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  • 1991
  • Nacken WK, Huijser P, Beltran JP, Saedler H, Sommer H (1991) Molecular characterization of two stamen-specific genes, tapl and fil1, that are expressed in the wild type, but not in the deficiens mutant of Antirrhinum majus . (springer.com)
  • genes that are expressed
  • Knowledge of the genes that are expressed in the insect gut are crucial for understanding basic physiology of food digestion, their interactions with Bacillus thuringiensis (Bt) toxins, and for discovering new targets for novel toxins for use in pest management. (biomedcentral.com)
  • axolotl
  • Expressed sequence tag (EST) markers were developed for Ambystoma tigrinum tigrinum (Eastern tiger salamander) and for A. mexicanum (Mexican axolotl) to generate the first comprehensive linkage map for these model amphibians. (genetics.org)
  • anther
  • GRPs specifically expressed in xylem, phloem, epidermis, anther tapetum and roots have been described. (ufrgs.br)
  • transcriptome
  • Consequently, the manner in which a transcriptome as a whole is expressed and regulated remained unknown until higher-throughput techniques were developed. (wikipedia.org)
  • Cells
  • ArgBP2 is highly abundant in cardiac muscle cells at sarcomeric Z-disc structures, and is expressed in other cells at actin stress fibers and the nucleus. (wikipedia.org)
  • In cells that already express high level of Oct3/4, exogenously transfected Oct3/4 will lead to the repression of Rex1. (wikipedia.org)
  • tissue
  • For instance, cancer origin of a library is often mixed with the tissue name (e.g., the tissue name "glioblastoma" indicates that the EST library was sequenced from brain tissue and the disease condition is cancer). (wikipedia.org)
  • Haptoglobin had been shown to be expressed in adipose tissue of cattle as well. (wikipedia.org)
  • databases
  • First opened to outside users in December 1989, the NCBI Experimental BLAST Network Service, running the latest BLAST software on SMP hardware against the latest releases of the major sequence databases, quickly established the NCBI as a convenient, one-stop shop for sequence similarity searching. (wikipedia.org)
  • Using MEGABLAST, Hi-Fi reads are then matched to sequences in annotated databases and any positive matches are then subtracted from the dataset. (wikipedia.org)
  • reads
  • The initial quality screen uses several general parameters to exclude ambiguous sequences, leaving behind a dataset of high-fidelity (Hi-Fi) reads. (wikipedia.org)
  • database
  • For ∼50% of the sequences (710 of 1431), significant matches to sequences in the National Center for Biotechnology Information database (of known or unknown function) were detected. (genetics.org)
  • several
  • EST clustering enables efficient discovery of expressed genes based on which several fields rely such as: disease diagnostics, drug discovery, genetic engineering, alternative splicing and many others. (wits.ac.za)
  • relatively
  • The resulting sequence is a relatively low-quality fragment whose length is limited by current technology to approximately 500 to 800 nucleotides. (wikipedia.org)
  • BLAST
  • At Washington University in St. Louis, Gish revolutionized similarity searching by developing the first BLAST suite of programs to combine rapid gapped sequence alignment with statistical evaluation methods appropriate for gapped alignment scores. (wikipedia.org)
  • generally, the remaining sequence is BLAST again with less stringent parameters to allow for slight mismatches (1 in 20 nucleotide). (wikipedia.org)
  • among
  • Using these sequences, the representation of genes among the morning and evening sequences, respectively, in most cases does not reflect their expression patterns over the course of the day. (genetics.org)
  • capture
  • To allow capture and purification of generated sequencing products onto paramagnetic beads, samples were analysed using the BigDye Terminator Cycle Sequencing kit and biotinylated sequencing primer before the samples were loaded on ABI 377 DNA sequencer. (ukessays.com)
  • There are two key contemporary techniques in the field: microarrays, which quantify a set of predetermined sequences, and RNA-Seq, which uses high-throughput sequencing to capture all sequences. (wikipedia.org)
  • Human
  • ab94065 is a 293T cell transfected lysate in which Human IKK beta has been transiently over-expressed using a pCMV-IKK beta plasmid. (abcam.com)
  • citation needed] Human HSCs express the CD34 marker. (wikipedia.org)
  • dubious - discuss] However, since version numbers are human-generated, not computer-generated, there is nothing that prevents arbitrary changes that violate such guidelines: for example, the first sequence could be incremented between versions that differ by not even a single line of code, to give the (false) impression that very significant changes were made. (wikipedia.org)
  • Cell
  • Western blot analysis of DUSP22 over-expressed 293 cell line, cotransfected with DUSP22 Validated Chimera RNAi ( Cat # H00056940-R01V ) (Lane 2) or non-transfected control (Lane 1). (abnova.com)
  • TCR first gets expressed on the surface of T cell precursors that also have two co-receptors on their surface named CD4 and CD8. (openwetware.org)
  • single
  • This may be done to emphasize the value of the upgrade to the software user, or, as in Adobe's case, to represent a release halfway between major versions (although levels of sequence based versioning are not limited to a single digit, as in Drupal version 7.12). (wikipedia.org)
  • code
  • For instance, in a scheme that uses a four-sequence identifier, the first sequence may be incremented only when the code is completely rewritten, while a change to the user interface or the documentation may only warrant a change to the fourth sequence. (wikipedia.org)