• chemists
  • They are useful to chemists as dyes or markers, and can be used for such applications as medical and biological imaging. (umn.edu)
  • The novel technique could allow chemists to synthesize dyes in hundreds of different colors. (photonics.com)
  • The physicists and chemists at GreenSun, led by Renata Reisfeld, coat the glass with metallic nanoparticles and dyes to cause the sunlight falling on the glass to diffuse sideways toward the edges where the silicon strips turn it into electricity. (wikipedia.org)
  • protein
  • However, current monitoring methods fall short, said the study's first author Evan W. Miller, a post-doctoral researcher in the lab of Roger Tsien, PhD, Howard Hughes Medical Institute investigator, UC San Diego professor of pharmacology, chemistry and biochemistry and 2008 Nobel Prize co-winner in chemistry for his work on green fluorescent protein. (ucsd.edu)
  • These unique dyes are used in a variety of NIR imaging applications including Western blotting , plate-based assays , protein arrays, tissue section imaging, probe development , and small animal imaging . (licor.com)
  • These techniques are limited by their requirement for both highly purified proteins and solvatochromic dyes, prompting the need for more robust high-throughput technologies that can be used with crude protein samples. (wikipedia.org)
  • When the protein unfolds, the exposed hydrophobic surfaces bind the dye, resulting in an increase in fluorescence by excluding water. (wikipedia.org)
  • Some of the values are used to estimate the extent of success in attempts to conjugate the dye to a protein. (wikipedia.org)
  • Wayback Machine
  • Fluorescence microscope FLEX mission Fluorescent green pig Float tracking An educational website about karst and dye tracing, by Crawford Hydrology Laboratory / Center for Cave and Karst Study in association with Western Kentucky University Water Tracing Dye Technical Bulletin Archived 2007-02-03 at the Wayback Machine. (wikipedia.org)
  • BODIPY Dye Series Archived 2008-02-26 at the Wayback Machine. (wikipedia.org)
  • light
  • When the dyed cells are exposed to light, neuronal firing causes the dye momentarily to glow more brightly, a flash that can be captured with a high-speed camera. (ucsd.edu)
  • Fluorescent chromophores are compounds that can emit light under certain circumstances. (umn.edu)
  • Magnaflux® Fluorescent Dye Penetrant Kit comes with dyes, a UV light, and other accessories to help you find the tiniest cracks in just about any metal surface. (grainger.com)
  • As the spatial resolution of STED imaging correlates with an increase in STED light intensity, photodegradation of fluorescent dyes becomes a serious issue. (innovations-report.com)
  • For example, a dye that is excited by blue light at about 490 nanometers emits a green light in a range of 510-530 nanometers. (berkeley.edu)
  • A mixture of dyes is used to capture and absorb a wide spectrum of available light. (wikipedia.org)
  • stable
  • Dansyl chloride or 5-(DimethylAmino)Naphthalene-1-SulfonYL chloride is a reagent that reacts with primary amino groups in both aliphatic and aromatic amines to produce stable blue- or blue-green-fluorescent sulfonamide adducts. (wikipedia.org)
  • Also, due to this stable linkage, once incorporated within cells the dye is not transferred to adjacent cells. (wikipedia.org)
  • subsequent
  • Subsequent studies revealed that the dye can be used to monitor lymphocyte proliferation, both in vitro and in vivo, due to the progressive halving of CFSE fluorescence within daughter cells following each cell division. (wikipedia.org)
  • emission
  • Cy5 become very popular to replace far red fluorescent dyes, because of its high extinction coefficient (as small as 1 nanomol can be detected in gel electrophoresis by naked eye) and of its fluorophore emission maximum in red region, where many CCD detectors have maximum sensitivity, and biological objects have low background. (wikipedia.org)
  • available
  • Cyanines dyes are available with different modifications such as methyl, ethyl or butyl substituent, carboxyl, acetylmethoxy, and sulfo groups (conferring hydrophilicity). (wikipedia.org)
  • Sulfo-Cyanine dyes bear classically one or two Sulfo groups, rendering the Cy dye water-soluble, but tri- and quadri-sulfonated forms are available for even higher hydrosolubility. (wikipedia.org)
  • calcium
  • Later, we devised indirect solutions such as calcium imaging or dyes that gave big but slow responses to voltage. (ucsd.edu)
  • different
  • Scientists used to concoct different dyes mostly by trial and error, Lavis said. (photonics.com)
  • Each dye has significant performance factors that distinguish it in different settings. (wikipedia.org)
  • In case both primers are labeled, different fluorescent dyes are required. (wikipedia.org)
  • Another modification which is sometimes used is to fluorescently label the reverse primer as well using a different dye, again resulting in two parallel profiles per sample each resolving a different number of variants. (wikipedia.org)
  • added using s
  • However, dyes for AC systems will have to be added using special tools to prevent the further loss of refrigerant when looking for a leak. (acdelco.com)
  • voltage
  • Leech neurons stained with voltage-sensitive dye. (ucsd.edu)
  • One of the tradeoffs with using voltage-sensing dyes in the past is that when they were reasonably sensitive to voltage changes, they were slow compared to the actual physiological events," said Miller. (ucsd.edu)
  • blue
  • Nile blue is a fluorescent dye. (wikipedia.org)
  • Nile Blue and related naphthoxazinium dyes can be prepared by acid-catalyzed condensation of either 5-(dialkylamino)-2-nitrosophenols with 1-naphthylamine, 3-(dialkylamino)phenols with N-alkylated 4-nitroso-1-naphtylamines, or N,N-dialkyl-1,4-phenylenediamines with 4-(dialkylamino)-1,2-naphthoquinones. (wikipedia.org)
  • bind
  • Swager, who is head of the chemistry department, and colleagues developed the new dye, called NIAD-4, through a targeted design process based on a set of specific requirements, including the ability to enter the brain rapidly upon injection, bind to amyloid plaques, absorb and fluoresce radiation in the right spectral range, and provide sharp contrast between the plaques and the surrounding tissue. (medgadget.com)
  • Depending
  • Depending on the chemical structure of the dye, the particles are filtered out of the blood either via the kidney tissue or via liver cells," said professor Dr. Michael Bauer of the Jena University Hospital Center for Sepsis Control and Care. (photonics.com)
  • sunlight
  • Having absorbed the sunlight, the fluorescent dyes then re-radiate it. (wikipedia.org)
  • Sunlight scatters its energy, but the dye/nanoparticle mix in the GreenSun process delivers plasmons and photons of the right energy to knock electrons free more efficiently. (wikipedia.org)
  • cells
  • The new method employs dyes that penetrate only the membrane of neurons, either in in vitro cells cultured with the dye or, for this study, taken up by neurons in a living leech model. (ucsd.edu)
  • But the dyes also play another key function: Because they mimic a cellular transporter of liver epithelial cells, the liver absorbs them readily. (photonics.com)
  • Due to this covalent coupling reaction fluorescent CFSE can be retained within cells for extremely long periods. (wikipedia.org)
  • Furthermore, the use of CFSE has extended beyond the immune system, with the dye being used to monitor the proliferation of many other cell types such as smooth muscle cells, fibroblasts, hematopoietic stem cells and even bacteria. (wikipedia.org)
  • These dyes aggregate in the tumor cells, especially in the lipid membranes, and/or are sequestered and concentrated in subcellular organelles. (wikipedia.org)
  • The dyes are membrane-impermeant and can be loaded into cells by microinjection or scrape loading. (wikipedia.org)
  • applications
  • Biological applications include comparative genomic hybridization and gene chips, which are used in transcriptomics, and various studies in proteomics such as RNA localization, molecular interaction studies by fluorescence energy transfer (FRET) and fluorescent immunoassays. (wikipedia.org)
  • It is used in in vivo imaging applications, as well as the Cy7.5 dye. (wikipedia.org)
  • studies
  • Thus CFSE represents an extremely valuable fluorescent dye for immunological studies, allowing lymphocyte proliferation, migration and positioning to be simultaneously monitored. (wikipedia.org)
  • work
  • Richard Paul "Dick" Haugland (July 17, 1943 - October 5, 2016) was an American scientist noted for his work in researching and commercializing fluorescent dyes. (wikipedia.org)
  • Analysis
  • Sewer and stormwater drainage analysis Dye tracing may be used for the analysis of blood circulation within various parts of the human or animal body. (wikipedia.org)
  • For example, fluorescent angiography, a technique of analysis of circulation in retina is used for diagnosing various eye diseases. (wikipedia.org)
  • chemistry
  • In a recent paper that appeared in the Journal of the American Chemical Society , Professor Victor Nemykin ( Chemistry and Biochemistry , University of Minnesota Duluth ) and his collaborators at the University of Akron (Akron, Ohio) discuss a new fluorescent chromophore, or fluorophore, called BOPHY. (umn.edu)
  • Often
  • Often fluorescent dyes are used for this purpose, especially in the following circumstances Insufficient lighting (e.g., sewers or cave waters) Precise quantitative data are required, measured by a fluorometer Very small amounts of the dye is allowed to be added (1 part per trillion may be detected). (wikipedia.org)
  • useful
  • Therefore, one dye can be particularly useful in one environment, whereas it could be inadequate another. (wikipedia.org)
  • either
  • Dye tracking may be either qualitative, i.e., the presence of particular flow and its estimate, or quantitative, when the amount of the traced dye is measured by special instruments. (wikipedia.org)