• Cells
  • The growth of mesenchymal stem cells was monitored by colony formation unit fibroblast (CFU-F) assays. (haematologica.org)
  • Results Multicolor cell sorting and CFU-F assays showed that mesenchymal stem cells were ~90-fold enriched in the MSCA-1 + CD56 − fraction and ~180-fold in the MSCA-1 + CD56 + fraction. (haematologica.org)
  • Indication, Collection, and Laboratory Processing of Cytologic Samples -- The Cellular and Acellular Components of the Urinary Sediment -- The Cytologic Makeup of the Urinary Sediment According to the Collection Technique -- Cytologic Manifestations of Benign Disorders Affecting Cells of the Lower Urinary Tract -- Tumors and Related Conditions of the Bladder and Lower Urinary Tract -- Urine-Based Assays Complementing Cytologic Examination in the Detection of Urothelial Neoplasm. (stanford.edu)
  • A significant reduction was observed in colony forming efficiency, anchorage independence and growth rate of cells transfected with RNaseT2. (nih.gov)
  • B) A bar diagram showing average numbers of colonies for three independent experiments corresponding to cells and clones shown in part A. (C) Photograph of plates containing parent CSG2 cells, cells transfected with empty vector, negative transfer clone 5 and RNaseT2 expressing clones 3, 9 and 13. (nih.gov)
  • D) A bar diagram showing an average of colony counts of three independent experiments each for cells and clones shown in part C. (nih.gov)
  • RT-PCR and Western blot assays were performed to determine the expression of Bcl-xL mRNA and protein in CRC cells and normal human intestinal epithelial cell line. (nih.gov)
  • The migration and invasion of CRC cells was determined by wound-healing and tranwell invasion assays. (nih.gov)
  • Conditioned media derived from G2B-10A cells enhanced colony formation of R2-T1AS cells, whereas prior paraformaldehyde (PFA) fixation of G2B-10A cells abrogated this enhancement effect. (nih.gov)
  • To determine whether G2B-10A cells enhance the tumorigenic growth of co-injected R2-T1AS cells in vivo, we used the nude mouse xenograft assay. (nih.gov)
  • These results indicate that soluble factors secreted by G2B-10A cells play a less important role in promoting R2-T1AS tumorigenesis in vivo, and that additional components are operative in the nude mouse xenograft assay. (nih.gov)
  • Adipose tissue-derived MSCs had greater proliferative potential than bone marrow-derived MSCs, while no significantly difference in colony efficiency were observed between the two types of cells. (biomedcentral.com)
  • EpiCult-B media can assay for the presence of mammary precursor cells using either the mammary colony-forming cell (Ma-CFC) assay or 3D Matrigel-based cultures. (wikipedia.org)
  • Methylcellulose-based medium is widely used to detect and quantify hematopoietic progenitor cells in the colony-forming unit (CFU) assay. (wikipedia.org)
  • Stromal cells are connective tissue cells that form the supportive structure in which the functional cells of the tissue reside. (wikipedia.org)
  • An ex vivo assay for examining the clonogenic potential of multipotent marrow cells was later reported in the 1970s by Friedenstein and colleagues. (wikipedia.org)
  • In this assay system, stromal cells were referred to as colony-forming unit-fibroblasts (CFU-f). (wikipedia.org)
  • In microbiology, a colony-forming unit (CFU, cfu, Cfu) is a unit used to estimate the number of viable bacteria or fungal cells in a sample. (wikipedia.org)
  • Counting with colony-forming units requires culturing the microbes and counts only viable cells, in contrast with microscopic examination which counts all cells, living or dead. (wikipedia.org)
  • The visual appearance of a colony in a cell culture requires significant growth, and when counting colonies it is uncertain if the colony arose from one cell or a group of cells. (wikipedia.org)
  • The purpose of plate counting is to estimate the number of cells present based on their ability to give rise to colonies under specific conditions of nutrient medium, temperature and time. (wikipedia.org)
  • However, solitary cells are the exception in nature, and most likely the progenitor of the colony was a mass of cells deposited together. (wikipedia.org)
  • Furthermore, unlike for the pro-erythroblast and later stages of erythroid development, no truly reliable and unique positive flow-cytometric markers exist, though it is possible to use negative exclusion markers to deplete a cell population of other precursors and differentiated cells by cell sorting, thus greatly enriching it for the CFU-e activity (Prospective isolation and global gene expression analysis of the erythrocyte colony-forming unit, Terszowski G. et al. (wikipedia.org)
  • It is possible to see a small spectrum of hemoglobinization level and possibly cell size, indicating that some cells in the colony have achieved the end-stage faster than others. (wikipedia.org)
  • Cell number in a colony is important because pro-erythroblast stage is also Epo-responsive (expresses Epo receptor), but the proliferative capacity of these cells is not as high, thus yielding a colony with fewer than 8 cells. (wikipedia.org)
  • Cell counts within liquid media (such as blood, plasma, lymph, or laboratory rinsate) are usually expressed as a number of cells per unit of volume, thus expressing a concentration (for example, 5,000 cells per milliliter). (wikipedia.org)
  • If the cells are efficiently distributed on the plate, it can be generally assumed that each cell will give rise to a single colony or Colony Forming Unit (CFU). (wikipedia.org)
  • Although this method can be time consuming, it gives an accurate estimate of the number of viable cells (because only they will be able to grow and form visible colonies). (wikipedia.org)
  • It is therefore extensively used in experiments aiming to quantify the number of cells resisting drugs or other external conditions (for instance the Luria-Delbrück experiment or the gentamicin protection assay). (wikipedia.org)
  • On the other hand, older generation qualitative assays, especially bioassays, may be much more gross and less quantitative (e.g., counting death or dysfunction of an organism or cells in a population, or some descriptive change in some body part of a group of animals). (wikipedia.org)
  • agar
  • Moreover, two other models of benign MECs, MCF-12A and HuMECs, also enhanced R2-T1AS colony growth in soft agar and clonogenicity assays. (nih.gov)
  • Colony-forming units are used to quantify results in many microbiological plating and counting methods, including: The Pour Plate method wherein the sample is suspended in a petri dish using molten agar cooled to approximately 40-45 °C (just above the point of solidification to minimize heat-induced cell death). (wikipedia.org)
  • In addition, the enumeration of colonies on agar plates can be greatly facilitated by using colony counters. (wikipedia.org)
  • The tabled figures are for total aerobic plate count, cfu/ml at 30 °C (minimum 48 hours incubation) with colony count determined by the pour plate method according to ISO 6222(21) or spread plate method on yeast extract agar. (wikipedia.org)
  • In this assay, a naturally competent tryptophan auxotrophic mutant of Acinetobacter baylyi (BD4 trpE27) is transformed with the total DNA of a putative Acinetobacter isolate and the transformation mixture is plated on a brain heart infusion agar. (wikipedia.org)
  • progenitor
  • Methylcellulose is a semisolid media additive that allows an investigator to stain (with diaminobenzidine reagent for hemoglobin) and then count individual colonies, each arising from a single plated progenitor that is at the CFU-e stage. (wikipedia.org)
  • bacteria
  • Bacteria of the genus Acinetobacter are known to form intracellular inclusions of polyhydroxyalkanoates under certain environmental conditions (e.g. lack of elements such as phosphorus, nitrogen, or oxygen combined with an excessive supply of carbon sources). (wikipedia.org)
  • These media contain lactose which is usually fermented by lactose fermenting bacteria producing colonies that can be identified and characterised. (wikipedia.org)
  • The plate count method relies on bacteria growing a colony on a nutrient medium so that the colony becomes visible to the naked eye and the number of colonies on a plate can be counted. (wikipedia.org)
  • hematopoietic
  • However, contradictory evidences demonstrate that inhibition of osteoclast activity by bisphosphonate does not impair HSPC mobilization in response to Granulocyte-Colony Stimulating Factor (G-CSF) treatment [ 1 , 10 ], suggesting that osteoclasts may only intervene in certain types of hematopoietic stresses. (hindawi.com)
  • pharmacology
  • An assay is an investigative (analytic) procedure in laboratory medicine, pharmacology, environmental biology and molecular biology for qualitatively assessing or quantitatively measuring the presence, amount, or functional activity of a target entity (the analyte). (wikipedia.org)
  • formation
  • This method allowed identification of A. calcoaceticus-A. baumannii complex by the formation of smooth, rounded, mucoid colonies at 37 °C. Closely related species could not be differentiated and individual species such as A. baumannii and Acinetobacter genomic species 3 could not be positively identified phenotypically. (wikipedia.org)
  • bacterial
  • Bioaerosols have been revealed to cause certain human diseases, such as tuberculosis, Legionnaires' disease and different forms of bacterial pneumonia, coccidioidomycosis, influenza, measles, and gastrointestinal illness. (wikipedia.org)
  • In their experiment, every transformed, toxin-producing bacterial colony was lysogenic, i.e. contained the T12 gene. (wikipedia.org)
  • reagents
  • If the assay involves addition of exogenous reactants (the reagents), then their quantities are kept fixed (or in excess) so that the quantity (and quality) of the target is the only limiting factor for the reaction/assay process, and the difference in the assay outcome is used to deduce the unknown quality or quantity of the target in question. (wikipedia.org)
  • biological
  • But generally, assays involve biological material or phenomena which tend to be intrinsically more complex either in composition or in behavior or both. (wikipedia.org)
  • species
  • This method can be enhanced by using indicator medium which changes colour when acid forming species are present and by including a tiny inverted tube called a Durham tube in each sample tube. (wikipedia.org)
  • growth
  • Growth on the AMA indicates a positive transformation assay and confirms the isolate as a member of the genus Acinetobacter. (wikipedia.org)
  • significantly
  • Western blot assay showed that Ad/shBcl-xL could significantly induce activation of caspase-9, caspase-3 and PARP (Figure 7A). (nih.gov)
  • analysis
  • Some assays (e.g., biochemical assays) may be similar to or have overlap with chemical analysis and titration. (wikipedia.org)
  • test the quality of", from Anglo-French assaier, from assai (n.), from Old French essai "trial", and the noun assay thus means "trial, test of quality, test of character", mid-14th century, from Anglo-French assai and the meaning "analysis" is from the late 14th century. (wikipedia.org)
  • For assay of currency coins, this literally meant analysis of the purity of the gold or silver or whatever precious component was used to represent the true value of the coin. (wikipedia.org)
  • An assay (analysis) is never an isolated process and must be combined with pre- and post-analytic procedures. (wikipedia.org)
  • tuberculosis
  • This form of tuberculosis is called multi-drug-resistant tuberculosis (MDR-TB) is rapidly on the rise globally. (wikipedia.org)
  • Frequently asked questions on Xpert MTB/RIF assay" Retrieved on 12 June 2012 "WHO endorses new rapid tuberculosis test" 8 December 2010. (wikipedia.org)
  • addition
  • In addition, none of the colonies containing the T12 genome was negative for speA, and therefore, the conclusion was drawn that all lysogens produced the toxin. (wikipedia.org)
  • acid
  • Clostridium butyricum is a strictly anaerobic endospore-forming Gram-positive butyric acid producing bacillus subsisting by means of fermentation using an intracellularly accumulated amylopectin-like α-polyglucan (granulose) as a substrate. (wikipedia.org)
  • The core principle behind microarrays is hybridization between two DNA strands, the property of complementary nucleic acid sequences to specifically pair with each other by forming hydrogen bonds between complementary nucleotide base pairs. (wikipedia.org)
  • thus
  • Thus reading of an assay may be quite noisy and may involve greater difficulties in interpretation than an accurate chemical titration. (wikipedia.org)
  • consists
  • The standard preparation as marketed by Miyarisan Pharmaceutical (Tokyo, Japan) consists of white, marked tablets each containing 0.35 × 106 colony forming units (CFU) of C. butyricum MIYAIRI 588 (as active agent). (wikipedia.org)
  • usually
  • The assay usually aims to measure an intensive property of the analyte and express it in the relevant measurement unit (e.g. molarity, density, functional activity in enzyme international units, degree of some effect in comparison to a standard, etc. (wikipedia.org)
  • level
  • The other reliable identification test at genus level is chromosomal DNA transformation assay. (wikipedia.org)
  • Assays have become a routine part of modern medical, environmental, pharmaceutical, forensic and many other businesses at various scales from industrial to curbside or field level. (wikipedia.org)
  • group
  • This group has published guidelines about the actions to be taken to limit the number of colony forming units (i.e., the "aerobic count") of micro-organisms per mL at 30 °C (minimum 48 hours incubation): Almost all natural water sources contain Legionella and their presence should not be taken as an indication of a problem. (wikipedia.org)
  • target
  • To be effective, the dilution of the original sample must be arranged so that on average between 30 and 300 colonies of the target bacterium are grown. (wikipedia.org)
  • The measured entity is generally called the analyte, the measurand or the target of the assay. (wikipedia.org)
  • often
  • For the above reasons, the CFU-e assay, as inefficient and variable as it can often be, is still in use today. (wikipedia.org)
  • Such industrial scale assays as these are often done in well equipped laboratories and with automated organization of the procedure-from ordering an assay to pre-analytic sample processing (sample collection, necessary manipulations e.g. spinning for separation or other processes, aliquoting if necessary, storage, retrieval, pipetting/aspiration etc. (wikipedia.org)
  • Since the assay itself (the analytic step) gets much attention, steps that get less attention by the chain of users, i.e. the preanalytic and the post analytic steps, are often less stringently regulated and generally more prone to errors - e.g. preanalytic steps in medical laboratory assays may contribute to 32-75% of all lab errors. (wikipedia.org)
  • model
  • In a famous sermon, "A Model of Christian Charity," Winthrop described the new colony as "a City upon a Hill. (wikipedia.org)