatocytes. (cdc.gov)
The results indicate both that HepG2 cells and primary human hepatocytes are sensitive to the cytotoxic effects of fipronil. (cdc.gov)
The possibility that cytotoxic effects were due to apoptosis was indicated by significant time- and dose-dependent induction of caspase-3/7 activity in both HepG2 cells and human hepatocytes. (cdc.gov)
When the mosquito feeds on another human, sporozoites are inoculated and quickly reach the liver and infect hepatocytes. (msdmanuals.com)

Hepatocyte cell death can occur via either apoptosis or necrosis (or intermediate forms). (rug.nl)

Hepatocyte nuclei are round with dispersed chromatin and prominent nucleoli. (wikipedia.org)
the volume of hepatocyte nuclei increases from 410 µm 3 at birth to 840 µm 3 at the 12th month and decreases thereafter to 470 µm 3 at the 27th month. (ac.be)

The improved understanding of the metabolism and covalent binding of TA and TASO facilitates the use of hepatocytes to prepare protein adducts for target protein identification. (ku.edu)
Short term cultured rat hepatocytes were used to further study the formation and nature of protein adducts after exposure to diclofenac. (aspetjournals.org)
The antidiclofenac antibody did recognize diclofenac-protein adducts on Western blots of homogenates of cultured rat hepatocytes exposed to diclofenac. (aspetjournals.org)

The hepatocytes exhibited functional hepatic characteristics such as glycogen storage, indocyanine green uptake and release, and albumin secretion. (news-medical.net)
The increased availability of these hepatocytes helps researchers develop new drugs for hepatic infectious diseases and ADME Tox models. (massbio.org)

Deletion of CB-1 in hepatocytes did not alter the development of NAFLD in mice fed a high-sucrose diet (HSD) or a high-fat diet (HFD). (jci.org)
To study this hypothesis in more mechanistic detail, we have established a system of primary hepatocytes isolated from 90 day old mice fed one of 3 diets from weaning as a lipid-relevant perturbagen: high fat (65% kcal/fat), medium fat (45% kcal/fat) and normal chow (10% kcal/fat). (bmj.com)
Given that the survival of mice was remarkably decreased, hepatocyte de-differentiation could be correlated with the protection of liver tissue. (nii.ac.jp)

Through our new partnership with Qualyst, we can assure our customers that the hepatocyte lots we supply have transporter uptake and efflux levels that are physiologically relevant under defined culture conditions. (manufacturingchemist.com)
The aluminium uptake by rat isolated hepatocytes and its effect on oxygen uptake has been investigated. (medicaljournal-ias.org)

Cryopreserved hepatocytes -Monolayer grade. (3hbiomedical.com)

The hepatocyte plates are one cell thick in mammals and two cells thick in the chicken. (wikipedia.org)
The endothelial cells have no basement membrane and are separated from the hepatocytes by the space of Disse, which drains lymph into the portal tract lymphatics. (wikipedia.org)
Advanced Cell Technology, Inc. has reported for the first time a robust and highly efficient process for the generation of high-purity hepatocytes (liver cells). (news-medical.net)
The research, described online (ahead of print) in the journal STEM CELLS , signifies a significant step towards the efficient generation of hepatocytes for use in regenerative medicine and drug discovery. (news-medical.net)
In addition to demonstrating the efficient generation of hepatocytes in research published today, the company has made significant progress in the generation of retinal pigmented epithelial (RPE) cells to treat retinal degenerative diseases and the generation of hemangioblasts to treat vascular disease as well as to create a large-scale and donorless source of red blood cells and platelets. (news-medical.net)
In addition to the cells ' potential use for the treatment of liver disease, hESC-derived hepatocytes could also provide a valuable model for novel pharmaceutical drug discovery assays as well as new drug metabolism and cytotoxicity screens, particularly because the liver is a major site for detoxification. (news-medical.net)
We have established a highly-efficient method for deriving hepatocytes from stem cells that mirrors events in embryonic development, " said Robert Lanza, M.D., Chief Scientific Officer at Advanced Cell Technology, Inc. and senior author of the study. (news-medical.net)
In an animal model of acute liver injury, the hESC-DE cells differentiated into hepatocytes and successfully repopulated the damaged liver. (news-medical.net)
Currently, liver organoids can be generated readily in vitro from bile-duct epithelial cells, but not hepatocytes. (ca.gov)
The intricate LME-hepatocytes signaling pathways through which healthy and diseased liver cells communicate within the LME will be further investigated in our future studies. (confex.com)
Hepatocyte growth factor (HGF) is produced by stromal and mesenchymal cells, and it stimulates epithelial cell proliferation, motility, morphogenesis and angiogenesis in various organs via tyrosine phosphorylation of its cognate receptor, Met. (spandidos-publications.com)
HGF was cloned as a growth factor for hepatocytes ( 1 , 2 ), is identical to scatter factor (SF) and was originally discovered as a fibroblast-derived cell motility factor for epithelial cells ( 3 ). (spandidos-publications.com)
The StemXVivo ® Hepatocyte Differentiation Kit yielded a highly enriched population of pluripotent stem cell-derived hepatocyte-like cells that were functionally characterized by staining for lipid and glycogen storage, quantitative analysis of albumin and urea secretion, and assessment of p450 activity. (rndsystems.com)
These results demonstrate that the StemXVivo ® Hepatocyte Differentiation Kit provides a reliable and renewable source of hepatocyte-like cells that can be utilized for high throughput toxicology and drug discovery. (rndsystems.com)
If the cells were allowed a recovery period prior to chemical exposure the hepatocytes became tolerant to the toxic actions of the chemicals. (ucl.ac.uk)
Here, we showed that hepatocyte growth factor (HGF), a ligand of MET oncoprotein, induces gefitinib resistance of lung adenocarcinoma cells with EGFR -activating mutations by restoring the phosphatidylinositol 3-kinase/Akt signaling pathway via phosphorylation of MET, but not EGFR or ErbB3. (aacrjournals.org)
Since cross-talk between hepatocytes and immune cells is likely to be critical in determining the outcome of drug exposure, the aim of this study was to profile the signals released by drug-treated hepatocytes and to characterise the impact of these molecules on dendritic cells. (edgehill.ac.uk)
Incubation of dendritic cells with supernatant from drug-treated hepatocytes resulted in two distinct cytokine profiles. (edgehill.ac.uk)
Collectively, our study identifies drug-specific signalling pathways between hepatocytes and immune cells that could influence whether drug exposure will result in an immune response and tissue injury. (edgehill.ac.uk)
First, using genetically tagged hepatocytes we demonstrate that expression of the fetal gene alpha-fetoprotein in cultured hepatocytes comes from cells that previously expressed the mature gene albumin , and not from a population of albumin -negative precursor cells , proving mature hepatocytes undergo true dedifferentiation in culture . (bvsalud.org)

In this study, macrophages (J774) and hepatocytes (HepG2) were stimulated with lipopolysaccharide (LPS) from E. coli 0111: B4 and analyzed for modulation of this response when preconditioned or stimulated subsequent to LPS, with different doses of Vitamin D 3 or docosahexaenoic acid (DHA) over a time period of 1 and 5 days. (frontiersin.org)
The maximum induction of adenylate kinase was ca. 3-fold greater than the respective controls in HepG2 and 6-10-fold in the case of primary hepatocytes. (cdc.gov)

Transporter Certified is an industry standard that ensures hepatocytes have physiologic transporter function, metabolic competence and appropriate regulatory pathways under conditions Qualyst has defined. (manufacturingchemist.com)

Furthermore, we found that part of hepatocyte express SOX9 during formation of hepatocellular carcinoma induced by diethylnitrosamine. (nii.ac.jp)

Signal transduction pathways in hepatocyte cell death. (rug.nl)

We have therefore investigated the effects of rapeseed oil (RO) and n-3 rich diets on the accumulation and secretion of H-3-glycerolipids by salmon hepatocytes. (nofima.no)
Secretion of hepatocyte glycerolipids was lower in fish fed the EPA diet and DHA diet than it was in fish fed the RO diet. (nofima.no)

Because rodents typically feed at night, the degree of glycogen accumulation within hepatocytes is typically highest in the early morning hours and wanes throughout the day. (nih.gov)
There is a notable depletion of glycogen within hepatocytes. (nih.gov)
The morphologic appearance of glycogen in hepatocytes is distinctive, allowing glycogen accumulation or depletion to be readily diagnosed in most cases. (nih.gov)
If, in contrast to the control animals, there is a treatment-related increase in glycogen in the hepatocytes, glycogen accumulation should be diagnosed in the treated animals and assigned a severity grade. (nih.gov)
It was also demonstrated that PTPϵM suppressed insulin-induced glycogen synthesis and inhibited insulin-induced suppression of phosphoenol pyruvate carboxykinase (PEPCK) expression in primary hepatocytes. (bioone.org)

Heinz David , «Quantitative characterisation of ageing hepatocytes», Acta Stereologica [En ligne], Volume 2 (1983), Number 2 - Proceedings of the second symposium on morphometry in morphological diagnoses, september 7-9, 1983, Kuopio, Finland - Dec. 1983, 408-412 URL : https://popups.ulg.ac.be/0351-580x/index.php?id=4314. (ac.be)

Steatosis is the accumulation of fat in hepatocytes which can lead to an overall increase in liver size, so-called hepatomegaly. (frontiersin.org)

Developing standardized differentiation protocols that reliably generate functional hepatocytes is important for the future use of stem cell-derived hepatocytes for toxicology and drug discovery. (rndsystems.com)

Single-cell RNA sequencing reveals broad expression of hepatocyte markers. (ca.gov)

A hepatocyte is a cell of the main parenchymal tissue of the liver. (wikipedia.org)
Induced pluripotent stem cell derived hepatocytes (i-Heps) afford us the exciting possibility of redressing the growing shortage in liver transplantation tissue and heralding in a new era of patient specific drug and disease modelling. (europa.eu)
Under a magnification of 500X, 4X greater than that seen in PHIL 22996, this photomicrograph of a rhesus monkey liver tissue specimen, revealed the presence of an enlarged hepatocyte (arrow), which had been infected by the parasitic organism, Plasmodium cynomolgi . (cdc.gov)
The parasites mature into tissue schizonts within hepatocytes. (msdmanuals.com)

Clinical applications are restricted by their poor functionality in comparison to the gold standard - freshly isolated primary hepatocytes. (europa.eu)

Inflammatory Cytokine TNFalpha Promotes the Long-Term Expansion of Primary Hepatocytes in 3D Culture. (ca.gov)
Here, we show that TNFalpha, an injury-induced inflammatory cytokine, promotes the expansion of hepatocytes in 3D culture and enables serial passaging and long-term culture for more than 6 months. (ca.gov)

These hepatocytes perform at the highest level because we uniquely characterise and certify their transporter and bile acid function. (manufacturingchemist.com)

He is an expert both in Plasmodium culture techniques, which are applicable throughout the parasite's lifecycle (blood, mosquito, and liver stages), and complex hepatocyte culture models. (massbio.org)
During his webinar, Dr. Maher will discuss the drug discovery platform, review complex and multiplex in vitro systems for primary hepatocyte culture, describe how to assess primary hepatocytes lots to determine their suitability for Plasmodium infection, and outline next steps in understanding the Plasmodium lifecycle. (massbio.org)
Using binding motif analysis of the areas of open chromatin sharing similar temporal profiles, we identify several candidate transcription factors potentially involved in the dedifferentiation of primary hepatocytes in culture . (bvsalud.org)

The hepatocyte is a cell in the body that manufactures serum albumin, fibrinogen, and the prothrombin group of clotting factors (except for Factors 3 and 4). (wikipedia.org)
We further demonstrated that hepatocyte cultured on soft substrate showed better maintenance of hepatocyte-specific functions of urea and albumin synthesis. (confex.com)

Conclusions We identified a missense mutation of MET , encoding the hepatocyte growth factor receptor, as a likely cause of hearing loss in humans. (bmj.com)

Hepatocyte nuclear factors are a family of evolutionarily conserved transcription factors that are preferentially expressed in HEPATOCYTES. (umassmed.edu)

Incubation of APAP (1 mM) with hepatocytes resulted in cell death as indicated by increased alanine aminotransferase in the media and propidium iodide fluorescence. (aspetjournals.org)
The hepatocytes aluminium content has elevated after incubation in medium containing aluminium-transferrin (Al-tf) or free aluminium (ultrafiltrable). (medicaljournal-ias.org)

SIGNIFICANCE STATEMENT We found the roles of hepatocyte growth factor (HGF) signaling in stria vascularis development for the first time and that lack of HGF signaling in the inner ear leads to profound hearing loss in the mouse. (jneurosci.org)

Both groups of diseases are characterized by a gradual and progressive loss of viable hepatocytes leading to liver inflammation, fibrogenesis and end-stage liver disease. (rug.nl)

One of the detoxifying functions of hepatocytes is to modify ammonia into urea for excretion. (wikipedia.org)

Rapid changes in chromatin structure during dedifferentiation of primary hepatocytes in vitro. (bvsalud.org)

G (p.F841V) in MET (mesenchymal epithelial transition factor), which encodes the receptor for hepatocyte growth factor. (bmj.com)

Hepatocyte and immune system: acute phase reaction as a contribution to early defence mechanisms. (bmj.com)

Qualyst's patented B-CLEAR Technology is used to evaluate hepatocyte lots and qualify them as Transporter Certified. (manufacturingchemist.com)

Significantly, we found that hepatocytes produce TA from TASO relatively efficiently by back-reduction. (ku.edu)

Hepatocytes have the ability to metabolize, detoxify, and inactivate exogenous compounds such as drugs (see drug metabolism), insecticides, and endogenous compounds such as steroids. (wikipedia.org)
ET on March 4 that will describe how primary hepatocytes are being used to screen small molecule drugs for activity against the Plasmodium parasites that cause malaria. (massbio.org)

The aim of this thesis is to elucidate the mechanisms of cell death in hepatocytes, in order to develop strategies to protect hepatocytes and prevent liver injury. (rug.nl)

In characterizing the molecular differentiation of developing peripheral auditory structures, we discovered that hepatocyte growth factor ( Hgf ) is expressed in the future stria vascularis of the cochlear epithelium. (jneurosci.org)
Hepatocyte growth factor (HGF). (spandidos-publications.com)

Primary hepatocytes are commonly used in cell biological and biopharmaceutical research. (wikipedia.org)

Anatomy24

Organisms16

Diseases22

Chemicals and Drugs120

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Phenomena and Processes35

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