• 2017
  • SMi Group proudly presents the inaugural launch of 3D Cell Culture, an exciting new conference scheduled to take place on the 22nd and 23rd February 2017, London UK. (smi-online.co.uk)
  • 3D Cell Culture 2017 will bring together leading professionals and researchers in the industry to discuss the latest developments and future potential of this technique. (smi-online.co.uk)
  • 3D Cell Culture 2017 will present success stories 3D culture applications, identifying the newest and brightest technologies, and the translation of 3D models into successful clinical and industrial applications. (smi-online.co.uk)
  • Corning Incorporated will showcase its life sciences solutions Nov. 9 and 10 at the 2017 Cell Culture & Bioprocessing Congress in London, U.K. (corning.com)
  • vitro cell
  • Eric Simon, in a 1988 NIH SBIR grant report, showed that electrospinning could be used to produced nano- and submicron-scale polystyrene and polycarbonate fibrous mats (now known as scaffolds) specifically intended for use as in vitro cell substrates. (wikipedia.org)
  • toxicity
  • This often compromises experiments in basic life science, leads to misleading drug-screening results on efficacy and toxicity, and produces cells that may lack the characteristics needed for developing tissue regeneration therapies. (wikipedia.org)
  • Organotypic liver cell cultures can be used e.g. in drug discovery for testing liver toxicity and metabolism of the novel drug candidates. (wikipedia.org)
  • protein
  • This group has been working on showing this goes on in human stem cells for at least 2 years, who knows how long it took them to find this out in mice, or narrow down this one specific protein. (slashdot.org)
  • Several different types of hydrogels from natural and synthetic materials are available for 3D cell culture, including e.g. animal ECM extract hydrogels, protein hydrogels, peptide hydrogels, polymer hydrogels, and wood-based nanocellulose hydrogel. (wikipedia.org)
  • There are currently more than 100 insect cell lines available for recombinant protein production with lines derived from Bombyx mori, Mamestra brassicae, Spodoptera frugiperda, Trichoplusia ni, and Drosophila melanogaster being of particular interest. (wikipedia.org)
  • Insects cell lines are used because post translational modifications of protein are possible in insect cells whereas this mechanism is not present in prokaryotic system The Sf9 is one of the most commonly used cell lines in insect cell culture. (wikipedia.org)
  • Many schemes for 3D culturing are being developed or marketed, such as bio-reactors or protein-based gel environments. (wikipedia.org)
  • SILAC (stable isotope labeling by/with amino acids in cell culture) is a technique based on mass spectrometry that detects differences in protein abundance among samples using non-radioactive isotopic labeling. (wikipedia.org)
  • SILAC has emerged as a very powerful method to study cell signaling, post translation modifications such as phosphorylation, protein-protein interaction and regulation of gene expression. (wikipedia.org)
  • Developed by Protein Sciences Corporation, FluBlok is produced with insect cells. (wikipedia.org)
  • Methods
  • Iwould think there would be plenty of references in is Journal of Tissue CulTure Methods. (bio.net)
  • The 3D cell culture market is predicted to reach $3702.2 million by 2021* with main increase seen in novel technologies and culture methods. (smi-online.co.uk)
  • In addition, there will be focus on organoid technology and the involvement of 3D culture methods in drug and toxicology screening, topics of great current commercial interest to pharmaceutical and research bodies. (smi-online.co.uk)
  • There will be emphasis on the success and future of 3D methods in organoid research, disease modeling and toxicology screening, stem cell research, and much more. (smi-online.co.uk)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. (wikipedia.org)
  • In general, the platforms can be classified in two types of 3D culturing methods: 1) scaffold techniques and 2) scaffold-free techniques. (wikipedia.org)
  • In general, there are two types of 3D culture methods: scaffold techniques and scaffold-free techniques. (wikipedia.org)
  • Several tissue culture methods which measure the rate of chemical absorption by the skin have been approved by the OECD. (wikipedia.org)
  • Another approach has been the development of test methods that use cultured human cells. (wikipedia.org)
  • human
  • When last I interacted with them 2 years ago, their labs were doing primary keratinocyte cultures from human newborn foreskins on a weekly basis. (bio.net)
  • Science Daily reports that for the first time, human embryonic stem cells have been cultured under chemically controlled conditions without the use of animal substances, which is essential for future clinical uses. (slashdot.org)
  • Now, for the first time, we can produce large quantities of human embryonic stem cells in an environment that is completely chemically defined," says professor Karl Tryggvason, who led the study at Sweden's Karolinska Institutet. (slashdot.org)
  • This early use of electrospun fibrous lattices for cell culture and tissue engineering showed that various cell types including Human Foreskin Fibroblasts (HFF), transformed Human Carcinoma (HEp-2), and Mink Lung Epithelium (MLE) would adhere to and proliferate upon the fibers. (wikipedia.org)
  • There are similar possibilities for AIDS research: the RCCS can produce human HIV host cells that can be infected and studied. (wikipedia.org)
  • EpiDerm from Mattek and EpiSkin and SkinEthic RHE model are derived from human skin cells which have been cultured to produce a model of human skin. (wikipedia.org)
  • Human epidermal keratinocytes have been cultured to mimic the human epidermis, and are used to measure skin irritation and dermal corrosion. (wikipedia.org)
  • viral
  • Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)
  • The potential use of cell culture techniques in developing viral vaccines, especially for the Influenza virus, has been widely investigated in recent years as a complementary and alternative platform to the current egg-based strategies. (wikipedia.org)
  • 22nd
  • Building on the sell-out success of the launch event, SMi Group is delighted to announce the return of 3D Cell Culture , taking place on 21st and 22nd of February 2018, in London UK. (smi-online.co.uk)
  • artificial
  • An established or immortalized cell line has acquired the ability to proliferate indefinitely either through random mutation or deliberate modification, such as artificial expression of the telomerase gene. (wikipedia.org)
  • This initiates cell-cell interactions in the absence of any artificial surface or matrix. (wikipedia.org)
  • Artificial cells, blood substitutes, and immobilization biotechnology. (wikipedia.org)
  • insights
  • Our 3-day event, formed of two half day post-conference workshops and a two-day conference will provide useful insights into the latest developments within cell culture. (smi-online.co.uk)
  • proteins
  • There have been some breakthroughs on 1, last I heard a group had shown you can just culture with modified proteins to induce pluripotency. (slashdot.org)
  • When the cells are growing in this medium, they incorporate the heavy arginine into all of their proteins. (wikipedia.org)
  • The trick is that the proteins from both cell populations can be combined and analyzed together by mass spectrometry. (wikipedia.org)
  • refers
  • Microfluidics refers to a set of technologies for the manipulation for the small fluid volumes within artificially fabricated microsystems.And cell culture refers to the maintenance and growth of cells in a controlled laboratory environment. (wikipedia.org)
  • This refers to the in vitro assessment of material to determine whether it releases toxic chemicals in sufficient quantities to kill cells either directly or indirectly through the inhibition of cell metabolic pathways. (wikipedia.org)
  • rapidly
  • Whilst still in its infancy, 3D Cell Culture is rapidly growing with not only a widespread reach that can be seen across a number of different fields such as 3D bioprinting and microfluidics, but also with incredible potential for industrial application. (smi-online.co.uk)
  • A 3D cell culturing system known as the Bio-Assembler™ uses biocompatible polymer-based reagents to deliver magnetic nanoparticles to individual cells so that an applied magnetic driver can levitate cells off the bottom of the cell culture dish and rapidly bring cells together near the air-liquid interface. (wikipedia.org)
  • growth
  • Professor Przyborski is developing new and innovative ways to manage the growth and function of cultured cells. (smi-online.co.uk)
  • however, only the white cells are capable of growth in culture. (wikipedia.org)
  • Alternatively, pieces of tissue can be placed in growth media, and the cells that grow out are available for culture. (wikipedia.org)
  • Aside from temperature and gas mixture, the most commonly varied factor in culture systems is the cell growth medium. (wikipedia.org)
  • The third dimension of cell growth provides more contact space for mechanical inputs and for cell adhesion, which is necessary for integrin ligation, cell contraction and even intracellular signalling. (wikipedia.org)
  • In 2D, the cells must undergo regular trypsinization in order to provide them with sufficient nutrients for normal cell growth. (wikipedia.org)
  • 3D spheroids have been cultured in a lab setting for up to 302 days while still maintaining healthy, non-cancerous growth. (wikipedia.org)
  • Oxygen, required by cells for growth, is fed into the liquid medium through a porous wall in the chamber. (wikipedia.org)
  • One of the cell populations is fed with growth medium containing normal amino acids. (wikipedia.org)
  • viability
  • For the majority of isolated primary cells, they undergo the process of senescence and stop dividing after a certain number of population doublings while generally retaining their viability (described as the Hayflick limit). (wikipedia.org)
  • Appropriate dilutions are then made, depending on cell number in the starting population, as well as the viability and characteristics of the cells being cloned. (wikipedia.org)
  • The 3T3 Neutral Red Uptake (NRU) Phototoxicity Test, approved by the OECD, detects the viability of 3T3 cells after exposure to a chemical in the presence or absence of light. (wikipedia.org)
  • embryonic
  • IPsC are when they take cells from your own body and make them revert back to a similar state to embryonic stem cells, to where they can then be turned into any cell type you want (the advantage there being they're your cells so you wouldn't get tissue rejection like you would with embryonic stem cells. (slashdot.org)
  • In 1885, Wilhelm Roux removed a portion of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the principle of tissue culture. (wikipedia.org)
  • lines
  • Does anybody know the origins of the Schneider cell and Kc cell lines? (bio.net)
  • cell lines (my lit searches have turned up nothing). (bio.net)
  • On page 118, he gives in-text citation of the original papers describing the origins of the cell lines, on pages 133-138 he gives the complete bibliographical references. (bio.net)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • Numerous cell lines are well established as representative of particular cell types. (wikipedia.org)
  • The European Collection of Authenticated Cell Cultures houses and supplies cell lines. (wikipedia.org)
  • August 1, 1920 - October 4, 1951) was an African American woman whose cancer cells are the source of the HeLa cell line, the first immortalized cell line and one of the most important cell lines in medical research. (wikipedia.org)
  • Even though some information about the origins of HeLa's immortalized cell lines was known to researchers after 1970, the Lacks family was not made aware of the line's existence until 1975. (wikipedia.org)
  • line
  • To maximize cell yield, choose from our comprehensive line of standard and custom culture media, including basal salt solutions, antibiotics, sera, and specialty media. (corning.com)
  • These isolated B cells are in turn fused with immortal B cell cancer cells, a myeloma, to produce a hybrid cell line called a hybridoma, which has both the antibody-producing ability of the B-cell and the exaggerated longevity and reproductivity of the myeloma. (wikipedia.org)
  • The myeloma cell line that is used in this process is selected for its ability to grow in tissue culture and for an absence of antibody synthesis. (wikipedia.org)
  • Although originally derived from a mouse embryo, the 3T3 cell line was developed in 1962. (wikipedia.org)
  • These cells were then cultured by George Otto Gey who created the cell line known as HeLa, which is still used for medical research. (wikipedia.org)
  • microgravity
  • The Rotary Cell Culture System (RCCS) is a device designed to grow three-dimensional cell clusters in microgravity. (wikipedia.org)
  • In the early 1990s, NASA researchers began developing hardware that would let them study the cell tissues of mammals-including humans-in microgravity. (wikipedia.org)
  • The chamber rotates around a horizontal axis, allowing the cells to develop in an environment similar to the free-fall of microgravity. (wikipedia.org)
  • environment
  • Create an ideal environment for growing cells, right from the start. (corning.com)
  • A 3D cell culture is an artificially created environment in which biological cells are permitted to grow or interact with their surroundings in all three dimensions. (wikipedia.org)
  • For example the direct contact method offers conditions which are most similar to the physiological environment but the cells are susceptible to trauma if the material moves. (wikipedia.org)
  • laboratory
  • Early studies in the 80's, led by Mina Bissell from the Lawrence Berkeley National Laboratory, highlighted the importance of 3D techniques for creating accurate in vitro culturing models. (wikipedia.org)
  • Biotechnology
  • One complication of these blood-derived ingredients is the potential for contamination of the culture with viruses or prions, particularly in medical biotechnology applications. (wikipedia.org)
  • vaccine
  • The injectable polio vaccine developed by Jonas Salk was one of the first products mass-produced using cell culture techniques. (wikipedia.org)
  • This vaccine was made possible by the cell culture research of John Franklin Enders, Thomas Huckle Weller, and Frederick Chapman Robbins, who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • Verorab, developed by Sanofi Pasteur, is a mammalian vero cell based rabies vaccine approved by the World Health Organization. (wikipedia.org)
  • technology
  • Professor Przyborski holds an academic position as Professor in Cell Technology at Durham University. (smi-online.co.uk)
  • Understanding the interplay between critical cell culture parameters and the micro environmental conditions created by microfluidic devices will accelerate the microfluid cell culture technology. (wikipedia.org)
  • Using RCCS technology, researchers could potentially test chemotherapy agents on a patient's own cancer cells from outside the patient's body. (wikipedia.org)