• Methylation
  • To test whether a methylation-sensitive restriction system contributes to poor B. burgdorferi transformability, shuttle plasmids were treated with the CpG methylase M.SssI prior to the electroporation of a variety of strains harboring different putative R-M systems. (umassmed.edu)
  • mutants
  • We also find fusions of the ventral neurocranium for all midline mutants tested, which may reveal a hitherto unrecognized function of the midline in influencing differentiation of neural crest cells at their destination. (zfin.org)
  • We have taken a genetic approach to dissecting acquired thermotolerance by characterizing loss-of-function thermotolerance mutants in Arabidopsis. (plantphysiol.org)
  • Tests involving A1 mutants with various deleted amino acid sequences have shown that editing activity is dependent on residues 14 to 35. (wikipedia.org)
  • Molecular
  • This is, to our knowledge, the first molecular genetic characterization of NBAD hydrolase and carcinine hydrolase activity in any organism and is central to the understanding of pigmentation and photoreceptor function. (nih.gov)
  • GRE Subject Biochemistry, Cell and Molecular Biology was a standardized exam provided by ETS (Educational Testing Service) that was discontinued in December 2016. (wikipedia.org)
  • Molecular phylogenetic analysis revealed P. stipticus to have a close genetic relationship with members of the genus Mycena. (wikipedia.org)
  • Reflecting this cross-species genetic complementation method, the gene was called "Excision repair cross-complementing 4" The human ERCC4 gene encodes the XPF protein of 916 amino acids with a molecular mass of about 104,000 daltons. (wikipedia.org)
  • arises
  • Further, the selective advantage of complementation that arises from outcrossing may largely account for the general avoidance of inbreeding in nature (e.g. see articles Kin recognition, Inbreeding depression and Incest taboo) [citation needed]. (wikipedia.org)
  • strains
  • Low-passage-number infectious strains of B. burgdorferi exhibit extremely low transformation efficiencies-so low, in fact, as to hinder the genetic study of putative virulence factors. (umassmed.edu)
  • It attributes the poor performance of inbred strains to loss of genetic diversity, with the strains becoming purely homozygous at many loci. (wikipedia.org)
  • loci
  • Genetic tests demonstrate that Rmr6 encodes a common component required for establishing paramutations at diverse maize loci. (genetics.org)
  • Genetic-linkage studies were able to map the disease loci in cystic fibrosis to chromosome 7 by using protein markers. (wikipedia.org)
  • In general, epistasis is used to denote the departure from 'independence' of the effects of different genetic loci. (wikipedia.org)
  • forward genetic
  • Depending on the region being assessed, forward genetic screens can be classified as illustrated in Figure 2 as: Region Specific screens: Studies are designed specifically to obtain a gradient of phenotypes by generating an allelic series which are helpful in studying the region of interest. (wikipedia.org)
  • Mice
  • A/J inbred mice are widely used to model cancer and for carcinogen testing given their high susceptibility to carcinogen-induced tumors. (jax.org)
  • yeast
  • Furthermore, we examined the interaction between MdCRY2 and AtCOP1 using a yeast two-hybrid assay and a bimolecular fluorescence complementation assay. (biomedsearch.com)
  • His Ph.D. advisor at MIT was David Botstein, and the title of his thesis was "Genetic analysis of the yeast microtubule cytoskeleton. (wikipedia.org)
  • functional
  • Here, we describe the use of nuclear magnetic resonance (NMR)-based ligand screening as a tool for testing functional assignments of putative enzymes that may be of variable reliability. (biomedcentral.com)
  • Firstly, they would allow efficient testing of functional assignments that may be of variable reliability. (biomedcentral.com)
  • variation
  • August Weismann picked up the thread in 1889, arguing that sex served to generate genetic variation, as detailed in the majority of the explanations below. (wikipedia.org)
  • mutant
  • Deletion tests with mutant strands have shown that residues 181 to 210 are integral to mRNA editing, and there is most likely a beta-turn at proline residues 190 and 191. (wikipedia.org)
  • manipulation
  • Biotechnology - new and sometimes controversial branch of biology that studies the manipulation of living matter, including genetic modification and synthetic biology. (wikipedia.org)
  • Evolutionary
  • Since hypotheses for the origins of sex are difficult to test experimentally (outside of Evolutionary computation), most current work has focused on the maintenance of sexual reproduction. (wikipedia.org)
  • data provide
  • These data provide the first direct genetic evidence, to our knowledge, that distinct functions, independent of Hsp synthesis, are required for thermotolerance, including protection of membrane integrity and recovery of protein activity/synthesis. (plantphysiol.org)
  • method
  • This article discusses a method that is applicable to the first level of testing current sequence-based annotations of enzymatic function. (biomedcentral.com)
  • respectively
  • however, in recent versions of the test, the maximum and minimum reported scores have been 760 (corresponding to the 99 percentile) and 320 (1 percentile) respectively. (wikipedia.org)
  • case
  • For a number of case studies, additional experiments (such as in vivo genetic complementation) were performed to determine function so that the reliability of the NMR screen could be independently assessed. (biomedcentral.com)