• gels
  • Agarose exhibits the phenomenon of thermal hysteresis in its liquid-to-gel transition, i.e. it gels and melts at different temperatures. (wikipedia.org)
  • On standing the agarose gels are prone to syneresis (extrusion of water through the gel surface), but the process is slow enough to not interfere with the use of the gel. (wikipedia.org)
  • Commercial gels like PLgel, Sephadex, Bio-Gel (cross-linked polyacrylamide), agarose gel and Styragel are often used based on different separation requirements. (wikipedia.org)
  • N. Volpi, F. Maccari: Detection of submicrogram quantities of glycosaminoglycans on agarose gels by sequential staining with toluidine blue and Stains-All. (wikipedia.org)
  • bead
  • As this isn't a "dumb" elution by time (as in size exclusion chromatography) but an elution with Imidazole in which I can determine the exact amount to add to specifically elute my protein of interest empirically, so the resolution should be the same no matter the bead size. (protocol-online.org)
  • To prevent steric interference or overlap during the binding process of the target molecule to the ligand, an inhibitor containing a hydrocarbon chain is first attached to the agarose bead (solid support). (wikipedia.org)
  • This inhibitor with a hydrocarbon chain is commonly known as the spacer between the agarose bead and the target molecule. (wikipedia.org)
  • columns
  • Purolite Life Sciences today announced its collaboration with Repligen Corporation (NASDAQ:RGEN), a life sciences company focused on bioprocessing technology leadership and the maker of the market-leading OPUS® line of pre-packed chromatography columns. (environmental-expert.com)
  • reactions
  • Zero EEO agaroses are also available but these may be undesirable for some applications as they may be made by adding positively charged groups that can affect subsequent enzyme reactions. (wikipedia.org)
  • liquid
  • It may be a liquid (LC and Capillary Electrochromatography (CEC)), a gas (GC), or a supercritical fluid (supercritical-fluid chromatography, SFC). (wikipedia.org)
  • form
  • Cyanogen bromide is also often used because it reacts with the hydroxyl groups on agarose to form cyanate esters and imidocarbonates. (wikipedia.org)
  • When solidified, the fibres form a three-dimensional mesh of channels of diameter ranging from 50 nm to >200 nm depending on the concentration of agarose used - higher concentrations yield lower average pore diameters. (wikipedia.org)
  • different
  • A variety of chemically modified agaroses with different melting and gelling temperatures are available through chemical modifications. (wikipedia.org)
  • high
  • It was in the fifties and sixties that theoretical models were developed for IC for further understanding and it was not until the seventies that continuous detectors were utilized, paving the path for the development from low-pressure to high-performance chromatography. (wikipedia.org)
  • polar
  • In the case of HPLC the mobile phase consists of a non-polar solvent(s) such as hexane in normal phase or a polar solvent such as methanol in reverse phase chromatography and the sample being separated. (wikipedia.org)
  • commonly
  • The melting and gelling temperatures of agarose can be modified by chemical modifications, most commonly by hydroxyethylation, which reduces the number of intrastrand hydrogen bonds, resulting in lower melting and setting temperatures than standard agaroses. (wikipedia.org)