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  • Flow
  • In this dissertation a non-adherent immortal cell line was studied under static media flow conditions inside a CO[subscript]2 incubator and under dynamic media flow conditions in a novel portable cell culture chamber. (k-state.edu)
  • In addition to cell trapping, a novel electrokinetic injection method was developed for injecting analytes from a hydrodynamic flow into a separation channel that was followed by an electrokinetic separation. (k-state.edu)
  • As the hydrodynamic flow could introduce some excess band broadening in the separation, the actual band broadening of an analyte was measured for different channel depths and hydrodynamic fluid flow rates. (k-state.edu)
  • analysis
  • There are several potential advantages to the use of microfluidic devices for the analysis of single cells: First, cells can be handled with care and precision while being transported in the microfluidic channels. (k-state.edu)
  • Third, cell culturing and analysis in microfluidic devices uses only extremely small volumes of culturing media and analysis buffer. (k-state.edu)
  • Viability
  • In summary: Minimum effect on the cells High recovery viability Separated cells can be used further Rapid As mentioned above, the CCE separates cells based on their sedimentation property but not specific features (e.g. surface protein, cell shape). (wikipedia.org)
  • For example, Leucosporidium frigidum grows at −2 to 20 °C (28 to 68 °F), Saccharomyces telluris at 5 to 35 °C (41 to 95 °F), and Candida slooffi at 28 to 45 °C (82 to 113 °F). The cells can survive freezing under certain conditions, with viability decreasing over time. (wikipedia.org)
  • FACS
  • Fluorescent Activated Cell Sorting, or FACS, utilizes Flow cytometry to provide a fast, objective and quantitative measurement of intra- and extracellular properties, not including morphology, for sorting a heterogeneous mixture of cells. (wikipedia.org)
  • Fluorescence-activated cell sorting (FACS) is a technique for sorting out the cells based on the differences that can be detected by light scatter (e.g. cell size) or fluorescence emission (by penetrated DNA, RNA, proteins, antigens). (wikipedia.org)
  • suspension
  • The cell suspension is fed to a chamber the walls of which diverge over a certain length thereof and which converge again toward the exit from the chamber. (google.com)
  • Positively selected cells are retained in the tube while negatively selected cells are in the liquid suspension. (wikipedia.org)
  • It is the formation of homogenous mass of cells (cell homogenate or cell suspension). (wikipedia.org)
  • The slurry yeast made by small bakers and grocery shops became cream yeast, a suspension of live yeast cells in growth medium, and then compressed yeast, the fresh cake yeast that became the standard leaven for bread bakers in much of the Westernized world during the early 20th century. (wikipedia.org)
  • CTCs
  • Circulating tumor cells (CTCs) are tumor cells that have detached from primary tumor site and are transported via the circulation system. (hindawi.com)
  • These cells, which shed into vasculature, are referred to as circulating tumor cells (CTCs). (hindawi.com)
  • The importance of CTCs is further augmented when there are increasing evidences about the presence of significance correlation between the number of circulating tumor cells and patients survival times. (hindawi.com)
  • Cell-types often of interest to purify may be specific leukocytes, such as CD4+ T cells, stem cells, or circulating tumor cells (CTCs). (wikipedia.org)
  • Circulating tumor cells (CTCs) are cells that have shed into the vasculature or lymphatics from a primary tumor and are carried around the body in the circulation. (wikipedia.org)
  • The significant efforts put into understanding the CTCs biological properties have demonstrated the critical role circulating tumor cells play in the metastatic spread of carcinoma. (wikipedia.org)
  • Furthermore, highly sensitive, single-cell analysis demonstrated a high level of heterogeneity seen at the single cell level for both protein expression and protein localization and the CTCs reflected both the primary biopsy and the changes seen in the metastatic sites. (wikipedia.org)
  • 2. Cytokeratin negative (CK-) CTCs are cancer stem cells or cells undergoing epithelial-mesenchymal transition (EMT). (wikipedia.org)
  • 3. Apoptotic CTCs are traditional CTCs that are undergoing apoptosis (cell death): Epic Sciences technology identifies nuclear fragmentation or cytoplasmic blebbing associated with apoptosis. (wikipedia.org)
  • Measuring the ratio of traditional CTC to apoptotic CTCs - from baseline to therapy - provides clues to a therapy's efficacy in targeting and killing cancer cells. (wikipedia.org)
  • 4. Small CTCs are cytokeratin positive and CD45 negative, but with sizes and shapes similar to white blood cells. (wikipedia.org)
  • Small CTCs have been implicated in progressive disease and differentiation into small cell carcinomas, which often require a different therapeutic course. (wikipedia.org)
  • tissue
  • Specifically, the neutrophils (a type of white blood cell also known as polymorphonuclear cells) contain a variety of powerful enzymes that can cause tissue inflammation. (google.com)
  • Assist customers in troubleshooting their experiments including but not limited to tissue preparation, magnetic cell separation, flow cytometry analysis, and immune cell based assays. (biotalent.ca)
  • InGeneron develops and manufactures biomedical equipment that is designed to fit into the workflow of an operating physician for the easy recovery of regenerative cells from the adipose tissue. (wikipedia.org)
  • An efficient point-of-care processing system that isolates regenerative cells from adipose tissue has been developed. (wikipedia.org)
  • Consisting of the disposables for processing and the Tissue Processing Unit, the Transpose™ RT System is InGeneron's system that allows for point-of-care preparation of regenerative cells. (wikipedia.org)
  • The proprietary enzyme is designed to quickly and gently release from adipose tissue the adult regenerative cells. (wikipedia.org)
  • It procures, processes and stores human umbilical cord blood and tissue samples that may be used for stem cell treatments. (wikipedia.org)
  • Immunology
  • STEMCELL Technologies is a fast-growing, privately owned biotechnology company looking for a motivated Scientific Sales Representative to help grow our revenue and support the use of our Cell Separation products by customers in the fields of immunology, cancer and cell therapy, among others. (biotalent.ca)
  • Gain an understanding of customers' projects through in-person meetings, phone calls, and emails, in order to position our Cell Separation and Immunology related products as valuable solutions to their needs. (biotalent.ca)
  • The application segment includes stem cell research, immunology research, neuroscience research and cancer research. (medgadget.com)
  • morphology
  • The DEPArray lab-on-a-chip technology platform is designed to individually identify, manipulate and sort specific cells within a heterogeneous population based on intra- and extracellular properties, not including morphology. (wikipedia.org)
  • and are often larger cells with irregular shape or subcellular morphology. (wikipedia.org)
  • STEMCELL Technologies
  • Some of the major companies operating in the global cell separation technologies market are Terumo BCT, Inc., STEMCELL Technologies, Inc., pluriSelect GmbH, Miltenyi Biotec GmbH, Merck Millipore, Life Technologies Corporation and BD Biosciences. (medgadget.com)
  • antibody
  • 5. The device of claim 1 wherein the reagent that specifically binds red blood cell surface antigens is selected from the group consisting of monoclonal antibody, polyclonal antibodies, and their F(ab') 2 , Fab' and Fab fragments. (google.com)
  • Dynabeads may be covalently linked to an antibody that recognizes a specific protein on the surface of the target cell-type. (wikipedia.org)
  • Alternatively, Dynabeads may attach to the cell indirectly, either via streptavidin on the Dynabead linking to a biotinylated primary antibody, or a secondary antibody on the Dynabead linking to the primary antibody. (wikipedia.org)
  • Streptavidin linkage to the primary antibody allows Dynabeads to capture cells with lower expression of the surface protein. (wikipedia.org)
  • regenerative
  • InGeneron develops, manufactures, licenses and sells regenerative cell separation and diagnostics tools. (wikipedia.org)
  • InGeneron's technologies enable the preparation of adipose-derived regenerative cells (ADRC) that contain progenitor and stem cells in humans and animals for autologous use. (wikipedia.org)
  • This system is used in conjunction with the Matrase™ enzyme that ensures a high yield of regenerative cells. (wikipedia.org)
  • yeast
  • S. cerevisiae is currently the only yeast cell known to have Berkeley bodies present, which are involved in particular secretory pathways. (wikipedia.org)
  • In nature, yeast cells are found primarily on ripe fruits such as grapes (before maturation, grapes are almost free of yeasts). (wikipedia.org)
  • can harbor yeast cells from autumn to spring and transmit them to their progeny. (wikipedia.org)
  • The optimum temperature for growth of S. cerevisiae is 30-35 °C (86-95 °F). Two forms of yeast cells can survive and grow: haploid and diploid. (wikipedia.org)
  • The diploid cells (the preferential 'form' of yeast) similarly undergo a simple lifecycle of mitosis and growth. (wikipedia.org)
  • flow
  • 3 . The cell separation device of claim 1 , wherein the flow path is placed within an electric field. (google.es)
  • Flow cytometry Magnetic-activated cell sorting J. Ugelstad & F.K. Hansen, Rubber Chem. (wikipedia.org)
  • The basic principle of separating the cells inside CCE is the balance between centripetal and the counter flow drag force. (wikipedia.org)
  • Then when the flow rate of the buffer solution increases, the solution tends to push the cells towards the middle of the CCE. (wikipedia.org)
  • Finally the CCE rely on centrifugal force and the counter flow drag force to separate the cells, so the speed of separation is fast. (wikipedia.org)
  • There are two instruments in use based on its principle: a) Flow cytometer b) Fluorescence-activated cell sorter The cells can be separated by blocking metabolic reactions. (wikipedia.org)
  • mitosis
  • The haploid cells undergo a simple lifecycle of mitosis and growth, and under conditions of high stress will, in general, die. (wikipedia.org)
  • involves
  • Generally, cancer metastasis involves the spread of cancer cells, whereby the tumor cells detach from primary tumor site and be transported via the circulation system to a distant organ to form secondary tumors. (hindawi.com)
  • It involves grinding of cells in a suitable medium in the presence of certain enzymes with correct pH, ionic composition, and temperature. (wikipedia.org)
  • The procedure involves passing of a single stream of cells through a laser beam so that the scattered light from the cells can be detected and recorded. (wikipedia.org)
  • plant cell
  • In anaerobic bacteria that degrade plant cell walls, exemplified by Clostridium thermocellum, the dockerin domains of the catalytic polypeptides can bind equally well to any cohesin from the same organism. (wikipedia.org)
  • Polygalacturonan, whose major component is galacturonic acid, is a significant carbohydrate component of the pectin network that comprises plant cell walls. (wikipedia.org)
  • Pectin is one of the three polysaccharides present in the plant cell wall, and it plays a role in maintaining the barrier between the inside and outside environment and gives strength to the plant cell walls. (wikipedia.org)
  • stages
  • In this video segment from The Secret of Life, a fertilized egg cell progresses from its earliest stages through cell differentiation and ultimately the development of an embryo, with simple but identifiable body parts including a spinal cord, a heart, and eyes. (nottingham.ac.uk)
  • Since cell size is correlated with cell cycle stages this method also allows the separation of cells at different stages of the cell cycle. (wikipedia.org)
  • The key concept is that larger cells tend to stay within the flowing buffer solution while smaller cells will be washed away follow the buffer solution (different sedimentation property within the buffer solution), and cells will have different sedimentation properties in different cell cycle stages. (wikipedia.org)
  • Granulocytes
  • Granulocytes and B-cells stick to the fibers, while T-cells and other cells do not. (polysciences.com)
  • After being centrifuged, the following layers will be visible in the conical tube, from top to bottom: plasma and other constituents, a layer of mono-nuclear cells called buffy coat (PBMC/MNC), Ficoll-Paque, and erythrocytes & granulocytes which should be present in pellet form. (wikipedia.org)
  • Note that some red blood cell trapping (presence of erythrocytes & granulocytes) may occur in the PBMC or Ficoll-Paque layer. (wikipedia.org)
  • Cancer
  • Cell separation techniques play a vital role in the treatment of chronic diseases, such as AIDS and cancer. (medgadget.com)
  • For instance, the deficiencies of resolution in imaging modalities have precluded them to image small numbers of cancer cells before angiogenic switch, which in turn limit the detection sensitivity [ 2 , 3 ]. (hindawi.com)
  • Some of the possible clinical applications for this technique include phasing of multiple mutations when parental samples are unavailable, preimplantation genetic diagnosis, prenatal diagnosis and in the characterization of cancer cells. (wikipedia.org)
  • 2016
  • About InGeneron retrieved on April 18, 2016 InGeneron Appoints Angelo Moesslang as Chief Executive Officer retrieved October 20, 2017 InGeneron Announces Changes in Management Team retrieved February 20, 2017 InGeneron Equine Imprint retrieved April 18, 2016 Eticur) Press Release retrieved October 29, 2015 "Why use adipose-derived stem cells? (wikipedia.org)
  • 2016) showed that the intestine of Polistes dominula favors the mating of S. cerevisiae strains, both among themselves and with S. paradoxus cells by providing environmental conditions prompting cell sporulation and spores germination. (wikipedia.org)
  • whereby
  • It is an extracorporeal blood separation method whereby whole blood is extracted from a donor or patient, the red blood cells are separated, and the remaining blood is returned to circulation. (wikipedia.org)
  • specific
  • Code-division multiple access (CDMA) This uses a digital modulation called spread spectrum which spreads the voice data over a very wide channel in pseudorandom fashion using a user or cell specific pseudorandom code. (wikipedia.org)
  • The centrifugal force will separate the red blood cells from other cells due to their high specific weight. (wikipedia.org)
  • scientific
  • In 2015, the German-based stem cell banking company eticur) was acquired with the idea of supporting eticur) with scientific evidence from InGeneron's laboratory. (wikipedia.org)
  • In public banking, parents can donate their baby's cord blood to the public/community bank, to be used to save the life of someone requiring a stem cell transplant or in scientific research. (wikipedia.org)
  • blood
  • 3. A blood filtering device that includes the cell separation device of claim 1 . (google.com)
  • 1968). This rapid separating technique is effective for purifying large quantities of similar cells and has been used to prepare a wide variety of vertebrate cells (whole blood, brain and liver cells, transformed tumor cells). (beckman.com)
  • The sample receiving zone contains an irreversibly immobilized reagent that allows for removal of substantially all red blood cells from the whole blood sample. (google.com)
  • Erythrocytapheresis is an apheresis procedure by which erythrocytes (red blood cells) are separated from whole blood. (wikipedia.org)
  • This is because the hematocrit, or the percentage of blood volume taken up by red blood cells, is present in the highest percentage of all blood cell components in the solid portion of blood. (wikipedia.org)
  • This blood separation is also used in patients with severe malaria. (wikipedia.org)
  • After removal of the affected cells, the patient can then be transfused with a dose of normal red blood cells. (wikipedia.org)
  • This includes either two standard units of red blood cells or one unit plus of red blood cells and another of either plasma or platelets. (wikipedia.org)
  • The advantage to the blood bankers is the on-line separation into standardized RBC masses with the subsequent reduction in testing, data entry and staffing. (wikipedia.org)
  • Capillary and Pressure Driven Microchip for Blood Separation and Assay. (wikipedia.org)
  • Diehl A, Frey G. Optimization of a Blood Separation Process Based on Simulation. (wikipedia.org)
  • phase separation
  • By using the sequential layering he was able to immobilize each layer of nanomaterial and polymer and to overcome the long-standing problem of phase separation known from earlier works on clay nanocomposites carried out by Lagaly and scientists at Toyota. (wikipedia.org)
  • earliest
  • Supposedly, this separation from the sites of earliest antigen processing and capture provide a protected environment in which opsonized antigens can be displayed for a long time without being proteolyzed or removed by phagocytic cells. (wikipedia.org)
  • The earliest known event after his separation from Baelfire is Hook's meeting the exiled fairy Tinker Bell, with whom he shares his reasons for desiring revenge: the Dark One killed the woman he loved. (wikipedia.org)
  • undergo
  • In addition, post-meiotic germ cells undergo a dramatic morphological reshaping process as well as a global epigenetic reconfiguration of the germ line chromatin-the histone-to-protamine switch. (jove.com)
  • In normal lymphoid tissue recirculating resting B cells migrate through the FDC networks, whereas antigen-activated B cells are intercepted and undergo clonal expansion within the FDC networks, generating germinal centers (GC). (wikipedia.org)
  • This results in the formation of GC, where antigen-activated B cells are trapped to undergo somatic mutation, positive and negative selection, isotype switching, and differentiation into high-affinity plasma cells and memory B cells. (wikipedia.org)
  • Activated B-cells with low affinity to antigen captured on FDCs surface as well as autoreactive B-cells undergo apoptosis, whereas B cells bound to FDCs through the antigen complex, survive due to apoptosis blockage caused by interaction with FDCs. (wikipedia.org)
  • magnetic
  • Major physical and chemical methods include magnetic separation, froth flotation, leaching etc., whereby the impurities and unwanted materials are removed from the ore and the base ore of the metal is concentrated, meaning the percentage of metal in the ore is increased. (wikipedia.org)
  • solar
  • Power was supplied by solar cells and rechargeable batteries It decayed from orbit on 9 April 1964. (wikipedia.org)
  • Layered graphene and graphene oxide nanocomposites with a variety of polymers are now used in a variety of charge storage devices: batteries, supercapacitors, fuel cells, solar energy conversion, etc. (wikipedia.org)
  • Kotov received his MS (1987) and PhD (1990) degrees in chemistry from Moscow State University where his research concerned liquid-liquid interfaces imitating cell membranes for solar energy conversion. (wikipedia.org)
  • processes
  • During spermatogenesis in mammals and in Drosophila melanogaster, male germ cells develop in a series of essential developmental processes. (jove.com)
  • The field is a materials science, covering all aspects of the types of ore, washing, concentration, separation, chemical processes and extraction of pure metal and their alloying to suit various applications, sometimes for direct use as a finished product, but more often in a form that requires further working to achieve the given properties to suit the applications. (wikipedia.org)
  • Separation processes take advantage of physical properties of the materials. (wikipedia.org)
  • Electrometallurgy involves metallurgical processes that take place in some form of electrolytic cell. (wikipedia.org)
  • They form a stable network due to intercellular connections between FDCs processes and intimate interaction with follicular B cells. (wikipedia.org)
  • tissues
  • Combining two-photon microscopy with fluorescent tracers, we successfully visualized the engrafted tissues at the single cell level in live mice over several months. (jove.com)
  • Imaging using confocal/two-photon hybrid microscopy enables simultaneous high resolution assessment of uniquely marked cells and their progeny in conjunction with structural components of the tissues. (jove.com)
  • Volumetric analyses over large areas reveal that spectrally coded HSPC-derived cells can be detected non-invasively in various intact tissues, including the bone marrow (BM), for extensive periods of time following transplantation. (jove.com)
  • Factor Mfge produced in lymphoid tissues mainly by FDCs is known to enhance engulfment of apoptotic cells. (wikipedia.org)
  • areas
  • Follicular DCs are a non-migratory population found in primary and secondary follicles of the B cell areas of lymph nodes, spleen, and mucosa-associated lymphoid tissue (MALT). (wikipedia.org)
  • high
  • We developed and validated a fluorescent marking methodology for clonal tracking of hematopoietic stem and progenitor cells (HSPCs) with high spatial and temporal resolution to study in vivo hematopoiesis using the murine bone marrow transplant experimental model. (jove.com)
  • look
  • Variations in angles can create additional separation, which'll make you look more ripped. (hubpages.com)
  • Researchers investigating precise properties of these neurons with respect to disparity present visual stimuli with different disparities to the cells and look whether they are active or not. (wikipedia.org)
  • process
  • Prokaryotic DNA replication is the process by which a prokaryote duplicates its DNA into another copy that is passed on to daughter cells. (wikipedia.org)
  • Fused salt electrolysis is another electrometallurgical process whereby the valuable metal has been dissolved into a molten salt which acts as the electrolyte, and the valuable metal collects on the cathode of the cell. (wikipedia.org)
  • primary
  • Brain cells (neurons) in a part of the brain responsible for processing visual information coming from the retinae (primary visual cortex) can detect the existence of disparity in their input from the eyes. (wikipedia.org)
  • normal
  • Interaction between FDCs precursors and lymphoid cells mediated by TNF-a and lymphotoxin (LT) is crucial for normal FDC development and maintenance. (wikipedia.org)
  • single
  • 5. A. Mahmoodzadeh, H.R. Abutalebi, H. Soltanian-Zadeh , H. Sheikhzadeh, "Single Channel Speech Separation with a Frame-based Pitch Range Estimation Method in Modulation Frequency," Presented at and Published in the Proceedings of the Fifth International Symposium on Telecommunications (IST'2010), Tehran, Iran, Dec. 04-06, 2010. (henryford.com)
  • time
  • Four dimensional (three-dimensional (3D) plus time) information of individual cells was obtained from this imaging. (jove.com)
  • For a time the vagina is represented by a solid rod of epithelial cells. (wikipedia.org)
  • Media conditions that support fast growth in bacteria also couples with shorter inter-initiation time in them, i.e. the doubling time in fast growing cells is less as compared to the slow growth. (wikipedia.org)
  • Unhappy with her management though, Connor teamed up with manager Carlo Vista, who got her signed as Sarah Connor with producer George Glueck's label X-Cell Records a short time after. (wikipedia.org)
  • leads
  • The stimulation of CXCR5 on B cells upregulates LT production, which leads to FDCs activation and stimulates further CXCL13 secretion, thus generating a positive feed-forward loop. (wikipedia.org)
  • The odd number of chromosomes causes the defective cells to die, which leads to the malformations associated with Roberts syndrome. (wikipedia.org)
  • growth
  • In other words, it is possible that in fast growth conditions the grandmother cells starts replicating its DNA for grand daughter cell. (wikipedia.org)
  • dynamic
  • Three-dimensional dynamic deformation of a red blood cell in a dual-trap optical tweezers is computed with the elastic membrane theory and is compared with the experimental results. (osapublishing.org)