• Although the ITS primers are universal for fungi, the D1D2 region of the large ribosomal subunit has better discrimination for yeasts, with primers NL-1 and NL-4. (cdc.gov)
  • A rapid diagnostic 'mulitplex' one-step polymerase chain reaction protocol (RD-PCR) was developed using cytochrome oxidase subunit 1 (COX1) mitochondrial DNA (mtDNA) to discriminate between S. haematobium and S. bovis. (imperial.ac.uk)
  • NCBI-GenBank lists only five gene fragments, a total of 3534 bp as follows: 1122 bp of DNA dependent RNA Polymerase II second largest subunit, 1069 bp of rRNA subunits/ITS regions, 451 bp of calmodulin, 354 bp of histone-H3, 321 bp of elongation factor 1-alpha and 217 bp of the actin gene. (biomedcentral.com)
  • Polymerase chain reaction (PCR) and DNA sequencing of the internal transcribed spacer (ITS) and 28S large subunit (LSU) regions of the ribosomal DNA, two commonly used DNA barcoding regions for fungi, can also be used to identify M. titans (DeLong and Brewer 2013). (ufl.edu)
  • Sequencing of the large subunit region of ribosomal (r)DNA places the fungus close to the Pezizellaceae, Porodiplodiaceae, and Sclerotiniaceae, with analyses of internal transcribed spacer regions of rDNA indicating that it has affinities to previously unnamed soil and root fungi from alpine, cool temperate and Low Arctic regions. (bas.ac.uk)
  • Phylogenetic relationships in Leymus (Poaceae: Triticeae) revealed by nuclear ribosomal internal transcribed spacer and chloroplast trnL-F sequences. (degruyter.com)
  • We used three chloroplast (cpDNA) fragments ( psb A- trn H, trn L- rpl 32 and trn Q-5′ rps 16), the nuclear ribosomal internal transcribed spacer (nrITS), and co-dominant SSR and dominant ISSR markers to study natural hybridization between L. duciformis and L. paradoxa growing sympatrically in two locations. (peerj.com)
  • it does not describe the procedure for purification of fungal DNA. (cdc.gov)
  • DNA-based identification of fungi in the environment can aid in the diagnosis of plant and animal diseases, enhance studies of fungal diversity and evolution, and lead to a better understanding of factors influencing nutrient cycling and productivity in forests and agricultural ecosystems. (berkeley.edu)
  • This document describes some of the target genes and primers that can be used for DNA sequence-based identification of fungi and the PCR conditions with which to use those primers. (cdc.gov)
  • Phylogenetic dendrograms for 5442 bp and 4401 bp of RNA Polymerase II largest and second largest subunits, and for 5474 bp of the ribosomal RNA cistron of C. personatum are presented in relation to closely related fungi. (biomedcentral.com)
  • because mushrooms are only produced seasonally and sometimes very rarely, e.g. only once every several years, the resulting database will facilitate identification of fungi present in plants, in the soil, and in the air at all times, simply by comparing DNA information with the one generated by this and other studies. (berkeley.edu)
  • The DNA sequence data generated by this project are now available to the public and research communities and can enhance the ability to identify fungi in the environment. (berkeley.edu)
  • However, sequences of the more variable first internal transcribed spacer portion of the ribosomal DNA repeat support its differentiation from the other species of the genera. (usda.gov)
  • Kretzer A., Li Y., Szaro T.M., Bruns T.D., Internal transcribed spacer sequences from 38 recognized species of Suillus sensu lato: Phylogenetic and taxonomic implications, Mycologia 88 (1996) 776-785. (afs-journal.org)
  • For more information, see Calviño and Downie (2007), Circumscription and phylogeny of Apiaceae subfamily Saniculoideae based on chloroplast DNA sequences, Molecular Phylogenetics and Evolution 44: 175-191. (illinois.edu)
  • 2001) recently discussed phylogenetic relationships inferred from parsimony analysis of nucleotide sequences of the internal transcribed spacer regions (ITS) of the 18S-26S nuclear ribosomal DNA of 34 species of Salsola and related genera ( Halothamnus Jaubert & Spach, Climacoptera Botschantzev, Girgensohnia Bunge, Halocharis Moquin-Tandon, and Haloxylon Bunge) and four species from representative outgroups (tribes Camphorosmeae and Atripliceae). (efloras.org)
  • During our initial phylogenetic study of the monocot genus Erythronium (Liliaceae), we observed peculiar eudicot-type internal transcribed spacer (ITS) sequences in a dataset derived from genomic DNA of Erythronium dens-canis. (cas.cz)
  • Methods: S. mutans samples were isolated from the saliva of 30 children with varying histories of dental caries, and they were characterized according to morphological and biochemical markers and the sequences of their 16S-23S intergenic spacer region. (bvsalud.org)
  • Combined with an internal transcribed spacer 2 (ITS2) sequence characters and chemical PCA results, six cultivars were classified into their own groups, for the two species in particular. (frontiersin.org)
  • Trichophyton species DNA is amplified very poorly by the ITS primer set used for most other molds. (cdc.gov)
  • For identification of Trichosporon species, the intergenic region of the ribosomal cistron is used (Sugita, 2002). (cdc.gov)
  • Genomicand genetic relationships among species of Leymus (Poaceae: Triticeae) inferred from 18S-26S ribosomal genes. (degruyter.com)
  • In recent years, DNA barcoding methods, which are used to identify species diversity in the Tree of Life, have been also applied to botanicals and plant-derived dietary supplements. (thieme-connect.com)
  • In this study, we selected an internal transcribed spacer 2 (ITS2) for distinguishing these indirubin-containing species from five of their usual adulterants, after assessing identification efficiency of matK, rbcL, psbA-trnH , and ITS2 among these species. (hindawi.com)
  • The results of genetic distances and neighbor-joining (NJ) phylogenetic tree indicated that ITS2 region is a powerful DNA barcode to accurately identify these indirubin-containing species and discriminate them from their adulterants. (hindawi.com)
  • Henrion B., Chevalier G., Martin F., Typing truffle species by PCR amplification of the ribosomal DNA spacers, Mycol. (afs-journal.org)
  • The only internal transcribed spacer region variation detected between the two species was the additional six initial nucleotides. (scielo.br)
  • Role of unequal crossing-over in the evolution of ribosomal genes. (cnr.it)
  • Identification of transcription and processing signals of ribosomal genes of D. melanogaster. (cnr.it)
  • A non-coding intergenic region between the mitochondrial cytochrome oxidase ( cox )2 and cox 3 genes was also investigated, but because there were extremely low levels of intraspecific variation, the spacer was not useful for phylogeographical analysis. (degruyter.com)
  • Downregulation of ribosomal RNA (rRNA) genes in human head and neck squamous cell carcinoma (HNSCC) cells correlates with rDNA promoter hypermethylation. (wroc.pl)
  • Ribosomal RNA (rRNA) intergenic spacer analysis (RISA) is a method of microbial community analysis that provides a means of comparing differing environments or treatment impacts without the bias imposed by culture- dependent approaches. (wikipedia.org)
  • RISA involves PCR amplification of a region of the rRNA gene operon between the small (16S) and large (23S) subunits called the intergenic spacer region ISR. (wikipedia.org)
  • To investigate the Ns genome donor of Leymus and to elucidate the origin of Xm genome of Leymus , Random Amplified Polymorphic DNA (RAPD) markers were used to analyze 13 Leymus accessions, together with 14 taxa from related Triticeae genera. (degruyter.com)
  • A diverse representation of the Venice collection was sampled and sent to U.C Berkeley, where a portion of the genome (the ribosomal DNA internal transcribed spacer, or ITS, region) was sequenced and analyzed. (berkeley.edu)
  • This raised the possibility of horizontal transfer of a eudicot alien ribosomal DNA (rDNA) into the Erythronium genome. (cas.cz)
  • Variations in ribosomal DNA copy numbers in a genome of Trichophyton interdigitale. (wroc.pl)
  • DNA barcoding technology offers the best technique for this to date, involving specific amplification of a short, standardized DNA fragment with universal primers across multiple samples [ 19 , 20 ]. (hindawi.com)
  • L'analyse du polymorphisme de la longueur des fragments de restriction par amplification en chaîne par polymérase de l'espaceur interne transcrit 1 au sein du gène d'ARN ribosomique (ITS PCR - RFLP) a été utilisée. (who.int)
  • If sufficient material exists, DNA will be extracted and PCR performed to provide a barcode sequence that will include the internal transcribed spacer regions and the 28S nuclear ribosomal DNA regions when possible and when not available from other sources. (usda.gov)
  • A) Ribosomal internal transcribed spacer 1-based phylogeny. (cdc.gov)
  • 2004. An expanded plastid DNA phylogeny of Orchidaceae and analysis of jacknife branch support strategy. (people.su)
  • These primers amplify approximately 600 basepairs of the ITS1-5.8S-ITS2 region of the ribosomal cistron. (cdc.gov)
  • These primers amplify approximately 620 basepairs of the 28S region of the ribosomal cistron. (cdc.gov)
  • These primers amplify a section of the intergenic spacer in the ribosomal cistron. (cdc.gov)
  • Reslit 1229 - Phylogenetic relationships in Helianthus (Asteraceae) based on nuclear ribosomal DNA internal transcribed spacer region sequence data (1998) E. E. Schilling, C. R. Linder, R. D. Noyes and L. (wildflower.org)
  • 2000. A phylogenetic analysis of Laeliinae (Orchidaceae) based on sequence data from internal transcribed spacers (ITS) of nuclear ribosomal DNA. (people.su)
  • 2005. An overview of the phylogenetic relationships within Epidendroideae inferred from multiple DNA regions and recircumscription of Epidendreae & Arethuseae (Orchidaceae). (people.su)
  • The spatial distribution of variable haplotypes from the nuclear ribosomal DNA internal transcribed spacer (ITS1-5.8S-ITS2) region and a ribosomal DNA group-I intron from the obligate, rocky shore intertidal red alga Porphyra umbilicalis were used to reconstruct its biogeographical history since the last glacial maximum in the North Atlantic. (degruyter.com)
  • Polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis of the ribosomal RNA gene internal transcribed spacer-1 (ITS1) was used. (who.int)
  • We performed internal transcribed spacer 1 (ITS1) ribosomal DNA sequencing analysis. (bvsalud.org)
  • The genetic variability of the isolates was first assessed using Random Amplified Polymorphic DNA (RAPD) markers. (bvsalud.org)
  • RAPD (Random Amplified Polymorphic DNA) markers have been most widely used for this purpose 8-10 . (bvsalud.org)
  • The result is a complex banding pattern that provides a community-specific profile, with each DNA band corresponding to a bacterial population on the original assemblage. (wikipedia.org)
  • Additional identification of M. titans includes sequencing a region of the mushroom's DNA (DeLong and Brewer 2013). (ufl.edu)
  • Molecular identity was determined by PCR and sequencing of the ITS-2 region of the nuclear ribosomal DNA, and compared to other protostrongylids. (tamu.edu)
  • 2002. Molecular phylogenetics of Cymbidium (Orchidaceae: Maxillarieae): sequence data from Internal Transcribed Spacers (ITS) of nuclear ribosomal DNA and plastid matK. (people.su)
  • The project started in April 2006, and collection of DNA sequence data was completed in 2011. (berkeley.edu)
  • A total of 156 clear and reproducible DNA fragments were amplified by 24 RAPD primers, among which 154 fragments (98.72%) were found to be polymorphic. (degruyter.com)
  • Foi usado um total de 78 primers RAPD, sendo que 8 apresentaram 54% de fragmentos de DNA polimórficos. (scielo.br)
  • 3% difference in the ribosomal DNA Internal Transcribed Spacers (ITS) and 8.6% variation in the more rapidly evolving mitochondrial cytochrome c oxidase I (COI) gene. (nottingham.ac.uk)
  • Molecular and structural organization of the non-transcribed spacer (NTS) of ribosomal DNA of D. melanogaster. (cnr.it)
  • This RD-PCR proved highly sensitive, detecting a single larval stage and as little as 0.78 ng of genomic DNA (gDNA) from an adult schistosome, providing a cost-effective, rapid and robust molecular tool for high-throughput screening of S. haematobium and S. bovis populations. (imperial.ac.uk)
  • A caracterização molecular foi baseada em marcadores RAPD, sequenciamento da região do espaçador interno transcrito do DNA ribossomal e "fingerprinting" telomérico. (scielo.br)
  • All 3 trees were prepared by using DNA sequence alignments and PHYLIP version 3.67 ( http://evolution.genetics.washington.edu/phylip.html ). (cdc.gov)
  • Meta-genomic sequencing for total DNA isolated from samples. (forsyth.org)
  • In this review, we recount the history of DNA-based methods for identification of botanicals and discuss some of the difficulties in defining a specific bar code or codes to use. (thieme-connect.com)
  • In recent years, several candidate DNA regions, such as matK , rbcL , psbA-trnH, and ITS/ITS2, have been assessed for their potential as DNA barcodes in plants [ 21 - 25 ]. (hindawi.com)
  • The aim of this study was to develop, refine and evaluate an alternative methodology, which enables better preservation of large numbers of individual schistosome larval stages and eggs collected in low resource endemic areas, to provide PCR-quality DNA for multi-locus genetic analysis. (imperial.ac.uk)
  • Saccharomyces cerevisiae deficient in the early anaphase release of Cdc14 can traverse anaphase I without ribosomal DNA disjunction and successfully complete meiosis. (wroc.pl)
  • Pour identifier les espèces de Leishmania, des techniques moléculaires ont été appliquées sur des échantillons prélevés chez 64 patients atteints de leishmaniose cutanée orientés vers l'hôpital régional d'Herat en 2013. (who.int)
  • 2001. Experimental hybridization reveals biased inheritance of the internal transcribed spacer of the nuclear ribosomal DNA in Begonia ×taipeiensis . (efloras.org)
  • DNA studies have shown that, despite not having a twisted fruit, African violets evolved from within the Tanzanian Streptocarpus subgenus Streptocarpella . (wikipedia.org)
  • Construction and validation of customized genomes for human and mouse ribosomal DNA mapping. (wroc.pl)
  • Recently, DNA-based methods have been applied to these products because DNA is not changed by growth conditions unlike the chemical constituents of many active pharmaceutical agents. (thieme-connect.com)
  • Scrophularia neesii und S. umbrosa ließen sich durch relative Messung des DNA-Gewichtes mit Durchflusszytometrie sicher unterscheiden. (researchgate.net)
  • The relative abundances of its DNA and RNA reads were positively associated with soil carbon and nitrogen concentrations and δ13C values, with the relative abundance of its DNA being negatively associated with soil pH value. (bas.ac.uk)
  • DNA-Based Authentication of Botanicals and Plant-Derived Dietary Supplements: Where Have We Been and Where Are We Going? (thieme-connect.com)