• Furthermore, silencing PrP c expression with si-PRNP amplified the fucoidan-induced changes in cell proliferation, apoptosis, and migration. (iiarjournals.org)
  • Proliferation of the Human Colon Carcinoma Cell Line HT29: Autocrine Growth and Deregulated Expression of the c-myc Oncogene" (PDF). (wikipedia.org)
  • Furthermore, we show that the ligands Wnt5a/b are upstream regulators of the non-canonical signature and moreover regulate proliferation of cancer cells in a β-catenin-independent manner. (nature.com)
  • Our experiments indicate that colon cancer cells are dependent on both β-catenin-dependent and -independent Wnt signaling routes for growth and proliferation. (nature.com)
  • Administration of insulin increased colon cancer cell proliferation and polyp formation in Azoxymethane (AOM)-induced cancer model ( 19 , 20 ). (amegroups.org)
  • The activation of PI3K/Akt pathway can increase cell survival, cell growth and proliferation ( 22-24 ). (amegroups.org)
  • We tested the effects of hot water extracts of liquid-phase and solid-phase commercially grown CS on its ability to influence proliferation (using Alamar blue, an oxidation/reduction indicator), migration (serial-wounded monolayer photomicroscopy), invasion through collagen gel (fluorometric assay) and indomethacin-induced apoptosis (active caspase-3 colorimetric assay) of human colon cancer HT29 cells. (edu.au)
  • The Effect of Avidin on Viability and Proliferation of Colorectal Cancer Cells HT-29. (bvsalud.org)
  • The aim of this study was to analyze the effect of avidin treatment on cell viability , proliferation and cyclin D1 expression in colorectal cancer cells HT-29. (bvsalud.org)
  • Colorectal cancer cell line HT-29 incubated with 50, 100, 150, and 200 µg/mL of avidin concentration during 24, 48, and 72 hours, then the cell viability and proliferation were analyzed. (bvsalud.org)
  • The cell viability was measured by MTS assay and the proliferation was measured by BrdU ( 5-bromo-2'-deoxyuridine ) cell proliferation assay. (bvsalud.org)
  • According to cell viability and proliferation result, we determined the 100 µg/mL avidin concentration for analyzing mRNA and protein of cyclin D1 . (bvsalud.org)
  • We demonstrated that the viability and proliferation of HT-29 cells were significantly decreased in all concentration of avidin treatment compared to control. (bvsalud.org)
  • The cell proliferation showed larger reduction in avidin treatment rather than cell viability . (bvsalud.org)
  • This proves avidin could inhibit proliferation of colorectal cancer cell HT-29 quite well. (bvsalud.org)
  • We concluded that avidin treatment could decrease cell viability and proliferation, accompanied by suppression of cyclin D1 expression in colorectal cells HT-29. (bvsalud.org)
  • Furthermore, effects of the tested extract and fractions were tested on some regulators of cell death and proliferation such as caspase-8, caspase-9, nuclear factor-κB (NF-κB), and nitric oxide (NO). Cisplatin was used as positive control. (ac.ir)
  • We treated TMZ-resistant cells U251-TMZ and T98G-TMZ to normoxia or hypoxia and silenced NUPR1 in hypoxia-treated U251-TMZ and T98G-TMZ cells to assess cell viability, proliferation, apoptosis, LC3-II/LC3-I and p62 expressions, and autophagic flux under different concentrations of TMZ. (techscience.com)
  • In addition, the WSPE inhibited proliferation of HT29 human colon cancer cells. (mdlinx.com)
  • Is the Subject Area "Cell proliferation" applicable to this article? (plos.org)
  • CD326 functions as a homotypic calcium-independent cell adhesion molecule and is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation. (biolegend.com)
  • Cell cycle analysis showed a significant increase in the accumulation of KSO-SFE-treated cells at sub-G1 phase, indicating the induction of apoptosis by KSO-SFE. (hindawi.com)
  • Wu X, Song M, Qiu P, Li F, Wang M, Zheng J, Wang Q, Xu F, Xiao H. A metabolite of nobiletin, 4'-demethylnobiletin and atorvastatin synergistically inhibits human colon cancer cell growth by inducing G0/G1 cell cycle arrest and apoptosis. (umassmed.edu)
  • Induction of cell cycle arrest and apoptosis with downregulation of Hsp90 client proteins and histone modification by 4? (umassmed.edu)
  • Colchicine, nocodazole and taxol were found to induce differentiation and apoptosis in HT29 cells. (tau.ac.il)
  • Apoptosis was characterized by detachment of round cells from the cell layer, condensation of nuclear DNA and annexin V binding to cell surfaces. (tau.ac.il)
  • By reference to an internal standard (methylene diphosphonic acid), absolute changes in 31P-detectable high-energy phosphates in these extracts were determined and correlated with changes in bcl-2 protein levels, cell viability, cell cycle, apoptosis and total cellular glutathione (GSH) (an important defence against DNA damage from alkylating agents). (ucy.ac.cy)
  • Cell viability declined to 25% within 18 h, but apoptosis measured using fluorescence techniques remained in the 1-4% range. (ucy.ac.cy)
  • MFE and doxorubicin exert a cytotoxic effect on human colon cancer HT-29 cell line by probably promoting or induction of apoptosis. (cellmolbiol.org)
  • Data showed considerable cytotoxic and antiproliferative effects of S. plifera on colorectal cell line through induction of apoptosis. (ac.ir)
  • Epidemiologic, animal, and laboratory studies suggest that 5-amino-salicylic acid (5-ASA) protects from the development of CRC by altering cell cycle progression and by inducing apoptosis. (nih.gov)
  • From these assays, it is concluded that D. viscosa does not possess any detectable cytotoxic effect on epidermal cells from mouse (3T3), and a slight cytotoxic effect against HT-29 tumor cells compared to 5-FU. (edu.pe)
  • HT29-Lucia™ AhR cells are engineered from the human HT-29 colon adenocarcinoma cell line for the study of the aryl hydrocarbon receptor (AhR) genomic signaling induction (see details), by monitoring the activity of the Lucia luciferase reporter protein. (invivogen.com)
  • HT29 Cell Line. (invivogen.com)
  • The stability of this cell line for 20 passages following thawing has been verified. (invivogen.com)
  • Previous study has shown that kenaf seed oil extracted by SFE (KSO-SFE) is cytotoxic towards human cervical cancer cell line [ 7 ]. (hindawi.com)
  • HT-29 is a human colon cancer cell line used extensively in biological and cancer research. (wikipedia.org)
  • The HT-29-C1.19A cells were derived from the human colonic cancer cell line HT29. (mbpinc.net)
  • Movement of solutes in this cell line is transepithelial. (mbpinc.net)
  • Establishment and characterization of a KIT‐positive and stem cell factor‐producing cell line, KTHOS, derived from human osteosarcoma. (mbpinc.net)
  • Laboisse CL, Augeron C, Couturier-Turpin MH, Gespach C, Cheret AM, & Potet F. (1982) Characterization of a newly established human gastric cancer cell line HGT-1 bearing histamine H2-receptors. (mbpinc.net)
  • 1984) Emergence of permanently differentiated cell clones in a human colonic cancer cell line in culture after treatment with sodium butyrate. (mbpinc.net)
  • Restoration by Polyethylene Glycol of Characteristics of Intestinal Differentiation in Subpopulations of the Human Colonic Adenocarcinoma Cell Line HT29. (mbpinc.net)
  • These images are of human HT29 colon cancer cells, a cell line that has been widely used for the study of many normal and neoplastic processes. (broadinstitute.org)
  • used the HT29 cell line to screen 4,903 shRNAi constructs (targeting 1,028 human genes) in order to find mitotic regulators. (broadinstitute.org)
  • By means of a recently developed non-invasive ex vivo minimal model based on the interaction of the human enterocyte-like HT29 cell line and fecal slurries, we explored the enterocyte-associated microbiome of 21 Hadza hunter-gatherers and nine urban living Italians. (frontiersin.org)
  • The human colon adenocarcinoma cell line HT29 displays an undifferentiated phenotype under standard growth conditions. (tau.ac.il)
  • Interestingly, the growth rate of the mimicked BRCA2 heterozygous cell line was significantly lower than that of control cells. (ijbs.com)
  • In support of this hypothesis, heterozygosity for a BRCA2 mutation has been shown to cause sensitivity to DNA damage agents and reduced RAD51 focus formation after irradiation in the chicken B cell line DT40 [ 16 ]. (ijbs.com)
  • After HN2 administration, bcl-2 protein levels in the HT-29 cell line rose at 2 h. (ucy.ac.cy)
  • The mechanistic basis for the biological properties of Morus alba flavonoid extract (MFE) and chemotherapy drug of doxorubicin on human colon cancer HT-29 cell line death are unknown. (cellmolbiol.org)
  • The effect of doxorubicin and flavonoid extract on colon cancer HT-29 cell line death and identification of APC gene expression and PARP concentration of HT-29 cell line were investigated. (cellmolbiol.org)
  • The results showed that flavonoid extract and doxorubicin induce a dose dependent cell death in HT-29 cell line. (cellmolbiol.org)
  • Results: Metanolic extract of Umbilicaria decussata showed nearly 50 % HT-29 cell line inhibition at 200 μg/mL tested dose. (ac.ir)
  • Each avidin concentration was conducted together with HT-29 cell line without avidin treatment as a control group . (bvsalud.org)
  • Here, we examined the cytotoxic effect and cell death mechanisms of methanolic extract of S. pilifera and its alkaloid and terpenoid fractions on the HT-29 colorectal cell line. (ac.ir)
  • Moreover, a Topo I-altered cell line (KBSTP2), resistant to SN38, remains sensitive to BN80927, suggesting that a part of the antiproliferative effects of BN80927 are mediated by a Topo I-independent pathway. (aacrjournals.org)
  • A prostatic stromal myofibroblast cell line (WPMY-1) was co-incubated with testosterone (1 µM) and different concentrations of capsaicin (10-100 µM) for 24 and 48 h. (techscience.com)
  • The Anti-Cytokeratin antibody, clone CAM 5.2, is derived from the hybridization of P3/NS-1/1-Ag4-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with the human colorectal carcinoma cell line HT29. (bdbiosciences.com)
  • An epithelial cell line initiated from a human bone metastasis of a grade IV prostatic ADENOCARCINOMA. (bvsalud.org)
  • Extraction from 9 parameters of KSO-SFE was screened for cytotoxicity towards human colorectal cancer cell lines (HT29) and mouse embryonic fibroblast (NIH/3T3) cell lines using MTS assay. (hindawi.com)
  • Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. (ac.ir)
  • Preclinical data reported here show that BN80927 retains Topo I poisoning activity in cell-free assay (DNA relaxation) as well as in living cells, in which in vivo complexes of topoisomerase experiments and quantification of DNA-protein-complexes stabilization, have confirmed the higher potency of BN80927 as compared with the Topo I inhibitor SN38. (aacrjournals.org)
  • Capsaicin (10-100 µM) significantly inhibited testosterone-treated WPMY-1 cell growth at 48 h by MTT assay. (techscience.com)
  • In preclinical research, HT-29 cells have been studied for their ability to differentiate and thus simulate real colon tissue in vitro, a characteristic that has made HT-29 useful for epithelial cell research. (wikipedia.org)
  • This hypothesis is also supported by in vitro data showing an antiproliferative activity of BN80927 on a model of resistance related to the noncycling state of cells (G 0 -G 1 synchronized). (aacrjournals.org)
  • In in vitro studies, the addition of NT to human colon cancer cell lines resulted in significantly increased cancer cell growth and the growth of xenografted human colon cancer cells in mice [ 9 ]. (springer.com)
  • Results: Fucoidan treatment significantly inhibited growth and reduced cyclin and cyclin-dependent kinase (CDK) expression in HT29 colon cancer cells. (iiarjournals.org)
  • Conclusion: Combination of fucoidan with silencing of PrP c has a synergic effect on the inhibition of HT29 colon cancer cell growth. (iiarjournals.org)
  • However, c-myc is deregulated, but may have a relation with the growth factor requirements of HT-29 cells. (wikipedia.org)
  • Though HT-29 cells can proliferate in cell culture lacking growth factors with a doubling time of around 4 days, the doubling time can be reduced to one day with added fetal bovine serum. (wikipedia.org)
  • Levels of p27 were also found to be slightly increased in cells with reduced BRCA2, perhaps contributing to the slower growth rate. (ijbs.com)
  • However, it may be possible that tumor growth can be initiated in heterozygous cells prior to loss of the second, wild-type allele. (ijbs.com)
  • In cell growth assays, BN80927 is a very potent antiproliferative agent as shown by IC 50 values consistently lower than those of SN38 in tumor cell lines as well as in their related drug-resistant lines. (aacrjournals.org)
  • One study found that it could help reduce the growth of colon cancer cells. (webmd.com)
  • This property reduces cell damage and can help to limit the growth and potential spread of cancer cells in the body. (webmd.com)
  • The cells can also be tested in vivo via xenografts with rodents. (wikipedia.org)
  • In vivo, NTSR1 mRNA expression was undetectable in superficial differentiated epithelial cells in histological specimens of normal human colonic epithelium, but there was moderate and strong expression in adenomas and adenocarcinomas respectively. (springer.com)
  • As a result, these cells allow studying the AhR genomic signaling pathway, by monitoring the activity of Lucia luciferase in the cell culture supernatant when using the QUANTI-Luc™ 4 Lucia/Gaussia detection reagent. (invivogen.com)
  • For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. (biolegend.com)
  • Open in a separate windows Physique 1 Fibroblasts induce malignancy cell elongation and motilityA. (abic2004.org)
  • These results demonstrate that fibroblasts induce colon cancer cell elongation and motility. (abic2004.org)
  • Cultured dermal, normal colon or colon cancer fibroblasts have comparative gene expression and activation profiles and induce comparable malignancy cell elongation and motility Next we tested whether fibroblasts isolated from normal colon (CFB) or colon cancer (CAF) tissues were also able to induce malignancy cell elongation and motility. (abic2004.org)
  • The fact that dermal fibroblasts and CFB were able to induce these effects on CRC cells was unexpected, as previous studies exhibited that only freshly isolated CAF, but not normal fibroblasts, induced malignancy progression [21, 22]. (abic2004.org)
  • Open in a separate window Physique 2 Cultured dermal, colon and colon cancer associated fibroblasts induce similarly malignancy cell elongation and motility and have equivalent gene expression and activation profilesA. (abic2004.org)
  • Vacuolated cells show a striking phenotype of a large perinuclear vacuole (nuclear associated vacuole, NAV) that is distinct from vesicles observed by treatment with an autophagy-inducing agent. (nih.gov)
  • We are investigating plant species from the Canadian prairie ecological zone by phenotypic cell assays to discover toxins of biological interest. (nih.gov)
  • Transmission electron microscopy and quantitative cell based assays were used in exploring point (c). (lu.se)
  • Materials and Methods: PrP c expression was suppressed in HT29 human colon cancer cells by utilizing small-interfering RNA (si-PRNP), and cells were subsequently used to study the antiproliferative and anticancer effects of fucoidan treatment of HT29 human colon cancer cells. (iiarjournals.org)
  • The cells proliferate rapidly in media containing suramin, with corresponding high expression of the c-myc oncogene. (wikipedia.org)
  • Increased expression of p53 in the mimicked heterozygous cells was observed, perhaps in response to BRCA2 deficiency. (ijbs.com)
  • mTOR pathway regulates autophagy and incubation with IP6 to HT-29 cells showed decreased expression of p-70S6Kinase, 4-EBP-1 in a time-dependent manner. (um.edu.my)
  • Curcumin suppresses PPARδ expression and related genes in HT-29 cells. (wjgnet.com)
  • We found that hypoxia upregulated NUPR1 expression and autophagy while NUPR1 silencing suppressed hypoxia-induced TMZ resistance and autophagy in glioma cells. (techscience.com)
  • Using the MTT test methods the cytotoxic activity of each extract against colorectal HT-29 and breast MCF-7 cancer cells was assessed. (journalcra.com)
  • Materials and methods: Anticancer activity was assayed with standard MTT colorimetric procedure against MCF-7 and HT-29 cell lines. (ac.ir)
  • Human colorectal cancer (HT29) and mouse embryonic fibroblast (NIH/3T3) cell lines were purchased from the American Type Culture Collection (ATCC), USA. (hindawi.com)
  • Therefore, HT29-Lucia™ AhR cells are highly relevant for studying intestinal microbiota-related ligands for AhR . (invivogen.com)
  • Enhancement by Nano-Diamino-Tetrac of Antiproliferative Action of Gefitinib on Colorectal Cancer Cells: Mediation by EGFR Sialylation and PI3K Activation. (umassmed.edu)
  • Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells such as the GOBLET CELLS. (umassmed.edu)
  • Our results show that CNCM I-3690 can (i) physically maintain modulated goblet cells and the mucus layer and (ii) counteract changes in local and systemic lymphocytes. (nature.com)
  • Kynurenine promotes the goblet cell differentiation of HT-29 colon carcinoma cells by modulating Wnt, Notch and AhR signals. (invivogen.com)
  • Generation of inhibitory peptides against bacteria and HT29 human colon cancer cells improved with [pineapple peel powder] supplementation of yogurt. (mdlinx.com)
  • KSO-SFE at 600/40 showed the strongest cytotoxicity towards HT29 with IC 50 of 200 µg/mL. (hindawi.com)
  • Therefore, this study determined the cytotoxicity of KSO-SFE on colon cancer cells. (hindawi.com)
  • Objective: The purpose of this study is to evaluate the cytotoxicity of Euphorbia tirucalli against MCF-7 breast cancer cells and HT-29 colorectal cancer cells. (journalcra.com)
  • N-hexane extract of Euphorbia tirucallihad a low cytotoxic effect with IC50 values of 631.5 g/mL and 919.13 g/mL against breast MCF-7 and colorectal HT-29 cancer cells, respectively. (journalcra.com)
  • Conclusion: It is important to continue developing Euphorbia tirucalli as a potential anti-breast and anti-colorectal cancer drugs as it exhibits cytotoxic effect against colorectal HT-29 and breast MCF-7 cancer cells. (journalcra.com)
  • Thus, one can measure the fraction of cells infected by infecting two wells with lentiviruses for the same hairpin, treat only one of the wells with puromycin, and count the number of surviving cells in each of the two wells. (broadinstitute.org)
  • Treatment with colchicine or forskolin induced the association of E-cadherin to the cytoskeleton fraction of subconfluent HT29 cells. (tau.ac.il)
  • In this study, we hypothesized that 5-ASA restrains cell cycle progression by activating checkpoint pathways in colorectal cell lines, which would prevent tumor development and improve genomic stability. (nih.gov)
  • In addition, we thoroughly test our cell lines for freeze-thaw recovery. (mbpinc.net)
  • 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. (mbpinc.net)
  • No difference in RAD51 subcellular localization and focus formation was observed between control and mimicked heterozygous cell lines. (ijbs.com)
  • As the activation of PI3K/Akt signal pathway increases the resistance of several cancer cell lines such ovarian, lung cancer to chemotherapeutic drugs ( 7 , 8 ), it is possible that PI3K/Akt may also play a role in the poor prognosis of obesity-associated colon cancer. (amegroups.org)
  • We provide the first report of the effects of extracts prepared from the shrub Symphoricarpos occidentalis in several human cell lines. (nih.gov)
  • In this research, the anticancer effect against MCF-7 and HT-29 cell lines, and antioxidant, antimicrobial and wound healing activities of the lichens extracts have been investigated. (ac.ir)
  • This effect was independent of the hMLH1, hMSH2, and p53 status because it was observed to a similar extent in all cell lines under investigation. (nih.gov)
  • Initially derived in 1964 by Jorgen Fogh from a 44-year-old Caucasian female, HT-29 cells form a tight monolayer while exhibiting similarity to enterocytes from the small intestine. (wikipedia.org)
  • HT-29 cells terminally differentiate into enterocytes with the replacement of glucose by galactose in cell culture, and with the addition of butyrate or acids, the differentiation pathways can be closely studied along with their dependence on surrounding conditions. (wikipedia.org)
  • This effect was less prominent in post confluent cells. (tau.ac.il)
  • The effect of heterozygosity of the BRCA2 gene on human cells remains unclear. (ijbs.com)
  • The ethanol extrac of leaves exhibited a major inhibitory effect compared to different fractions in human colon cancer cells (HT-29). (edu.pe)
  • In the present study the effect of IP6 on autophagy-mediated death by modulating the mTOR pathway in HT-29 colon cancer cells was studied. (um.edu.my)
  • Our previous results indicate that 5-ASA improves replication fidelity in colorectal cells, an effect that is active in reducing mutations. (nih.gov)
  • HT29 Cells" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
  • Here, BRCA2 heterozygosity was mimicked in HT-29 colon cells by reducing levels of BRCA2 through stable RNA interference. (ijbs.com)
  • In this study, exosomes released from HeLa cervical cancer cells were loaded with raloxifene to increase its bioavailability, and an aptamer was attached to the exosome membrane for targeting only HeLa cells. (techscience.com)
  • HT29-Lucia™ AhR cells are resistant to Zeocin® . (invivogen.com)
  • Puromycin In addition to delivering the hairpin, the lentivirus makes the cells resistant to the antibiotic puromycin. (broadinstitute.org)
  • The infection is considered successful if at least 25 percent of the cells are resistant to puromycin. (broadinstitute.org)
  • Results: Ethanol extract of Euphorbia tirucalli exhibited a moderate cytotoxic effects with an IC50 value of 33.88 g/mL toward breast MCF-7 cancer cells, and 34.86 g/mL toward colorectal HT-29 cancer cells, respectively. (journalcra.com)
  • The characterization of the resulting cells with regard to key cellular BRCA2 functions is presented here. (ijbs.com)
  • Characterization of exosomes isolated from HeLa cells was performed by transmission electron microscopy, zeta sizer, and western blotting. (techscience.com)