As a result of the inhibition of actin polymerization, cytochalasins can change cellular morphology, inhibit cellular processes such as cell division, and even cause cells to undergo apoptosis. (wikipedia.org)
Cytochalasins can also have an effect on other aspects of biological processes unrelated to actin polymerization. (wikipedia.org)
Actin Polymerization - The Mechanism of Action of Cytochalasin D" (PDF). (wikipedia.org)
Cytochalasins are a group of about 20 known mycotoxins, which affect actin stress fibers, causing actin depolymerization.Cytochalasin E Inhibits actin polymerization in blood platelets. (fermentek.com)
Furthermore, cytokine secretion was reduced following treatment with the antioxidant, N-acetylcysteine, and cytochalasin D, an inhibitor of actin polymerization. (cdc.gov)
Inhibitor1
Cytochalasin B, an inhibitor of actin microfilaments, was employed during in vitro maturation to inhibit first polar body emission or during parthenogenetic activation to block second polar body emission. (techscience.com)
Inhibits3
For example, cytochalasin A and cytochalasin B can also inhibit the transport of monosaccharides across the cell membrane, cytochalasin H has been found to regulate plant growth, cytochalasin D inhibits protein synthesis and cytochalasin E prevents angiogenesis. (wikipedia.org)
This test is based on the principle that cytochalasin D very effectively inhibits platelet function by inhibition of the contractile elements. (wikipedia.org)
Cytochalasin E, an Epoxide Containing Aspergillus-Derived Fungal Metabolite, Inhibits Angiogenesis and Tumor Growth" (PDF). (wikipedia.org)
Microfilaments2
Cytochalasins are known to bind to the barbed, fast growing plus ends of microfilaments, which then blocks both the assembly and disassembly of individual actin monomers from the bound end. (wikipedia.org)
Effects of Cytochalasin and Phalloidin on Actin" (PDF). (wikipedia.org)
Cytochalasin D is able to mimic the effects of phalloidin on the rat liver. (mpg.de)
Disrupt1
During time-lapse image, fresh media buffered with HEPES was continuously perfused through the dish and temperature was maintained at 37°C. After 1-2 hours, cells were treated with cytochalasin D (to disrupt f-actin) and/or nocodazole (to depolymerize microtubules). (arvojournals.org)
Inhibition1
Mechanism of inhibition of human glucose transporter GLUT1 is conserved between cytochalasin B and phenylalanine amides. (ucsf.edu)
Vitro3
A correct IZUMO1 repositioning following in vitro induction of F-actin depolymerization, by cytochalasin D treatment, occurred. (ijbs.com)
In experiment 1, the effect of cytochalasin B on in vitro maturation of bovine oocytes was evaluated. (techscience.com)
In experiment 2, development of embryos exposed to cytochalasin B during in vitro maturation (CytoB-IVM) or during activation (CytoB-ACT) was compared. (techscience.com)
Effects2
The effects of many different cytochalasins on actin filaments were analyzed and higher concentrations (2-20 μM) of CD were found to be needed to remove stress fibers. (wikipedia.org)
This work on formulation, tolerated doses iji vivo, and localized peritu-moral effects of CB now permits evaluation of systemic antitumor effects of cytochalasin B as a single agent. (syr.edu)
Cell1
Cytochalasins have the ability to permeate cell membranes, prevent cellular translocation and cause cells to enucleate. (wikipedia.org)
Chemical2
Due to their chemical nature, cytochalasins can help researchers understand the importance of actin in various biological processes. (wikipedia.org)
Cytochalasin B is a chemical that disrupts microfilament formation. (quizlet.com)
Application1
Cytochalasin has found practical application in thromboelastometry (TEM) whole blood assays for the assessment of fibrinogen and fibrin polymerization disorders in the FIBTEM assay on ROTEM. (wikipedia.org)
Actin filaments3
Cytochalasins are fungal metabolites that have the ability to bind to actin filaments and block polymerization and the elongation of actin. (wikipedia.org)
The effects of many different cytochalasins on actin filaments were analyzed and higher concentrations (2-20 μM) of CD were found to be needed to remove stress fibers. (wikipedia.org)
Perturbation of actin filaments by either cytochalasin-D or conditional Cofilin expression resulted in decreased synaptic OXT levels. (zfin.org)
Macrophages1
C ) Adherence of bacterial cells to RAW264.7 macrophages following treatment with 10 µM cytochalasin D to inhibit phagocytic uptake. (elifesciences.org)
Angiogenesis2
For example, cytochalasin A and cytochalasin B can also inhibit the transport of monosaccharides across the cell membrane, cytochalasin H has been found to regulate plant growth, cytochalasin D inhibits protein synthesis and cytochalasin E prevents angiogenesis. (wikipedia.org)
Cytochalasin E, an Epoxide Containing Aspergillus-Derived Fungal Metabolite, Inhibits Angiogenesis and Tumor Growth" (PDF). (wikipedia.org)
Inhibit2
As a result of the inhibition of actin polymerization, cytochalasins can change cellular morphology, inhibit cellular processes such as cell division, and even cause cells to undergo apoptosis. (wikipedia.org)
Pretreatment with either Mab13 or Cytochalasin‐D, to inhibit β‐integrin or actin polymerization, respectively, significantly reduced fibrin and stretch‐induced FN fibril assembly. (lww.com)
Cell membrane1
In addition, the Love-wave sensor, which can sense binding events within the relatively short distance of approximately 50 nm from the device surface, was sensitive to the number of bonds formed between the cell membrane and the device surface while the QCM-D, which can sense deeper within the liquid, was found to respond well to stimuli that affected the cell membrane rigidity (cytochalasin D treatment). (unboundmedicine.com)
Binds2
The results are interpreted in terms of the nucleation-elongation model for actin polymerization and the supposition that cytochalasin binds preferentially to one end of a growing polymer. (wustl.edu)
Cytochalasin B, a generic glucose transporter inhibitor, reduced insulin-stimulated glucose uptake by podocytes to basal levels, showing the process to be mediated by glucose transporters (data not shown). (medscape.com)
Cytoskeletal3
The use of cytochalasins has been so important to understanding cytoskeletal movement and many other biological processes, researchers have created two synthetic cytochalasins. (wikipedia.org)
Cytochalasin A Cytochalasin B Cytochalasin C Cytochalasin D Cytochalasin E Cytochalasin F Cytochalasin H Cytochalasin J Cytoskeletal drugs Haidle, A. M. (wikipedia.org)
This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. (biolegend.com)
Prevents1
Cytochalasin B prevents both migration and wound-associated replication when applied to wounded monolayers of bovine endothelium in tissue culture. (nih.gov)
Protein1
We have isolated an actin binding protein from platelets that shows effects similar to those of cytochalasin D. This protein appears to correspond to macrophage gelsolin. (wustl.edu)
Embryos1
The timing mechanism of rRNA gene expression was studied in Xenopus laevis embryos whose cleavage was arrested by treatment with cytochalasin B or podophyllotoxin, and in fertilized eggs from which about 40% of the cytoplasm was extracted by micromanipulation. (elsevierpure.com)
The treatment of strontium chloride (SrCl2) combined with cytochalasin B for 4 h produced the highest heterozygous diploid rate and blastulation rate. (tubitak.gov.tr)
These micronucleated cells have been enucleated by combining centrifugation and cytochalasin B treatment, and the resultant microcells have been purified and fused to recipient mouse (LMTK-) cells. (nih.gov)
Cells4
Cytochalasins have the ability to permeate cell membranes, prevent cellular translocation and cause cells to enucleate. (wikipedia.org)
The normal low background rate of replication in undisturbed areas of the cultures is not inhibited by cytochalasin B. These results suggest that some form of movement may be required for initiation of wound-associated replication by endothelial cells. (nih.gov)
Gomzikova M.O, Aimaletdinov A.M, Bondar O.V, Immunosuppressive properties of cytochalasin B-induced membrane vesicles of mesenchymal stem cells: comparing with extracellular vesicles derived from mesenchymal stem cells//Scientific Reports. (kpfu.ru)
The cells were then treated with 3 micrograms per milliliter cytochalasin-B (CytB). (cdc.gov)
Formation4
Studies done with cytochalasin D (CD) have found that CD-actin dimers contain ATP-bound actin upon formation. (wikipedia.org)
Cytochalasin D and Platelet Gelsolin Accelerate Actin Polymer Formation. (wustl.edu)
However, the presence of cytochalasin D decreases the extent of polymer formation. (wustl.edu)
Dive into the research topics of 'Cytochalasin D and Platelet Gelsolin Accelerate Actin Polymer Formation. (wustl.edu)
Initial1
The initial bacterial load was set to 100% (black, technical replicates) while the bacterial uptake without cytochalasin D is shown as a control. (elifesciences.org)