• inactive
  • Mutations in some of the codons of the N-terminal 60aa or C-terminal 100aa results in an inactive, dimeric β-galactosidase. (openwetware.org)
  • When B-galactosidase is separated into 2 fragments, it has the unique property of regaining enzymatic activity upon the rejoining of the inactive fragments. (wikipedia.org)
  • coli
  • The 1,024 amino acids of E. coli β-galactosidase were first sequenced in 1970, and its structure determined twenty-four years later in 1994. (wikipedia.org)
  • amino
  • A mutant with a deletion of everything past residue 60 (i.e. it expresses only the first 60 N-terminal amino acids) does not produce any active β-galactosidase. (openwetware.org)
  • include
  • If anyone has a better reference comparing results from a Miller assay of alpha-complementated β-galactosidase with wildtype, please include it here. (openwetware.org)
  • blue
  • They proposed a cytochemical assay based on production of a blue-dyed precipitate that results from the cleavage of the chromogenic substrate X-Gal, which stains blue when cleaved by galactosidase. (wikipedia.org)