• Very often, CE refers to capillary zone electrophoresis (CZE), but other electrophoretic techniques including capillary gel electrophoresis (CGE), capillary isoelectric focusing (CIEF), capillary isotachophoresis and micellar electrokinetic chromatography (MEKC) belong also to this class of methods. (wikipedia.org)
  • Capillary zone electrophoresis was employed for the determination of myoglobin in human urine using end-column amperometric detection with a carbon fiber microelectrode at a constant potential of 1.80 V vs. saturated calomel electrode (SCF). (unboundmedicine.com)
  • An acid dissociation constant (p K a ) of bupropion has been determined in an aqueous solution by capillary zone electrophoresis (CZE). (go.jp)
  • Capillary zone electrophoresis-electrospray ioniza. (analyteguru.com)
  • Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry for quantitative parallel reaction monitoring of peptide abundance and single-shot proteomic analysis of a human cell line. (analyteguru.com)
  • We coupled capillary zone electrophoresis (CZE) with an ultrasensitive electrokinetically pumped nanospray ionization source for tandem mass spectrometry (MS/MS) analysis of complex proteomes. (analyteguru.com)
  • Two original products, Eprex (epoetin alfa) and Dynepo (epoetin delta), and two biosimilar products, Binocrit (epoetin alfa) and Retacrit (epoetin zeta), were compared using (1) high performance size exclusion chromatography, (2) ELISA, (3) SDS-PAGE, (4) capillary zone electrophoresis and (5) in-vivo potency. (springer.com)
  • Because a limited amount of EPO products was available, we were restricted to the common assays used for quality control: high performance size exclusion chromatography (HP-SEC) to assess monomer and aggregate content, ELISA to determine EPO protein content, SDS-PAGE under non-reducing conditions to detect possible fragmentation and covalent protein aggregates, capillary zone electrophoresis (CZE) for isoform profiling and a normocythemic mouse assay to test for potency. (springer.com)
  • Recent advances have stimulated interest concerning the development of microcolumn liquid-phase separation methods, including capillary liquid chromatography in various forms, and capillary zone electrophoresis. (psu.edu)
  • Comparison of high performance liquid chromatography with capillary zone electrophoresis. (cdc.gov)
  • In this work, the analysis of the urine oligosaccharide pattern was standardized for the first time by using capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection (Beckman P/ACE MDQ) with a 488-nm argon ion laser module. (nih.gov)
  • We have developed a method using capillary electrophoresis and laser-induced fluorescence to detect YOYO-1 complexes of DNA produced by PCR. (elsevierpure.com)
  • In this study, researchers from Okayama University developed a method to determine the EV membrane protein, CD63, by coupling capillary electrophoresis immunoassay with laser-induced fluorescence (CEIA-LIF). (exosome-rna.com)
  • The analytes separate as they migrate due to their electrophoretic mobility, and are detected near the outlet end of the capillary. (wikipedia.org)
  • To circumvent these drawbacks, we have developed a hybrid platform consisting of simple microfabricated reservoirs connected to standard capillary tubing for electrophoretic separation and in-channel detection. (labsmith.com)
  • Certain aspects of the instrumentation (such as detection) are necessarily more complex than for a single-capillary system, but the fundamental principles of design and operation are similar to those shown in Figure 1. (wikipedia.org)
  • Separation by capillary electrophoresis can be detected by several detection devices. (wikipedia.org)
  • In these systems, a section of the capillary itself is used as the detection cell. (wikipedia.org)
  • The portion of the capillary used for UV detection, however, must be optically transparent. (wikipedia.org)
  • The path length of the detection cell in capillary electrophoresis (~ 50 micrometers) is far less than that of a traditional UV cell (~ 1 cm). (wikipedia.org)
  • The new extraction and detection method employs capillary electrophoresis with electrospray ionization mass spectrometry (CE-ESI MS) and was specifically developed to determine polar organic compounds in the ACI PM emissions. (sae.org)
  • Detection of Polar Compounds Condensed on Particulate Matter Using Capillary Electrophoresis-Mass Spectrometry," SAE Technical Paper 2020-01-0395, 2020, https://doi.org/10.4271/2020-01-0395 . (sae.org)
  • A1 - Lurie,I S, PY - 1996/9/1/pubmed PY - 1997/12/31/medline PY - 1996/9/1/entrez SP - 237 EP - 42 JF - Journal of capillary electrophoresis JO - J Capillary Electrophor VL - 3 IS - 5 N2 - Methodology is presented for the analysis of cations and anions in illicit heroin using CE with indirect UV detection. (unboundmedicine.com)
  • To test this hypothesis, we used a rat model of endurance exercise, a microwave irradiation for accurate detection of glycogen in the brain (the cortex, hippocampus, and hypothalamus), and capillary electrophoresis mass spectrometry-based metabolomics to observe the comprehensive metabolic profile of the blood. (frontiersin.org)
  • Capillary gel electrophoresis generally allows higher resolution, higher detection sensitivity and easier integration with other separation and detection techniques. (bvsalud.org)
  • Aliquots were removed from the reaction mixture at specific time intervals and analyzed on a 57-cm untreated silica capillary for the presence of 7-hydroxycoumarin-glucuronide. (dcu.ie)
  • TMean was found to be up to 20 oC higher than the external temperature under typical conditions using active air-cooling and a 74.0 ˜í¬¿m internal diameter (di) fused-silica capillary. (edu.au)
  • A combination of experimentally-determined and calculated temperatures enabled a complete temperature profile for a fused-silica capillary to be drawn, the thickness of the stationary air layer to be determined and the heat transfer coefficient (h) for the capillary to be determined. (edu.au)
  • A capillary electrophoresis (CE) way for the characterization of recombinant NTPDases 1, 2, and 3, as well as for assaying NTPDase inhibitors continues to be developed executing the enzymatic response inside the capillary. (bioinf.org)
  • Rotavirus double and triple layered viral particles: correlative characterization using electron microscopy, disc centrifuge and capillary electrophoresis. (emc-proceedings.com)
  • Dublin, Aug. 31, 2023 (GLOBE NEWSWIRE) -- The "Capillary Electrophoresis - Global Strategic Business Report" report has been added to ResearchAndMarkets.com's offering. (globenewswire.com)
  • Electrophoresis;2023 Aug 24. (bvsalud.org)
  • Capillary electrophoresis (CE) is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro- and nanofluidic channels. (wikipedia.org)
  • The course presents six different methods of capillary electrophoresis, starting with the theoretical foundations and explaining various pharmaceutical applications. (pte.hu)
  • Read the related article, Microfab-less microfluidic capillary electrophoresis devices , Analytical Methods 2013, Thiago P. Segato,a Samir A. Bhakta,Matthew T. Gordon,Emanuel Carrilho,Peter A. Willis,Hong Jiaod and Carlos D. Garcia. (labsmith.com)
  • Both gel and capillary methods evaluated polymorphic regions of the merozoite surface protein 1 and 2 and glutamine rich protein genes. (biomedcentral.com)
  • Risk differences between AL and DP were not significantly different whether gel or capillary methods were used. (biomedcentral.com)
  • In response to limitations in standard genotyping methods, the World Health Organization (WHO) recently recommended genotyping with capillary electrophoresis, where possible, to increase test sensitivity and discriminatory power [ 6 ]. (biomedcentral.com)
  • Confirmation testing of urinary amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine (MDMA, Ecstasy) and 3,4-methylenedioxyamphetamine (MDA) by capillary electrophoresis (CE) combined with atmospheric pressure electrospray ionization and ion trap mass spectrometry (MS) is described. (erowid.org)
  • Using the optimized circumstances, Michaelis-Menten constants (ideals for NTPDase1, 2 and 3 acquired for reactive blue 2, PPADS, suramin, and "type":"entrez-protein","attrs":"text message":"ARL67156″,"term_id":"1186396857″,"term_text message":"ARL67156″ARL67156, using the in-capillary electrophoresis technique. (bioinf.org)
  • Capillary electrophoresis (CE) nanoelectrospray ionization (nanoESI) provides high-resolution mass spectrometry (HRMS) with trace-level sensitivity, but compressed separation during CE challenges protein identification by tandem HRMS with limited MS/MS duty cycle. (researchwithrutgers.com)
  • Instituto de Investigación en the protein rOmpA. (cdc.gov)
  • The source vial, destination vial and capillary are filled with an electrolyte such as an aqueous buffer solution. (wikipedia.org)
  • Sharing the same technical platform and reagent line, laboratories can mix the different CAPILLARYS 3 platforms to meet their individual needs in production capacity, in addition to the large automated capillary electrophoresis menu. (sebia.com)
  • Enantiomers of amino acids and peptides were derivatized with a fluorescent chiral reagent 4-(3-isothiocyanatopyrrolidin-1-yl)-7-nitro- 2,1,3-benzoxadiazole [R-(-)- or S-(+)-NBD-PyNCS] and the resulting diastereomeric derivatives separated by capillary electrophoresis (CE). (illinois.edu)
  • Sample is introduced into the capillary via capillary action, pressure, siphoning, or electrokinetically, and the capillary is then returned to the source vial. (wikipedia.org)
  • Capillary electrophoresis can be used as a modern analytical separation technique for the separation and determination of metal ions, inorganic anions, chiral compounds, peptides and proteins, carbohydrates, and even whole cells and viruses. (pte.hu)
  • The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE fo. (firebaseapp.com)
  • Capillary electrophoresis (CE) is a brand-new analytical me- thod with the capability of solving many analytical separa- tion problems very fast and economically. (loancompaniesnearme.com)
  • Kinetic capillary electrophoresis (KCE) is an analytical platform that offers significant advantages in studying the thermodynamic and kinetic parameters of molecular interactions. (bvsalud.org)
  • Study of molecular interactions by nonequilibrium capillary electrophoresis of equilibrium mixtures: Originations, developments, and applications. (bvsalud.org)
  • Nonequilibrium capillary electrophoresis of equilibrium mixtures (NECEEM) was the first proposed KCE method , and it has found widespread use in studying molecular interactions involving proteins /aptamers, proteins /small molecules, and peptides /small molecules. (bvsalud.org)
  • In the most common mode of CE, all ions, positive or negative, are pulled through the capillary in the same direction by electroosmotic flow. (wikipedia.org)
  • The system's main components are a sample vial, source and destination vials, a capillary, electrodes, a high voltage power supply, a detector, and a data output and handling device. (wikipedia.org)
  • The connections for this hybrid device are assembled using a reusable, conventionally machined CapTite™ Ultem® cross interconnect and CapTite™ PEEK™ capillary fittings. (labsmith.com)
  • The present invention is an ultra-compact capillary electrophoresis (CE) device that can operate both independently and in connection with any mass spectrometer (MS) using a product-specific adapter that can be mounted on the CE device. (danubia.com)
  • The UCESI device (100) consists of a product-specific mounting frame (101), a thermostatable capillary module (102), an ESI port (103), a movable sample tray (104), and a serving unit (105). (danubia.com)
  • Capillary electrophoresis was first combined with mass spectrometry by Richard D. Smith and coworkers, and provides extremely high sensitivity for the analysis of very small sample sizes. (wikipedia.org)
  • The global market perspective on capillary electrophoresis reveals a comprehensive analysis of annual sales from 2014 to 2030. (globenewswire.com)
  • In the changed post COVID-19 business landscape, the global market for Capillary Electrophoresis estimated at US$964.2 Million in the year 2022, is projected to reach a revised size of US$1.5 Billion by 2030, growing at a CAGR of 5.7% over the analysis period 2022-2030. (globenewswire.com)
  • For anions, proprietary reagents were used, including the Anitron run buffer (PE Applied Biosystems, Foster City, CA, U.S.A.) and Micro-Coat capillary charge-reversal agent (PE Applied Biosystems), which was utilized to flush the capillary prior to each analysis. (unboundmedicine.com)
  • Further confirmation of results can be carried out by capillary electrophoresis (and/or GC/MS or MS/MS). In 1998, the prevalence of positives for morphine, cocaine and ecstasy was 4.8, 11.3 and 2.6%, respectively. (erowid.org)
  • Despite the very small sample sizes (typically only a few nanoliters of liquid are introduced into the capillary), high sensitivity and sharp peaks are achieved in part due to injection strategies that result in a concentration of analytes into a narrow zone near the inlet of the capillary. (wikipedia.org)
  • A free zone capillary electrophoresis method was developed for the quantitation of cocaine in illicit powders. (astm.org)
  • METHOD: Urine from patients with biopsy-proven IgAN was analyzed using capillary electrophoresis-mass spectrometry at baseline (IgAN237-1, n = 103) and at follow-up (IgAN237-2, n = 89). (bvsalud.org)
  • To achieve greater sample throughput, instruments with arrays of capillaries are used to analyze many samples simultaneously. (wikipedia.org)
  • Such capillary array electrophoresis (CAE) instruments with 16 or 96 capillaries are used for medium- to high-throughput capillary DNA sequencing, and the inlet ends of the capillaries are arrayed spatially to accept samples directly from SBS-standard footprint 96-well plates. (wikipedia.org)
  • With 8 capillaries, the CAPILLARYS 3 OCTA reduces hands-on-time and offers a large loading capacity with 120 samples (15 racks). (sebia.com)
  • Samples were genotyped using both gel and capillary electrophoresis from randomized trials of artemether-lumefantrine (AL) vs. dihydroartemisinin-piperaquine (DP) performed in two areas of Uganda: Kanungu, where transmission is moderate, and Apac, where transmission is very high. (biomedcentral.com)
  • This bare section of capillary can break easily, and capillaries with transparent coatings are available to increase the stability of the cell window. (wikipedia.org)
  • For polyimide-coated capillaries, a segment of the coating is typically burned or scraped off to provide a bare window several millimeters long. (wikipedia.org)
  • Whilst temperature control is usually employed in capillary electrophoresis (CE) to aid heat dissipation and provide acceptable precision, internal electrolyte temperatures are almost never measured. (edu.au)
  • Macrogen has extensive experience and automated systems to provide quality Capillary Electrophoresis Sequencing (CES) service. (macrogen-apac.com)
  • To introduce the sample, the capillary inlet is placed into a vial containing the sample. (wikipedia.org)
  • After removing the EVs containing the CD63 immune complex by centrifugation, the supernatant containing the free fluorescent antibody was injected into a capillary to serve as a sample. (exosome-rna.com)
  • This report offers a comprehensive understanding of the capillary electrophoresis market, aiding strategic decision-making for industry stakeholders. (globenewswire.com)