• Also, the fluorescence lifetimes of the protonated and deprotonated forms of fluorescein are approximately 3 and 4 ns, which allows for pH determination from nonintensity based measurements. (wikipedia.org)
  • However while agents such as fluorescein and aminolevulinic acid have been shown to increase the extent of resection, these fluorophores may result in the fluorescence of the surrounding edematous brain. (cns.org)
  • Cellular fluorescence in U87 cells was highest following treatment with 5ug/mL of EGF-FL, with this fluorescence being significantly greater than that achieved by 15ug/mL of fluorescein (p=0.0014). (cns.org)
  • Evaluation of fluorescein tumor specificity revealed tumor tissue to account for only 68.4% and 59.1% of total fluorescence in low and high EGFR expressing tumors, respectively. (cns.org)
  • Due to the established safety profile of fluorescein and the ease of administration, EGF-fluorescein has direct clinical applicability for use in fluorescence-guided resections and requires further study. (cns.org)
  • While the most investigated agents including fluorescein and aminolevulinic acid have been shown to increase the rates of gross total resection, these fluorophores may result in the fluorescence of the surrounding edematous brain, potentially resulting in the resection of non-tumor brain tissue. (cns.org)
  • Fluorescein derivatives are one of the most commonly used fluorescent labels for biological detection in flow cytometry and immunofluorescence due to their high absorptivity, excellent fluorescence quantum yields and good water solubility. (aatbio.com)
  • Despite its bright green fluorescence, fluorescein based-dyes and conjugates have a number of caveats that may restrict its use in certain applications, including rapid photobleaching, pH-sensitive fluorescence and susceptibility to quenching. (aatbio.com)
  • To ensure full fluorescence and patient comfort, the Fluorescein Sodium impregnated tip should be moistened before application. (petseyes.cn)
  • Fluorescein-labeled probes can be used for in situ hybridization with direct fluorescence detection and detection by ELISA using Anti-Fluorescein-AP, Fab fragments. (roche.com)
  • Human transferrin fluorescein conjugation has been tested in dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial platforms. (rockland.com)
  • Mice were intravenously administered fluorescein sodium or EGF-FL and then sacrificed. (cns.org)
  • The dye called sodium fluorescein is a toxic substance that can immediately alter the pH balance in the blood. (medicalhealthtests.com)
  • Methods: This trial was conducted at two centers : Tata Memorial Centre, Mumbai and All India Institute of Medical Sciences New Delhi in India.We examined 86 patients of early breast cancer with no palpable axillary nodes undergoing SLNB with three tracers (Sulphur colloid /Methylene blue / Sodium Fluorescein). (aacrjournals.org)
  • In this study, we have evaluated the specificity of epidermal growth factor conjugated fluorescein (EGF-FL) and have compared it to conventional fluorescein in vitro and in vivo. (cns.org)
  • EGF-FL is a highly specific fluorophore for the delineation of EGFR-expressing tumors and has comparable efficacy to conventional fluorescein in tumors with low EGFR expression. (cns.org)
  • Additionally, we have compared EGF-FL to conventional fluorescein to evaluate their relative efficacy. (cns.org)
  • We offer a broad selection of fluorescein based-dyes, substrates and conjugates, as well as a series of superior iFluor® fluorescent labeling dyes with improved characteristics optimized for cellular labeling and detection. (aatbio.com)
  • Fluorescein-DBCO is a fluorescent dye commonly used in drug research and development to label and track molecules in biological systems. (axispharm.com)
  • Preoperative iris fluorescein angiograms (IFA) and ophthalmic records of 34 diabetic patients who underwent vitrectomy were reviewed. (unboundmedicine.com)
  • Albrecht von Graefe's archive for clinical and experimental ophthalmology JO - Albrecht Von Graefes Arch Klin Exp Ophthalmol VL - 206 IS - 1 N2 - Preoperative iris fluorescein angiograms (IFA) and ophthalmic records of 34 diabetic patients who underwent vitrectomy were reviewed. (unboundmedicine.com)
  • Using this method, saturation curves for Rhodamine B, Rhodamine 560, Fluorescein, and Coumarin 153 were obtained using only one image of the saturated sample and one reference image in each case. (lu.se)
  • Many derivatives of fluorescein are known. (wikipedia.org)
  • We offer a broad selection of fluorescein products including reactive fluorescein and fluorescein derivatives for labeling antibodies, nucleic acids and other biomolecules, conjugates, indicators, and substrates for detecting enzymatic activity in cells, homogenates and solution. (aatbio.com)
  • We can supply derivatives of either of these qualities labelled with fluorescein (F-HA). (tdblabs.se)
  • Fluorescein has a pKa of 6.4, and its ionization equilibrium leads to pH-dependent absorption and emission over the range of 5 to 9. (wikipedia.org)
  • Fluorescein has an isosbestic point (equal absorption for all pH values) at 460 nm. (wikipedia.org)
  • Fluorescein injection is used to help certain parts of the eye (eg, retina, iris) become more visible during eye medical procedures. (mayoclinic.org)
  • In oligonucleotide synthesis, several phosphoramidite reagents containing protected fluorescein, e.g. 6-FAM phosphoramidite 2, are used for the preparation of fluorescein-labeled oligonucleotides. (wikipedia.org)
  • Fluorescein is a fluorophore commonly used in microscopy, in a type of dye laser as the gain medium, in forensics and serology to detect latent blood stains, and in dye tracing. (wikipedia.org)
  • Use of EGF-FL in U87 tumors revealed similar tumor specificity as fluorescein (70.9% vs. 68.4%, p=0.71). (cns.org)
  • Phosphoramidite can be used for internally labelling an oligonucleotide with the fluorescein-dT dye moiety. (biosearchtech.com)
  • The hydrolysis of the fluorescein diacetate (FDA), related to several soil hydrolases, has been utilised to estimate the potential microbial activity of soil freshly amended with a wide range of organic amendments and compared to the size and activity of soil microflora, measured by the microbial biomass C ( B C ) and CO 2 evolution, respectively. (springer.com)
  • Adam G, Duncan H (2001) Development of a sensitive and rapid method for the measurement of total microbial activity using fluorescein diacetate (FDA) in a range of soils. (springer.com)
  • Conclusions: Fluorescein can be used as a low cost and effective alternative in sentinel lymph node biopsy for carcinoma breast without the risk of radiation exposure. (aacrjournals.org)
  • This complex then reacts with fluorescein-labeled antibody. (cdc.gov)
  • What is the CPT for a fluorescein dye & wood lamp used to evaluate the entire colon for vascular/ischemic necrosis? (aapc.com)
  • Fluorescein dye disappearance test was used to evaluate tear drainage after experimental obstruction of upper, lower, neither or both canaliculi in 18 subjects by hydroxypropil cellulose rods. (nih.gov)
  • Since this study is first with use of fluorescein for sentinel node biopsy in breast, we injected fluorescein at decreasing time interval from 12 hours to 5 minutes to ascertain the most appropriate time for injection of fluorescein in first 15 patients. (aacrjournals.org)
  • Fluorescein sodium, the sodium salt of fluorescein, is used extensively as a diagnostic tool in the field of ophthalmology and optometry, where topical fluorescein is used in the diagnosis of corneal abrasions, corneal ulcers and herpetic corneal infections. (wikipedia.org)
  • This study has demonstrated targeted EGF-FL to be significantly more specific than fluorescein in EGFR-overexpressing tumors, with comparable efficacy in tumors with low EGFR expression. (cns.org)
  • 25 independent samples (14 tumors, 2 generally reflected in related patterns of fluorescein. (lu.se)
  • The color of its aqueous solutions is green by reflection and orange by transmission (its spectral properties are dependent on pH of the solution), as can be noticed in bubble levels, for example, in which fluorescein is added as a colorant to the alcohol filling the tube in order to increase the visibility of the air bubble contained within. (wikipedia.org)
  • This characteristic makes Fluorescein-DBCO a valuable tool for studying biological processes and drug interactions in real-time. (axispharm.com)
  • More concentrated solutions of fluorescein can even appear red (because under these conditions nearly all incident emission is re-absorbed by the solution). (wikipedia.org)
  • It is available as sterile single-use sachets containing lint-free paper applicators soaked in fluorescein sodium solution. (wikipedia.org)
  • ProductData": { "ID": "3.6.13.2.1.1", "ProductType": "Others", "BrandName": "Fluorescein-12-dUTP", "ProductNameAddition": "1 mM solution", "RegulatoryDisclaimer1": "For further processing only. (roche.com)
  • Oral and intravenous use of fluorescein can cause adverse reactions, including nausea, vomiting, hives, acute hypotension, anaphylaxis and related anaphylactoid reaction, causing cardiac arrest and sudden death due to anaphylactic shock. (wikipedia.org)
  • In this work, fluorescein was incorporated with an equal molar of 1-chloro-4-nitrobenzene and double potassium carbonate (molar ratio), the solvent of this reaction is dimethylacetamide (50 ml for 5 g of fluorescein). (scienceforums.net)
  • Fluorescein-12-dUTP replaces dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. (roche.com)
  • In this study we have investigated the effectiveness and safety of fluorescein in sentinel node biopsy in a cross-sectional analytical study compared to Methylene Blue and Technitium Sulphar colloid. (aacrjournals.org)
  • Fluorescein is also known as a color additive (D&C Yellow no. 7). (wikipedia.org)
  • Fluorescein helps the doctor see parts of the eye. (rxwiki.com)