• One example is adoptive cell therapy with T cells that were genetically modified with gamma-retroviral and lentiviral (LV) delivery vectors to express a CD19-specific chimeric antigen receptor (CAR) for cancer treatment. (uni-wuerzburg.de)
  • In addition, due to the great diversity of immune cell antigen receptor recombination breakpoints, only ~85% of all clonal T-cell populations will be identified with the primers utilized in this assay. (ohsu.edu)
  • Human T-cell antigen receptor gene T3-delta. (lu.se)
  • The gene structure and expression of the non-specific cytotoxic cell receptor protein (NCCRP-1) in Atlantic cod (Gadus morhua L. (nofima.no)
  • Epidermal growth factor receptor in non-small-cell lung carcinomas: correlation between gene copy number and protein expression and impact on prognosis. (nih.gov)
  • Protein expression was assessed by immunohistochemistry on a scale from 0 to 400 (percentage of positive cells x staining intensity). (nih.gov)
  • It will be important to evaluate EGFR gene and EGFR protein status and signal protein expression to properly interpret future clinical trials using EGFR inhibitors. (nih.gov)
  • T-cell receptor (TCR)-induced IL-2 expression is suppressed at the level of mRNA and protein following chronic culture of T cells with tumour necrosis factor (TNF) at picomolar concentrations, an effect that is reversible upon removal of TNF. (biomedcentral.com)
  • Here we demonstrate that it binds Patched (vPtc), which has been identified as a tumour-suppressor protein in basal cell carcinoma, with high affinity. (nih.gov)
  • In the simplest receptors, polypeptide chains cross the lipid bilayer once, while others, such as the G-protein coupled receptors, cross as many as seven times. (wikipedia.org)
  • In some receptors, such as the nicotinic acetylcholine receptor, the transmembrane domain forms a protein pore through the membrane, or around the ion channel. (wikipedia.org)
  • Receptor protein: cells must have cell surface receptor proteins which bind to the signaling molecule and communicate inward into the cell. (wikipedia.org)
  • Binding of the signal molecule to the receptor protein will activate intracellular signaling proteins that initiate a signaling cascade. (wikipedia.org)
  • Our approach pairs sparse scATAC-seq data with robust detection of hundreds of cell surface and intracellular protein markers and optional capture of mitochondrial DNA for clonal tracking, capturing three distinct modalities in single cells. (nature.com)
  • ASAP-seq uses a bridging approach that repurposes antibody:oligonucleotide conjugates designed for existing technologies that pair protein measurements with single-cell RNA sequencing. (nature.com)
  • A collaboration of scientists from Earle A. Chiles Research Institute of the Providence Cancer Institute and the National Cancer Institute previously identified T-cell receptors that target KRAS G12D, a "hot spot" signaling protein mutation found in certain cancers. (cytologicsbio.com)
  • To engineer T cells targeting mutant KRAS protein, T-cell receptors were isolated from the previously treated patient with colorectal cancer, synthesized, and cloned for use to transduce the PBMCs. (cytologicsbio.com)
  • Furthermore, we analysed the 5'-flanking region of the human adipose differentiation-related protein ( adrp ) gene that responded to all subtypes of PPARs. (biomedcentral.com)
  • IFN-γ increased P2X 4 -receptor mRNA and protein, accompanied by an increase in ATP-triggered membrane current. (elsevierpure.com)
  • Wucherpfennig, K.W. and Hafler, D.A. (1995) A review of T-cell receptors in multiple sclerosis: Clonal expansion and persistence of human T-cells specific for an immunodominant myelin basic protein peptide. (scirp.org)
  • Configuration of immunoglobulin and T cell receptor beta and gamma genes in acute myeloid leukaemia: pitfalls in the analysis of 40 cases. (bmj.com)
  • Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98-3936. (ohsu.edu)
  • Since the SLAMF low signature was strongly associated with an unmutated CLL immunoglobulin heavy chain (IGHV) status in large datasets, we investigated the impact of SLAMF1 and SLAMF7 on the B cell receptor (BCR) signaling axis. (nature.com)
  • Rearrangement of the genes for the beta and gamma chains of the T cell receptor is rarely observed in adult B cell lymphoma and chronic lymphocytic leukemia. (jci.org)
  • In 7 of the 100 B cell specimens, a single nongermline band was detected after digestion with the restriction enzyme BamHI, but the rearrangement could be confirmed with a second restriction enzyme in only two. (jci.org)
  • A rearrangement of the T gamma gene was never seen in a B cell sample. (jci.org)
  • In contrast, T cell specimens usually rearranged both alleles of T beta (15 of 18), the rearrangement could be confirmed with a second restriction enzyme (17 of 18), both alleles of the first constant region gene segment of T beta always underwent either rearrangement or deletion, and the T gamma gene was also rearranged or deleted (17 of 18). (jci.org)
  • We conclude that ordered rearrangement of the T cell receptor is a rare event in B cell lymphoma and B-CLL. (jci.org)
  • We studied the configuration and expression of the gene encoding the beta chain of the T cell receptor (TCR beta) in cell lines and primary tumor cells infected by the human T cell leukemia/lymphoma (lymphotrophic) virus type I (HTLV-I). Most of the cell lines and all the primary tumor cells showed rearrangement of the TCR beta gene, and in each case the rearrangement was distinct. (rupress.org)
  • The majority of cases examined were clonal with respect to a particular TCR beta gene rearrangement. (rupress.org)
  • This suggests that the chromosomal abnormality, and possibly infection by HTLV-I, occurred before TCR beta gene rearrangement. (rupress.org)
  • The heterodimeric T-cell surface receptors, either alpha/beta (90% -95% of T cells) or gamma/delta (5% - 10% of T cells) are produced following somatic rearrangement of the T-cell receptor (TCR) genes (alpha, beta, delta, and gamma). (ohsu.edu)
  • In conjunction with morphologic evaluation of lymph nodes, bone marrow and other tissue types the detection of a clonal T-cell gene rearrangement by polymerase chain reaction (PCR) can be used to aid a diagnosis of malignant T-cell lymphoma. (ohsu.edu)
  • Loss of IL-7R function is also associated with the loss of a T-cell receptor (TCR) rearrangement. (medscape.com)
  • Scholars@Duke publication: Regulation of T cell receptor-alpha gene recombination by transcription. (duke.edu)
  • However, altered regulation of NF-κB expression and activity in TNF-treated cells may contribute to poor inducibility of IL-2 through effects on stimulus-induced changes in chromatin conformation across pIL-2. (biomedcentral.com)
  • Tissue distribution and regulation of SR-BI indicate that it may function both as a scavenger receptor and as a receptor for HDL-cholesterol. (gu.se)
  • They are known to be involved in the regulation of NK and T cell responses, mostly by homotypic interactions except for SLAMF2 (CD48) and SLAMF4 that interact with each other [ 10 ]. (nature.com)
  • The latter findings point to the participation of Nogo-A/NgR1 signaling in the regulation of other aspects of growth, such as tissue expansion or turnover by cell proliferation. (jneurosci.org)
  • Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors and commonly play an important role in the regulation of lipid homeostasis. (biomedcentral.com)
  • Epigallocatechin gallate (EGCG), an inhibitor of STAT1-mediated signaling, and AG490, a Janus kinase (JAK) inhibitor, impaired P2X 4 -receptor mRNA up-regulation by IFN-γ. (elsevierpure.com)
  • These results indicate that INF-γ selectively increases P2X 4 -receptor gene expression, leading to an up-regulation of purinergic signaling in vascular endothelial cells. (elsevierpure.com)
  • The epidermal growth factor receptor (EGFR) is frequently overexpressed in non-small-cell lung carcinoma (NSCLC), and EGFR inhibitors are promising new therapeutic agents. (nih.gov)
  • Activating mutations in downstream genes of the epidermal growth factor receptor (EGFR) pathway may cause anti-EGFR resistance in patients with colorectal cancers. (ecologicalsgardens.com)
  • Komatsu, T., Moriya, N. and Shiohara, T. (1996) T cell receptor (TCR) repertoire and function of human epidermal T cells: Restricted TCR V alpha-V beta genes are utilized by T cells residing in the lesional epidermis in fixed drug eruption. (scirp.org)
  • This ulcerated nodule of localized primary cutaneous CD30+ anaplastic large cell lymphoma is relatively nonspecific in appearance. (medscape.com)
  • GCs, such as for example dexamethasone (dex), induce cell loss of life through the GC receptor (GR), a ligand-activated transcription aspect whose transcriptional activity is necessary for GC cytotoxicity.1 GR regulates gene expression by binding DNA and nucleating the set up of regulatory cofactors. (rawveronica.com)
  • Inhibition of phosphatidylinositol 3-kinase (PI3K), a linchpin in the pre-B-cell receptor and interleukin 7 receptor signaling pathways essential to B-cell advancement (with CAL-101 [idelalisib]), interrupts a double-negative opinions loop, improving GC-regulated transcription to synergistically destroy even extremely resistant B-ALL with varied hereditary backgrounds. (rawveronica.com)
  • A novel pathogenic mutation on the interleukin-7 receptor has been described in a newborn. (medscape.com)
  • Whole cell lysates were probed for NF-κB and IκB family proteins by immunoblot. (biomedcentral.com)
  • They are specialized integral membrane proteins that allow communication between the cell and the extracellular space. (wikipedia.org)
  • Many membrane receptors are transmembrane proteins. (wikipedia.org)
  • The rotation alters which parts of the receptor are exposed on the intracellular side of the membrane, altering how the receptor can interact with other proteins within the cell. (wikipedia.org)
  • Two most abundant classes of transmembrane receptors are GPCR and single-pass transmembrane proteins. (wikipedia.org)
  • Intracellular signaling proteins: these pass the signal to the organelles of the cell. (wikipedia.org)
  • Target proteins: the conformations or other properties of the target proteins are altered when a signaling pathway is active and changes the behavior of the cell. (wikipedia.org)
  • This cell-based gene-vector approach is ideal for delivering proteins to the CNS and has particular relevance to the control of inflammatory CNS disease. (ox.ac.uk)
  • Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cells. (nature.com)
  • Multiplexed quantification of proteins and transcripts in single cells. (nature.com)
  • OxLDL also induced a cellular stress response, with increased expression of genes encoding heat shock proteins, metallothioneins and enzymes in the glutathione and the thioredoxin system. (gu.se)
  • The finding that pancreatic cancers also have KRAS mutations led the research group to explore whether T-cell receptor gene therapy that targets mutant KRAS proteins (neoantigens), could provide a positive therapeutic response for patients with pancreatic cancer as well. (cytologicsbio.com)
  • These findings suggest that, in C6 glioma cells, c-fos mRNA induction induced by EAAs is not mediated by phosphorylation of ERK and CREB proteins. (kyobobook.co.kr)
  • 2013) The molecular basis for Mucosal-Associated Invariant T cell recognition of MR1 proteins. (scirp.org)
  • When analysing separately subgroups, according to the oestrogen receptor (ER) status both approaches could predict clinical outcome more easily for the ER-positive than for the ER-negative cohort. (lu.se)
  • Lymphocyte function should be assessed by measuring responses to phytohemagglutinin, a nonspecific stimulant of T-cell proliferation, concanavalin A directed at T-cell proliferation, and pokeweed mitogen directed at T-cell and B-cell proliferation. (medscape.com)
  • This process is vital to proliferation of T-cells in normal immune function, but can be exploited to aid in the distinction between reactive (benign) versus neoplastic processes of T-cell proliferation. (ohsu.edu)
  • A reactive, benign T-cell proliferation is characterized by polyclonal expansion of T-cells whereas a malignant process is characterized by clonal expansion of one T-cell population. (ohsu.edu)
  • Overexpression of SLAMF1 or SLAMF7 in IGHV mutated CLL cell models resulted in reduced proliferation and impaired responses to BCR ligation, whereas the knockout of both receptors showed opposing effects and increased sensitivity toward inhibition of components of the BCR pathway. (nature.com)
  • B cell receptor (BCR) signaling plays a critical role in driving proliferation and survival of the malignant clone in chronic lymphocytic leukemia (CLL), supported by the clinical activity of inhibitors targeted toward BCR-associated kinases [ 1 ]. (nature.com)
  • Comparing HESC controls with HESCs deficient in SRC-3, gene enrichment analysis of the differentially expressed gene set revealed an overrepresentation of genes involved in chromatin remodeling, cell proliferation/motility, and programmed cell death. (frontiersin.org)
  • Sex steroid receptors (SSRs), which are involved in BC etiology and progression, promote BCSC proliferation, dedifferentiation and migration. (wjgnet.com)
  • Combined with simple proliferative responses, the identified nucleotides are also shown to have functional consequences by inducing T cell proliferation for CD4/V β 20-1 + T cells, while failing to induce proliferation in other T cell isolates. (scirp.org)
  • In vivo, poly(I:C) treatment increased intratumoral chemokine expression, NK-cell activation and tumor infiltration, and proliferation of tumor-infiltrating T and NK cells. (nih.gov)
  • Proliferation of tumor parenchyma cells was decreased. (nih.gov)
  • These molecular defects interfere with lymphocyte development and function, blocking the differentiation and proliferation of T cells and, in some types, of B cells and NK cells. (medscape.com)
  • In X-linked SCID, loss of IL-2 receptor (IL-2R) function leads to the loss of a lymphocyte proliferation signal. (medscape.com)
  • To characterize the biosafety profile of the CAR T cell products, we did the most comprehensive genomic insertion site analysis performed so far in T cells modified with SB. (uni-wuerzburg.de)
  • For T-cell receptor gamma targets, extracted genomic DNA from blood, lymph node, bone marrow, or other tissue types are PCR amplified and subjected to next generation sequencing (NGS) library preparation with a combination of heterogeneous TCR-gamma V(variable) region and J-(joining) region primers to generate TCR-gamma receptor VJ-rearranged templates, which are then subjected to next-generation sequencing on a MiSeq instrument. (ohsu.edu)
  • Here we show that blockade of transcriptional elongation through the mouse T cell receptor-alpha (Tcra) locus suppressed V(alpha)-to-J(alpha) recombination and chromatin remodeling of J(alpha) segments. (duke.edu)
  • An increase in the threshold cycle (C T ) of a target sequence PCR product from activated cells was indicative of stimulation-induced chromatin remodelling. (biomedcentral.com)
  • Rao S, Gerondakis S, Woltring D, Shannon MF: c-Rel is required for chromatin remodeling across the IL-2 gene promoter. (biomedcentral.com)
  • Recent technological advances have enabled massively parallel chromatin profiling with scATAC-seq (single-cell assay for transposase accessible chromatin by sequencing). (nature.com)
  • Additional strategies Chromatin immunoprecipitation accompanied by deep sequencing (ChIP-seq),1 viral planning,1 brief hairpin RNA (shRNA) testing,2 cloning of specific shRNAs and knockdown,6 quantitative polymerase string reaction,7 traditional western blotting,8 cell viability,9 and patient-derived xenograft versions10,11 had been performed mainly as previously referred to with additional information offered in supplemental Strategies (on the web page). (rawveronica.com)
  • Bharati J, Dangi SS, Mishra SR, Chouhan VS, Verma V, Shankar O, Bharti MK, Paul A, Mahato DK, Rajesh G (2017) Expression analysis of Toll like receptors and interleukins in Tharparkar cattle during acclimation to heat stress exposure. (malque.pub)
  • Isomäki P, Panesar M, Annenkov A, Clark JM, Foxwell BM, Chernajovsky Y, Cope AP: Prolonged exposure of T cells to TNF down-regulates TCR zeta and expression of the TCR/CD3 complex at the cell surface. (biomedcentral.com)
  • Bradley is investigating the process by which the androgen receptor regulates various genes. (healthresearchbc.ca)
  • A fibroblast growth factor receptor that regulates CHONDROCYTE growth and CELL DIFFERENTIATION. (bvsalud.org)
  • Drugs and drug-like molecules can modulate the function of mucosal-associated invariant T cells. (expasy.org)
  • 2012) Structural insight into MR1-mediated recognition of the mucosal associated invariant T cell receptor. (scirp.org)
  • We determined the configuration of the genes for the beta (T beta) and gamma (T gamma) chains of the T cell receptor in DNA from 100 consecutive cases of B cell lymphoma and B cell chronic lymphocytic leukemia (B-CLL), and compared the findings with those in 18 T cell neoplasms. (jci.org)
  • Importantly, CD19-CAR T cells modified by MC-based SB transposition were equally effective as LV transduced CD19-CAR T cells in vitro and in a murine xenograft model (NSG/Raji-ffLuc), where a single administration of CD8+ and CD4+ CAR T cells led to complete eradication of lymphoma and memory formation of CAR T cells after lymphoma clearance. (uni-wuerzburg.de)
  • Lymphoma is a cancer that starts in cells that are part of the body's immune system. (invivoscribe.com)
  • PCR-based detection of rearranged T-cell receptor genes can be used to help establish a diagnosis of T-cell lymphoma, monitor for treatment response, and/or measure minimal residual disease (MRD). (ohsu.edu)
  • In the ovary, SR-BI was expressed in steroidogenically active theca cells in healthy follicles and in corpus luteum, where it may enhance cholesterol uptake as a substrate for steroid hormone production. (gu.se)
  • The number and frequency of NGS sequencing reads from these rearranged VJ segment are then analyzed to assess the possible presence of a predominating clonal T-cell population. (ohsu.edu)
  • We also found that SRC-3 depletion in cultured HESCs results in a significant attenuation in the induction of a wide-range of established biomarkers of decidualization, despite exposure of these cells to a deciduogenic stimulus and normal progesterone receptor expression. (frontiersin.org)
  • ER) and progesterone receptor (PgR) parameters [1]. (lu.se)
  • Human dNK cell function is differentially regulated by extrinsic cellular engagement and intrinsic activating receptors in first and second trimester pregnancy. (nih.gov)
  • The high level of gene transfer rate and efficient genome editing, superior safety profile as well as ease-of-handling and production of non-viral MC vectors and Cas9 RNP position our developed non-viral strategies to become preferred approaches in advanced cellular and gene-therapy. (uni-wuerzburg.de)
  • A single receptor may also be differently distributed at different membrane positions, depending on the sort of membrane and cellular function. (wikipedia.org)
  • We previously demonstrated the critical involvement of SRC-2 in murine embryo implantation as well as in human endometrial stromal cell (HESC) decidualization, a cellular transformation process required for trophoblast invasion and ultimately placentation. (frontiersin.org)
  • These molecular findings are supported at the cellular level by the inability of HESCs to morphologically transform from a stromal fibroblastoid cell to an epithelioid decidual cell when endogenous SRC-3 levels are markedly reduced. (frontiersin.org)
  • Hepatitis C virus (HCV) infection has impaired cellular immune response and circulating NK cells, NK subsets (in- the ability to establish chronic infection virus persistence [8]. (who.int)
  • DNA microarray analyses using these cell lines were performed with or without adding each subtype ligand and provided much important information on the PPAR target genes involved in lipid metabolism, transport, storage and other activities. (biomedcentral.com)
  • Cell surface receptors (membrane receptors, transmembrane receptors) are receptors that are embedded in the plasma membrane of cells. (wikipedia.org)
  • Transmembrane receptors are typically classified based on their tertiary (three-dimensional) structure. (wikipedia.org)
  • Two models have been proposed to explain transmembrane receptors' mechanism of action. (wikipedia.org)
  • Rotation: Ligand binding to the extracellular part of the receptor induces a rotation (conformational change) of part of the receptor's transmembrane helices. (wikipedia.org)
  • Transmembrane receptors in plasma membrane can usually be divided into three parts. (wikipedia.org)
  • In other receptors, the transmembrane domains undergo a conformational change upon binding, which affects intracellular conditions. (wikipedia.org)
  • In some receptors, such as members of the 7TM superfamily, the transmembrane domain includes a ligand binding pocket. (wikipedia.org)
  • Signaling Lymphocytic Activation Molecule Family (SLAMF) receptors are a group of nine type I transmembrane receptors that are mainly expressed on a variety of immune cells. (nature.com)
  • human neuronal tissue.3C5 All three TRK receptors include an extracellular ligand-binding domain, a transmembrane region and an intracellular adenosine triphosphate-binding domain.2 6 TRK receptors are activated when neurotrophin ligands bind towards the extracellular site from the receptor (figure 1A). (researchtoactionforum.org)
  • SCID results from mutations in any of more than 15 known genes. (medscape.com)
  • The advances in genetic engineering have enabled us to confer T cells new desired functions or delete their specific undesired endogenous properties for improving their antitumor function. (uni-wuerzburg.de)
  • One example is adoptive cell therapy with T cells that were genetically modified with gamma-retroviral and lentiviral (LV) delivery vectors to express a CD19-specific chimeric The advances in genetic engineering have enabled us to confer T cells new desired functions or delete their specific undesired endogenous properties for improving their antitumor function. (uni-wuerzburg.de)
  • In conclusion, we provide a novel platform for virus-free genetic engineering of CAR T cells that can be broadly applied in T-cell cancer therapy. (uni-wuerzburg.de)
  • These findings, combined with available genetic evidence from Drosophila, support the hypothesis that Ptc is a receptor for Shh, and that vSmo could be a signalling component that is linked to Ptc. (nih.gov)
  • Explore the normal functions of human genes and the health implications of genetic changes. (medlineplus.gov)
  • Nam, A. S., Chaligne, R. & Landau, D. A. Integrating genetic and non-genetic determinants of cancer evolution by single-cell multi-omics. (nature.com)
  • With the advances in BMT and gene therapy, patients now have a better likelihood of developing a functional immune system in a previously lethal genetic disease. (medscape.com)
  • Detailed molecular analyzes showed that SLAMF1 and SLAMF7 receptors mediate their BCR pathway antagonistic effects via recruitment of prohibitin-2 (PHB2) thereby impairing its role in signal transduction downstream the IGHV-mutant IgM-BCR. (nature.com)
  • Whole blood cells, aorta and lung were harvested for global gene expression analysis with subsequent Ingenuity Pathway Analysis and confirmatory qRT-PCR. (cdc.gov)
  • Ingenuity Pathway Evaluation software program (Qiagen) was utilized to execute pathway and gene ontology evaluation of differentially controlled genes. (rawveronica.com)
  • How does tumour necrosis factor uncouple T-cell receptor-induced IL-2 gene expression in murine T cells? (biomedcentral.com)
  • Cells were grown in the presence or absence of 2.5 ng/ml murine TNF for 8 days. (biomedcentral.com)
  • Chronic culture of murine T cells in TNF does not alter the stability of IL-2 mRNA induced via TCR, or by PMA and ionomycin, in those cells. (biomedcentral.com)
  • In a murine relapsing experimental allergic encephalomyelitis (EAE) model, gene therapy to block TNF was investigated with the use of a retroviral dimeric p75 TNF receptor (dTNFR) construct. (ox.ac.uk)
  • Consequently, there is a strong desire to make CAR T cell therapy scalable and globally available to patients. (uni-wuerzburg.de)
  • this response aids in defining the type of SCID but also is relevant to determining the need for immunosuppressive therapy in preparation for stem cell reconstitution. (medscape.com)
  • Gene therapy for chronic relapsing experimental allergic encephalomyelitis using cells expressing a novel soluble p75 dimeric TNF receptor. (ox.ac.uk)
  • Although the patient experienced short-lived toxic events associated with the preconditioning therapy that was provided days before the T-cell infusion, no adverse effects were seen with the engineered T cells. (cytologicsbio.com)
  • The research data obtained in these one-patient clinical trials warrant additional studies with more participants to determine the efficacy and safety of neoantigen T-cell receptor gene therapy for pancreatic and other mutant KRAS-expressing cancers. (cytologicsbio.com)
  • In recent exploratory regional therapy alone or by the addition of systemical studies, markers from microarray gene expression medical therapy with increased side effects and costs. (lu.se)
  • Cell lines infected by HTLV-I express levels of TCR beta mRNA similar to PHA stimulated lymphocytes, suggesting that this gene is not transcriptionally activated as a result of infection by HTLV-I. Cloned T cells of known antigen specificity that are infected by HTLV-I in vitro show impairment of immune function, including loss of antigen-specific responsiveness and the acquisition of alloreactivity. (rupress.org)
  • Engler, O.B., Strasser, I., Naisbitt, D.J., Cerny, A. and Pichler, W.J. (2004) A chemically inert drug can stimulate T cells in vitro by their T cell receptor in non-sensitised individuals. (scirp.org)
  • We have previously described an in vitro model using human SCLC cell lines that express POMC and are resistant to glucocorticoids. (manchester.ac.uk)
  • HCC cell death, and natural killer (NK) cell activation and cytotoxicity were assessed in vitro after treatment with the TLR3 ligand poly(I:C). The effect of TLR3 on the tumor parenchyma and infiltrating immune cells was investigated in a spontaneous liver tumor mouse model and a transplanted tumor mouse model (n = 3-9 mice per group). (nih.gov)
  • OxLDL increased expression of genes involved in uptake of oxLDL, storage of lipids and cholesterol efflux whereas expression of genes involved in cholesterol synthesis and LDL-uptake was decreased. (gu.se)
  • T cell receptor gene studies allow B and T cell lymphomas to be distinguished from each other and from common acute lymphoblastic leukemia antigen-positive non-T, non-B acute lymphoblastic leukemia. (jci.org)
  • In addition, pulmonary expression of interferon alpha and beta and Irf7 specific pattern recognition receptors (PRR) and signaling molecules (Ddx58, Ifih1, Dhx58, ISGF3) were induced, an effect that showed specificity when compared to other inflammatory exposures. (cdc.gov)
  • 2007) Structural basis of T-cell specificity and activation by the bacterial superantigen TSST-1. (scirp.org)
  • In this study, we further characterize the properties of these T cells by analyzing their epitope specificity and T cell receptor (TCR) gene usage. (uni-luebeck.de)
  • Using microarrays, a technology that allows thousands of genes to be examined in a single experiment, she hopes to identify genes that are regulated by the androgen receptor and other genes that progress to androgen-independence. (healthresearchbc.ca)
  • To identify genes, signaling pathways and networks that are controlled by SRC-3 and potentially important for hormone-dependent decidualization, we performed RNA-sequencing on HESCs in which SRC-3 levels were significantly reduced at the time of administering the deciduogenic stimulus. (frontiersin.org)
  • IFN-γ did not affect P2X 4 -receptor mRNA stability, but increased P2X 4 -receptor gene transcription in a cycloheximide- insensitive manner. (elsevierpure.com)
  • We have found that, although chronic TNF attenuates TCRζ expression and TCR-proximal signalling, IL-2 expression is suppressed regardless of whether cells are stimulated via the TCR, or with phorbol ester and calcium ionophore to activate the MAPK and NFAT pathways directly. (biomedcentral.com)
  • two with scant tumor cells one referral case with no tumor cells on the accompanying hematoxylin & eosin (H&E) slide and one with the assay being successfully conducted in the primary tumor. (ecologicalsgardens.com)
  • Configuration and expression of the T cell receptor beta chain gene in human T-lymphotrophic virus I-infected cells. (rupress.org)
  • Locus controlling Bordetella pertussis-induced histamine sensitization (Bphs), an autoimmune disease-susceptibility gene, maps distal to T-cell receptor beta-chain gene on mouse chromosome 6. (ox.ac.uk)
  • Levels of expression of the TCR beta gene at the mRNA level and surface expression of the T3 complex were also not significantly altered, suggesting that changes in immune function cannot be attributed to quantitative changes in the TCR molecule. (rupress.org)
  • Each cell membrane can have several kinds of membrane receptors, with varying surface distributions. (wikipedia.org)
  • Receptors are often clustered on the membrane surface, rather than evenly distributed. (wikipedia.org)
  • The study was conducted to establish the differences in the expression pattern of different cell surface toll-like-receptors ( TLRs) between indigenous Osmanabadi and Salem Black goats subjected to summer season induced heat stress. (malque.pub)
  • The higher expression of most of the cell surface TLRs in the SBHS group as compared to OHS group indicates the superior resilient capacity of Salem Black goats to maintain immune status even during exposure to adverse environmental condition. (malque.pub)
  • T-cell surface glycoprotein CD3 delta chain precursor (T-cell receptor T3 delta chain). (lu.se)
  • In the mouse brain, we found that the plasticity inhibitors Nogo-A and Nogo receptor 1 (NgR1) are differentially expressed in the SVZ-OB system, in which Nogo-A identifies immature neuroblasts and NgR1 germinal astrocytes. (jneurosci.org)
  • NKp80 Defines a Critical Step during Human Natural Killer Cell Development. (nih.gov)
  • Identification of NKp80, a novel triggering molecule expressed by human NK cells. (nih.gov)
  • Due to their efficient gene delivery, viral vectors have been successfully used in T-cell engineering to provide gene transfer medicinal products for the treatment of human disease. (uni-wuerzburg.de)
  • It was previously unknown how variable human TCR genes are", says Gunilla Karlsson Hedestam , professor at the department of microbiology, tumor and cell biology at Karolinska Institutet and the study's lead author. (ki.se)
  • Fig. 3: Dissection of native human hematopoiesis with multimodal cell state inference and mtDNA-based lineage tracing. (nature.com)
  • Fig. 6: Multiplexed CRISPR perturbations with ASAP-seq in primary human T cells. (nature.com)
  • The aim of this thesis was to examine the changes in gene expression during foam cell formation and to study the HDL-receptor scavenger receptor class B type I (SR-BI), which may be of importance for reverse cholesterol transport.To search for genes that may be of importance for foam cell formation, expression profiling of human macrophages exposed to oxLDL was performed. (gu.se)
  • The rat and human SR-BI genes were mapped to rat and human chromosomes 12. (gu.se)
  • These mRNAs were detected in human leukemia cells, CMK, K562 and HEL, which have megakaryocytic phenotype or the potency to differentiate into megakaryocytic lineage. (elsevierpure.com)
  • To identify human PPARs-responsive genes, we established tetracycline-regulated human hepatoblastoma cell lines that can be induced to express each human PPAR and investigated the gene expression profiles of these cells. (biomedcentral.com)
  • From the detailed analyses by reporter assays, the EMSAs, and ChIP assays, we determined the functional PPRE of the human adrp gene. (biomedcentral.com)
  • The results suggest that these cell lines are important tools used to identify the human PPARs-responsive genes. (biomedcentral.com)
  • Treatment of human umbilical vein endothelial cells (HUVECs) with IFN-γ for 24 h resulted in an enhancement of the ATP-induced increase in intracellular Ca 2+ concentration ([Ca 2+ ] i ) without affecting the UTP-induced one. (elsevierpure.com)
  • 1998) Comparative sequence analysis of the human T cell receptor beta chain in juvenile rheumatoid arthritis and juvenile spondylarthropathies: Evidence for antigenic selection of T cells in the synovium. (scirp.org)
  • Some human small cell lung carcinomas (SCLC) secrete proopiomelanocortin (POMC) derived peptides, but in contrast to the pituitary, glucocorticoids fail to inhibit this hormone production. (manchester.ac.uk)
  • The configuration of the TCR beta gene in primary tumor cells infected by HTLV-I was compared with that in the derived cell lines. (rupress.org)
  • In all pairs examined, the configuration in the primary tumor cells was different from that in the cell lines, strongly suggesting that the cells that grow in culture are not the original neoplastic cells. (rupress.org)
  • With the help of TCRs, the cells recognize foreign invaders and tumor cells. (ki.se)
  • Immunotherapy for patients with cancer involves the use of immune cells, often the patient's own cells, to develop a treatment to target tumor cells. (cytologicsbio.com)
  • ALCL was recognized in 1985, when tumor cells consistently demonstrated labeling by the monoclonal antibody Ki-1, a marker later shown to recognize the CD30 antigen. (medscape.com)
  • These results suggest that SR-BI may have both scavenger receptor and HDL-receptor function in the ovary. (gu.se)
  • Peripheral blood mononuclear cells (PBMCs) were collected from the patient as the first step in creating the immunotherapy. (cytologicsbio.com)
  • Clark JM, Annenkov AE, Panesar M, Isomäki P, Chernajovsky Y, Cope AP: T cell receptor zeta reconstitution fails to restore responses of T cells rendered hyporesponsive by tumor necrosis factor alpha. (biomedcentral.com)
  • SCID is considered a pediatric emergency because survival depends on expeditious stem cell reconstitution, usually by bone marrow transplantation (BMT). (medscape.com)
  • T-cells that are part of our immune system are central in the protection against infections and cancer. (ki.se)
  • As these genes are among the most variable in our genome, the results also provide new information about how our immune system has developed over the course of history, says Martin Corcoran. (ki.se)
  • Normal plasma cells are found in the bone marrow and are an important part of the immune system. (invivoscribe.com)
  • Together, our data indicate that SLAMF receptors are important modulators of the BCR signaling axis and may improve immune control in CLL by interference with NK cells. (nature.com)
  • In the work presented here, we provide compelling data that SLAMF1 and SLAMF7 receptors may not only enhance immune control of CLL but also negatively regulate BCR signaling and thereby impact sensitivity towards BTK inhibition in the substantial fraction of patients with SLAMF1 or SLAMF7 expressing M-CLL. (nature.com)
  • Studies sug- terized by flow cytometry using anti between the immune response and the gest that HCV inhibits receptor genes in CD3, CD56 and CD16 monoclonal virus replication rate [5] and play a cru- the activation of NK cells [20], and the antibodies. (who.int)
  • In order to study the possible role over Ficoll-Hypaque (density 1.077 production, and are the key players in that NK cells might play in the patho- g/L) (Amersham Biosciences) and immune responses to viruses [7]. (who.int)
  • They act in cell signaling by receiving (binding to) extracellular molecules. (wikipedia.org)
  • The extracellular domain is just externally from the cell or organelle. (wikipedia.org)
  • For example, a neurotransmitter, hormone, or atomic ions may each bind to the extracellular domain as a ligand coupled to receptor. (wikipedia.org)
  • Signal transduction through membrane receptors requires four parts: Extracellular signaling molecule: an extracellular signaling molecule is produced by one cell and is at least capable of traveling to neighboring cells. (wikipedia.org)
  • The increased Ca 2+ response to ATP in IFN-γ-treated cells was dependent on the extracellular Ca 2+ , and was not inhibited by the phospholipase C inhibitor U73122. (elsevierpure.com)
  • Molecular characterization of two novel alternative spliced variants of the KLRF1 gene and subcellular distribution of KLRF1 isoforms. (nih.gov)
  • The methods described in the study provide new opportunities, not the least in the cancer field where T-cells are central to several promising forms of immunotherapy", says Gunilla Karlsson Hedestam. (ki.se)
  • Schier, A. F. Single-cell biology: beyond the sum of its parts. (nature.com)
  • Since SLAMF receptors play a role in NK cell biology, we reasoned that these receptors may impact NK cell-mediated CLL immunity. (nature.com)
  • they react with the receptor to induce changes in the metabolism and activity of a cell. (wikipedia.org)
  • Overall, our data suggest that polymorphisms in the host NKG2D and NKG2A genes have a protective role in SARS-CoV-2 infection, although the functional impact of these polymorphisms on control of SARS-CoV-2 infection remains unknown. (bvsalud.org)
  • gene polymorphisms and symptomatic differences in patients with narcolepsy type 1. (cdc.gov)
  • abstract = "K- sam bek, N- sam flg and FGFR3 sam3 establish gene family of the receptors for heparin-binding growth factors (HBGFs) or FGFs. (elsevierpure.com)
  • This failure of the GR explains the resistance of the POMC gene to glucocorticoid inhibition and may have implications for cell growth in SCLC. (manchester.ac.uk)
  • as genes that impact the level of sensitivity of B-ALL cells to dex. (rawveronica.com)
  • Draw lymphocyte markers at the same time as the CBC to obtain percentages and absolute counts of CD3 + T cells, CD4 + T cells, CD8 + T cells, CD19 + B cells, and natural killer (NK) cell markers (CD16 and CD56). (medscape.com)
  • The percentage of CD56(bright) cells was significantly higher than the control group ( P = 0.04). (who.int)
  • While the percentages of CD56 (dim) cells and their CD16 expression were lower in the chronic group, this was not statistically significant. (who.int)
  • The frequency of CD3+CD56- T cells was significantly lower in both the chronic and resolved groups compared to the control group ( P = 0.04). (who.int)
  • The elimination or persistence (NKRs) and mediate functions of both cells and CD56+NT cells was charac- of the infection depends on a balance T cells and NK cells [13]. (who.int)
  • Chen L, Yu J (2016) Modulation of Toll-like receptor signaling in innate immunity by natural products. (malque.pub)
  • NK cells, a component of innate immunity, are variably affected. (medscape.com)
  • A complete absence of T-cell function by mitogen tests can occur in association with a normal lymphocyte count for age in some forms of SCID, including X-linked SCID (XL-SCID), in which all the lymphocytes are B cells. (medscape.com)