• Cav-1-containing lipid rafts and redistribution of Cav-1, PKCgamma, and Cx43 were analyzed by sucrose gradients and by consequent Western blot analysis. (nih.gov)
  • TPA and IGF-1 induced redistribution of Cav-1 and Cx43 from light-density fractions to higher density fractions on sucrose gradients. (nih.gov)
  • Isopycnic density-gradient ultracentrifugation is a density-based separation technique whereby a medium, such as sucrose, is added in layers of increasing density from top to bottom to cover the range of densities found in the sample. (pall.com)
  • The sperm are placed in the solution and then spun in a centrifuge, causing them to separate based on their density. (androwash.in)
  • Diamond Max™ Centrifuge Tubes are engineered for high-speed centrifugation and meet the demands of the most critical scientific work. (thomassci.com)
  • The sample is applied in a thin zone at the top of the centrifuge tube on a density gradient. (toppr.com)
  • Centrifugation with a centrifuge that develops centrifugal fields of more than 100,000 times gravity. (bvsalud.org)
  • PBMCs were isolated from leukapheresis products of healthy volunteers by density gradient centrifugation. (nih.gov)
  • At certain centrifugal forces, there is a high probability of extracting/pelleting other particles with the same buoyant density, size, and mass as the target exosomes. (pall.com)
  • UHPSFC , ultra- pacted by other parameters, such as the addition of the mod- high performance supercritical fluid chromatography ifier and the change of the mobile phase density [20]. (lu.se)
  • Centrifugation is a mechanical process which involves the use of the centrifugal force to separate particles from a solution according to their size, shape, density, medium viscosity and rotor speed. (wikipedia.org)
  • The larger the size and the larger the density of the particles, the faster they separate from the mixture. (wikipedia.org)
  • The particles' settling velocity in centrifugation is a function of their size and shape, centrifugal acceleration, the volume fraction of solids present, the density difference between the particle and the liquid, and the viscosity. (wikipedia.org)
  • This problem can be overcome with the use of moving-zone density-gradient centrifugation, a method that separates particles by size and density. (pall.com)
  • After centrifugation, the particles will be divided into different heights in the medium gradient 1 . (pall.com)
  • In this way, particles with the same density can be separated based on size. (pall.com)
  • The Big Easy" EasySep™ Magnet generates a high-gradient magnetic field in the interior cavity that is strong enough to separate cells labeled with EasySep™ Magnetic Particles without the use of columns. (stemcell.com)
  • 2) The principle of the centrifugation technique is to separate the particles suspended in liquid media under the influence of a centrifugal field. (toppr.com)
  • When two particles having different sizes but same densities are suspended in any liquid medium then they may not be able to separate by a simple filtration method. (toppr.com)
  • Under centrifugal force, the particles will begin segmenting through the gradient. (toppr.com)
  • The particles will begin segmenting in separate zones according to their size, shape and density. (toppr.com)
  • There is a correlation between the size and density of a particle and the rate that the particle separates from a heterogeneous mixture, when the only force applied is that of gravity. (wikipedia.org)
  • Centrifugation is a process which separates or concentrates materials suspended in a liquid medium. (toppr.com)
  • When this container is rotated at high-speed mixture separated into its constituent parts by the action of centrifugal force on their density. (toppr.com)
  • With differential centrifugation, a cell culture sample is separated based on density, size, and shape. (pall.com)
  • RÉSUMÉ La présente étude a examiné les cellules souches hématopoïétiques de 19 cas de syndrome myélodysplasique de haut risque à la recherche de signaux apoptotiques et anti-apoptotiques et de proliférations cellulaires et a établi un lien entre ces derniers et les sous-types cytogénétiques et cliniques, en particulier la trisomie 8. (who.int)
  • The sample is then added to the top of the gradient medium, and typically centrifuged at a very high speed over a long period of time. (pall.com)
  • The moving-zone gradient ultracentrifugation method has two gradient medium layers - a low density top layer, lower than all the solutes in the sample, and a very high-density bottom layer which acts as a cushion to protect against potential exosome pelleting. (pall.com)
  • To avoid this, more gentle techniques such as double density gradient centrifugation and swim-up procedures have been developed and are now widely used in clinical practice. (androwash.in)
  • a rough guide to the 'g' force exerted at this speed is to multiply the centrifugation radius by a factor of 10, so a radius of 160 mm gives approximately 1600 x g. (wikipedia.org)
  • SDF, spectral density function. (lu.se)
  • The density differences between extracellular components can be exploited to separate exosomes from contaminants. (pall.com)
  • Under healthy physiological conditions, activities of osteoclasts and osteoblasts are tightly controlled by both systemic and local factors, maintaining bone mineral density and bone microarchitecture. (hindawi.com)
  • With differential centrifugation, a cell culture sample is separated based on density, size, and shape. (pall.com)
  • In contrast to a differential centrifugation method from the literature, analysis of cell-compartment marker proteins shows that the Qproteome Mitochondria Isolation Kit provides mitochondrial fractions virtually free of cross-contamination (see figure "Mitochondrial fractions virtually free of cross-contamination" ). (qiagen.com)
  • Concentrate the virus by three cycles of differential centrifugation. (dpvweb.net)
  • The exosomes were purified by differential centrifugation and ultracentrifugation, followed by density gradient fractionation. (nih.gov)
  • Lung leukocytes were obtained over the course of infection by enzyme digestion of lung tissue followed by centrifugation over Percoll discontinuous density gradients. (cdc.gov)
  • Intact cells are separated from cell membranes by centrifugation through a single step discontinuous density gradient and the pellet finally resuspended in medium appropriate for cell culture or flow cytometric analysis. (worthington-biochem.com)
  • Density Gradient Centrifugation (also known as Ficoll-Paque 1 , Pancoll 2 , or Lymphoprep 3 ) is the most common method for separating PBMC (perioheral blood mononuclear cells - containing lymphocytes and monocytes) from red blood cells (RBC, erythrocytes). (pluriselect.com)
  • PBMC were isolated by density gradient centrifugation (Ficoll-Paque™ PLUS, Cat. (bdbiosciences.com)
  • Different Density Gradient Media for the isolation of Granuolcyte, PBMC, Monocyte, PLT and more. (pluriselect.com)
  • Peripheral blood mononuclear cells (PBMC) are isolated from whole blood samples by Ficoll gradient density centrifugation. (amc.nl)
  • The density differences between extracellular components can be exploited to separate exosomes from contaminants. (pall.com)
  • The exosomes and contaminants, including protein aggregates, apoptotic bodies, microvesicles, and viruses, will settle in a static position in the correlating layer of the same density 1 . (pall.com)
  • Cesium-chloride gradient centrifugation -- A laboratory technique used to separate or purify nucleic acids in a solution. (nih.gov)
  • Beside of the most common solution for the isolation of PBMCs (density 1.077 g/ml) pluriSelect offers optimized density gradient media for the isolation of Leukocytes, Monocytes, Platelets and PBMCs from older blood. (pluriselect.com)
  • Here we report a detailed protocol for serotype independent production of AAV using a helper-free HEK293 cell system followed by iodixanol gradient purification, a method described earlier. (nih.gov)
  • Purification and concentration of influenza inactivated viruses by continuous-flow zonal centrifugation. (nih.gov)
  • A simplification of the density-gradient procedure for the purification of influenza virus. (nih.gov)
  • Furthermore pluriMate® and TwinSpin® centrifugation tubes simplify handling and accelerate purification. (pluriselect.com)
  • At equilibrium each particle settles in the gradient at a point equal to its density. (nih.gov)
  • Density gradient centrifugation enables scientists to separate substances based on size, shape, and density. (asu.edu)
  • Two-step centrifugation method. (nih.gov)
  • Centrifugation is also the most common method used for uranium enrichment, relying on the slight mass difference between atoms of U-238 and U-235 in uranium hexafluoride gas. (wikipedia.org)
  • Based on different density of each blood cell population they can be separated by a single step centrifugation with the corresponding density gradient medium. (pluriselect.com)
  • Here in this particular technique, a force is applied to a solution that helps it to separate itself from the dense components from those with relatively low density. (vedantu.com)
  • Centrifugation is used to separate a mixture that has two specific miscible liquids in it and both the liquids are different from each other. (vedantu.com)
  • Centrifugation is applied to separate the fat from the skimmed milk and leaving the skimmed milk alone in its fat form. (vedantu.com)
  • Plasma and/or serum is isolated from whole blood by centrifugation and stored at -80°C in Sanyo MDF series upright freezers. (amc.nl)
  • PBMCs were isolated from leukapheresis products of healthy volunteers by density gradient centrifugation. (nih.gov)