FEN1PolymeraseRAD2Single strandedCryptococcusSpecificityProteinReplicationActivityStructure-specific endonucleaseApyrimidinicOkazakiInteractsPolymeraseExonuclease activitiesHelicaseGenotypesCleaveCharacterizationSubstratesSynthesisEnzymeDownstreamGeneIncisionSingle-strandInteractionsCatalyticStructuralFunction
FEN16
- Flap endonuclease 1 is an enzyme that in humans is encoded by the FEN1 gene. (wikipedia.org)
- In eukaryotes, DNA polymerase δ (Pol δ) bound to the proliferating cell nuclear antigen (PCNA) replicates the lagging strand and cooperates with flap endonuclease 1 (FEN1) to process the Okazaki fragments for their ligation. (nature.com)
- Missed Cleavage opportunities by FEN1 lead to Okazaki fragments maturations via the long-flap pathway. (edu.sa)
- Loading of the proliferating cell nuclear antigen, PCNA, dissociates DNA polymerase α and recruits DNA polymerase δ and the flap endonuclease FEN1 for elongation and in preparation for its requirement during maturation, respectively. (wustl.edu)
- Nick translation by the strand displacement action of DNA polymerase δ, coupled with the nuclease action of FEN1, results in processive RNA degradation until a proper DNA nick is reached for closure by DNA ligase I. In the event of excessive strand displacement synthesis, other factors, such as the Dna2 nuclease/helicase, are required to trim excess flaps. (wustl.edu)
- The 9-1-1 complex has also been found to interact with base excision repair factors human DNA polymerase beta, flap endonuclease FEN1, and the S. pombe MutY homolog (SpMYH), indicating that 9-1-1 also plays a direct role in DNA repair. (reactome.org)
Polymerase3
- Will the 5'→3' flap endonuclease activity of Taq DNA Polymerase degrade primers? (genscript.com)
- During each PCR cycle, the 5' flap endonuclease domain of Taq DNA polymerase hydrolyzes the probe as the primer is extended and the target sequence is amplified. (neb.com)
- The holoenzyme possesses three activities: 5'→3' DNA-dependent DNA polymerase, 3'→5' proofreading and 3'→5' exonuclease (also known as flap endonuclease). (qiagen.com)
RAD22
- The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. (wikipedia.org)
- Exo1 belongs to the RAD2 family of nucleases and possesses 5-3 nuclease activity and 5-flap endonuclease activity (11,12). (cancercurehere.com)
Single stranded4
- The protein encoded by this gene removes 5' overhanging "flaps" (or short sections of single stranded DNA that "hang off" because their nucleotide bases are prevented from binding to their complementary base pair-despite any base pairing downstream) in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. (wikipedia.org)
- The DNA flap structure occurs in double-stranded DNA containing a single-stranded break where the 5' portion of the downstream strand is too long and overlaps the 3' end of the upstream strand. (musc.edu)
- It is undesirable for DNA sequencing and other applications due to the ability of the 5'→3' exonuclease to degrade flap structures, single-stranded and double-stranded DNA from free 53'→5'-ends. (qiagen.com)
- It is likely that this pathway utilized a flap endonuclease to process the single-stranded DNA that occurs during repair synthesis. (jefferson.edu)
Cryptococcus1
- Flap endonuclease 1 OS=Cryptococcus neoformans var. (uu.nl)
Specificity1
- Sequential and multistep substrate interrogation provides the scaffold for specificity in human flap endonuclease 1.Cell Reports. (edu.sa)
Protein2
- Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. (wikipedia.org)
- DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. (wikipedia.org)
Replication1
- The KLH-cojugated peptide "SIEEIVR", derived from the Flap endonuclease 1 involved in DNA replication and repair. (prosci-inc.com)
Activity1
- Some probe designs do not require the 5' flap endonuclease activity of Taq such as molecular beacons, Scorpions® and dual-hybridization probes. (neb.com)
Structure-specific endonuclease2
- Therefore, we conclude that ChSI is a 5' flap structure-specific endonuclease with nucleolytic activity toward single-stranded substrates. (cnrs.fr)
- Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. (expasy.org)
Apyrimidinic5
- Apurinic / apyrimidinic endonuclease (Ape1) is responsible for the incision of DNA basic sites during base excision repair. (flashcardmachine.com)
- Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. (nih.gov)
- Coordination of steps in single-nucleotide base excision repair mediated by apurinic/apyrimidinic endonuclease 1 and DNA polymerase beta. (neb.com)
- Similarly, we developed assays for several gene families involved in DNA repair: RECQ-family helicases, apurinic/apyrimidinic endonuclease, flap-endonuclease, and translesion polymerases. (nih.gov)
- and a disruptor of the NPM1-APE1 interaction responsible for the nucleolar translocation of the major apurinic/apyrimidinic endonuclease APE1 to effect DNA repair. (nih.gov)
Okazaki3
- The protein encoded by this gene removes 5' overhanging "flaps" (or short sections of single stranded DNA that "hang off" because their nucleotide bases are prevented from binding to their complementary base pair-despite any base pairing downstream) in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. (wikipedia.org)
- 3. Dynamics of enzymatic interactions during short flap human Okazaki fragment processing by two forms of human DNA polymerase δ. (nih.gov)
- During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. (nih.gov)
Interacts2
- Regulatory subunit that interacts with and increases the activity of different structure-specific endonucleases. (expasy.org)
- Interacts with the structure-specific RAD1-RAD10 endonuclease and promotes RAD1-RAD10-dependent 3'-non-homologous tail removal (NHTR) during repair of double-strand breaks by single-strand annealing. (expasy.org)
Polymerase3
- In TaqMan assays the 5' flap endonuclease domain of Taq DNA polymerase cleaves a target specific probe, separating a fluorophore on the 5' end from a quencher on the 3' end resulting in an amplification dependent increase in fluorescence. (neb.com)
- DNA polymerase beta and flap endonuclease 1 enzymatic specificities sustain DNA synthesis for long patch base excision repair. (neb.com)
- AP endonuclease and poly(ADP-ribose) polymerase-1 interact with the same base excision repair intermediate. (neb.com)
Exonuclease activities2
- Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. (nih.gov)
- Uson, M. Loressa , Carl, A. , Goldgur, Y. , and Shuman, S. (2018) Crystal structure and mutational analysis of Mycobacterium smegmatis FenA highlight active site amino acids and three metal ions essential for flap endonuclease and 5' exonuclease activities . (cornell.edu)
Helicase1
- 14. The interaction site of Flap Endonuclease-1 with WRN helicase suggests a coordination of WRN and PCNA. (nih.gov)
Genotypes2
Cleave2
- Flap endonucleases cleave the downstream strand of the overlap flap structure precisely after the first base-paired nucleotide, creating a ligatable nick. (bvsalud.org)
- It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. (nih.gov)
Characterization1
- BK Fortini *, S Pokharel*, P Polaczek*, L Balakrishnan, RA Bambara, and JL Campbell, "Characterization of the Endonuclease and ATP-dependent Flap Endo/exonuclease of Dna2. (kgi.edu)
Substrates2
- The repair of some types of DNA damage has been shown to proceed through branched DNA intermediates which are substrates for the structure-specific DNA endonucleases. (cnrs.fr)
- Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. (expasy.org)
Synthesis1
- Excision of the lesion is achieved by strand displacement synthesis in the presence of flap endonuclease during which C2-AP and the 3'-adjacent nucleotide are replaced. (tamu.edu)
Enzyme2
- The catalytic efficiency (k(cat)/K(M)) of the enzyme is significantly higher for the 5' flap substrate than for single-stranded DNA. (cnrs.fr)
- The findings of the study exposed the flap motions and dynamics of the plasmepsin enzyme, which could potentially help in the design of novel antimalarial compounds. (ukzn.ac.za)
Downstream1
- The DNA flap structure occurs in double-stranded DNA containing a single-stranded break where the 5' portion of the downstream strand is too long and overlaps the 3' end of the upstream strand. (bvsalud.org)
Gene1
- Ejaz, A. , Goldgur, Y. , and Shuman, S. (2019) Activity and structure of MPE, a manganese-dependent single-strand DNA endonuclease encoded in a nucleic acid repair gene cluster . (cornell.edu)
Incision1
- flap incision. (nih.gov)
Single-strand1
- It is a single-strand-specific DNA and RNA endonuclease. (cnrs.fr)
Interactions1
- This is mediated by its flap endonuclease activity independent of its gap endonuclease activity and C-terminal interactions. (wustl.edu)
Catalytic1
- catalytic subunit of the RAD1-RAD10 endonuclease. (expasy.org)
Structural1
- Thus we tested the substrate specificity of ChSI endonuclease toward various branched DNAs containing 5' flap, 5' pseudoflap, 3' pseudoflap, or single-stranded bulged structural motifs. (cnrs.fr)
Function1
- This appears to be required for the function with the RAD1-RAD10 endonuclease. (expasy.org)