MEKCElectrospray ionization mZone electrophoresisConducted by capillary electrophoresisSeparationChromatographic methodCyclodextrin-modifiedDeterminationMass spectrometryUncoated fused silicaDetectionElectrophoresis systemPreconcentrationElectrolyteLiquidBufferCompoundsElectrophoreticDetectorSampleHighGeneralSectionHumanSpecificPositive
MEKC7
- Very often, CE refers to capillary zone electrophoresis (CZE), but other electrophoretic techniques including capillary gel electrophoresis (CGE), capillary isoelectric focusing (CIEF), capillary isotachophoresis and micellar electrokinetic chromatography (MEKC) belong also to this class of methods. (wikipedia.org)
- The analysis of testosterone and related steroids by non-specific micellar electrokinetic chromatography (MEKC) was investigated in this study. (vtt.fi)
- In Part 2 of this review article the authors discuss the fundamental principles and practical aspects of electromigration techniques, including capillary zone electrophoresis (CZE), micellar electrokinetic chromatography (MEKC), and capillary electrochromatography (CEC). (chromatographyonline.com)
- A fast and accurate micellar electrokinetic capillary chromatography (MEKC) method was developed for monitoring N-methyl-2-pyrrolidone (NMP) exposure. (infona.pl)
- Micellar electrokinetic chromatography MEKC is used to separate all voltaren gel impurities and degradant analysis. (hotpod.net.au)
- diuretic Micellar electrokinetic chromatography MEKC is used vibramycin in production scale LC. (petit-poivre.fr)
- Micellar electrokinetic chromatography MEKC is galactorrhea used to optimise separation efficiency throughout the run. (drapikowski.pl)
Electrospray ionization m1
- Simultaneous detection and separation of 18 phenethylamine and tryptamine standards by liquid chromatography/electrospray ionization mass spectrometry, Analytical Sciences, vol. 25, Jul. (ntnu.edu.tw)
Zone electrophoresis1
- Title: Stability-indicating capillary zone electrophoresis method for the assessment of recombinant human granulocyte-macrophage colony-stimulating factor and its correlation with reversed-phase liquid chromatography method and bioassay. (aablocks.com)
Conducted by capillary electrophoresis1
- Analyses of the extracts were conducted by capillary electrophoresis in micellar electrokinetic chromatography modality. (atpasepathway.com)
Separation14
- Capillary electrophoresis (CE) is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro- and nanofluidic channels. (wikipedia.org)
- Separation by capillary electrophoresis can be detected by several detection devices. (wikipedia.org)
- Owing to their electrical neutrality, however, separation of steroids by capillary electromigration techniques requires the use of charged electrolyte additives that interact with the steroids either specifically or non-specifically. (vtt.fi)
- The development of two-dimensional systems, performed by coupling either liquid chromatography (LC) with an electromigration technique or two electromigration techniques, operated under different separation mechanisms, is also discussed. (chromatographyonline.com)
- Column-based liquid phase separation techniques, such as liquid chromatography (LC) in reversed phase separation mode and capillary electromigration techniques, using continuous electrolyte systems, are widely used for the identification and quantification of phenolic compounds in plants and food matrices of plant origin. (chromatographyonline.com)
- Baseline separation of NMP and its main metabolites: 5-hydroxy-N-methyl-2-pyrrolidone (5HNMP), N-methylsuccinimide (MSI), 2-hydroxy-N-methylsuccinimide (2HMSI), and 2-pyrrolidone (2P) was obtained within 6 min in an uncoated fused silica capillary using 5 mM phosphate buffer and 140 mM sodium dodecyl sulfate (pH 7.1) as background electrolyte (BGE). (infona.pl)
- Z. Zhang, B. Yan, K. Liu, Y. Liao, H. Liu*，Separation and fragmentation study of heroin and its basic impurities by reversed- phase liquid chromatography coupled to tandem mass spectrometry, Rapid Commun. (siaoyin.com)
- Title: Separation of fluorescently labeled phosphoinositides and sphingolipids by capillary electrophoresis. (aablocks.com)
- Title: Separation and determination of chiral composition in penicillamine tablets by capillary electrophoresis in a broad pH range. (aablocks.com)
- Optimization of the separation and on-line sample concentration of N,N-dimethyltryptamine and related compounds using micellar electrokinetic chromatography, J. Sep. (ntnu.edu.tw)
- With the use of capillaries as separation columns and the construction of automated capillary electrophoresis devices, IEF has also been reformed. (kapillarelektrophorese.com)
- After separation of the sample, the entire capillary content must be "mobilized" to enable detection. (kapillarelektrophorese.com)
- After completion of the focusing process (recognizable by the almost constant current), a pressure is applied parallel to the separation voltage and the entire capillary content is thus guided past the detector. (kapillarelektrophorese.com)
- The disadvantage is that the EOF already flushes small amounts of the capillary content out of the capillary at the beginning of the separation and thus a loss of sample and ampholyte can occur. (kapillarelektrophorese.com)
Chromatographic method1
- A micellar electrokinetic capillary chromatographic method for determining anionic catecholamine metabolites from patient urine samples was established. (vtt.fi)
Cyclodextrin-modified1
- Title: Determination of hydroxy acids in cosmetics by chemometric experimental design and cyclodextrin-modified capillary electrophoresis. (aablocks.com)
Determination7
- Multivariate optimization of large-volume sample stacking with polarity switching by capillary electrophoresis for determination of gold nanoparticle size. (ccu.edu.tw)
- 6. Ying-Xuan Huang, Yu-Ying Chao, Yi-Hui Lin, Jing-Ru Liou, Hai-Chi Chan, Yen-Ling Chen* Determination of 13-cis-Retinoic Acid and Its Metabolites in Plasma by Micellar Electrokinetic Chromatography Using Cyclodextrin-Assisted Sweeping for Sample Preconcentration, Molecules , 2021, 26(19), 5865. (ccu.edu.tw)
- 8. Hung-Yu Ko, Yi-Hui Lin, Chi-Jen Shih, Yen-Ling Chen*, Determination of phenylenediamines in hair colors derivatizated with 5-(4, 6-dichlorotriazinyl) aminofluorescein via micellar electrokinetic chromatography, Journal of Food and Drug Analysis, 2019, 27,825-831. (ccu.edu.tw)
- 10. Chun-Hsien Chen, Yu-Ying Chao, Yi-Hui Lin, Yen-Ling Chen*, Determination of finasteride and its metabolite in urine by dispersive liquid-liquid microextraction combined with field-enhanced sample stacking and sweeping, Journal of chromatography A, 2018, 1547, 14-20. (ccu.edu.tw)
- 11. Feng-Lin Yen, Shih-Wei Wang, Hui-Ling Cheng, Kuan-Ling Chen, Yen-Ling Chen*, Determination of Saikosaponins in Bupleuri Radix by micellar electrokinetic chromatography with experimental design. (ccu.edu.tw)
- Title: Determination of imatinib mesylate and related compounds by field amplified sample stacking with large volume sample injection capillary electrophoresis. (aablocks.com)
- Title: Capillary electrophoresis with field-amplified sample stacking for rapid and sensitive determination of sulfadiazine and sulfamethoxazole. (aablocks.com)
Mass spectrometry3
- Capillary electrophoresis was first combined with mass spectrometry by Richard D. Smith and coworkers, and provides extremely high sensitivity for the analysis of very small sample sizes. (wikipedia.org)
- Applications of Hadamard transform-gas chromatography/mass spectrometry to online detection of exhaled breath after drinking or smoking, Journal of Chromatography A, vol. 1217, Jul. (ntnu.edu.tw)
- Application of Hadamard transform to gas chromatography/nonresonant multiphoton ionization/time-of-flight mass spectrometry, J. Sep. (ntnu.edu.tw)
Uncoated fused silica1
- When uncoated fused silica capillaries are used, the entire capillary content is moved through the EOF towards the cathode and thus past the detector. (kapillarelektrophorese.com)
Detection6
- Certain aspects of the instrumentation (such as detection) are necessarily more complex than for a single-capillary system, but the fundamental principles of design and operation are similar to those shown in Figure 1. (wikipedia.org)
- In these systems, a section of the capillary itself is used as the detection cell. (wikipedia.org)
- The portion of the capillary used for UV detection, however, must be optically transparent. (wikipedia.org)
- The path length of the detection cell in capillary electrophoresis (~ 50 micrometers) is far less than that of a traditional UV cell (~ 1 cm). (wikipedia.org)
- Development and Application of a Milli-Whistle for Use in Gas Chromatography Detection, Anal. (ntnu.edu.tw)
- The disadvantage is that analytes with a very high pI may be focused within the capillary path of detection and cathode. (kapillarelektrophorese.com)
Electrophoresis system1
- A basic schematic of a capillary electrophoresis system is shown in figure 1. (wikipedia.org)
Preconcentration1
- This paper contributes to the field of the on-line preconcentration of enantiomers by developing a novel setup based on the electrokinetic accumulation of ketoprofen enantiomers on the pH boundary followed by enantioselective mobilization by a mixture of SDS, sulfated-β-cyclodextrin (S-β-CD), and trimethyl-β-cyclodextrin (TM-β-CD). (edu.pl)
Electrolyte1
- The source vial, destination vial and capillary are filled with an electrolyte such as an aqueous buffer solution. (wikipedia.org)
Liquid6
- Despite the very small sample sizes (typically only a few nanoliters of liquid are introduced into the capillary), high sensitivity and sharp peaks are achieved in part due to injection strategies that result in a concentration of analytes into a narrow zone near the inlet of the capillary. (wikipedia.org)
- Part 1 will focus on liquid chromatography. (chromatographyonline.com)
- Chiral separative methods are also seledruff shampoo considerable developments in liquid chromatography. (hotpod.net.au)
- Chromatography, Liquid" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (childrensmercy.org)
- This graph shows the total number of publications written about "Chromatography, Liquid" by people in this website by year, and whether "Chromatography, Liquid" was a major or minor topic of these publications. (childrensmercy.org)
- Below are the most recent publications written about "Chromatography, Liquid" by people in Profiles. (childrensmercy.org)
Buffer2
- This is achieved in either pressure or electrokinetic injections simply by suspending the sample in a buffer of lower conductivity (e.g. lower salt concentration) than the running buffer. (wikipedia.org)
- The pH gradient is created by first filling the entire capillary with an ampholyte solution, which also contains the sample solution, a stabilizing medium, buffer substances and possibly urea. (kapillarelektrophorese.com)
Compounds1
- This paper is the first of a two-part review article discussing fundamental and practical aspects of both LC and capillary electromigration techniques used for the analysis of phenolic compounds occurring in plant-derived food and in edible and medicinal plants. (chromatographyonline.com)
Electrophoretic1
- The analytes separate as they migrate due to their electrophoretic mobility, and are detected near the outlet end of the capillary. (wikipedia.org)
Detector2
- The system's main components are a sample vial, source and destination vials, a capillary, electrodes, a high voltage power supply, a detector, and a data output and handling device. (wikipedia.org)
- These are then flushed out of the capillary without having passed the detector. (kapillarelektrophorese.com)
Sample3
- To introduce the sample, the capillary inlet is placed into a vial containing the sample. (wikipedia.org)
- Sample is introduced into the capillary via capillary action, pressure, siphoning, or electrokinetically, and the capillary is then returned to the source vial. (wikipedia.org)
- To achieve greater sample throughput, instruments with arrays of capillaries are used to analyze many samples simultaneously. (wikipedia.org)
High3
- Such capillary array electrophoresis (CAE) instruments with 16 or 96 capillaries are used for medium- to high-throughput capillary DNA sequencing, and the inlet ends of the capillaries are arrayed spatially to accept samples directly from SBS-standard footprint 96-well plates. (wikipedia.org)
- With the advent of chemically bonded fused capillary columns which offered high efficiencies and thermal theophylline microscopy. (petit-poivre.fr)
- A low pH value (e.g. phosphoric acid) is then introduced to the anode side of the capillary (positive pole), while a solution with a high pH value (e.g. diluted NaOH) is introduced to the cathode side (negative pole). (kapillarelektrophorese.com)
General1
- In general, capillaries used in capillary electrophoresis are coated with a polymer (frequently polyimide or Teflon) for increased flexibility. (wikipedia.org)
Section2
- This bare section of capillary can break easily, and capillaries with transparent coatings are available to increase the stability of the cell window. (wikipedia.org)
- Alternatively, the critical capillary section could be filled with the cathode solution (diluted NaOH) to locally advance the pH gradient. (kapillarelektrophorese.com)
Human1
- Finally, new data for interactions between steroids and human and bovine serum albumins were obtained through the use of affinity capillary electrophoresis. (vtt.fi)
Specific1
- Focusing to a specific point in the capillary is automatic: if an analyte leaves its own zone (e.g. by diffusion), it enters a range with a different pH value and regains an electrical charge that causes it to migrate back into its own zone (which corresponds to the pI). (kapillarelektrophorese.com)
Positive1
- In the most common mode of CE, all ions, positive or negative, are pulled through the capillary in the same direction by electroosmotic flow. (wikipedia.org)