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  • tissues
  • In the case of solid tissues or cells, these are often first broken down mechanically using a blender (for larger sample volumes), using a homogenizer (smaller volumes), by sonicator or by using cycling of high pressure, and a combination of biochemical and mechanical techniques - including various types of filtration and centrifugation - may be used to separate different cell compartments and organelles prior to electrophoresis. (wikipedia.org)
  • concentration
  • Various experimental parameters, such as the polyacrylamide concentration and voltage applied to the gel are discussed. (jove.com)
  • Diffusion and displacement coefficients may be modified by varying the gel concentration, the intensity of the incident UV radiation and the temperature at which the gel is run. (google.com)
  • shift
  • Gel shift assays are often performed in vitro concurrently with DNase footprinting, primer extension, and promoter-probe experiments when studying transcription initiation, DNA replication, DNA repair or RNA processing and maturation, as well as pre-mRNA splicing. (wikipedia.org)
  • Although
  • Although the cross sectional dimensions of typical microfabricated electrophoresis channels are on the order of those employed in conventional capillary systems, scale-down efforts have proven to be less than straightforward. (google.com)
  • polymers
  • In addition, a gel filtration experiment was performed to see if the underestimation was caused by the presence of albumin polymers in the purified and heat-treated product, which might interfere with sample diffusion in the Vitros slide. (thefreelibrary.com)
  • filtration
  • 254,255 Stereoacuity declines precipitously as a function of the distance of the test objects from the convergence plane (Fig. Determine precise radioligand concentration as in gel filtration protocol above. (imbc-med.ru)
  • water
  • After repeated washing with water, the gel is incubated in a silver nitrate solution. (wikipedia.org)