• After incubation with an anti-digoxigenin antibody Xanthone (Genicide) conjugated with alkaline phosphatase (Boehringer Mannheim), membranes had been immersed in the chemiluminescent substrate, Xanthone (Genicide) CSPD (Tropix, Bedford, MA), and subjected to Fuji brand-new RX x-ray film (Fuji Image Film, Kanagawa, Japan). (mingsheng88.org)
  • The probe was labeled with digoxigenin, and hybridized fragments were detected with anti-digoxigenin antibody. (upenn.edu)
  • Kd of the digoxigenin-antibody interaction has been estimated at ~12 nM (compare to Kd~0.1pM for the biotin-streptavidin interaction). (wikipedia.org)
  • Lanatoside C). Digoxigenin is a hapten, a small molecule with high antigenicity, that is used in many molecular biology applications similarly to other popular haptens such as 2,4-Dinitrophenol, biotin, and fluorescein. (wikipedia.org)
  • The HER-2/neu gene was localized using the Oncor (Gaithersburg, MD) digoxigenin-labeled unique sequence probe. (nih.gov)
  • A Digoxigenin-labeled probe derived from EHV 1 was used for hybridization. (uni-muenchen.de)
  • The specificity of the product was confirmed by hybridization to a digoxigenin-labeled probe according to the method described in Boehringer Mannhein's Genius System User's Guide for Filter Hybridization. (cdc.gov)
  • NGRL has been granted the license by "Roche Diagnostics GmbH" with regard to synthesis and sale and offering for sale of Digoxigenin labeling oligonucleotide(s) since 8th Dec. 1995. (ngrl.co.jp)
  • Anti-digoxigenin antibodies with high affinities and specificity are used in a variety of biological immuno-assays (e.g. (wikipedia.org)
  • The hybridized digoxigenin-labeled DNA probes can be detected by their interaction with antibodies coupled to fluorescent dyes or color-producing enzymes. (enzolifesciences.com)
  • Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea, Digitalis orientalis and Digitalis lanata (foxgloves), where it is attached to sugars, to form the glycosides (e.g. (wikipedia.org)
  • Optimization of a digoxigenin-based immunoassay system for gene detection in Arabidopsis thaliana" (pdf). (wikipedia.org)
  • Digoxigenin (DIG) is a small plant-derived molecule not found in animals. (vectorlabs.com)
  • The most commonly used labels for DNA probes include radioisotopes like Phosphorus 32, fluorophores like fluorescein, or small molecule binding partners such as biotin and digoxigenin. (jove.com)
  • Intracellular localization of parvovirus B19 nucleic acid at the ultrastructural level by in situ hybridization with digoxigenin-labelled probes. (ox.ac.uk)
  • Conditions suitable for immunogold detection of digoxigenin-labelled DNA probes hybridized to parvovirus B19-infected erythroid cells embedded in Lowicryl K4M and LR White acrylic resins were established at the electron microscope level. (ox.ac.uk)
  • We describe a method for direct QD labeling of modified oligonucleotide probes through streptavidin-biotin and antibody-mediated interactions (anti-FITC and anti-digoxigenin). (hindawi.com)
  • One of the probes is immobilized in microtiter wells, via the digoxigenin/anti-digoxigenin interaction, and the other probe is biotinylated. (uncg.edu)
  • We target the gene of interest with dsDNA polynucleotide probes labeled multiple digoxigenins to which horse-raddish-peroxidase labeled antibodies bind. (mpi-bremen.de)
  • Detection reagents are free for digoxigenin labeled probes. (pantomics.com)
  • In this article we describe a way for colorimetric recognition of miRNA in the kidney through hybridization with digoxigenin tagged microRNA probes. (immune-source.com)
  • Though we have found the 5' digoxigenin conjugated probes to provide us with sufficient detection sensitivity for our applications in kidney. (immune-source.com)
  • Anti-digoxigenin antibodies with high affinities and specificity are used in a variety of biological immuno-assays (e.g. (wikipedia.org)
  • L'étude menée en Iraq a utilisé la méthode d'hybridation in situ pour déterminer la fréquence du papillomavirus humain et pour son génotypage dans les échantillons de tissus prélevés auprès de 129 patientes ayant reçu un diagnostic de cancer du sein malin, de 24 patientes porteuses d'une tumeur du sein bénigne et de 20 femmes témoins en bonne santé. (who.int)
  • Optimization of a digoxigenin-based immunoassay system for gene detection in Arabidopsis thaliana" (pdf). (wikipedia.org)
  • Incorporation of digoxigenin-11-dUTP during amplification allowed direct detection and quantitation of mRNA levels by chemiluminescence. (jci.org)
  • Typically, digoxigenin is introduced chemically (conjugation) into biomolecules (proteins, nucleic acids) to be detected in further assays. (wikipedia.org)
  • Knockdown of NEIL1 in LN428 glioblastoma cells resulted in enhanced recruitment of XPC, a more rapid removal of digoxigenin-tagged psoralen adducts, and decreased cellular sensitivity to trioxsalen, implying that NEIL1 and BER may interfere the processing of ICLs. (nih.gov)