• mRNA
  • An inhibitor of mRNA translation, cycloheximide, reduced RNA degradation by hepatic microsomal ribonuclease in a dose-related manner. (aspetjournals.org)
  • Effects of cycloheximide on gene expression changes in DPCs induced by oleuropein treatment.DPCs were allowed to attach overnight and then treated (60 min) with cycloheximide (10 μg/ml) and thereafter switched to medium with or without oleuroepin (20 μM) in the presence of cycloheximide (10 μg/ml) for 24 h. mRNA expression levels of LEF1, Cyc-D1, IGF-1, HGF, VEGF, and KGF were measured by RT-PCR. (nih.gov)
  • activity
  • Graphical analysis of the time plot of ribonuclease activity decay following cycloheximide administration indicated that the half-life of the microsomal ribonuclease was 54 min. (aspetjournals.org)
  • The decrease in cdc2/cyclin B kinase activity, however, does not seem to be required for the decrease in MAP kinase activity, since the decrease in MAP kinase activity still occurs in cycloheximide-treated eggs that are also incubated in the presence of nocodazole, which inhibits cyclin B degradation and hence the decrease in cdc2/cyclin B kinase. (biologists.org)
  • Effects
  • Due to significant toxic side effects, including DNA damage, teratogenesis, and other reproductive effects (including birth defects and toxicity to sperm), cycloheximide is generally used only in in vitro research applications, and is not suitable for human use as a therapeutic compound. (wikipedia.org)
  • A) Western blot demonstrating cycloheximide effects of Wnt-3A mediated accumulation of β-catenin. (nih.gov)
  • treatment
  • Cycloheximide treatment provides the ability to observe the half-life of a protein without confounding contributions from transcription or translation. (wikipedia.org)
  • Cycloheximide treatment of metaphase II-arrested mouse eggs also results in resumption of meiosis but bypasses the fertilization-induced Ca2+ transient. (biologists.org)
  • However, it is not known if cycloheximide treatment results in the same temporal changes in cdc2/cyclin B kinase and MAP kinase activities that are intimately associated with resumption of meiosis. (biologists.org)
  • different
  • Cycloheximide Export data can be useful in different kind of analysis such as: Export price, Quantity, market scenarios, Price trends, Duty optimization and many more. (eximpulse.com)
  • results
  • The results indicate that the short onset of microsomal RNase reduction produced by cycloheximide may be due to a more rapid turnover of this enzyme in comparison with other microsomal proteins. (aspetjournals.org)
  • Our results showed that the accumulation of β-catenin by oleuropein, as determined by immunocytochemistry, was blocked by cycloheximide (Fig 7). (nih.gov)
  • changes
  • We report that cycloheximide-treated mouse eggs manifest similar temporal changes in the decrease in both cdc2/cyclin B kinase and MAP kinase activities that occur following fertilization, although cortical granule exocytosis is not stimulated. (biologists.org)