• In recent years, interest in the possible molecular regulators of cell proliferation and differentiation in a wide range of regeneration models has grown significantly, but the cell kinetics of this process remain largely a mystery. (mdpi.com)
  • Many of these miRNAs act as tumor suppressors and/or oncogenes, and are involved in cell activities that include development, differentiation, proliferation, apoptosis, metabolism and immunity ( 7 , 10 , 11 ). (spandidos-publications.com)
  • Dnmt3a regulates both proliferation and differentiation of mouse neural stem cells. (ca.gov)
  • DNA methylation is known to regulate cell differentiation and neuronal function in vivo. (ca.gov)
  • Here we examined whether deficiency of a de novo DNA methyltransferase, Dnmt3a, affects in vitro differentiation of mouse embryonic stem cells (mESCs) to neuronal and glial cell lineages. (ca.gov)
  • Early-passage neural stem cells (NSCs) derived from Dnmt3a-deficient ESCs exhibited a moderate phenotype in precocious glial differentiation compared with wild-type counterparts. (ca.gov)
  • Thus, our work revealed a novel role for Dnmt3a in regulating both the timing of neural cell differentiation and the cell proliferation in the paradigm of mESC-derived-NSCs. (ca.gov)
  • Asymmetric inheritance of organelle and cellular compounds between daughter cells impacts on the phenotypic variability and was found to be a hallmark for differentiation and rejuvenation in stem-like cells as much as a mechanism for enhancing resistance in bacteria populations. (biorxiv.org)
  • The EPCs plays critical role in postnatal vasculogenesis through pivotal bioactivities of mobilization, homing, migration, differentiation, and proliferation in angiovasculogenic tissues [ 6 ]. (hindawi.com)
  • The differentiation of endometrial stromal cells into decidual cells, termed decidualization, is an integral step in the establishment of pregnancy. (bioone.org)
  • Gene ontology and upstream regulator pathway analysis showed that WNK1 regulates cell migration, differentiation, and proliferation. (bioone.org)
  • The hallmark of embryonic stem (ES) cells is their ability for self-renewal (capability of unlimited cell division without the loss of pluripotency) as well as for differentiation into all cell types of the adult organism. (uni-muenchen.de)
  • A high expression of Pes1, Bop1 and WDR12 was observed in ES cells, which strongly decreased during in vitro differentiation. (uni-muenchen.de)
  • Enhancer of zeste homolog 2 (EZH2) belongs to poly-sulfur protein family and can mediate cell proliferation and differentiation via the modulation of various genes expressions. (medscimonit.com)
  • Here we analyzed the genome-wide changes in gene transcription occurring during growth and differentiation of porcine primary myoblast cells. (au.dk)
  • While substantial progress has been made to delineate the developmental pathway of T-cell lineage commitment, still little is known about the relationship between differentiation competence and the remarkable expansion of the earliest (DN1 stage) T-cell progenitors. (lu.se)
  • To satisfy differentiation kinetics and overall cell yield data, our models require that adult DN1 cells divide multiple times before becoming competent to progress into DN2 stage. (lu.se)
  • Thus, a liposarcoma appears to arise from a lipoblast but may actually develop through lipoblastic differentiation of a precursor multipotent mesenchymal cell. (medscape.com)
  • it has been hypothesized that dietary calcium may increase prostate cancer risk by reducing circulating levels of 1,25-dihydroxyvitamin D (1,25[OH] 2 D) (10), which promotes the differentiation and inhibits the proliferation of prostate cells (11). (cdc.gov)
  • The SignalStain ® Proliferation/Apoptosis IHC Sampler Kit from Cell Signaling Technology allows the researcher to examine paraffin-embedded tissues or cells with antibodies that will detect cellular apoptosis or proliferation. (cellsignal.com)
  • Each antibody in the SignalStain ® Proliferation/Apoptosis IHC Sampler Kit detects endogenous levels of its target protein and does not cross-react with any related proteins. (cellsignal.com)
  • Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle through inhibiting apoptosis and promoting cell division (4,5). (cellsignal.com)
  • While some assays utilize antibodies to study cell health, proliferation, cell cycle or apoptosis, other types of experiments can rely on non-antibody based methods of assessment, often called non-antibody chemical probes. (biolegend.com)
  • Cellular division for any cell type is dependent on the inherent function, location and the response of cells to repair, apoptosis, or death. (biolegend.com)
  • it inhibits cell migration, reduces the ability to form colonies and induces apoptosis or programmed cell death through the modulation of other key genes (p53, caspase-3, c-PARP) and various apoptotic factors," says Maurizio Battino, coordinator of the research. (agenciasinc.es)
  • It also suppresses the receptor of the epidermal growth factor called EGFR/HER2 and its signalling pathways, which could be an attractive target in cancer therapies because of their important role in the processes of cell survival and proliferation, as well as in those of apoptosis and metastasis. (agenciasinc.es)
  • Here, we explored whether sesamin activates p53, which is widely inhibited in cervical cancer cells, thereby inducing p53-mediated apoptosis. (medsci.org)
  • Cell proliferation, cell cycle distribution, and apoptosis were evaluated by the CCK-8 assay and flow cytometry using PI/Annexin V staining, respectively. (medsci.org)
  • Pifithrin-α also reduced apoptosis and restored the proliferation of HeLa and SiHa cells exposed to sesamin. (medsci.org)
  • These findings indicate that sesamin inhibits cervical cancer cell proliferation, and its mechanism may be attributed to the induction of p53/PTEN-mediated apoptosis. (medsci.org)
  • UCN-01, a protein kinase C (PKC) inhibitor, induces growth arrest and apoptosis in cancer cells and was recently introduced in a phase I clinical trial. (karger.com)
  • The inhibition of cell proliferation was related to arrest of the cell cycle in the G0/G1 phase and increased apoptosis in response to PTGR1 knockdown as indicated by flow cytometry. (go.jp)
  • To discover miR-BARTs that can inhibit apoptosis, I individually expressed miR-BARTs in the EBV- GaCa cell line AGS, and identified five miR-BARTs that conferred this phenotype. (duke.edu)
  • A potential mechanism for the role of calcium in prostate cancer development and progression is that intracellular calcium controls the growth of prostate cancer cells and the process of apoptosis (9). (cdc.gov)
  • In addition, overexpression of miR‑497 was observed to inhibit colony formation and tumor growth in MHCC‑97H human HCC cells. (spandidos-publications.com)
  • Conversely, SMMC‑7721 human HCC cells transfected with a miR‑497 inhibitor exhibited enhanced colony formation and tumor growth. (spandidos-publications.com)
  • Cell proliferation, cell counting, colony formation assay, and Transwell assay were applied to evaluate the proliferation and migration of breast cancer cells. (medscimonit.com)
  • Functional analysis revealed that knockdown of PTGR1 significantly inhibited proliferation and colony formation by PC cells. (go.jp)
  • Our results showed that beginning at four months, nFe2O3-exposed Beas2B underwent neoplastic-like transformation, as indicated by increased cell proliferation and attachment-independent colony formation. (cdc.gov)
  • We isolated stromal cells from endometrial cancer patients to examine local biosynthesis of estrogens and tumor-stromal interactions. (scirp.org)
  • In perspective, this could be particularly relevant in the case of tumor micro-environment diversity, where comprehension of the non-genetic cell heterogeneity could pave the way to novel and more targeted therapies. (biorxiv.org)
  • Thus, unlike other tumor suppressors, RPL5/RPL11 play an essential role in normal cell proliferation, a function cells have evolved to rely on in lieu of a cell cycle checkpoint. (nih.gov)
  • In the present study, it was shown that FBXO17, which is a member of the F-box family, is abnormally downregulated in UCEC tissues compared with non-tumor endometrial tissues, and this was significantly associated with the clinical histological grade, as well as the abnormal proliferation of the UCEC cell line, Ishikawa, both in vitro and in vivo . (medsci.org)
  • New therapeutic concepts in anti-tumor therapy aim to modulate the patient's immune system to increase its aggressiveness or targeted effects toward tumor cells. (frontiersin.org)
  • Treatment of mouse NIH3T3 cells with 5FU, a potent inhibitor of rRNA maturation, confirmed an activation of this checkpoint, leading to weak induction of the tumor suppressor p53, induction of the Cdk-inhibitor p21, an increase in active, hypo-phosphorylated Rb, and to accumulation of cells in the G1- and S-phase with an increase of cells in G1-phase. (uni-muenchen.de)
  • Since hypoxia activates hypoxia-inducible factor (HIF-1)-dependent transcription in cancer cells that, in a paracrine fashion, drive tumor angiogenesis, we investigated the effects of UCN-01 on HIF-1-responsive promoter constructs. (karger.com)
  • Glioma tumor tissues were collected from 3 patients who received surgery, and the glioma stem cells were then separated, cultured, and identified by flow cytometry. (medscimonit.com)
  • however, it also suppresses cancer cell proliferation and recent findings have implicated its role as a tumor suppressor. (oregonstate.edu)
  • The present study revealed that E2F1 gene expression correlates with TS and Survivin gene expressions and tumor proliferation. (aacrjournals.org)
  • Deletions of multiple GID subunits compromise cell proliferation, and this defect is accompanied by deregulation of critical cell cycle markers such as the retinoblastoma (Rb) tumor suppressor, phospho-Histone H3 and Cyclin A. We identify the negative regulator of pro-proliferative genes Hbp1 as a bonafide GID/CTLH proteolytic substrate. (elifesciences.org)
  • This results in the formation of a relatively well-defined zone of compressed fibrous tissue that may contain scattered tumor cells. (medscape.com)
  • We conclude that UCN-01, at clinically relevant concentrations, exerts an anti-neovascularization effect by blocking two important steps in vessel formation: (1) the response of cancer cells to hypoxia, and (2) endothelial cell proliferation. (karger.com)
  • Reduced hippocampal cell proliferation has been previously reported in schizophrenia, which may contribute to hippocampal dysfunction. (brainresearchlab.com)
  • Conclusion: Our results confirm that reduced hippocampal cell proliferation may be present in schizophrenia. (brainresearchlab.com)
  • 2009). Evidence of similarities in the redox control of cell proliferation in animals and plants are provided, particularly glutathione recruitment into the nucleus early in the cell proliferation, which appears to be important in the control of progression through the cell cycle in both kingdoms. (cam.ac.uk)
  • Defects in the control of cell proliferation are a hallmark of cancer, and DNA replication is a key process for cell proliferation. (aacrjournals.org)
  • Mesenchymal cells were isolated from periodontal ligament of impacted third molars (n=2) and cultured on titanium disks (polished and nitrided) and on a plastic surface as a positive control of cell proliferation. (bvsalud.org)
  • By monitoring migratory properties of airway smooth muscle cells in the presence of albuterol isomers, the different effect of albuterol on proliferation and migration of airway smooth muscle cells is probed. (scirp.org)
  • As a kind of well mature pharmaceutical for asthma treatment, it would be very important to distinguish the effect of two different isomers of albuterol on proliferation and migration activities of airway smooth muscle cells. (scirp.org)
  • third, whole tissue culture was also performed to further demonstrate the different effect of R- and S-albuterol on migration of airway smooth muscle cells. (scirp.org)
  • Primary culture of rat airway smooth muscle cells was performed by enzyme digestion. (scirp.org)
  • Stem cells undergo cell proliferation to produce proliferating "transit amplifying" daughter cells that later differentiate to construct tissues during normal development and tissue growth, during tissue regeneration after damage, or in cancer. (wikipedia.org)
  • The results demonstrate that DLEU1 and TRAF4 is highly expressed in GBM tissues and promotes proliferation of GBM cells. (spandidos-publications.com)
  • For example, lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) is highly expressed in GBM tissues and perturbed expression of NEAT1 suppresses cell proliferation and invasion ( 10 ). (spandidos-publications.com)
  • Increased cell proliferation in various tissues and organs leads to the abnormal growth characteristic of Proteus syndrome. (medlineplus.gov)
  • Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and cell proliferation assay were used to explore the functional role of the key lncRNAs in GBM. (spandidos-publications.com)
  • A widely used assay for staining dead cells is Trypan Blue. (dojindo.com)
  • Among the enzyme-based assays, the MTT assay is the best known for determining mitochondrial dehydrogenase activities in living cells. (dojindo.com)
  • Although the 3[H]thymidine-uptake assay is regarded as a reliable cell viability assay, the use of a radioisotope causes various concerns. (dojindo.com)
  • Therefore, CCK-8 is stable for at least 6 months at room temperature and one year at 0-5°C. Since WST-8, WST-8 formazan, and 1-Methoxy PMS have no cytotoxicity in cell culture media, additional experiments may be carried out using the same assay plate. (dojindo.com)
  • The CCK-8 assay involves most of the dehydrogenase in a cell, while MTT only involves mitochondrial dehydrogenase. (dojindo.com)
  • Therefore, the MTT assay depends on mitochondrial activity, not the cell itself. (dojindo.com)
  • Cell Counting Kit-F is another cell viability assay kit based on the esterase activity of viable cells. (dojindo.com)
  • These esterase substrates, therefore, can serve as viability assay probes for cells. (dojindo.com)
  • Clonal formation assay analyzed the change of cell proliferation potency. (medscimonit.com)
  • The effect on tumorigenesis potency of glioma stem cells was determined by mouse transplantation assay. (medscimonit.com)
  • We applied an assay that measures the stability of maintenance of an episomal plasmid in human tissue culture cells to screen for new DNA replication factors. (aacrjournals.org)
  • In addition, intake of nutrients in animals can induce circulating hormones of the Insulin/IGF-1 family, which are also considered growth factors, and that function to promote cell proliferation in cells throughout the body that are capable of doing so. (wikipedia.org)
  • The DEmRNAs were mainly enriched in tumorigenesis‑associated GO terms (angiogenesis, positive regulation of cell proliferation, positive regulation of fibroblast apoptotic processes and regulation of neutrophil migration) and pathways (Hippo signaling pathway, cancer pathways, and Wnt signaling pathway). (spandidos-publications.com)
  • High expression of lncRNA H19 promotes cell migration and enhances angiogenesis ( 11 , 12 ). (spandidos-publications.com)
  • When lncHERG is highly expressed, cell multiplication, invasion and migration are inhibited in GBM ( 16 ). (spandidos-publications.com)
  • However, the effect of albuterol on the proliferation and migration has never been tested. (scirp.org)
  • Since (R)-, but not (S)-albuterol enhances bronchodilation, we expect the two racemic isomers would also affect proliferation and migration of tracheal cells differentially. (scirp.org)
  • R-albuterol, but not S-albuterol, inhibited cell migration. (scirp.org)
  • However, the different effects of R- and S-albuterol on smooth muscle cells proliferation and migration have never been tested. (scirp.org)
  • More important, people found that, during the pathogenesis of asthma, smooth muscle cells could differentiate into the migration phenotype. (scirp.org)
  • This study aimed to investigate the relationship between miR-506 and proliferation and migration of breast cancer cells. (medscimonit.com)
  • Taken together, these data suggest for the first time that, in proliferating rat mesangial cells, B2 receptor stimulation is able to induce, via a pertussis insensitive pathway, the inhibition of tyrosine kinase activity and mesangial cell proliferation. (nih.gov)
  • Therefore, this study proves that Rh4 inhibits cancer cell proliferation by activating the ROS/p53 signaling pathway and activating autophagy to induce ferroptosis, which provides necessary scientific evidence of the great anticancer potential of Rh4. (hindawi.com)
  • Finally, IGF‑1R protein, phosphoinositide 3‑kinase/Akt signaling pathway‑associated proteins and cyclin pathway‑associated proteins were differentially expressed between miR‑497‑overexpressing cells and miR-497-silenced cells. (spandidos-publications.com)
  • Proliferation of the cancer cells was significantly increased through the steroid sulfatase pathway, which metabolizes androgens, estrone sulfate, and estradiol sulfate as its substrates. (scirp.org)
  • They screened thousands of compounds and, after validation and further characterization, they were able to identify an interesting small molecule able to block the pathway in a triple-negative breast cancer cell line(compound 4a in the Figure below). (unige.ch)
  • The scientists then checked whether their compounds could inhibit the Wnt signaling pathway in other triple-negative breast cancer cell lines. (unige.ch)
  • Zheng ZM, Wang YY, Chen M, Yang HL, Lai ZZ, Li MQ, Shao J. FBXO17 Inhibits the Wnt/β-Catenin Pathway and Proliferation of Ishikawa Cells. (medsci.org)
  • Studies have shown that various signaling pathways serve crucial roles in the tumorigenesis of UCEC, amongst which the Wnt/β-catenin pathway is of great interest due to its crucial role in cell proliferation and the huge potential as a therapeutic target. (medsci.org)
  • Besides, the results suggested that FBXO17 may inhibit the Wnt/β-catenin signaling pathway and influence the expression of adhesion molecules, such as E-cadherin and N-cadherin in Ishikawa cells. (medsci.org)
  • Hence, ribosomal stress does not lead to checkpoint activation via the p53-p21-Rb pathway in ES cells. (uni-muenchen.de)
  • On the other hand, in order to identify miR-BARTs that can activate the Wnt signaling pathway, I analyzed the PAR-CLIP data set of C666 cells and discovered nine anti-Wnt signaling targets of miR-BARTs, including seven novel genes and two pro-apoptotic genes identified above. (duke.edu)
  • Diethylstilbestrol regulates mouse gubernaculum testis cell proliferation via PLC-Ca 2+ -CREB pathway. (bvsalud.org)
  • In conclusion, these data suggest that the effects of DES on mouse gubernaculum testis cells are mediated by PLC-Ca2+ -CREB pathway. (bvsalud.org)
  • Our findings provide the first evidence that PLC-Ca2+ -CREB signalling pathway mediates the nongenomic effects of diethylstilbestrol on gubernaculum testis cells . (bvsalud.org)
  • Recently it has been also demonstrated that cellular bicarbonate metabolism, which is responsible for cell proliferation, can be regulated by mTORC1 signaling. (wikipedia.org)
  • We demonstrate that UCN-01, at concentrations lower than those necessary to inhibit cancer cell growth, inhibit proliferation of human endothelial cells in vitro. (karger.com)
  • Thus, suppression of cell proliferation in the hippocampus could constitute one of the mechanisms that underlie depression, and physical activity might be an efficient antidepressant. (biopsychiatry.com)
  • Therefore, our findings show that asymmetric segregation mechanisms can also arise in cancer cell populations, and that, in this case, membrane lipids and mitochondria do not respectively segregate independently from the cytoplasm. (biorxiv.org)
  • In this study, in attempt to provide fresh information about the impact of CS on proliferation of EPCs to elucidate the pathophysiological mechanisms of the diseases related to CS in cellular level, we assessed the proliferation of EPCs after interfering the cells with a series of concentrations of CSE for various times of exposure in vitro . (hindawi.com)
  • IgE was recently shown to induce HASM cell proliferation but the detailed mechanisms remain unknown. (harvard.edu)
  • Collectively, our data provide mechanisms of a novel function of IgE which may contribute, at least in part, to airway remodeling observed in allergic asthma by directly inducing HASM cell proliferation. (harvard.edu)
  • Overall, the results suggest that ES cells use different mechanisms as mature cells to coordinate their proliferation rate with ribosome biogenesis. (uni-muenchen.de)
  • The underlying molecular mechanisms by which androgen-independent PC cells acquire the capacity to proliferate remain largely unclear. (go.jp)
  • These findings suggest that l -citrulline decreases vascular smooth muscle cell proliferation in the A10 cell line by acting on DNA synthesis by mechanisms that involve the ANP receptor. (aspetjournals.org)
  • Other cell-mediated mechanisms (especially inflammation). (cdc.gov)
  • Other cell-mediated mechanisms (especially inflammation induced by TNF-α). (cdc.gov)
  • Here we explore potential mechanisms how the proliferation and contractility of gubernaculum testis cells are regulated by diethylstilbestrol . (bvsalud.org)
  • mitotically active cells of intact segments do not significantly contribute to the blastemal cellular sources. (mdpi.com)
  • Here, we present a method that allows the measurement of asymmetric organelle partitioning, and we use it to simultaneously measure the partitioning of three kinds of cellular elements, i.e. cytoplasm, membrane, and mitochondria in a proliferating population of human Jurkat T-cells. (biorxiv.org)
  • For this porpoise, we use multiple live cell markers which permit us both to follow the partitioning process for multiple generations and to investigate the correlations between the partitioning of different cellular constituents. (biorxiv.org)
  • The method of stimulating isolated cells with CSE in vitro has been explored and frequently applied to determine the direct causes in the relationships between cigarette smoking and cellular functions [ 15 ]. (hindawi.com)
  • Cellular enzymes such as lactate dehydrogenase (LDH), adenylate kinase, and glucose-6-phosphate dehydrogenase are also used as cell death markers, and there are several such products available on the market. (dojindo.com)
  • Cellular redox homeostasis is considered to play an important role in the regulation of the plant cell cycle but there is little information on the precise functions of key redox metabolites such as ascorbate, glutathione and pyridine nucleotides in this process. (cam.ac.uk)
  • Our biochemical and cellular analysis thus demonstrates that the GID/CTLH complex prevents cell cycle exit in G1, at least in part by degrading Hbp1. (elifesciences.org)
  • Cellular proteostasis involves the coordinated and compensatory action of pathways that control biogenesis, folding, trafficking and breakdown of proteins allowing the cell to adapt to physiological or pathological environmental changes. (elifesciences.org)
  • Because of their surface charge, asbestos fibers can adsorb to cellular macromolecules (proteins, DNA, RNA) and cell surface proteins. (cdc.gov)
  • Some evidence suggests that dissolution of IONP following endocytosis into cells may disrupt iron homeostasis, resulting in genotoxicity and neoplastic-like cellular transformation. (cdc.gov)
  • We report here that IgE sensitization induces HASM cell proliferation, as measured by 3H-thymidine, EdU incorporation, and manual cell counting. (harvard.edu)
  • Learn about cell viability assays. (abcam.com)
  • Learn about the assays used to assess cell proliferation and the cell cycle. (abcam.com)
  • Cell viability and cytotoxicity assays are used for drug screening and cytotoxicity tests of chemicals. (dojindo.com)
  • only lactate dehydrogenase does not lose its activity during cell death assays. (dojindo.com)
  • Therefore, cell death assays based on LDH activity are more reliable than other enzyme-based cell death assays. (dojindo.com)
  • WSTs produce water-soluble formazans and are suitable for assays of cell proliferation and cytotoxicity. (dojindo.com)
  • Dehydrogenase-based assays reflect cell conditions with more sensitivity than the other assays because they depend on several elements including dehydrogenases, NAD(H), NADP(H), and mitochondrial activity. (dojindo.com)
  • However, histomorphologic, immunohistochemical, and experimental data suggest that most, if not all, sarcomas arise from primitive, multipotential mesenchymal cells, which in the course of neoplastic transformation differentiate along one or more lines. (medscape.com)
  • Human bronchial epithelial cells (Beas2B) were continuously exposed to nFe2O3 or nano-SiO2 coated nFe2O3 (SiO2-nFe2O3) for up to 6.5 months at an occupationally relevant low dose (0.6 microg/cm2 or 2.88 microg/mL) and evaluated over time for indications of neoplastic-like transformation and its underlying mechanism. (cdc.gov)
  • Anti-androgen flutamide suppresses hepatocellular carcinoma cell proliferation via the aryl hydrocarbon receptor mediated induction of transforming growth factor-β1. (oregonstate.edu)
  • We investigated the AhR-dependent mechanism of action of flutamide in human hepatocellular carcinoma cells and identified that transforming growth factor-β1 (TGF-β1) is induced by flutamide in an AhR-dependent manner. (oregonstate.edu)
  • They arise from cancerous brain and spinal cord glial cells ( 1 , 2 ). (spandidos-publications.com)
  • Neuronal degeneration, edema, glial cell proliferation and perivascular infiltration (7). (cdc.gov)
  • Unexpectedly, we found that their depletion in primary human lung fibroblasts failed to induce cell cycle arrest but strongly suppressed cell cycle progression. (nih.gov)
  • We show that the effects on cell cycle progression stemmed from reduced ribosome content and translational capacity, which suppressed the accumulation of cyclins at the translational level. (nih.gov)
  • Generally, cells have to closely coordinate growth and cell cycle progression during proliferation to prevent premature division. (uni-muenchen.de)
  • Our data demonstrated that in brain glioma cells, the decrease of EZH2 level could suppress cell proliferation and tumorigenesis potency, and meanwhile inhibit the expressions of oncogenes including c-myc and Akt. (medscimonit.com)
  • Hydroquinone, an activator of caspase-9 activity via reactive oxygen species, and farnesol, a post-translational down-regulator of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity suppress the growth of murine B16 melanoma cells. (iiarjournals.org)
  • After mitosis, the cell will undergo the G 0 /G 1 phase when the cells rest to become ready for the next round of replication. (biolegend.com)
  • During myogenesis the satellite cells undergo a series of highly ordered events that include division and proliferation of mononucleated myoblasts, irreversible cell cycle withdrawal, elongation of myoblasts and finally cell-cell fusion, leading to the formation of multinucleated myotubes. (au.dk)
  • These experiments showed that the two DN1 sub-populations divided with similar rates, but progressed to the DN2 stage with different rates, thus providing experimental evidence that DN1 cells increase their commitment probability in a cell-intrinsic manner as they undergo cell division. (lu.se)
  • Proliferation-linked shifts in eligibility of DN1 cells to undergo specification thus control kinetics of T-cell generation. (lu.se)
  • Additionally, the cytotoxicity of MTT formazan makes it difficult to remove cell culture media from the plate wells due to many floating cells with MTT formazan needles, giving significant well-to-well error. (dojindo.com)
  • The role of miR‑497 in regulating the proliferation of HCC‑derived cells was also investigated in vitro and in vivo. (spandidos-publications.com)
  • As an upstream signature component of FcεRI signaling, inhibition of spleen tyrosine kinase (Syk) abrogated the IgE-induced HASM proliferation. (harvard.edu)
  • Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (3). (cellsignal.com)
  • We demonstrated that bradykinin (BK), through the B2 receptor, induced a dose-dependent inhibition of mesangial cell proliferation stimulated by fetal calf serum. (nih.gov)
  • The relationship between cell proliferation, protein tyrosine phosphorylation, phosphotyrosine kinase activity and bradykinin receptor activation in rat mesangial cells was investigated. (nih.gov)
  • This abrogated expression of the decidual marker genes, insulin like growth factor binding protein 1 ( IGFBP1 ) and prolactin ( PRL ), and prevented adoption of decidual cell morphology. (bioone.org)
  • As loss of RPL5/RPL11 abrogates ribosome biogenesis and protein synthesis to the same extent as loss of other essential 60S RPs, we reasoned the loss of RPL5 and RPL11 would induce a p53-independent cell cycle checkpoint. (nih.gov)
  • The proliferation of endothelial cells is detected by MTT method and adhesion of endothelial cells observed by PI fluorescence staining and western-bolt detection of protein of VE-cadherin. (atlantis-press.com)
  • Results: The proliferation of endothelial cells on the titanium surface is better than the culture plate surfaces, the adhesion cells on titanium surface are more numbers, cell morphology is more extended, titanium surface expression of VE-cadherin protein higher than the culture plate surface. (atlantis-press.com)
  • In EBV latently infected epithelial cells, such as nasopharyngeal carcinoma (NPC) and gastric carcinoma (GaCa) cells, viral protein expression is low. (duke.edu)
  • p53 is a key protein that is altered in all cancer cells. (who.int)
  • Mechanistically, we found that U-73122 inhibited DES-induced activation of cAMP- response element binding protein (CREB) in gubernaculum testis cells . (bvsalud.org)
  • 10%. In the remaining 10% of children with lecular weight protein found on the surface nephrotic syndrome, it is largely mediated of all nucleated cells which synthesize it. (who.int)
  • A common trait of antidepressant drugs, electroconvulsive treatment and physical exercise is that they relieve depression and up-regulate neurotrophic factors as well as cell proliferation and neurogenesis in the hippocampus. (biopsychiatry.com)
  • In order to identify possible biological underpinnings of depression and the antidepressant effect of running, we analysed cell proliferation, the level of the neurotrophic factor BDNF in hippocampus and dynorphin in striatum/accumbens in 'depressed' Flinders Sensitive Line rats (FSL) and Flinders Resistant Line (FRL) rats with and without access to running-wheels. (biopsychiatry.com)
  • In the hippocampus, cell proliferation was lower in the 'depressed' rats compared to the control FRL rats but there was no difference in BDNF or dynorphin levels in striatum/accumbens. (biopsychiatry.com)
  • The only parameter that was associated to basal level of 'depression' and to the antidepressant effect was cell proliferation in the hippocampus. (biopsychiatry.com)
  • Ki67-labelled cells were counted within the dentate gyrus and hilus on sections taken from the anterior hippocampus. (brainresearchlab.com)
  • Uncontrolled cell proliferation, leading to an increased proliferation rate, or a failure of cells to arrest their proliferation at the normal time, is a cause of cancer. (wikipedia.org)
  • In mature cells, disruptions in ribosome biogenesis are directly linked to the cell cycle machinery by a p53-dependent activation of the G1/S-phase checkpoint, leading to an arrest of cells in G1-phase. (uni-muenchen.de)
  • Impaired ribosome biogenesis is known to activate a p53-dependent checkpoint in mature cell lines, which leads to an arrest of cells in G1-phase. (uni-muenchen.de)
  • The growth-suppression effected by biphenylpropyl-O-acetylhydroquinone was accompanied by a dose-dependent arrest at the G1/S interface of the cell cycle, an impact greater than that previously reported for farnesyl-O-acetylhydroquinone (IC 50 =2.5 μmol/L). These new hydroquinone derivatives may have potential in cancer chemoprevention and/or therapy. (iiarjournals.org)
  • We find that the DDX5 locus is frequently amplified in breast cancer and that breast cancer-derived cells with amplification of DDX5 are much more sensitive to its depletion than breast cancer cells and a breast epithelial cell line that lacks DDX5 amplification. (aacrjournals.org)
  • 95% of the global population, and can give rise to a number of malignancies in B cells and epithelial cells. (duke.edu)
  • It is likely that miR-BARTs play a crucial role in EBV-infected epithelial cells, however a comprehensive understanding of miR-BARTs is currently lacking. (duke.edu)
  • Diethylstilbestrol (DES) is a nonsteroidal synthetic estrogen that disrupts the morphology and proliferation of gubernaculum testis cells , but the underlying mechanism is unclear. (bvsalud.org)
  • During the progression of NSCLC, E2F1 overexpression could produce more aggressive tumors with a high proliferation rate and chemoresistance. (aacrjournals.org)
  • The critical challenge in cancer treatment is how to effectively kill cancer cells without affecting healthy cells. (hindawi.com)
  • Next we examined the effects of steroid sulfatase inhibitor and aromatase inhibitor in monoculture of endometrial cancer cell line (Ishikawa) and in a co-culture system involving an Ishikawa cells and stromal cells. (scirp.org)
  • Estrogen receptor and steroid sulfatase mRNA levels in cancer cells were significantly higher in the co-cultures compared with the monocultures of endometrial cancer cells. (scirp.org)
  • We evaluated the effects of STS inhibitor and aromatase inhibitors on the proliferation of estrogen-dependent endometrial cancer cells. (scirp.org)
  • Whether the same processes take place in the context of cancer cell lines is still poorly investigated. (biorxiv.org)
  • This helps us understand the high phenotypic variability reported in these cancer cell lines. (biorxiv.org)
  • Spanish and Italian researchers have proven that when honey from strawberry trees, a product typical of Mediterranean areas, is added to colon cancer cells grown in the laboratory, cell proliferation is stopped. (agenciasinc.es)
  • None of these effects was observed when honey was applied to healthy cell lines," the authors point out, noting the anticarcinogenic potential of this natural product, but insisting on the need for new studies with colon cancer in vivo models to confirm its chemopreventive effects. (agenciasinc.es)
  • Human HeLa and SiHa cervical cancer cells and normal Hs68 dermal cells were used as cell models. (medsci.org)
  • MiR-506 mimics, inhibitor, and negative control (NC) were transfected into MDA-MB-231 breast cancer cells. (medscimonit.com)
  • We report that, in addition to direct inhibitory effects on endothelial cell growth, UCN-01 abrogates hypoxia-mediated transactivation of HIF-1-responsive promoters in a prostate cancer cell line. (karger.com)
  • We found that flutamide inhibited the growth of several cancer cell lines independent of AR status, and that suppression of AhR expression reversed the anti-proliferative effects of flutamide. (oregonstate.edu)
  • We investigated the clinical significance of E2F1 gene expression in relation to its target genes, thymidylate synthase ( TS ) and Survivin , in case of non-small-cell lung cancer (NSCLC). (aacrjournals.org)
  • Lung cancer is a major cause of cancer-related deaths, and non-small-cell lung cancer (NSCLC) comprises ∼75% of all lung cancers ( 1 ). (aacrjournals.org)
  • Our results show a novel role for DDX5 in cancer cell proliferation and suggest DDX5 as a therapeutic target in breast cancer treatment. (aacrjournals.org)
  • DDX5 is required for cell proliferation by controlling the transcription of genes expressing DNA replication proteins in cancer cells in which the DDX5 locus is amplified, and this has uncovered a dependence on DDX5 for cell proliferation. (aacrjournals.org)
  • Given the high frequency of DDX5 amplification in breast cancer, our results highlight DDX5 as a promising candidate for targeted therapy of breast tumors with DDX5 amplification, and indeed we show that DDX5 inhibition sensitizes a subset of breast cancer cells to trastuzumab. (aacrjournals.org)
  • Understanding how DNA replication is regulated in human cells can provide insight into cancer development and may reveal vulnerabilities that can be exploited therapeutically. (aacrjournals.org)
  • This may have an important impact in understanding the biology of wild-type p53 in cancer-transformed cells. (who.int)
  • Cancer is an unregulated proliferation of cells. (msdmanuals.com)
  • Cell proliferation leads to an exponential increase in cell number and is therefore a rapid mechanism of tissue growth. (wikipedia.org)
  • We found that although cell cytoplasm is divided symmetrically, mitochondria and membrane lipids are asymmetrically distributed between the two daughter cells and present a stable positive correlation with cytoplasm apportioning, which is incompatible with an independent division mechanism. (biorxiv.org)
  • To observe the proliferation and adhesion of umbilical cord endothelial cells (HUVEC) on the titanium (Ti) surface with large particles blasted-etched (SLA) treatments and further clarify the mechanism of action of each other between the Ti implant surface and the body. (atlantis-press.com)
  • It is produced by Idiopathic nephrotic syndrome (INS) a variety of cells, including monocytes and accounts for 90% of nephrosis in child- mesangial cells in the kidney [ 9 ]. (who.int)
  • Sample suitability indicates whether this reagent can be used to label already fixed tissue sections or single cells in suspension or cell culture. (biolegend.com)
  • There can be symmetrical or asymmetrical division depending on the cell and tissue type. (biolegend.com)
  • A fat cell is an animal connective tissue cell specialized for the synthesis and storage of fat. (mcw.edu)
  • Method: We measured the cell proliferation marker, Ki67, in post-mortem hippocampal tissue from patients with schizophrenia ( n = 10) and matched controls ( n = 16). (brainresearchlab.com)
  • The presence of asbestos fibers in the lungs sets off a variety of responses leading to inflammation, cell, and tissue damage, which can lead to malignant and non-malignant diseases. (cdc.gov)
  • Recent evidence suggests that IgE sensitization can enhance the survival and mediator release in inflammatory cells. (harvard.edu)
  • In vivo development and survival of NK cells require cytokines ( 6 - 8 ). (frontiersin.org)
  • Inhibition of p53 by pifithrin-α significantly reduced the levels of PUMA, Bax, and PTEN but restored AKT phosphorylation in SiHa cells exposed to sesamin. (medsci.org)
  • Treatment with honey stops the tumour cells cycle by regulating some genes (cyclin D1, CDK2, p27Kip. (agenciasinc.es)
  • To identify pro-apoptotic genes targeted by the five anti-apoptotic miRNAs, I validated one previously published target and identified nine novel targets by performing photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) in the EBV+ NPC cell line C666. (duke.edu)
  • We determine a comprehensive map of lineage-specific lncRNAs in human dermal lymphatic and blood vascular endothelial cells (LECs and BECs), combining RNA-Seq and CAGE-Seq. (nature.com)
  • During development, the blood vascular system arises from endothelial cell progenitors that differentiate from mesodermal cells, mostly through the expression of the transcription factor (TF) ETV2. (nature.com)
  • Activation of the VEGFA/VEGFR2 signaling and expression of blood vascular endothelial cell (BEC) markers, such as NRP1 and EphrinB2, further differentiate these precursor cells into BECs, which then form the hierarchical network of blood vessels 4 . (nature.com)
  • However, direct injection of IL-2 has been shown to be accompanied by severe side effects, such as vascular leak syndrome, activation-induced cell death, and strong induction of regulatory CD4 pos T cells, which did not occur after IL-15 administration ( 15 , 16 ). (frontiersin.org)
  • Damage to the endothelium results in vascular smooth muscle cell proliferation. (aspetjournals.org)
  • In this work, we investigated the effect of l -citrulline on vascular smooth muscle cell proliferation. (aspetjournals.org)
  • l -Citrulline (10 −8 M) significantly decreased rat aortic (A10 cell line) vascular smooth muscle proliferation. (aspetjournals.org)
  • Cell division can occur without cell growth, producing many progressively smaller cells (as in cleavage of the zygote), while cell growth can occur without cell division to produce a single larger cell (as in growth of neurons). (wikipedia.org)
  • Endothelial progenitor cells (EPCs) contribute to neovascularization and play an important role in the development of these diseases. (hindawi.com)
  • In conclusion, PTGR1 plays an essential role in PC cells and may be a potential therapeutic target for PC. (go.jp)
  • may play a role in eliminating early cancers or premalignant cells. (msdmanuals.com)
  • Calcein-AM is used for the kit because it is retained inside of cells better than the other fluorescent esterase substrates. (dojindo.com)
  • The results, published in the Journal of Functional Foods, show that strawberry tree honey is capable of inhibiting the proliferation of tumour cells cultivated in laboratory plates - more specifically, those of a line of human colon adenocarcinoma (HCT-116) and another with metastatic characteristics (LoVo), widely used to investigate this type of tumour. (agenciasinc.es)
  • This led to other relevant results, such as the reduction of some transcription factors, the inhibition of the activity of certain enzymes and the decrease of mitochondrial respiration and glycolysis of tumour cells. (agenciasinc.es)
  • Results: We replicated the finding of a significant reduction in Ki67+ cells/mm 2 in schizophrenia cases compared to controls ( t 24 = 2.1, p = 0.023). (brainresearchlab.com)
  • Our results demonstrated high potential of IL-15 for NK cell expansion, while IL-21 triggered NK cell maturation and functionality. (frontiersin.org)
  • The results demonstrated that U-73122 impaired DES-evoked intracellular Ca2+ mobilization in gubernaculum testis cells and inhibited DES-induced proliferation of gubernaculum testis cells . (bvsalud.org)
  • Strawberry tree honey is capable of inhibiting the proliferation of tumour cells on a line of human colon adenocarcinoma (HCT-116) and another with metastatic characteristics (LoVo). (agenciasinc.es)
  • Here, we investigate the effects of the photovoltaic activity of p-i-n nanowire arrays on A549 human lung adenocarcinoma cells. (lu.se)
  • Primary human endometrial stromal cells (HESCs) were subjected to small interfering RNA knockdown of WNK1 followed by in vitro decidualization. (bioone.org)
  • Human ASM (HASM) cells express both low affinity (FcεRII/CD23) and high affinity IgE Fc receptors (FcεRI), and IgE can modulate the contractile and synthetic function of HASM cells. (harvard.edu)
  • To evaluate the proliferative capacity of mesenchymal cells derived from human periodontal ligament on polished and plasma-treated titanium surfaces. (bvsalud.org)
  • We concluded that plasma nitriding produced surfaces that permitted the proliferation of human periodontal ligament mesenchymal cells. (bvsalud.org)
  • One important factor for cell growth is ribosome biogenesis. (uni-muenchen.de)
  • During this work, the function of the proteins Pes1, Bop1 and WDR12, which were shown previously to be involved in ribosome biogenesis of mature cell lines, was investigated in mouse ES cells. (uni-muenchen.de)
  • Exposure to radiation can damage the basal cell layer of the skin and result in inflammation, erythema, and dry or moist desquamation. (cdc.gov)
  • Zaprinast, a phosphodiesterase type V inhibitor, enhanced 5-bromo-2′-deoxyuridine incorporation in serum-stimulated cells. (aspetjournals.org)
  • In this study, mouse gubernaculum testis cells were pretreated with phospholipase C (PLC) inhibitor U-73122 and then treated with DES. (bvsalud.org)
  • Conclusion: The SLA Ti surface can promote the proliferation and adhesion of endothelial cell and could promote the early healing process of osseointegration. (atlantis-press.com)
  • GSH accumulation in the nucleus is triggered in Arabidopsis cells in culture by treatments that synchronize cells at G1/S as identified by flow cytometry and marker transcripts. (cam.ac.uk)
  • Sesamin (75 and 150 μM) clearly inhibited SiHa and HeLa cell proliferation in a dose-dependent fashion, but did not affect the proliferation of Hs68 cells. (medsci.org)
  • Perillyl-O-acetylhydroquinone, biphenylethyl-O-acetylhydroquinone and biphenylpropyl-O-acetylhydroquinone had dose-dependent impacts on the proliferation of B16 cells with 50% inhibitory concentration (IC 50 ) values of 8.0, 4.2 and 1.4 μmol/L, respectively. (iiarjournals.org)