• cells
  • A glycosaminoglycoside (GAG) composition predominantly of tetrasacchardie units derived from heparin/heparan sulfate has enchanced activity in preventing the proliferation of smooth muscle cells. (google.com)
  • This staining was not seen in the case of immunolabeling of live cells (Fig. 2 D), suggesting that some heparan sulfates are associated with intracellular compartments. (nih.gov)
  • cell
  • Cell surface heparan sulfate proteoglycan syndecan-2 induces the maturation of dendritic spines in rat hippocampal neurons. (nih.gov)
  • We demonstrate that the cell surface heparan sulfate proteoglycan syndecan-2 plays a critical role in spine development.Deletion of the COOH-terminal EFYA motif of syndecan-2, the binding site for PDZ domain proteins, abrogates the spine-promoting activity of syndecan-2.Our results indicate that syndecan-2 plays a direct role in the development of postsynaptic specialization through its interactions with PDZ domain proteins. (nih.gov)
  • We demonstrate that the cell surface heparan sulfate proteoglycan syndecan-2 plays a critical role in spine development. (nih.gov)
  • Cell surface heparan sulfate immunoreactivity is detected on the cell bodies and along dendrites of the pyramidal neurons. (nih.gov)
  • D and E) High-power confocal image shows cell surface heparan sulfate immunoreactivity (yellow) as a puncta on the surface of MAP2-positive dendrites (red), in patterns and shapes reminiscent of dendritic spines. (nih.gov)
  • Double staining for heparan sulfate and either MAP2, a dendritic marker (Fig. 3, A-E), or NCAM (Fig. 3, F-H) further demonstrated a punctate distribution of cell surface heparan sulfate along the dendrites of hippocampal neurons. (nih.gov)
  • Double
  • Bar, 20 μm in A and 10 μm in D and E. (F-H) Double immunostaining of 30 DIV hippocampal neurons with anti-NCAM (red) and anti-heparan sulfate 10E4 (green) antibodies. (nih.gov)
  • Double labeling of methanol-fixed 30 DIV hippocampal neurons with antibodies to heparan sulfate and PSD-95 further confirmed the synaptic localization of heparan sulfate. (nih.gov)
  • similar
  • On the other hand, syndecan-2 is expressed in neurons in a time course and a pattern similar to those of heparan sulfate immunoreactivity. (nih.gov)
  • responsible
  • 1998). These observations further support our finding that syndecan-2 is one of the HSPGs responsible for the spine-specific accumulation of heparan sulfate on mature hippocampal neurons in vitro, though a contribution by unknown HSPGs has not been ruled out. (nih.gov)