• cDNA
  • I'm trying to locate any axolotl cDNA or genomic libraries (preferably Ambystoma mexicanum) and I would be very grateful for any help from members of the urodeles newsgroup. (bio.net)
  • For the cDNA libraries I am particularly interested in embryonic stages (up to tailbud) and in adult skeletal or cardiac muscle. (bio.net)
  • The objective of the proposal is to develop procedures for preparing siRNA libraries using genomic DNA and cDNA samples as the source material for siRNA templates. (sbir.gov)
  • Finally, S1P4 and S1PR5 were cloned from in vitro differentiated human dendritic cells and rat cDNA library. (wikipedia.org)
  • gene
  • We would like to clone the myosin gene(s) from another insect species and are looking for a genomic library from any non-Dipteran insect. (bio.net)
  • The siRNA vector libraries should enable mutation/selection experiments to be used to correlate gene function and identity in mammalian cells. (sbir.gov)
  • Genomic analyses revealed CI-HHV-6A retains the full HHV-6A gene complement, with no interrupted open reading frames, and important cis acting signals likely required for any viral replication/reactivation mechanism. (bl.uk)
  • It is based on the principle that the expected average between-marker distances can be smaller than the average insert length of a clone library containing the gene of interest. (wikipedia.org)
  • As a result, the mapping paradigm has now changed such that one first isolates one or more DNA marker(s) at a physical distance from the targeted gene that is less than the average insert size of the genomic library being used for clone isolation. (wikipedia.org)
  • The DNA marker is then used to screen the library and isolate (or 'land' on) the clone containing the gene, without any need for chromosome walking and its associated problems. (wikipedia.org)
  • sequences
  • For example, they can seek out specific DNA chains in the library with the use of probes which are designed to identify and tag specific amino acid sequences. (wisegeek.com)
  • plaques
  • The number of viral plaques are counted and can be used to calculate the total number of infectious viral particles in the library. (wikipedia.org)
  • identify
  • This is a retrospective pilot study, in which we attempt to identify genomic biomarkers predictive of recurrence in stage I lung cancer by analysing copy number (CN) data obtained by next-generation sequencing. (bl.uk)
  • This study aimed to identify genomic regions of EHEC O157:H7 that contribute to colonization and multiplication at this site. (frontiersin.org)
  • total
  • Conclusion: Although no single chromosomal region was associated with cancer recurrence, the two groups were distinguishable with an algorithm that assesses total genomic change. (bl.uk)
  • tissue
  • To address this, next generation sequencing (NGS) methods were established, to define a readily utilisable pipeline for genomic sequencing of these herpesviruses directly from cell or tissue samples. (bl.uk)
  • species
  • Despite the great advances in genomic technology observed in several crop species, the availability of molecular tools such as microsatellite markers has been limited in melon ( Cucumis melo L.) and cucurbit species. (biomedcentral.com)
  • size
  • Count colony forming units (CFU) of transformants the next day and calculate the size of the libraries (1 x 10 5 CFU/ml of cell or more is good library size). (bio-protocol.org)
  • screen
  • We are looking for a tobacco genomic library to screen and do not want to pay the companies for what is already available (or reinvent the wheel). (bio.net)