Geobacillus stearothermophilus (previously Bacillus stearothermophilus) is a rod-shaped, Gram-positive bacterium and a member of the phylum Bacillota. (wikipedia.org)
It was first described in 1920 as Bacillus stearothermophilus, but, together with Bacillus thermoglucosidasius, it was reclassified as a member of the genus Geobacillus in 2001. (wikipedia.org)
We conclude that differences in the Hoogsteen hydrogen bonding between nucleotides is the main factor in the preferential selectivity of dTTP opposite O(6)-methylG by human pol ι, in contrast to the mispairing modes observed previously for O(6)-methylG in the structures of the model DNA polymerases Sulfolobus solfataricus Dpo4 and Bacillus stearothermophilus DNA polymerase I. (nih.gov)
Dive into the research topics of 'Proteins of the Bacillus stearothermophilus ribosome. (elsevierpure.com)
Methods A sample study was conducted with 100 dental clinics, by way of a questionnaire about procedures related to the sterilization process and implementation of biological monitoring of the equipment, using spores of Bacillus subtilis for the oven and Geobacillus stearothermophilus for the autoclaves. (bvsalud.org)
Organism1
The biological indicator (BI) organism used in the NO 2 sterilization process is the spore of Geobacillus stearothermophilus, the traditional indicator organism used in both steam and hydrogen peroxide sterilization methods. (mddionline.com)
Spores6
Spores of Geobacillus stearothermophilus are very sensitive to changes in temperature. (pda.org)
When validating sterilizing processes, the most common bioindicator (BI) is spores of Geobacillus stearothermophilus ATCC12980 and ATCC7953 with about 10 6 spores /BI and a D 121 -value of about 2 minutes in water. (pda.org)
Because these spores of Geobacillus stearothermophilus do not survive at a F 0 -value above 12 minutes, it has not been possible to evaluate the agreement between the biological F -value ( F BIO ) and physical measurements (time and temperature) when the physical F 0 -value exceeds that limit. (pda.org)
However, it has been proven that glycerin substantially increases the heat resistance of the spores, and it is possible to utilize that property when manufacturing BIs suitable to use in processes with longer sterilization time or high temperature (above 121°C). By the method described, it is possible to make use of the sensitivity and durability of Geobacillus stearothermophilus' spores when glycerin has increased both test range and accuracy. (pda.org)
The biological indicator with suspension contains viable bacteriene spores of Geobacillus Stearothermophilus, for verification of sterilization in the autoclave. (vetro.ro)
The presence of Geobacillus stearothermophilus spores is detected by a visual color change (turns yellow). (vetro.ro)
HYDROLASE1
The aim of this study was to clone, express, partially characterise and apply a glycoside hydrolase (GH) family 10 xylanase (XT6), derived from Geobacillus stearothermophilus T6, as an additive to locally produced chicken feeds. (ru.ac.za)
Bacteria1
This enzyme has been reported in the bacteria Geobacillus stearothermophilus, Enterobacter aerogenes and Klebsiella pneumoniae. (expasy.org)
Clostridium1
In addition, Clostridium difficile and Geobacillus stearothermophilus were placed in discs at 5 sites in the patient rooms prior to decontamination. (medscape.com)
Sterilization1
Geobacillus stearothermophilus is used for steam sterilization monitoring. (steris-ast.com)
Species1
Combined Species Spore Strips - G. stearothermophilus* (105) and B. atrophaeus* (106) on each strip. (mesalabs.com)
Thermophilic2
Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. (wikipedia.org)
Here, we show that the Cas9 protein from the thermophilic bacterium Geobacillus stearothermophilus (GeoCas9) catalyzes RNA-guided DNA cleavage at elevated temperatures. (doe.gov)
Genus1
Taxonomic revision of the genus Geobacillus: emendation of Geobacillus, G. stearothermophilus, G. jurassicus, G. toebii, G. thermodenitrificans and G. thermoglucosidans (nom. (wikipedia.org)
Viable1
In our previous studies, we carried out the preliminary biofilm experiments, including pellicle formation, complex exopolysaccharide production, biofilm morphotypes, and viable biofilm cell counting on stainless steel of Geobacillus sp. (sciendo.com)
Reduction1
Validated using Geobacillus stearothermophilus for a consistant 99.9999% 6-log and greater reduction. (steramist.com)
Crystal2
High-resolution crystal structures of α-L-arabinofuranosidase from Geobacillus stearothermophilus T-6, a family 51 glycosidase, are described. (huji.ac.il)
Crystal structure of pyruvate kinase from Geobacillus stearothermophilus. (nih.gov)
Activity1
In 2013, a thermostable group II intron reverse transcriptase (TGIRT), GsI-IIC-MRF, from G. stearothermophilus was found to retain activity up to 70 °C and to exhibit high processivity and a low error rate. (wikipedia.org)