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  • double
  • 80. The method of claim 77, wherein the FLAP probe further comprises a 3-prime unpaired segment that overlaps with the 5-prime region of the target probe in the FLAP substrate, thereby forming a double FLAP substrate. (patentsencyclopedia.com)
  • polymerase
  • During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. (uniprot.org)
  • During DNA replication, cleaves the 5'-overhanging flap CC structure that is generated by displacement synthesis when DNA CC polymerase encounters the 5'-end of a downstream Okazaki fragment. (genome.jp)
  • strands
  • The endonuclease activity of FENs was initially identified as acting on a DNA duplex which has a single-stranded 5' overhang on one of the strands (termed a "5' flap", hence the name flap endonuclease). (wikipedia.org)
  • Once aligned, any overhanging bases (flaps) and mismatched bases on the strands are removed and any missing nucleotides are inserted. (wikipedia.org)
  • As this method's only way of identifying if the two strands are related is based on microhomology down/upstream from the site of breakage, it does not identify any missing base pairs which may have been lost during the break, and even removes nucleotides (flaps) in order to ligate the strand. (wikipedia.org)
  • MMEJ ligates the DNA strands without checking for consistency and causes deletions, since it removes base pairs (flaps) in order to align the two pieces. (wikipedia.org)
  • repair
  • Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. (uniprot.org)
  • Mol CD, Hosfield DJ and Tainer JA (2000) Abasic site recognition by two apurinic/apyrimidinic endonuclease families in DNA base excision repair: the 3′ ends justify the means. (els.net)
  • These endonuclease complexes are required for repair of specific types of DNA lesions and critical for cellular responses to replication fork failure. (genecards.org)
  • Nonspecific endonucleases involved in DNA repair can scan DNA for target sequences or damage. (wikipedia.org)
  • single
  • Flap endonucleases have been used in biotechnology, for example the Taqman PCR assay and the Invader single nucleotide polymorphism (SNP) assay. (wikipedia.org)
  • Therefore, bifunctional glycosylases can convert a base lesion into a single-strand break without the need for an AP endonuclease. (wikipedia.org)