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  • Genome
  • Complementary DNA is usually used to cloned eukaryotic genes in prokaryotes to express specific proteins and is naturally produced by retrovirus like HIV-1 which able to incorporate to the host genome where it creates a provirus. (biology-online.org)
  • If the ratio of approximately 20 DNA sequence variants per gene holds over the entire genome (with approximately 20,000 genes) that means that every person who elects to have their genome sequenced will be provided with almost half a million Variants of Unknown Significance. (wikipedia.org)
  • As a post-doctoral fellow in David Baltimore's laboratory at MIT (1979-1982), Racaniello used recombinant DNA technology to clone and sequence the genome of the small RNA animal virus poliovirus. (wikipedia.org)
  • The information content of an organism is recorded in the DNA of its genome and expressed through transcription. (wikipedia.org)
  • genes
  • Microarrays prepared by high-speed robotic printing of complementary DNAs on glass were used for quantitative expression measurements of the corresponding genes. (nih.gov)
  • gene
  • During the late 1970s, Aviv entered the field of Recombinant DNA (genetic engineering) and applied research, and produced a microorganism (E. coli) containing the gene for Bovine Growth Hormone. (wikipedia.org)
  • ESTs contain enough information to permit the design of precise probes for DNA microarrays that then can be used to determine the gene expression. (wikipedia.org)
  • sequences
  • Once amplified, the sequence can be cut at each end with nucleases and inserted into one of many small circular DNA sequences known as expression vectors. (wikipedia.org)
  • Thus, 3n7nz A ( 1 ) has the potential for use as a fluorescent probe for structural studies of DNAs/RNAs including the detection of single-base alterations in target DNA sequences. (rsc.org)
  • synthesize
  • To synthesize an additional DNA strand, traditionally one would digest the RNA of the hybrid strand, using an enzyme like RNase H, or through alkali digestion method. (wikipedia.org)
  • found
  • The researchers also found one of the herbal medicines for sale had trace amounts of snow leopard DNA in it. (blogspot.com)
  • In addition they found DNA from pit vipers, frogs and trace amounts of cat and dog DNA. (blogspot.com)
  • cell
  • We examined the cell proliferation effect and mechanism of C. concinna -derived cryptocaryone (CPC) on oral cancer cells in terms of cell viability, apoptosis, reactive oxygen species (ROS), mitochondrial depolarization, and DNA damage. (biomedcentral.com)
  • CPC-induced cell responses in terms of cell viability, apoptosis, oxidative stress, and DNA damage were rescued by N-acetylcysteine pretreatment, suggesting that oxidative stress plays an important role in CPC-induced death of oral cancer cells. (biomedcentral.com)
  • alternative
  • The multi-tiered approach outlined in this study provides a much-needed auditing toolkit that should swiftly form the basis of best-practice pharmacovigilance across the CAM [complementary and alternative medicines] sector. (blogspot.com)
  • host
  • Such vectors allow for self-replication, inside the cells, and potentially integration in the host DNA. (wikipedia.org)
  • seen
  • That is, a difference can be seen in the DNA sequence, but the differences have no effect on the growth or health of the person. (wikipedia.org)