• expression
  • OGX-011 is a 2′-methoxyethyl modified phosphorothioate antisense oligonucleotide that is complementary to clusterin mRNA and has been reported to inhibit clusterin expression and enhance drug efficacy in xenograft models. (oup.com)
  • The primary objective of this clinical study was to determine a biologically effective dose of OGX-011 that would inhibit clusterin expression in human cancer. (oup.com)
  • Prostate cancer tissue, lymph nodes, and serial samples of peripheral blood mononuclear cells were assessed for clusterin expression using quantitative real-time polymerase chain reaction and immunohistochemistry. (oup.com)
  • OGX-011 is well tolerated and reduces clusterin expression in primary prostate tumors. (oup.com)
  • Treatment with siRNA completely abolished cytoplasmic clusterin expression in all cell lines, but its down-regulation resulted in a significant decrease of cell growth only in the resistant line. (spandidos-publications.com)
  • Laure Debure and co-workers have examined the effects of clusterin expression in COS-7 cells (see p. 3109 ). (biologists.org)
  • The authors also observe, however, that clusterin expression disrupts mitochondria and induces apoptosis through the mitochondrial pathway - this can be prevented by expression of the pro-survival molecule Bcl-2 or inhibition of caspases. (biologists.org)
  • cells
  • Resistant cells were characterised by higher levels of cytoplasmic clusterin than sensitive cells, and antiestrogen treatments up-regulated clusterin levels in both sensitive and resistant cell lines. (spandidos-publications.com)
  • We therefore concluded that: i) basal clusterin levels are higher in antiestrogen resistant cells, ii) clusterin is up-regulated following antiestrogen treatment independently of the sensitivity of the cell line, iii) down-regulation of cytoplasmic clusterin restores sensitivity to toremifene in the antiestrogen-resistant cell line. (spandidos-publications.com)
  • response
  • The role of cytoplasmic clusterin in modulating response to two antiestrogens (toremifene and tamoxifen) was studied in two ER+ anti-estrogen-sensitive cell lines (MCF-7, 734B) and one ER+ antiestrogen-resistant cell line (T47D) using siRNA strategy. (spandidos-publications.com)