• Eukaryotes
  • RNA polymerase 1 (also known as Pol I) is, in higher eukaryotes, the polymerase that only transcribes ribosomal RNA (but not 5S rRNA, which is synthesized by RNA polymerase III), a type of RNA that accounts for over 50% of the total RNA synthesized in a cell. (wikipedia.org)
  • Restriction
  • Overnight digestion (10-16 hours) of about 300-500 ng of amplicon DNA in a 20 μL system with 4-5 units of Restriction Enzyme along with the recommended buffer at the prescribed temperature is recommended. (wikipedia.org)
  • Objective: The present study was conducted to evaluate the efficacy of restriction fragment length polymorphism (RFLP) analysis of PCR amplified ribosomal (r) DNA including internal transcribed spacers (ITS), as an identification tool. (aber.ac.uk)
  • restriction site mapping of chloroplast DNA. (springer.com)
  • Genome
  • The double FISH analysis of two repetitive DNAs (a satellite DNA and ribosomal DNA) in 12 natural populations of the grasshopper Eyprepocnemis plorans collected at the south (Granada and Málaga provinces) and south-east (Albacete and Murcia provinces) of the Iberian Peninsula has shown their widespread presence throughout the whole genome as well as extensive variation among populations. (springer.com)
  • Ribosomal DNA clusters and telomeric repeats are important parts of eukaryotic genome. (telomerescience.com)
  • bacterial
  • Broad-range sequencing of bacterial 16S ribosomal DNA represents an alternative approach for establishing the underlying organism in IE. (biomedcentral.com)
  • She has conducted post-doctoral research on bacteriophage genetics at Stanford University, bacterial DNA replication at the Boston Biomedical Research Institute and studied axenic cultivation at the National Institutes of Health. (wikipedia.org)
  • evolutionary
  • We discuss evolutionary trends and possible mechanisms of changes in the number of ribosomal clusters. (telomerescience.com)
  • This article is from Systematic Biology, volume 63.AbstractThe ribosomal RNA encapsulates a wealth of evolutionary information, including genetic variation that can be used to discriminate between organisms at a wide range of taxonomic levels. (duhnnae.com)
  • hybridization
  • This was confirmed by Sibley and Ahlquist (1990) based on DNA-DNA hybridization and subsequently by Paton et al. (wikipedia.org)
  • Sibley and Ahlquist furthermore removed button-quails (Turnicidae) from the Gruifomes based on large DNA-DNA hybridization distances to other supposed Gruiformes. (wikipedia.org)
  • polymerase
  • Myc is known to bind to human ribosomal DNA in order to stimulate rRNA transcription by RNA polymerase I. Two specific mechanisms have been identified, ensuring proper control of rRNA synthesis and Pol I-mediated transcription. (wikipedia.org)
  • extraction
  • Results: The banding profiles were observed about 8 h from initiating DNA extraction. (aber.ac.uk)
  • Precautions taken to avoid laboratory contamination included physical separation of the pre-PCR areas used for sample preparation, DNA extraction, and preparation of the reaction mixtures from the post-PCR areas. (alliedacademies.org)
  • isolates
  • Methods: Total cellular DNA was extracted from 26 Japanese isolates of T. tonsurans, along with several taxa of the members in the T. mentagrophytes complex, T. rubrum, T. violaceum and Epidermophyton floccosum, using a mini-preparation method. (aber.ac.uk)
  • isolation
  • DNA was then extracted using NucleoSpin Tissue isolation kit (CLONTECH Laboratories Inc., Palo Alto, Calif.) according to the manufacturer's instructions. (alliedacademies.org)