• deletions
  • In p53 mutated breast cancers high cyclin E content was associated with insertions, deletions and nonsense point mutations in the p53 tumor suppressor gene, whereas low cyclin E was linked to p53 missense point mutations. (diva-portal.org)
  • Transient transfections using CAT (chloramphenicol acetyltransferase) reporter constructs containing sequential deletions of the cyclin D3 promoter defined positively and negatively regulated regions. (bl.uk)
  • gene
  • I have been defining the cis -acting elements and trans -acting factors that control transcription of the human cyclin D3 gene in T-cells. (bl.uk)
  • Genomic clones for the human cyclin D3 gene, isolated from a human chromosome 6 library, were analysed by restriction endonuclease digestion and a sub-clone extending 1.7kb upstream of exon 1 was sequenced and studied. (bl.uk)
  • The human cyclin D3 gene has a TATA-less promoter and a single dominant initiation site. (bl.uk)
  • A number of small molecules, including phenothiazines, were found that induced a gene signature in breast cancer cell lines opposite to that found in tamoxifen resistant vs. sensitive tumors and the ability of phenothiazines to down-regulate cyclin E2 and inhibit proliferation of tamoxifen resistant breast cancer cells was validated. (duhnnae.com)
  • protein
  • Ohtsubo M, Theodoras AM, Schumacher J, Roberts JM, Pagano M. Human cyclin E, a nuclear protein essential for the G1-to-S phase transition. (springer.com)
  • Fractionation of whole cell lysates by gel filtration chromatography revealed that approximately 90% of the cyclin E protein was present in inactive complexes containing the CDK inhibitors p21 and p27. (garvan.org.au)
  • Sequence
  • The minimal cyclin D3 promoter sequence was identified as a region 173bp upstream of the transcription initiation site. (bl.uk)
  • growth
  • The treatment of quiescent cells with growth factors results in the transcriptional activation of the D-type cyclin genes during G1. (bl.uk)
  • Comparison of these results with those for the cyclin D1 and D2 genes should elucidate how transcription of these genes is co-ordinately regulated by growth factors. (bl.uk)