• hexokinase
  • 2-Deoxyglucose (2DG) was detected by measuring a potent fluorophore, resorufin, generated after incubation with a single assay solution containing hexokinase, adenosine 5'-triphosphate, glucose 6-phosphate dehydrogenase, beta-nicotineamide adenine dinucleotide phosphate, diaphorase, and resazurin. (sigmaaldrich.com)
  • DG is transported into the brain via the same mechanism which transports glucose, where it is then phosphorylated by hexokinase into 14C-deoxyglucose-6-phosphate(DG-6-P). The accumulation of DG-6-P reflects the rate of glucose consumption, and can be quantitatively measured using an autoradiograph. (wikipedia.org)
  • enzymology
  • In enzymology, a 2-deoxyglucose-6-phosphatase (EC 3.1.3.68) is an enzyme that catalyzes the chemical reaction 2-deoxy-D-glucose 6-phosphate + H2O ⇌ {\displaystyle \rightleftharpoons } 2-deoxy-D-glucose + phosphate Thus, the two substrates of this enzyme are 2-deoxy-D-glucose 6-phosphate and H2O, whereas its two products are 2-deoxy-D-glucose and phosphate. (wikipedia.org)