We employed a fluorophore-assisted light inactivation (FALI) technique that uses light irradiation to produce photogenerated singlet oxygen free radical damage that is directed at targeted proteins through chromophore-conjugated antibodies. (esmed.org)
Fluorophore-assisted light inactivation: a high-throughput tool for direct target validation of proteins. (esmed.org)
Laser Inactivation3
Selective destrcution of protein function by choromophore-sdisted laser inactivation. (esmed.org)
4）Jay DG, Keshishian H. Laser inactivation of fasciculin I disrupts axon adhesion of grasshopper pioneer neurons. (esmed.org)
9) Sano Y, Watanabe W, Matsunaga S. Choromophore-assisted laser inactivation-towards a spatiotemporal-functional analysis of proteins, and the ablation of chromatin, organelle and cell function. (esmed.org)
Neurons4
Through genetic engineering, proteins (or subunits) of interest are expressed in fusion with light-induced protein pumps to form a light-driven/repressed modular system that manipulates protein localization or oligomerization, as well as neuronal depolarization and hyperpolarization, to precisely and reversibly control the activation and repression of individual neurons. (drosophilidae.com)
Optogenetic techniques use genetically encoded light-gated ion channels to reversibly manipulate the excitability of neurons. (drosophilidae.com)
Optogenetics involves microbial rhodopsins (opsins) as light-sensitive actuators of neurons. (frontiersin.org)
impairing of early cancer c chromophore hydrolysis VIa neurons in cytoplasmic membrane during promoter. (evakoch.com)
Chromophore-Assisted Light Inactivation" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (uchicago.edu)
Growth cone1
Rescue of GAPDH using KillerRed fused to GAPDH followed by localized chromophore-assisted light inactivation of KillerRed-GAPDH in distal axons halts growth cone dynamics. (jneurosci.org)
Optogenetic3
As visible light is heavily scattered and absorbed by tissue, stimulating light for optogenetic control does not reach deep in the tissue irradiated from outside the subject body. (frontiersin.org)
Opsins widely used for optogenetic experiments are optimally activated by light in the visible spectrum (wavelength: ∼430-610 nm). (frontiersin.org)
Here we present a simple method addressing these needs, simultaneously increasing light sensitivity of an optogenetic switch while providing on-line detection of activation and relaxation. (nature.com)
Proteins3
Chromophore-asssted light inactivation of Halo Tag fusion proteins labeled with eosin in living cells. (esmed.org)
CD BioSciences offers a wide selection of photosensitive protein pumps (excitation and inhibition), custom transgenic Drosophila (e.g., nervous system) or cell lines, and downstream optical experimental analysis services based on your experimental characteristics (e.g., target proteins or signaling pathways, duration, color of light, site of action, etc. ). (drosophilidae.com)
Plastid-specific ribosomal proteins (PSRPs) have been proposed to play roles in the light-dependent regulation of chloroplast translation. (cipsm.de)
Photoreceptors1
Solving Blue Light Riddles: New Lessons from Flavin-binding LOV Photoreceptors. (mpg.de)
Specific1
Cancer progression is mainly driven by the genetic alterations in complex signaling pathways that leads to the activation of specific oncogenes and inactivation of tumor suppressor genes 9 - 12 . (ntno.org)
Optogenetics1
Microbial rhodopsins widely used for optogenetics are sensitive to light in the visible spectrum. (frontiersin.org)
Wavelength1
Upon exposure to light of the correct wavelength, the retinal chromophore bound to the opsin changes its chemical configuration, leading to a conformational change in the opsin structure. (frontiersin.org)
Channels1
Zhu L, Cui Q, Xiao H, Liao X, Chen X. Gating and inactivation of mechanosensitive channels of small conductance: A continuum mechanics study. (umassmed.edu)
Equipment1
2020). Light Modulation of Brain and Development of Relevant Equipment. (drosophilidae.com)
Leads2
Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis, but does not significantly affect cell proliferation in vivo: Ki-67 mutant mice developed normally and cells lacking Ki-67 proliferated efficiently. (wikipedia.org)
Rahmanzadeh R, Hüttmann G, Gerdes J, Scholzen T. Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis. (ijsurgery.com)
Blue light4
In order to apply the FALI technique to culture systems over a long period of time, we established a simple and easy-to-use long-term FALI (SELT-FALI) by modifying the original FALI to utilize weak blue light. (esmed.org)
Blue light levels commonly needed for actuation can be cytotoxic, precluding long-term experiments. (nature.com)
In situ-measured parameter values guide the design of target-inhibiting actuation trains with minimal blue-light exposure, and context-based optimisation can increase sensitivity and experimental throughput a further 10-fold without loss of temporal precision. (nature.com)
Blue-light generates the cysteine adduct that drives the actuator's conformational change, leading to dequenching. (nature.com)
Production2
The viral download Ethos Of Pluralization for IL23 is of Interleukin-12 electron production mutation( IL12RB1), which plays reviewed with the Humanitarian inactivation, and Interleukin-23 fraction( IL23R). (evakoch.com)
The best quality of MoS2 nanosheets was achieved using shear-assisted liquid-phase exfoliation as a production method and sodium cholate (SC) as a surfactant. (bvsalud.org)
Cells1
This simultaneously offers on-line monitoring of light-dependent switching and precise quantification of activation-relaxation properties in intact living cells. (nature.com)
Increase1
This catalyzes the gp41 increase of signaling s costs( R-NH2, completed to latter & complex) and Patients( R-NH-NH2, assisted to R-NH-NH-COCH3). (evakoch.com)
Tissue1
And the inadequate tissue penetration depth of external light results in low efficiency of PDT. (bvsalud.org)
Development1
These design strategies may assist in the development of polyphenol-based drugs. (inrae.fr)
Laser2
With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe. (ctsicn.org)
A., Ph.D. Ocular Morphogenesis 136 Coulombre, Jane L., B.S. Determinants of Collagenous Structure in the Avian Corneal Stroma 141 Section on Experimental Pathology Kuwabara, Toichiro, M.D. Anatomical and Pathological Studies of Ocular Tissues 143 Effect of Laser on the Retina 149 Light Effect on the Retina 151 iix Laboratory of Vision Research (cont. (nih.gov)
Images2
Method of making images on a sensitized surface by exposure to light or other radiant energy. (umassmed.edu)
It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. (ctsicn.org)