• protein
  • The protein, called LHPP, prevents the uncontrolled proliferation of cancer cells in the liver. (innovations-report.com)
  • or you can do the vitality assay on cells with different numbers than normalize your assay with total protein or nucleic acid contents. (protocol-online.org)
  • Because I used MTT for cell viability, I can not use the exact same well for protein assay. (protocol-online.org)
  • In summary, we uncovered a signalling pathway operating specifically in T-ALL cells in which ORP4L mediates G protein-coupled ligand-induced PLCβ3 activation, resulting in an increase of mitochondrial respiration for cell survival. (nature.com)
  • Radiation mainly works by producing free radicals that reacts with biomolecules (DNA, protein and lipid) in mammalian and other types of cells. (rockland-inc.com)
  • This study adds to our previously published work because it implicates the TNF family member protein called BAFF in the 'revved up' B-cell signaling we found in our patients. (eurekalert.org)
  • I also investigated the repression of the transcription factor c-Jun, by itself and by Krox-20, in immature Schwann cells and found that this occurs mainly at the protein, rather than the transcriptional level. (bl.uk)
  • Survival and PCD mechanisms are mediated through adaptor protein binding to the death domain of the p75NTR cytoplasmic tail. (wikipedia.org)
  • This protein domain is highly important to cell survival. (wikipedia.org)
  • Studies in Xenopus oocytes suggest that this protein alone and in combination with pannexin 2 may form cell type-specific gap junctions with distinct properties. (wikipedia.org)
  • The DENV E (envelope) protein, found as a dimer on the surface of the mature viral particle, is important in the initial attachment of this particle to the host cell. (wikipedia.org)
  • endothelial cells
  • 6-10 Evidence suggests that EPCs contribute as much as 25% of endothelial cells (ECs) in newly formed blood vessels, 11 and transplantation of EPCs into patients has been demonstrated to induce blood flow recovery in ischemic limbs 12 and increase myocardial viability after infarction. (ahajournals.org)
  • receptors
  • However, NGF binds at least two receptors on the surface of cells that are capable of responding to this growth factor, TrkA (pronounced "Track A") and the LNGFR. (wikipedia.org)
  • There is evidence that pancreatic beta cells express both the TrkA and p75NTR receptors of NGF. (wikipedia.org)
  • Cells which express both the LNGFR and the Trk receptors might therefore have a greater activity - since they have a higher "microconcentration" of the neurotrophin. (wikipedia.org)
  • cellular
  • We demonstrate that ORP4L is expressed in T-ALL but not normal T-cells and its abundance is proportional to cellular ATP. (nature.com)
  • The rise in free cytosolic intracellular Ca 2+ concentration results in the activation of Ca 2+ -dependent phospholipases and proteases that further hasten the rate of membrane depolarisation, leading to uncontrolled cellular swelling and, ultimately, to cell necrosis ( Hochachka, 1986 ) ( Fig.1 ). (biologists.org)
  • Lkb1 -deficient bone marrow cells exhibit mitochondrial defects, alterations in lipid and nucleotide metabolism, and depletion of cellular ATP. (pubmedcentralcanada.ca)
  • TrkA is a receptor tyrosine kinase (meaning it mediates its actions by causing the addition of phosphate molecules on certain tyrosines in the cell, activating cellular signaling). (wikipedia.org)
  • This is useful for control of cellular signaling because the di-phosphorylated phosphoinositide PIP2 is only phosphorylated by the family of enzymes, PI 3-kinases (phosphoinositide 3-kinase or PI3-K), and only upon receipt of chemical messengers which tell the cell to begin the growth process. (wikipedia.org)
  • nerve
  • Immature Schwann cells mature into the myelinating and non-myelinaling Schwann cells present in the adult nerve. (bl.uk)
  • I found that STAT3 is activated by, and supports survival following stimulation by autocrine factors secreted by Schwann cells both in vitro and following nerve injury in vivo. (bl.uk)
  • They are also nerve cell survival factors. (wikipedia.org)
  • The low-affinity nerve growth factor receptor (nerve growth factor receptor (TNFR superfamily, member 16), also called the LNGFR or p75 neurotrophin receptor) is one of the two receptor types for the neurotrophins, a family of protein growth factors that stimulate neuronal cells to survive and differentiate. (wikipedia.org)
  • Two distinct subpopulations of Olfactory ensheathing glia (being researched for nerve repair) have been identified with high or low cell surface expression of low-affinity nerve growth factor receptor (p75). (wikipedia.org)
  • regulate
  • Lkb1 can also regulate energy homeostasis and cell structure through its capacity to phosphorylate and activate 12 other AMPK-related kinases 11 . (pubmedcentralcanada.ca)
  • AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. (wikipedia.org)
  • Metabolic
  • Jessica Allen, PhD, the paper's first author, says "We found that B cells from patients with active chronic GVHD were in a heightened metabolic state and resist programmed cell death. (eurekalert.org)
  • The single most protective and unifying feature of cold-tolerance in hibernating and neonatal mammals is the same one that ensures their enhanced survival in O 2 -limiting conditions: a regulated metabolic depression. (biologists.org)
  • In contrast, an early mismatch between ATP supply and demand in 'anoxia- and cold-sensitive cells' leads to a forced hypometabolism which is, in effect, early metabolic failure. (biologists.org)
  • Instead, these data define a central role for Lkb1 in restricting HSC entry into cell cycle and in broadly maintaining energy homeostasis in haematopoietic cells through a novel metabolic checkpoint. (pubmedcentralcanada.ca)
  • The metabolic control systems in HSCs are poorly understood although these cells have been shown to be highly sensitive to energetic and oxidative stress and must be able to shift between quiescence and highly proliferative states to maintain the haematopoietic system under varying physiological conditions 1 - 5 . (pubmedcentralcanada.ca)
  • Given the importance ofHSCdynamics to the haematopoietic system and the sensitivity of haematopoietic cells to metabolic stress, we sought to investigate the function of Lkb1 in haematopoiesis. (pubmedcentralcanada.ca)
  • neuron
  • NGF is released from the target cells, binds to and activates its high-affinity receptor tropomyosin receptor kinase A (TrkA), and is internalized into the responsive neuron. (wikipedia.org)
  • This mechanism may be a distinct way to control the survival of a neuron. (wikipedia.org)
  • inhibitor
  • And what is the inhibitor for EDTA after cells are detached? (protocol-online.org)
  • 66, 6800-6806 Sun, Y., Hegamyer, G. and Colburn, N. H. (1994) Molecular cloning of five messenger RNAs differentially expressed in preneoplastic or neoplastic JB6 mouse epidermal cells: one is homologous to human tissue inhibitor of metalloproteinases-3. (wikipedia.org)
  • TrkA
  • The NGF/TrkA complex is subsequently trafficked back to the cell body. (wikipedia.org)
  • Study results indicate that superior cervical ganglia neurons that express both p75NTR and TrkA die when treated with proNGF, while NGF treatment of these same neurons results in survival and axonal growth. (wikipedia.org)
  • induces
  • In the process of inflammation, NGF is released in high concentrations by mast cells, and induces axonal outgrowth in nearby nociceptive neurons. (wikipedia.org)
  • The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. (wikipedia.org)
  • Lineage
  • In this study I investigated the effects of BMP in the Schwann cell lineage in vitro. (bl.uk)
  • Lkb1 mRNA expression is readily detectable in haematopoietic cells in the bone marrow, with highest levels in HSCs 12 and in multipotent and lineage-restricted progenitors compared to committed Lin + cells ( Supplementary Fig. 1a ). (pubmedcentralcanada.ca)
  • Responses
  • These cell types are well characterised and can be readily distinguished from one another by their distinct antigenic profile, survival responses, and morphological changes. (bl.uk)
  • I also found that survival responses to BMP2/4 differ between embryonic and postnatal Schwann cell. (bl.uk)
  • This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. (wikipedia.org)
  • neural crest
  • The generation of mature Schwann cells from the neural crest occurs by a transition through two intermediate cell types, namely the Schwann cell precursor and the immature Schwann cell. (bl.uk)
  • Gene knockdown of CD271 has also been shown to abolish neural crest stem cell properties of melanoma cells. (wikipedia.org)
  • chronic
  • This review highlights the key elements in this adaptive response to protection against acute and chronic cell injury provoked by environmental stresses. (mendeley.com)
  • Chapel Hill, NC - Leukemia and lymphoma patients who receive life-saving stem cell or bone marrow transplants often experience chronic side effects that significantly decrease quality of life, can last a lifetime, and ultimately affect their long-term survival. (eurekalert.org)
  • In chronic Graft vs. Host Disease (GVHD), the differences between the donor bone marrow cells and the recipient's body often cause these immune cells to recognize the recipient's body tissues as foreign and the newly transplanted cells attack the transplant recipient's body. (eurekalert.org)
  • For patients who received bone marrow or stem cells, it is estimated that 40-70 percent may experience chronic GVHD. (eurekalert.org)
  • In a paper published in the journal, Blood , a team from the University of North Carolina's Lineberger Comprehensive Cancer Center, shows in the laboratory that B cells from patients with chronic GVHD are much more active than cells from patients without the disease. (eurekalert.org)
  • Dose fractionation Dose-response relationship Chronic radiation syndrome "Cell Survival Curves" (PDF). (wikipedia.org)
  • STAT3
  • At the end of lactation, the loss of the suckling triggers a STAT3-dependent apoptotic program to remove the differentiated alveolar cells. (nih.gov)
  • I examined the role of STAT3 in Schwann cells both in vitro and in vivo using mice with a conditional mutation of STAT3 specifically in Schwann cells. (bl.uk)
  • immune
  • It also was found to reduce the amount of aging in yeast, flies, worms, and human immune cells by inducing autophagy. (wikipedia.org)
  • growth
  • Cells were cultured in endothelial cell basal medium-2 in the absence and presence of CRP (5 to 20 μg/mL), rosiglitazone (1 μmol/L), and/or vascular endothelial growth factor. (ahajournals.org)
  • Haematopoietic stem cells (HSCs) can convert between growth states that have marked differences in bioenergetic needs. (pubmedcentralcanada.ca)
  • The Lkb1 tumour suppressor encodes an evolutionarily conserved serine/threonine kinase that links metabolism and cell growth 6 . (pubmedcentralcanada.ca)
  • genes
  • However, upon recognition of chemical signals imparted by oxidative and electrophilic molecules, Nrf2 is released from Keap1, escapes proteasomal degradation, translocates to the nucleus, and transactivates the expression of several dozen cytoprotective genes that enhance cell survival. (mendeley.com)
  • Genes to Cells. (wikipedia.org)
  • concentrations
  • Whereas the time course of the latter is metered in minutes to hours, the ability to depress ATP turnover in a regulated fashion effectively delays the onset of cell death by stabilising concentrations of adenylates at new hypometabolic steady states for periods of hours or days ( Fig.1 ). (biologists.org)
  • donor
  • The technique involves injection of compatible donor red blood cells of a different blood group into a recipient, followed by blood testing periodically afterwards. (wikipedia.org)
  • Differential agglutination of the red cells is then used to determine the number of remaining donor cells, allowing the survival rate to be determined. (wikipedia.org)
  • mechanism
  • Whereas single lethal events lead to an exponent that is linearly related to dose, the survival fraction function for a two-stage mechanism carries an exponent proportional to the square of dose. (wikipedia.org)
  • acute
  • Here, we report that T-cell acute lymphoblastic leukemia (T-ALL) cells are characterized by increased oxidative phosphorylation and robust ATP production. (nature.com)
  • T-cell acute lymphoblastic leukemia (T-ALL) is one of the deadliest and most aggressive hematological malignancies. (nature.com)
  • expression
  • Melanoma cells transplanted into an immunodeficient mouse model were shown to require expression of CD271 in order to grow a melanoma. (wikipedia.org)
  • Spermidine has also been found to "upregulate expression of the epithelial stem cell-associated keratins K15 and K19, and dose-dependently modulated K15 promoter activity in situ and the colony forming efficiency, proliferation and K15 expression of isolated human K15-GFP+ cells in vitro. (wikipedia.org)
  • survive
  • It prevents the cell communicating the stress that it is under, which is crucial if the cell is to survive. (wikipedia.org)
  • Truncating mutations in this gene have been shown to promote breast cancer metastasis to the lungs by allowing cancer cells to survive mechanical stretch in the microcirculation. (wikipedia.org)
  • type
  • Yes, I neutralize trypsin by serum containing medium and this procedure works well with wield type cells. (protocol-online.org)
  • In the macrophage, the primary signal for activation is IFN-γ from Th1 type CD4 T cells. (wikipedia.org)
  • Trials and meta-analyses have confirmed that the drug is effective on patients with this cancer type, extending their survival. (wikipedia.org)
  • The number of remaining nonagglutinated Type O cells as a function of time defines the survival rate of blood cells. (wikipedia.org)
  • bacteria
  • A team of biologists at the Stanford University School of Medicine employed a new method to estimate the minimum genetic information required for the survival of bacteria called Caulobacter crescentus , which are commonly used in labs. (icr.org)
  • populations
  • ORP4 knockout mice exhibit teratozoospermia due to death of developing spermatozoa, indicating that ORP4 is essential for the survival of specific cell populations 19 . (nature.com)
  • radiation
  • It depicts the relationship between the fraction of cells retaining their reproductive integrity and the absorbed dose of radiation. (wikipedia.org)