• AChE
  • Notably, agonist-dependent stimulation of P2Y 2 R elevated the neuronal expression of cholinergic genes encoding AChE, PRiMA (an anchor for the globular form AChE) and choline acetyltransferase, and this induction was mediated by a signaling cascade that involved Ca 2+ mobilization and ERK1/2 activation. (aspetjournals.org)
  • metabolism
  • NMDA receptor-induced excitotoxicity has been hypothesized to mediate abnormal choline (Cho) metabolism that is involved in alterations in membrane permeability and cell death in certain neurodegenerative disorders. (jneurosci.org)
  • To determine whether NMDA receptor overactivation modulates choline metabolism in vivo , we investigated the effects of NMDA on interstitial choline concentrations using microdialysis. (jneurosci.org)
  • These results show that NMDA modulates choline metabolism, eliciting a receptor-mediated, calcium-dependent, and region-specific increase in extracellular choline from membrane phospholipids that is not mediated by phospholipase A 2 and precedes delayed excitotoxic neuronal cell death. (jneurosci.org)
  • rats
  • medical citation needed] Coluracetam has been shown to reverse the loss of choline acetyltransferase production in the medial septal nucleus of rats exposed to phencyclidine (PCP), and is considered a potential therapeutic drug for schizophrenia. (wikipedia.org)
  • concentration
  • The NMDA-evoked increase in dialysate choline was calcium and concentration dependent and was prevented with 1 m m AP-5, a competitive NMDA antagonist, but was not altered by mepacrine, a phospholipase A 2 inhibitor. (jneurosci.org)
  • ChAT
  • Choline is bound in the active site of ChAT by non-covalent interactions between the positively charged amine of choline and the hydroxyl group of Tyr552, in addition to a hydrogen bond between choline's hydroxyl group and a histidine residue, His324. (wikipedia.org)
  • The choline substrate fits into a pocket in the interior of ChAT, while acetyl-CoA fits into a pocket on the surface of the protein. (wikipedia.org)
  • These amacrine cells are usually characterised by their expression of choline acetyltransferase, or ChAT and are known to play a role in direction selectivity and detection of directional motion. (wikipedia.org)