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  • bacteria
  • Bacteria of the genus Acinetobacter are known to form intracellular inclusions of polyhydroxyalkanoates under certain environmental conditions (e.g. lack of elements such as phosphorus, nitrogen, or oxygen combined with an excessive supply of carbon sources). (wikipedia.org)
  • These media contain lactose which is usually fermented by lactose fermenting bacteria producing colonies that can be identified and characterised. (wikipedia.org)
  • The plate count method relies on bacteria growing a colony on a nutrient medium so that the colony becomes visible to the naked eye and the number of colonies on a plate can be counted. (wikipedia.org)
  • progenitor
  • Methylcellulose is a semisolid media additive that allows an investigator to stain (with diaminobenzidine reagent for hemoglobin) and then count individual colonies, each arising from a single plated progenitor that is at the CFU-e stage. (wikipedia.org)
  • hematopoietic
  • However, contradictory evidences demonstrate that inhibition of osteoclast activity by bisphosphonate does not impair HSPC mobilization in response to Granulocyte-Colony Stimulating Factor (G-CSF) treatment [ 1 , 10 ], suggesting that osteoclasts may only intervene in certain types of hematopoietic stresses. (hindawi.com)
  • formation
  • This method allowed identification of A. calcoaceticus-A. baumannii complex by the formation of smooth, rounded, mucoid colonies at 37 °C. Closely related species could not be differentiated and individual species such as A. baumannii and Acinetobacter genomic species 3 could not be positively identified phenotypically. (wikipedia.org)
  • reagents
  • If the assay involves addition of exogenous reactants (the reagents), then their quantities are kept fixed (or in excess) so that the quantity (and quality) of the target is the only limiting factor for the reaction/assay process, and the difference in the assay outcome is used to deduce the unknown quality or quantity of the target in question. (wikipedia.org)
  • species
  • This method can be enhanced by using indicator medium which changes colour when acid forming species are present and by including a tiny inverted tube called a Durham tube in each sample tube. (wikipedia.org)
  • laboratory
  • An assay is an investigative (analytic) procedure in laboratory medicine, pharmacology, environmental biology and molecular biology for qualitatively assessing or quantitatively measuring the presence, amount, or functional activity of a target entity (the analyte). (wikipedia.org)
  • Since the assay itself (the analytic step) gets much attention, steps that get less attention by the chain of users, i.e. the preanalytic and the post analytic steps, are often less stringently regulated and generally more prone to errors - e.g. preanalytic steps in medical laboratory assays may contribute to 32-75% of all lab errors. (wikipedia.org)
  • measurement
  • That's why we have developed products specifically designed to automate colony counting and inhibition zone measurement, to relieve you of the tedium involved with these repetitive manual tasks. (synbiosis.com)
  • The assay usually aims to measure an intensive property of the analyte and express it in the relevant measurement unit (e.g. molarity, density, functional activity in enzyme international units, degree of some effect in comparison to a standard, etc. (wikipedia.org)
  • tuberculosis
  • This form of tuberculosis is called multi-drug-resistant tuberculosis (MDR-TB) is rapidly on the rise globally. (wikipedia.org)
  • Frequently asked questions on Xpert MTB/RIF assay" Retrieved on 12 June 2012 "WHO endorses new rapid tuberculosis test" 8 December 2010. (wikipedia.org)
  • method
  • Additionally, plating is the slowest method of all: most microorganisms need at least 12 hours to form visible colonies. (wikipedia.org)
  • The tabled figures are for total aerobic plate count, cfu/ml at 30 °C (minimum 48 hours incubation) with colony count determined by the pour plate method according to ISO 6222(21) or spread plate method on yeast extract agar. (wikipedia.org)
  • growth
  • Growth on the AMA indicates a positive transformation assay and confirms the isolate as a member of the genus Acinetobacter. (wikipedia.org)
  • count
  • This group has published guidelines about the actions to be taken to limit the number of colony forming units (i.e., the "aerobic count") of micro-organisms per mL at 30 °C (minimum 48 hours incubation): Almost all natural water sources contain Legionella and their presence should not be taken as an indication of a problem. (wikipedia.org)
  • addition
  • In addition, the enumeration of colonies on agar plates can be greatly facilitated by using colony counters. (wikipedia.org)
  • In addition, none of the colonies containing the T12 genome was negative for speA, and therefore, the conclusion was drawn that all lysogens produced the toxin. (wikipedia.org)
  • acid
  • Clostridium butyricum is a strictly anaerobic endospore-forming Gram-positive butyric acid producing bacillus subsisting by means of fermentation using an intracellularly accumulated amylopectin-like α-polyglucan (granulose) as a substrate. (wikipedia.org)
  • The core principle behind microarrays is hybridization between two DNA strands, the property of complementary nucleic acid sequences to specifically pair with each other by forming hydrogen bonds between complementary nucleotide base pairs. (wikipedia.org)
  • thus
  • Thus reading of an assay may be quite noisy and may involve greater difficulties in interpretation than an accurate chemical titration. (wikipedia.org)
  • consists
  • The standard preparation as marketed by Miyarisan Pharmaceutical (Tokyo, Japan) consists of white, marked tablets each containing 0.35 × 106 colony forming units (CFU) of C. butyricum MIYAIRI 588 (as active agent). (wikipedia.org)
  • level
  • The other reliable identification test at genus level is chromosomal DNA transformation assay. (wikipedia.org)
  • Assays have become a routine part of modern medical, environmental, pharmaceutical, forensic and many other businesses at various scales from industrial to curbside or field level. (wikipedia.org)
  • target
  • To be effective, the dilution of the original sample must be arranged so that on average between 30 and 300 colonies of the target bacterium are grown. (wikipedia.org)
  • The measured entity is generally called the analyte, the measurand or the target of the assay. (wikipedia.org)
  • often
  • For the above reasons, the CFU-e assay, as inefficient and variable as it can often be, is still in use today. (wikipedia.org)
  • Such industrial scale assays as these are often done in well equipped laboratories and with automated organization of the procedure-from ordering an assay to pre-analytic sample processing (sample collection, necessary manipulations e.g. spinning for separation or other processes, aliquoting if necessary, storage, retrieval, pipetting/aspiration etc. (wikipedia.org)
  • mouse
  • Titanocene Y can be given in the mouse in high dosages and it shows generally mild toxicity in the form of diarrhea. (wikipedia.org)
  • process
  • Those assays that are very highly commercially demanded have been well investigated in research and development sectors of professional industries, undergone generations of development and sophistication, and become copyrighted intellectual properties via highly competitive process patenting. (wikipedia.org)
  • variety
  • Second generation ATP tests are specifically designed for water, wastewater and industrial applications where, for the most part, samples contain a variety of components that can interfere with the ATP assay. (wikipedia.org)
  • major
  • Its residues 22-31 help to stabilize a four-helix bundle which forms the major part of that interface, and residue 13 and 15 also contributing to the activating interface. (wikipedia.org)