• genes
  • STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. (wikipedia.org)
  • Increased activity of STAT3 in cancer cells, leads to changes in the function of protein complexes that control expression of inflammatory genes, with result profound change in the secretome and the cell phenotypes, their activity in the tumor, and their capacity for metastasis. (wikipedia.org)
  • The murine double minute (mdm2) oncogene, which codes for the Mdm2 protein, was originally cloned, along with two other genes (mdm1 and mdm3) from the transformed mouse cell line 3T3-DM. (wikipedia.org)
  • Removing MDM2 simultaneously with the H2AK119 E3 ligase Ring1B/RNF2 further induced these genes and synthetically arrested cell proliferation. (wikipedia.org)
  • Viruses offer an efficient means of delivering genes into cells, which they evolved to do, and are thus of great use as experimental tools. (wikipedia.org)
  • This danger can be avoided by the use of viruses which lack key genes, and which are thus unable to replicate after entering a cell. (wikipedia.org)
  • In order to propagate such viral vectors, a cell line that expresses the missing genes is required. (wikipedia.org)
  • Since HEK 293 cells express a number of adenoviral genes, they can be used to propagate adenoviral vectors in which these genes (typically, E1 and E3) are deleted, such as AdEasy. (wikipedia.org)
  • Indeed, cancer cells can become addicted to the transcriptional silencing, due to promoter hypermethylation, of some key tumor suppressor genes, a process known as epigenetic addiction. (wikipedia.org)
  • These ras genes have GTP/GDP binding and GTPase activity, and their normal function may be as G-like regulatory proteins involved in the normal control of cell growth. (wikipedia.org)
  • proteins
  • To identify EMT-related breast cancer therapeutic targets and biomarkers, we have used two proteomic approaches to find proteins that change in abundance upon the induction of EMT by TGF-β in two mouse mammary epithelial cell lines, NMuMG and BRI-JM01. (gc.ca)
  • Results: Preliminary experiments based on two-dimensional electrophoresis of a hydrophobic cell fraction identified only 5 differentially expressed proteins from BRI-JM01 cells. (gc.ca)
  • Using these approaches, we identified several proteins that are modulated during the EMT process, including cell adhesion molecules (several members of the Integrin family, Fibronectin, Activated leukocyte cell adhesion molecule, and Neural cell adhesion molecule 1) and regulators of cellular signaling (Tumor-associated calcium signal transducer 2, Basigin). (gc.ca)
  • I can say, that a colleague, has stablyintegrated 5 different proteins in the same cell and all 5 are expressing themselves correctly. (protocol-online.org)
  • cells I've done this on: U87 already transfected with 2 extra (selected) proteins and Hela cells already transfected with 2 proteins and 2 other sequences, thus a total of 4 different pieces of separate DNA integrated stably). (protocol-online.org)
  • Many cell surface proteins in mammalian cells are anchored to the plasma membrane via glycosylphosphatidylinositol (GPI). (biomedsearch.com)
  • Indeed, cells defective in dihydroxyacetone phosphate acyltransferase (DHAP-AT) or alkyl-DHAP synthase express only the diacyl form of GPI-anchored proteins. (biomedsearch.com)
  • Examples of such experiments include: Effects of a drug on sodium channels Inducible RNA interference system Isoform-selective protein kinase C agonist Interaction between two proteins Nuclear export signal in a protein A more specific use of HEK 293 cells is in the propagation of adenoviral vectors. (wikipedia.org)
  • Actins are highly conserved proteins that are involved in cell motility, structure and integrity. (wikipedia.org)
  • apoptosis
  • Ionizing radiation (IR) activates TGFβ1 through a redox mechanism (Radiat Res 166:839-48, 2006) Our recent studies show that TGFβ1 depletion in irradiated epithelial cells compromises ataxia telengiectasia mutated (ATM) kinase activity and autophosphorylation, leading to reduced phosphorylation of critical DNA damage transducers γH2AX, Chk2, p53 and Rad17, abrogation of the cell cycle block and apoptosis (Cancer Res 66 (22):10861, 2006). (aacrjournals.org)
  • This benzophenanthridine alkaloid can induce apoptosis in some transformed or malignant cell lines. (wikipedia.org)
  • genome
  • Polyploid giant cancer cells (PGCCs) are mononucleated or multinucleated cells with multiple genome copies that can be observed in cancer but that can also play an important role in many normal physiologic and nonmalignant pathologic processes [ 10 - 12 ]. (pubmedcentralcanada.ca)
  • In normal cells, CpG islands preceding gene promoters are generally unmethylated, and tend to be transcriptionally active, while other individual CpG dinucleotides throughout the genome tend to be methylated. (wikipedia.org)
  • citation needed] The entire genome of a cancerous cell contains significantly less methylcytosine than the genome of a healthy cell. (wikipedia.org)
  • In fact, cancer cell genomes have 20-50% less methylation at individual CpG dinucleotides across the genome. (wikipedia.org)
  • Cytokines
  • In response to cytokines and growth factors, STAT3 is phosphorylated by receptor-associated Janus kinases (JAK), form homo- or heterodimers, and translocate to the cell nucleus where they act as transcription activators. (wikipedia.org)
  • HeLa
  • In this work we have used Fourier transform infrared (FTIR) / vibrational absorption (VA) spectroscopy to study two cancer cell lines: the Henrietta Lacks (HeLa) human cervix carcinoma and 5637 human bladder carcinoma cell lines. (dtu.dk)
  • We have been able to identify certain bands in the spectra which are so-called biomarkers for two types of cell lines, three groups for the 5637 human bladder carcinoma cell line (5637A, 5637B and 5637C), and another one for the HeLa human cervix carcinoma cell line. (dtu.dk)
  • clones
  • Transformed clones were obtained only with the carcinogen. (sciencemag.org)
  • Chronically infected cell lines and clones are currently used as sources of virus for infecting other cell types, as "factories" for large‐scale production of virions or viral components, and as model systems for studies of regulation of virus expression. (currentprotocols.com)
  • oncogene
  • In this study, we established a mouse epithelial cancer cell line, designated as Chinese University Papillomavirus-1 CUP-1, from C57BL-KsJ mice through persistent expression of HPV-16 E7 oncogene. (duhnnae.com)
  • However, in cancer cells, CpG islands preceding tumor suppressor gene promoters are often hypermethylated, while CpG methylation of oncogene promoter regions and parasitic repeat sequences is often decreased. (wikipedia.org)
  • cancers
  • Most human breast cancers are reported to be refractory to transforming growth factor beta 1 (TGFβ1) mediated growth regulation while producing large amounts of TGFβ1 (reviewed in Cancer Metastasis Rev 20 (1-2):133, 2001). (aacrjournals.org)
  • Activating mutations of the Ras protein and upstream elements of the Ras protein may play a role in more than two thirds of all human cancers, including most metastatic disease, which suggests that Reolysin may be an effective therapeutic for many Ras-activated tumor types and potentially for some cell proliferative disorders. (wikipedia.org)
  • differentiation
  • STAT3 is essential for the differentiation of the TH17 helper T cells, which have been implicated in a variety of autoimmune diseases. (wikipedia.org)
  • Biology
  • HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection. (wikipedia.org)
  • He has held academic appointments and tenure at the University of North Carolina School of Medicine (Assistant and Associate Professor of Pharmacology and Program Leader of the Cell Biology Program at the Lineberger Cancer Center), Vanderbilt University School of Medicine (Professor of Cell Biology) and the University of South Florida College of Medicine (Professor of Biochemistry and Oncology). (wikipedia.org)
  • He has over 150 publications in journals, including Science, Nature, Proceedings of the National Academy of Sciences, USA, Molecular Cell Biology, and Journal of Cell Biology. (wikipedia.org)
  • Mol Cell Biology. (wikipedia.org)
  • Alan Hall FRS (19 May 1952 - 3 May 2015) was a British cell biologist and a biology professor at the Sloan-Kettering Institute, where he was chair of the Cell Biology program. (wikipedia.org)
  • In 1993 he moved to University College London, where he helped to create a new MRC centre for molecular cell biology. (wikipedia.org)
  • Infection
  • The use of cell lines as targets of acute infection is also described. (currentprotocols.com)
  • This cycle of infection, replication and cell death is believed to be repeated until all tumour cells carrying an activated Ras pathway are destroyed. (wikipedia.org)
  • During viral infection, mice lacking STAT3 in T-cells display impairment in the ability to generate T-follicular helper (Tfh) cells and fail to maintain antibody based immunity. (wikipedia.org)
  • neural
  • Apart from its role in cell cycle control, the DREAM complex has been shown to be involved in the programmed cell death of neural precursor cells in Drosophila melanogaster. (wikipedia.org)
  • Molecular
  • Warren Jackson (Jack) Pledger is a molecular cell biologist who is the Associate Director for Research and Deputy Director of the Gibbs Cancer Research Institute in Spartanburg, South Carolina. (wikipedia.org)
  • inhibition
  • Therefore, we hypothesized that TGFβ inhibition might increase clonogenic cell death in irradiated cancer cells. (aacrjournals.org)
  • Non-malignant MCF10A, breast cancer cell line Hs578T and lung carcinoma A549 were found to be responsive to TGFβ1 mediated growth inhibition while MCF7, MDAMB231 and T47D breast cancer cell lines were refractory. (aacrjournals.org)
  • Selective inhibition of immunoregulatory cell functions by Cyclosporin A. J. Immunol. (currentprotocols.com)
  • His current projects address the impact of cell cycle inhibition on tumor cell survival, and the hallmarks of cancer during the evolution of tumor progression seeking to find new therapeutic avenues. (wikipedia.org)
  • mutations
  • Here, we report that GPI lipid remodeling is defective in cells from patients with Zellweger syndrome having mutations in the peroxisomal biogenesis factors PEX5, PEX16, and PEX19 and in cells from patients with RCDP types 1, 2, and 3 caused by mutations in PEX7, DHAP-AT, and alkyl-DHAP synthase, respectively. (biomedsearch.com)
  • An eight-amino acid residue (326-333) in actin has been shown to mediate the interaction between actin and eNOS Recurrent mutations in this gene have been associated to cases of diffuse large B-cell lymphoma. (wikipedia.org)
  • clonogenic
  • We then treated the cell lines with TGFβ1 type kinase I small molecule inhibitor 48 hr prior to, and 3 hr post, graded doses of radiation in serum-replacement media before determining clonogenic survival. (aacrjournals.org)
  • Inhibiting TGFβ signaling in MCF10A, MCF7, MDAMB231, T47D and Hs578T cells significantly increased radiosensitivity as measured by clonogenic cell death. (aacrjournals.org)
  • pathway
  • It has since been shown to replicate specifically in cells that have an activated Ras pathway with very little effect in cells that do not have active Ras pathways. (wikipedia.org)
  • activity
  • The product encoded by this gene is predominantly localized in the nucleus and is a cell division cycle protein with kinase activity. (wikipedia.org)
  • Although expression levels of the protein appear to be constant throughout the cell cycle, the protein kinase activity appears to increase during S phase. (wikipedia.org)
  • Results showed no correlation between the wild-type or mutant N-ras p21's GTPase activity and transforming potential. (wikipedia.org)
  • progression
  • It has been suggested that the protein is essential for initiation of DNA replication and that it plays a role in regulating cell cycle progression. (wikipedia.org)
  • nucleus
  • This gene encodes a histone H1 binding protein that is involved in transporting histones into the nucleus of dividing cells. (wikipedia.org)
  • In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa. (wikipedia.org)
  • kinase
  • Cell division cycle 7-related protein kinase is an enzyme that in humans is encoded by the CDC7 gene. (wikipedia.org)
  • The Cdc7 kinase is involved in regulation of the cell cycle at the point of chromosomal DNA replication. (wikipedia.org)
  • this means that most eukaryotic cells have the Cdc7 kinase protein. (wikipedia.org)
  • The gene, CDC7, is involved in the regulation of cell cycle because of the gene product Cdc7 kinase. (wikipedia.org)
  • infect
  • Reovirus replicates in and eventually kills Ras-activated tumour cells, and as cell death occurs, progeny virus particles are then free to infect surrounding cancer cells. (wikipedia.org)
  • growth factor
  • He demonstrated that growth factors controlled cell cycle regulatory events and has actively studied these processes, including platelet derived growth factor (PDGF) and insulin-like growthfactor-1 (IGF-1) signal transduction. (wikipedia.org)
  • normal
  • Background: TGF-β acts as an antiproliferative factor in normal epithelial cells and at early stages of oncogenesis. (gc.ca)
  • The comparison with the normal cells showed that Rac1 stimulates actin filament production at the membrane, pinocytosis, and membrane ruffling. (wikipedia.org)