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Chromatography, High Pressure LiquidChromatography, AffinitySepharoseChromatography, GelChromatographyChromatography, Ion ExchangeChromatography, GasElectrophoresis, Agar GelChromatography, LiquidChromatography, Thin LayerChromatography, AgaroseMolecular WeightElectrophoresis, Polyacrylamide GelChromatography, DEAE-CelluloseMolecular Sequence DataKineticsMass SpectrometryAmino Acid SequenceGas Chromatography-Mass SpectrometryAmino AcidsChromatography, Reverse-PhaseChromatography, PaperIsoelectric FocusingCattleHydrogen-Ion ConcentrationChemistryChemical PhenomenaSubstrate SpecificityEscherichia coliImmunodiffusionMethodsElectrophoresisSpectrophotometry, UltravioletBase SequenceCarbohydratesImmunoelectrophoresisTandem Mass SpectrometryIsoelectric PointDNARabbitsChemical FractionationPolysaccharidesMacromolecular SubstancesProtein BindingSpectrometry, Mass, Electrospray IonizationDrug StabilityIndicators and ReagentsTemperatureLiverCarbohydrate SequencePeptide FragmentsOligosaccharidesGelsRecombinant ProteinsCountercurrent DistributionTrypsinChromatography, Micellar Electrokinetic CapillarySolubilityHydrolysisMagnetic Resonance SpectroscopyCentrifugation, Density GradientCloning, MolecularGlycoproteinsChemical PrecipitationCell LinePeptidesPlasmidsUltracentrifugationGlycoside HydrolasesHydroxyapatitesReference StandardsCells, CulturedIsoenzymesSpecies SpecificityReproducibility of ResultsPolymerase Chain ReactionBinding SitesSwineMicrochemistrySensitivity and SpecificityDNA, BacterialRadioimmunoassayGlycosaminoglycansImmunoelectrophoresis, Two-DimensionalCulture MediaLectinsCarbohydrate ConformationDNA Restriction EnzymesTime FactorsBacterial ProteinsHot TemperatureBiological AssayNucleic Acid HybridizationMolecular StructureCalibrationMicroscopy, ElectronBlood ProteinsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationCarrier ProteinsRats, Inbred Strains
Purified by affinity chromatographyCovalentlyBeadsHydrophobic Interaction ChSize exclusion chroPhenyl Agarose 6 Fast FlowLigandAffinity Chromatography MediaProteinsBeadResinsResinScale chromatographyPermeation chromatographyElectrophoresisFiltrationPurificationAnalyticalMatrixPeptidePolysaccharidePolyacrylamideGelsMoleculesOctylSepharoseBufferCross-linkedPROTEINDEXLiquidAminoApplicationsBovineInhibitionProductsChains
Purified by affinity chromatography2
Covalently2
Beads6
  • G-Sep™ Agaroses for HIC are also available with butyl and octyl groups immobilized on 6% cross-linked agarose beads through neutral and highly stable ether linkages. (gbiosciences.com)
  • The chromatography column is packed with fine, porous beads which are commonly composed of dextran, agarose, or polyacrylamide polymers. (wikipedia.org)
  • The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads. (abcam.com)
  • The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. (jacksonimmuno.com)
  • SMART Chromatography™ NTA is packed with large cross-linked agarose beads, derivatized with metal-free nitrilotriacetic acid (NTA) ligand. (empbiotech.com)
  • 400 µg protein yield) underwent phosphopeptide enrichment using magnetic Fe 3+ NTA agarose beads before being TMT labeled and fractionated by capillary liquid chromatography (LC). (nih.gov)
Hydrophobic Interaction Ch4
  • G-Sep™ Phenyl Agarose Fast Flow (FF) is a 6% cross-linked agarose with phenyl groups for Hydrophobic Interaction Chromatography (HIC). (gbiosciences.com)
  • It is suitable for use in Hydrophobic Interaction Chromatography (HIC) to s. (gbiosciences.com)
  • Hydrophobic interaction chromatography (HIC) separates molecules based on their hydrophobicity. (marvelgent.com)
  • PROTE INDEX ™ Hydrophobic Interaction Chromatography media Butyl Agarose 4 Fast Flow, Octyl Agarose 4 Fast Flow, Phenyl Agarose 6 Fast Flow (Low Phenyl) and Phenyl Agarose 6 Fast Flow (High Phenyl) are optimized for high throughput, high-resolution, high-capacity separation of proteins and other biomolecules based on their hydrophobicity. (marvelgent.com)
Size exclusion chro7
Phenyl Agarose 6 Fast Flow1
Ligand5
  • G-Sep™ Phenyl Agarose Fast Flow is available in two levels of ligand substitution degree to help to find the optimal selectivity and binding capacity for a given application. (gbiosciences.com)
  • Affinity chromatography is the preferred method of bioselective adsorption and subsequent recovery of a compound from an immobilized ligand. (sigmaaldrich.com)
  • The product is made of specially engineered alkali-resistant Protein A ligand cross-linked to a novel agarose gel matrix. (genscript.com)
  • The spacer arm is attached to the agarose before coupling of the ligand. (elastin.com)
  • The agarose derivatives will retain approximately 90% of ligand potency during a 12 month period when stored as indicated above. (elastin.com)
Affinity Chromatography Media2
  • Alkaline-tolerant Protein A Affinity Chromatography Media (Monofinity A Resin, Cat. (genscript.com)
  • Protein G SepFast is a family of affinity chromatography media for the purification of immunoglobulins and binds to the Fc region of IgG from a variety of mammalian species. (biokal.com)
Proteins7
  • PROTE INDEX ™ Ion Exchange Media CM, SP, DEAE, Q Agarose 6FF are specifically developed for high-resolution separation of proteins, peptides, nucleic acids and other charged biomolecules according to their difference in surface charge. (marvelgent.com)
  • Ni-NTA Agarose is an affinity chromatography matrix for purifying recombinant proteins carrying a His tag. (qiagen.com)
  • Ni-NTA Agarose provides Ni-NTA coupled to a Sepharose CL-6B support and offers high binding capacity and minimal nonspecific binding (see figure One-step purification under native conditions. One-Step Purification under Native Conditions. Human serum response factor (SRF) was expressed in HeLa cells carrying a vaccinia virus vector and purified using Ni-NTA Agarose with different imidazole concentrations in the wash and elution steps. Proteins were visualized by silver staining. CL : cell lysate; FT : flow-through; W1 : 0.8 mM wash; W2 & W3 : 8 mM wash; W4 & W5 : 40 mM wash; E1 & E2 : 80 mM elution. (Data kindly provided by H. Stunnenberg, EMBL, Heidelberg, Germany.) "> One-step purification under native conditions ). (qiagen.com)
  • In addition to sterilizing and inactivating viruses, it can also dissolve denatured proteins, lipids, and DNA molecules deposited on the top of the column, to extend the service life chromatography media. (genscript.com)
  • The stability of surface-exposed proteins was determined by measuring the recovery of biotin-labeled proteins with affinity chromatography. (molvis.org)
  • Avantor has the resources to make your Chromatography or Mass Spectrometry applications run efficiently and effectively-from the measuring apparatus needed for chromatography, or the proteins used to fulfill sample manipulation during mass spectrometry. (vwr.com)
  • Separation of nucleic acids and proteins by electrophoresis, chromatography or immunodiffusion. (hispanagar.com)
Bead1
  • 6% BCL Agarose Bead Standard is a crosslinked agarose resin with a bead size of 50-150 µm. (abtbeads.com)
Resins5
  • Chromatography helps in refinement of biomolecules and is considered to be convincing resins for purification of protein applications. (visiongain.com)
  • The chromatography resins market is estimated to grow significantly with an increase of application in food industry. (visiongain.com)
  • In pharmaceutical domain the chromatography resins are used for unscrambling chiral compounds that are biologically active and immense potent drugs. (visiongain.com)
  • Chromatography resins thus enables to gain highly recovered and pure pharmaceutical intermediate. (visiongain.com)
  • Furthermore, increasing emphasis of drug manufacturers to search for cure for viruses such as Ebola and Zika is thereby accentuating the demand for chromatography resins that is further contributing towards significant growth in global chromatography resin market. (visiongain.com)
Resin13
  • The global chromatography resin market was valued at $1.9 billion in 2018. (visiongain.com)
  • Extensive adoption of chromatography as a splitting technique in food & beverage industry is the key factor driving the growth of global chromatography resin market. (visiongain.com)
  • In food industry chromatography resin is used for viewing the nutritional quality of food along with vitamin C content. (visiongain.com)
  • The detection of vitamin C content in the final products which is an essential function of chromatography resin as it helps in depicting the decreasing value of nutritional content in the food that is further tagged as unsuitable for human consumption. (visiongain.com)
  • These detection values are used by manufacturers in various food products such as drinks, vegetables, fresh and frozen fruits, juices and powered drinks thereby significantly contributing towards rapid growth of the global chromatography resin market. (visiongain.com)
  • Prospecting growth in pharmaceutical industry globally is another factor that is pertaining towards the growth of global chromatography resin market. (visiongain.com)
  • However, stringent regulatory reforms imposed on the manufacturing process and existence of other alternative techniques in the market such as protein crystallization, ultra-filtration membrane and electrophoresis are the key factors hampering the growth of global chromatography resin market. (visiongain.com)
  • Chromatography resin market forecast 2020 - 2030 estimates the historical market size in terms of consumption as well as revenue generated by the industry players across the globe. (visiongain.com)
  • It offers the opportunity analysis along the value chain for the chromatography resin manufacturers. (visiongain.com)
  • On the basis of technique, the global chromatography resin market is segmented into affinity, size exclusion, ion exchange, hydrophobic interaction, and others. (visiongain.com)
  • Amongst these segments, affinity chromatography technique is anticipated to grow at the fastest rate during the forecast period owing to increasing demand for binding agents from the manufacturers of Asia-Pacific that helps in satisfying their needs and require iminodiacetic acid (IDA) and protein A/G further supporting global chromatography resin market to grow at significant rate. (visiongain.com)
  • Protein A affinity chromatography resin is the most commonly applied tool for the isolation and purification of IgG. (genscript.com)
  • SMART Chromatography™ FPLC column packed with NTA Agarose Resin. (empbiotech.com)
Scale chromatography2
  • Marvelgent ion exchanger media are optimized for high sample loading capacity with reliable, reproducible performance, suited for process scale chromatography. (marvelgent.com)
  • Protein G SepFast is developed and supported for production scale chromatography. (biokal.com)
Permeation chromatography2
  • Typically, when an aqueous solution is used to transport the sample through the column, the technique is known as gel-filtration chromatography, versus the name gel permeation chromatography, which is used when an organic solvent is used as a mobile phase. (wikipedia.org)
  • however, it was not until 1964, when J. C. Moore of the Dow Chemical Company published his work on the preparation of gel permeation chromatography (GPC) columns based on cross-linked polystyrene with controlled pore size, that a rapid increase of research activity in this field began. (wikipedia.org)
Electrophoresis3
Filtration4
Purification4
  • Purification of bovine pineal hydroxyindole O-methyltransferase by immunoadsorption chromatography. (nih.gov)
  • 6. Rapid purification of porcine colostral whey lactoferrin by affinity chromatography on single-stranded DNA-agarose. (nih.gov)
  • 15. Purification of a 76-kDa iron-binding protein from human seminal plasma by affinity chromatography specific for ribonuclease: structural and functional identity with milk lactoferrin. (nih.gov)
  • It is specifically designed for SMART Chromatography™ IMAC protein purification. (empbiotech.com)
Analytical1
  • 1. Analytical cation-exchange chromatography to assess the identity, purity, and N-terminal integrity of human lactoferrin. (nih.gov)
Matrix4
  • The base matrix of PROTE INDEX ™ IEX-CM, IEX-SP, IEX-DEAE, IEX-Q is highly cross-linked 6% agarose, providing excellent chemical and physical stability. (marvelgent.com)
  • The anionic groups of the agarose gel are attached to the matrix and cannot migrate, while the dissociable countercations migrate to the cathode within the matrix to generate EEO. (sigmaaldrich.com)
  • Also, ligands are attached to the hydrocarbon spacer arms ethylenediamine succinic acid and 3,3'-diaminodipropylamine succinic acid which extend the ligands approximately 13 A and 21 A, respectively, from the agarose matrix. (elastin.com)
  • The base matrix of Marvelgent ProteIndex™ is highly cross-linked agarose, providing high efficiency and super physical and chemical stability for both lab scale and processing scale applications. (marvelgent.com)
Peptide1
  • Peptide affinity chromatography demonstrated that soybean lipoxygenase V specifically bound to synthetic peptides E5b and P1 immobilized to agarose. (nih.gov)
Polysaccharide1
  • Agarose is a polysaccharide that is isolated and purified from agar or agar-bearing marine algae (sea kelp). (sigmaaldrich.com)
Polyacrylamide1
  • other gels with size fractionation properties include agarose and polyacrylamide. (wikipedia.org)
Gels1
  • pHast Pack™ ready-to-pour agarose gels are available in 1% agarose in TAE buffer or 1% agarose in TBE buffer . (sigmaaldrich.com)
Molecules1
Octyl1
  • Octyl Agarose 4FF medium is optimized for high-resolution, high-capacity separation of biomolecules. (marvelgent.com)
Sepharose1
  • Ligands of the agarose derivatives described below are attached to CNBr-activated beaded agarose (Sepharose* 4B). (elastin.com)
Buffer1
  • Unless otherwise indicated, all agarose derivatives are supplied as packed gel in the storage buffer 0.02 M NaOAc pH 5 containing 0.01% NaN3 as preservative. (elastin.com)
Cross-linked1
  • These are based on large-particle highly cross-linked agarose, which is surface-modified to provide the desired binding functionality. (empbiotech.com)
PROTEINDEX1
Liquid1
  • Agarose solutions exhibit hysteresis when transitioning from liquid to gel. (sigmaaldrich.com)
Amino1
Applications2
  • The Ni-NTA Agarose is intended for molecular biology applications. (qiagen.com)
  • We provide a wide array of agarose products with varying gel strengths, melting temperatures, gelling temperatures, and electroendosmosis (EEO) levels to accommodate all of your research applications. (sigmaaldrich.com)
Bovine1
  • A procedure for the isolation of osteopontin (OPN) from bovine milk using ion-exchange and hydrophobic chromatography is described. (tamu.edu)
Inhibition1
  • The capacity for production of migration inhibitory factor was assessed by the agarose droplet cell migration inhibition assay, using peritoneal exudate cells and a CVB3(m) cell lysate or KCl extracted antigens from heart tissues of CVB3(m)-inoculated mice. (nih.gov)
Products2
  • In the biopharmaceutical industry, in order to ensure high quality of products, it is often necessary to carry out clean-in-place (CIP) of chromatography systems. (genscript.com)
  • The products you use, the products you need, the suppliers you trust for chromatography. (vwr.com)
Chains1
  • The linear agarose polymer forms chains which create fibers that are flexible and form a web of channels with diameters from 50 nm to 200 nm, depending on the amount of agarose used. (sigmaaldrich.com)