• Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Polymerase DNA Directed Delta 1 (POLd) in Tissue homogenates, cell lysates and other biological fluids. (myelisakit.com)
  • With a combinatorial approach to redesign an enzyme motif the mismatch extension selectivity of DNA polymerases was increased. (uni-konstanz.de)
  • The multisubunit RNA polymerase (RNAP) is the central enzyme responsible for RNA synthesis in bacteria. (nih.gov)
  • In 1970 Temin and Japanese virologist Satoshi Mizutani, and American virologist David Baltimore , working independently, reported the discovery of an enzyme that could synthesize proviral DNA from the RNA genome of RSV. (britannica.com)
  • This enzyme was named RNA-directed DNA polymerase, commonly referred to as reverse transcriptase. (britannica.com)
  • Nucleoside reverse transcriptase inhibitors (NRTIs) such as AZT (zidovudine)-the first drug approved by the U.S. Food and Drug Administration to prolong the lives of AIDS patients-act by terminating the proviral DNA chain before the enzyme can finish transcription. (britannica.com)
  • The solution structure of the amino-terminal domain of human DNA polymerase epsilon subunit B is homologous to C-domains of AAA+ proteins. (nih.gov)
  • Purification, cDNA cloning, and gene mapping of the small subunit of human DNA polymerase epsilon. (nih.gov)
  • Reference: Stimulation of Rauscher leukemia virus DNA polymerase DNA-directed DNA synthesis by cationic trypanocides and polyamines. (neb.com)
  • Activated DNA-directed DNA synthesis catalyzed by Rauscher leukemia virus (RLV) and other type C mammalian retroviral DNA polymerases is uniquely stimulated by biologically active polyamines. (neb.com)
  • As described here, several cationic trypanocides stimulate RLV polymerase-catalyzed DNA-directed DNA synthesis at concentrations significantly inhibiting eukaryotic DNA polymerases. (neb.com)
  • After second- strand cDNA synthesis, T7 RNA polymerase was used to generate aRNA. (genomicglossaries.com)
  • Polymerase (DNA directed) nu is a protein in humans that is encoded by the POLN gene. (wikipedia.org)
  • The canonical function of a bacterial sigma (σ) factor is to determine the gene specificity of the RNA polymerase (RNAP). (nih.gov)
  • DNA polymerase epsilon, which is involved in DNA repair and replication, is composed of a large catalytic subunit and a small accessory subunit. (nih.gov)
  • Mutations/polymorphisms in the 55 kDa subunit of DNA polymerase epsilon in human colorectal cancer. (nih.gov)
  • Download DNA or protein sequence, view genomic context and coordinates. (yeastgenome.org)
  • 87(5): 1663-1667] Specifically, the investigators started by priming whole cerebellar RNA with a synthetic oligonucleotide containing a T7 RNA polymerase promoter sequence. (genomicglossaries.com)
  • GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. (genscript.com)
  • With a PCR-free approach, using enrichment of the viral DNA, we acquired as much as 121 kb of nucleotide sequence, almost the full genome, with coverage values of ≈100 in 5 contigs (BioProject no. (cdc.gov)
  • The dissociable sigma (σ) factor subunit directs the RNAP to different sets of genes to allow their expression in response to various cellular needs. (nih.gov)
  • Homologous to Y-family DNA polymerases, contains BRCT domain. (utoronto.ca)
  • Human IgG antibody Laboratories manufactures the direct elisa protein reagents distributed by Genprice. (taq-polymerase-dna.com)
  • It is a family A DNA polymerase, considered to be the least effective of the polymerase enzymes. (wikipedia.org)
  • For this purpose, dUTP derivatives were tested as electroactive 'labels' by square wave voltammetry on carbon screen printed electrodes and as proper substrates for polymerase enzymes in PCR and RPA methods. (ac.ru)
  • Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. (cathdb.info)
  • Direct electrochemical detection of double-stranded DNA (dsDNA) may provide a reliable and cost-effective approach to quantify DNA amplification products, with a great potential for coupling to isothermal amplification techniques [1]. (ac.ru)
  • The aim of this work was to combine a set of novel 2'-deoxyuridine-5'-triphosphate (dUTP) derivatives modified with Tyr or Trp aromatic groups with polymerase chain reaction (PCR) and isothermal amplification technique such as recombinase polymerase amplification (RPA). (ac.ru)
  • The cDNA can then be amplified by polymerase chain reaction and used for subsequent experiments. (britannica.com)
  • Specific molecular analysis is made by single-strand confirmation polymorphism (SSCP), direct DNA analysis, denaturing gradient gel electrophoresis, or reverse transcriptase-polymerase chain reaction to search for the BTK mutation. (medscape.com)
  • A multiplex polymerase chain reaction (PCR) assay was also developed as an alternative for identification of isolates, and was shown to be a rapid, sensitive and specific diagnostic assay. (who.int)
  • The tiplex polymerase chain reaction (PCR) spores can survive for many years in soil. (who.int)
  • RNA polymerase (RNAP) performs various tasks during transcription by changing its conformational states, which are gradually becoming clarified. (nih.gov)
  • Because RNA is fragile and difficult to study, a strand of complementary DNA (cDNA) is synthesized from RNA, using reverse transcriptase during the RT-PCR procedure. (britannica.com)
  • Description: A sandwich quantitative ELISA assay kit for detection of Human Polymerase DNA Directed Gamma 1 (POLg1) in samples from tissue homogenates, cell lysates or other biological fluids. (myelisakit.com)
  • The ability of reverse transcriptase to synthesize DNA from RNA has been used in the laboratory. (britannica.com)
  • The polymerase domain (EC 2.7.7.49) occupies the N-terminal two- thirds of the reverse transcriptase whereas the ribonuclease H domain comprises the C-terminal remaining one-third. (cathdb.info)
  • RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. (genomicglossaries.com)
  • However, DNA polymerase nu plays an active role in homology repair during cellular responses to crosslinks, fulfilling its role in a complex with helicase. (wikipedia.org)
  • Kinetic analysis of the stimulation of RLV DNA polymerase by three structurally dissimilar cationic trypanocides (Antrycide, Burroughs-Wellcome Compound 64A, and Bayer Compound 1694) suggests that such stimulation is, in part, due to a drug:DNA structural interaction resembling the polyamine:DNA structural complex recognized by the RLV DNA polymerase. (neb.com)
  • Canis lupus familiaris DNA polymerase eta (POLH), transcript variant X4, mRNA. (genscript.com)
  • Catalyzes RNA-template-directed extension of the 3'-end of a DNA strand by one deoxynucleotide at a time. (cathdb.info)
  • aRNA is RNA that is transcribed from the coding, rather than the template, strand of DNA. (genomicglossaries.com)
  • The results impart new and deeper insight into the mechanisms of DNA polymerases and provide new tools for applications such as efficient genotyping. (uni-konstanz.de)
  • Any process that activates or increases the frequency, rate or extent of DNA-directed DNA polymerase activity. (mcw.edu)
  • This is a secondary reaction to the RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end performed by EC 3.1.26.13. (cathdb.info)
  • Use OR to search for either of two terms (e.g. fly OR drosophila ) or quotation marks to search for phrases (e.g. "dna binding" ). (utoronto.ca)
  • Evidence of Cryptosporidium organisms or DNA in stool, intestinal fluid, tissue samples, biopsy specimens, or other biological sample by certain laboratory methods with a high positive predictive value (PPV), e.g. (cdc.gov)