• The system consists of a fixation reagent (PAXgene Tissue FIX), a stabilization reagent (PAXgene Tissue STABILIZER), prefilled containers for tissue collection, storage, and transportation, and kits for purification of RNA, DNA, or total RNA, including miRNA. (qiagen.com)
  • Hetero-bifunctional Reagents is a group of reagents that have an NHS ester (OSu) group that reacts with a primary amino group and a maleimide group that reacts with a thiol (SH group). (dojindo.com)
  • Homo-bifunctional Reagents are a group of reagents with two NHS esters. (dojindo.com)
  • The present invention relates to the field of bioconjugate preparation, and more particularly, to a class of bifunctional boronic compound complexing reagents useful for the conjugation of biological macromolecules, and the method of making and using such reagents. (justia.com)
  • Finally, amine reactive heterobifunctional cross-linkers can be used to introduce other functional groups for coupling ligands. (gbiosciences.com)
  • One type of cause is purely spontaneous, as reactive sugar molecules or oxidized fats in the blood react with one strand of collagen, which after a series of further rearrangements then binds to a neighboring collagen strand, crosslinking them together. (sens.org)
  • This approach involves the intramolecular cross-linking of 5′ amine- and 3′ disulfide-modified MO oligonucleotides using appropriately functionalized tethers, generating macrocyclic structures that conformationally resist RNA hybridization. (cdc.gov)
  • confers greater solubility to the crosslinker and linked proteins compared to crosslinkers having only hydrocarbon spacers. (thomassci.com)
  • Fixatives that contain formaldehyde cross-link biomolecules and modify nucleic acids and proteins. (qiagen.com)
  • CLIP is an antibody-based technique used to study RNA-protein interactions related to RNA immunoprecipitation (RIP) but differs from RIP in the use of UV radiation to cross-link RNA-binding proteins to the RNA that they are bound to. (abcam.com)
  • Optional 4-thiouridine pre-incubation and UV-A crosslinking could be used for certain proteins. (abcam.com)
  • 4-thiouridine enhances crosslinking of some proteins. (abcam.com)
  • In addition, because formaldehyde efficiently crosslinks protein to DNA as well as protein to protein, this method can, in principle, detect proteins that bind DNA directly as well as those that bind DNA indirectly through other factors. (sigmaaldrich.com)
  • Crosslinks act like molecular "handcuffs," taking two previously independent neighboring proteins and binding them together, impairing their function similarly to how tying together participants' legs in a three-legged race makes it a struggle to get to the finish line. (sens.org)
  • Other kinds of crosslinks are formed intentionally by enzymes whose proper job is to shape and reshape the functional properties of proteins in response to other age-related changes - but such crosslinks can sometimes serve short-term needs, while becoming dysfunctional as they accumulate excessively over time. (sens.org)
  • In addition to chemical damage in the form of crosslinks, structural proteins also suffer mechanical damage, resulting from the same kinds of stresses and strains that cause automobile and airplane parts to distort or to shear. (sens.org)
  • In order to develop zein fibers with improved physical properties and solvent resistance, formaldehyde was used as the cross-linking reagent before spinning. (usda.gov)
  • The formaldehyde cross-linked zein fabrics had double the tensile strength of control fibers. (usda.gov)
  • When tri(ethylene glycol) alone was used as the plasticizer, the cross-linking reaction did not occur as readily. (usda.gov)
  • During tissue fixation, storage, and processing, cross-links lead to degradation of nucleic acids. (qiagen.com)
  • The fixation and stabilization method preserves tissue morphology and the integrity of nucleic acids without destructive cross-linking and degradation found in formalin-fixed tissues. (qiagen.com)
  • Fixation is comparable to formalin fixation, but without the destructive nucleic acid crosslinking and degradation. (qiagen.com)
  • Such crosslinks lead to nucleic acid degradation during tissue fixation, storage, and processing. (qiagen.com)
  • PAXgene Tissue reagents in prefilled containers and PAXgene Tissue Kits provide a complete preanalytical solution for collection, fixation, and stabilization of tissue, and purification of high-quality nucleic acids for molecular research analysis. (qiagen.com)
  • IHC antigen retrieval reagent (citrate, pH 7.0) is used to recover antigens masked by fixation in cross linking fixatives such as formalin. (enzolifesciences.com)
  • Storage: -20C Application: TRUEPEG is used to crosslink PVA and dextran polymers to generate stable hydrogel. (thomassci.com)
  • The Avidin-Biotin system has been utilized extensively for enzyme-linked immuno solid-phase assay (ELISA), in which an enzyme-Avidin conjugate (useful for detection by reaction with the enzyme's substrate to afford a colored or chemiluminescent product) is employed to detect the presence of a biotinylated antibody, after first binding the antibody to an immobilized antigen or hapten. (justia.com)
  • This additional cross-linking step prevents contamination of the purified antigen with antibody. (gbiosciences.com)
  • In agglutination tests (eg, latex agglutination, coaggregation), very small particles (latex beads, gelatin particles, bacteria) are coupled to a reagent antigen or antibody. (msdmanuals.com)
  • if the target antibody or antigen is present in the specimen, it cross-links the particles, producing measurable agglutination. (msdmanuals.com)
  • A reagent that forms a bond to its reaction partner (the electrophile) by donating both bonding electrons. (mcw.edu)
  • In order to reduce the impact of moisture on physical properties of articles based on zein, the use of various reagents to induce cross linking were evaluated on a torque rheometer. (usda.gov)
  • EOS9.1 antibody can induce apoptosis without cross-linking. (biolegend.com)
  • To evaluate 1-year outcomes of corneal collagen crosslinking (CXL) for treatment of keratoconus and corneal ectasia. (nih.gov)
  • Collagen crosslinking was performed in eyes with keratoconus or ectasia. (nih.gov)
  • Collagen crosslinking was effective in improving UDVA, CDVA, the maximum K value, and the average K value. (nih.gov)
  • The main threat posed by crosslinking in the artery wall is to the strands of the protein collagen that run parallel to each other around the circumference of the vessel. (sens.org)
  • As more and more strands of collagen become crosslinked together over time, the blood vessels become ever more rigid, leading to a gradual rise in systolic blood pressure with age and all its deadly consequences. (sens.org)
  • In any mycobacteriology laboratory, cross-contamination, mislabeling errors, specimen mix-ups, and use of nonsterile reagents can cause the inadvertent transfer of bacilli from one specimen or culture to another specimen or culture. (cdc.gov)
  • immunochemical staining of dermal tissue and enzyme-linked immunoassays (ELISA). (cdc.gov)
  • Our detailed protocol and tips for carrying out UV cross-linking and immunoprecipitation (CLIP). (abcam.com)
  • EZ-Zyme™ Chromatin Prep Kit Contains proprietary reagents optimized for the enzymatic shearing of chromatin from mammalian cells at higher resolution than sonication for use in chromatin immunoprecipitation (ChIP) assays. (sigmaaldrich.com)
  • The EZ-Zyme kit contains reagents optimized for generating enzymatically processed chromatin from mammalian cells for use in chromatin immunoprecipitation. (sigmaaldrich.com)
  • G-Biosciences Cross-Linking Immunoprecipitation kit contains all the reagents necessary to complete all aspects of immunoprecipitation, with the exception of labeling. (gbiosciences.com)
  • Based on our Classical Immunoprecipitation kit , the Cross-Linking Immunoprecipitation kit incorporates the homobifunctional cross-linker DSS to covalently couple the antibody to the Immobilized Protein A/G resin . (gbiosciences.com)
  • PC Azide Agarose is a 6% crosslinked agarose resin that is activated with alkyne groups through a photocleavable linker. (vectorlabs.com)
  • A divalent reagent is a reagent having two functional groups and is widely used in immunoassays and protein research. (dojindo.com)
  • TotalSeq™-D reagents are designed to profile protein expression at single cell level. (biolegend.com)
  • A hybridoma that produces MDI-protein specific IgM mAbs, which also has good cross-reactivity to hexamethylene diisocyanate conjugated protein, has recently been isolated and cloned. (cdc.gov)
  • Application: EncapGel(TM)-Cx Kit is a ready-to-use, chemically crosslinked hydrogel kit for the encapsulation and delivery of drugs, biomolecules, and cells. (thomassci.com)
  • Although all these studies strongly suggest that TEs may play a role in gene regulation through different molecular mechanisms, detailed analyses that link changes in expression with fitness effects are needed to conclude that TEs have a functional impact on gene expression. (nature.com)
  • One prominent form of molecular ECM damage is crosslinking. (sens.org)
  • PAXgene Tissue Containers are dual-chamber containers prefilled with 2 reagents. (qiagen.com)
  • The soluble fibrin is cross-linked to the vessel walls by factor Xllla. (horiba.com)
  • When adjacent strands are crosslinked together, it prevents them from spreading apart from one another to accommodate the surge of blood sent coursing down the arterial tree by the pumping action of the heart. (sens.org)
  • Simply enter the mass of reagent and the target concentration and the calculator will determine the rest. (tocris.com)
  • Dojin Kagaku offers a variety of divalent reagents with different spacer chain lengths and functional groups, so select the one that best suits your needs. (dojindo.com)
  • Since cross-links can not be removed completely, the resulting chemical modifications can cause inhibition in sensitive downstream applications such as quantitative PCR or RT-PCR. (qiagen.com)
  • To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. (biolegend.com)
  • in vitro induction of apoptosis 3 (DX2 antibody is required to be cross-linked for effective induction of apoptosis) and immunohistochemical staining 4,5 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. (biolegend.com)
  • detection of gene probes, enzyme-linked immuno solid-phase assay, monoclonal antibody drug targeting and medical imaging. (justia.com)
  • The high- f junctions can provide load-bearing abilities similar to a covalent cross-link while retaining the ability to self-repair and concurrently confer stimuli-responsive properties arising from the high- f junction species. (aip.org)
  • When splitting this cross-linked fibrin, characteristic products called D-dimers are released. (horiba.com)
  • Photoactivatable Cross-linking Probes " has 4 results in Products. (rndsystems.com)
  • Linking to a non-federal website does not constitute an endorsement by CDC or any of its employees of the sponsors or the information and products presented on the website. (cdc.gov)
  • For the quantification of CRP, particles consisting of a polystyrene core and a hydrophilic shell are used in order to link anti-CRP antibodies covalently. (cdc.gov)
  • Enzyme immunoassays use antibodies linked to enzymes to detect antigens and to detect and quantify antibodies. (msdmanuals.com)
  • In addition, enzymatic shearing is compatible with non-crosslinked ChIP (native ChIP), which allows analysis of histone modifications not only on cultured cells, but also on freshly dissected and frozen tissues. (sigmaaldrich.com)
  • SDS-PAGE analysis clearly showed cross-linking has occurred. (usda.gov)
  • For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. (biolegend.com)
  • To view the papers in pdf format, click on the "PDF Full-text" link, and use the free Adobe Reader to open them. (mdpi.com)
  • This unique type of network configuration offers an alternative to traditional dual-junction networks consisting of covalent and reversible cross-links. (aip.org)
  • Depending on the technique and the reagents used, a positive direct antiglobulin test has been reported in 1:1000 to 1:14,000 blood donors and 1%-15% of hospital patients. (medscape.com)
  • SimpleChIP ® Rat GAPDH Promoter Primers were tested on DNA isolated from cross-linked cells using the SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. (cellsignal.com)
  • Knockout cells or tissue, as well as non-cross-linked cells, are good negative controls, while knockdown cells are not recommended. (abcam.com)
  • Yumizen G DDi 2, the universal reagent, liquid & ready to use for semi-auto and full-auto systems, performs a particle-enhanced immuno-turbidimetric test. (horiba.com)
  • Mice homozygous for a targeted null mutation are viable and fertile but exhibit increased sensitivity to the DNA interstrand cross-linking agent mitomycin C. (jax.org)
  • With suitable amounts of these reagents in the presence of water as a plasticizer, cross-linking occurred as evidenced by the very large increase in viscosity. (usda.gov)