• genes
  • Some authors use the term "EST" to describe genes for which little or no further information exists besides the tag. (wikipedia.org)
  • In an effort to determine genes that are expressed in mycelial cultures of Neurospora crassa over the course of the circadian day, we have sequenced 13,000 cDNA clones from two time-of-day-specific libraries (morning and evening library) generating ∼20,000 sequences. (genetics.org)
  • Using these sequences, the representation of genes among the morning and evening sequences, respectively, in most cases does not reflect their expression patterns over the course of the day. (genetics.org)
  • On the basis of these data we predict that a significant proportion of the expressed Neurospora genes may be regulated by the circadian clock. (genetics.org)
  • Highly expressed genes often exhibit a high degree of codon bias whereas lowly expressed genes often contain many alternative synonymous codons [ 5 ]. (pubmedcentralcanada.ca)
  • Nacken WK, Huijser P, Beltran JP, Saedler H, Sommer H (1991) Molecular characterization of two stamen-specific genes, tapl and fil1, that are expressed in the wild type, but not in the deficiens mutant of Antirrhinum majus . (springer.com)
  • They reveal the type and number of genes being expressed in an organism. (wits.ac.za)
  • EST clustering enables efficient discovery of expressed genes based on which several fields rely such as: disease diagnostics, drug discovery, genetic engineering, alternative splicing and many others. (wits.ac.za)
  • Approximately 1400 genes were sequenced, 70% from a cDNA library generated from dissected basal bulbs (containing the pharyngeal gland cells) and 30% from cDNA libraries generated from whole mixed stage nematodes. (brillonline.com)
  • Genes encoding transthyretin-like proteins were abundant in the bulb library and in situ hybridisation confirmed that one of these is expressed in the basal bulb. (brillonline.com)
  • Genes encoding a variety of proteases, which were shown using in situ hybridisation to be expressed in the gut, were also identified. (brillonline.com)
  • In 2008, two human transcriptomes, composed of millions of transcript-derived sequences covering 16,000 genes, were published and, by 2015, transcriptomes had been published for hundreds of individuals. (wikipedia.org)
  • and c) the C-terminal DNA-binding domain, ETS, which binds to the consensus DNA sequence, 5-GGAA/T-3 within a 9-to-10 bp sequence, in the target genes it regulates. (wikipedia.org)
  • These proteins bind specific DNA sequences, including the kappa-B motif (GGGACTTTCC), in the promoters and enhancer regions of several genes and viruses, including human immunodeficiency virus (HIV). (wikipedia.org)
  • proteins
  • Here we describe a collection of 691 sequences from the Western corn rootworm midgut, 27% of which predict proteins with no matches in current databases. (unl.edu)
  • Of the remaining sequences, most predict proteins with either catalytic (62%) or binding (19%) functions, as expected for proteins expressed in the insect midgut. (unl.edu)
  • Mass spectrometric analysis of androgen-regulated proteins in these cells identified the metastasis-suppressor gene NDKA/nm23, a finding that may explain a marked reduction in metastatic potential when these cells express a functional androgen receptor pathway. (garvan.org.au)
  • The protein encoded by this gene is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. (wikipedia.org)
  • Members of the ZAS family, such as ZAS2 (HIVEP2), are large proteins that contain a ZAS domain, a modular protein structure consisting of a pair of C2H2 zinc fingers with an acidic-rich region and a serine/threonine-rich sequence. (wikipedia.org)
  • Approximately
  • The resulting sequence is a relatively low-quality fragment whose length is limited by current technology to approximately 500 to 800 nucleotides. (wikipedia.org)
  • anther
  • GRPs specifically expressed in xylem, phloem, epidermis, anther tapetum and roots have been described. (ufrgs.br)
  • cells
  • citation needed] CD34 is expressed in roughly 20% of murine hematopoietic stem cells, and can be stimulated and reversed. (wikipedia.org)
  • ArgBP2 is highly abundant in cardiac muscle cells at sarcomeric Z-disc structures, and is expressed in other cells at actin stress fibers and the nucleus. (wikipedia.org)
  • In cells that already express high level of Oct3/4, exogenously transfected Oct3/4 will lead to the repression of Rex1. (wikipedia.org)
  • tissue
  • For instance, cancer origin of a library is often mixed with the tissue name (e.g., the tissue name "glioblastoma" indicates that the EST library was sequenced from brain tissue and the disease condition is cancer). (wikipedia.org)
  • Haptoglobin had been shown to be expressed in adipose tissue of cattle as well. (wikipedia.org)
  • whereas
  • It is expressed on lymph node endothelia, whereas the L-selectin to which it binds is on the T cell. (wikipedia.org)
  • This gene, an active X chromosome homolog of the Y chromosome RBMY gene, is widely expressed whereas the RBMY gene evolved a male-specific function in spermatogenesis. (wikipedia.org)
  • databases
  • First opened to outside users in December 1989, the NCBI Experimental BLAST Network Service, running the latest BLAST software on SMP hardware against the latest releases of the major sequence databases, quickly established the NCBI as a convenient, one-stop shop for sequence similarity searching. (wikipedia.org)
  • Using MEGABLAST, Hi-Fi reads are then matched to sequences in annotated databases and any positive matches are then subtracted from the dataset. (wikipedia.org)
  • reads
  • The initial quality screen uses several general parameters to exclude ambiguous sequences, leaving behind a dataset of high-fidelity (Hi-Fi) reads. (wikipedia.org)
  • total
  • The DFA could then be used to scan subject sequences in a single pass with no backtracking in O(m) time, where m is the total length of the subject(s). (wikipedia.org)
  • BLAST
  • At Washington University in St. Louis, Gish revolutionized similarity searching by developing the first BLAST suite of programs to combine rapid gapped sequence alignment with statistical evaluation methods appropriate for gapped alignment scores. (wikipedia.org)
  • generally, the remaining sequence is BLAST again with less stringent parameters to allow for slight mismatches (1 in 20 nucleotide). (wikipedia.org)
  • database
  • For ∼50% of the sequences (710 of 1431), significant matches to sequences in the National Center for Biotechnology Information database (of known or unknown function) were detected. (genetics.org)