• fibroblast
  • 21:393-425 comment cells from the lung of a young male Chinese hamster (Cricetulus griseus) The ECACC catalog 1993 (Hypercard stack for Macintosh computers) gives the following spec ECACC number 86041102 cell name V79 description hamster lung morphology fibroblast depositor Wynford-Thomas, U of Wales, College of Med. (bio.net)
  • Zebrafish AB9 cells are a primary fibroblast cell line developed from fin tissue of the AB strain. (wikipedia.org)
  • endothelial cell
  • Hello all- I am in need of a good all around human endothelial cell line for use in viral transformation studies, as well as studies of certain viral genes. (bio.net)
  • ATCC hTERT immortalized dermal microvascular endothelial cells have an extended lifespan, express a panel of endothelial cell surface proteins, undergo tubule formation in culture and are karyotypically, morphologically, and phenotypically similar to the primary parent cells. (atcc.org)
  • fetal
  • Cells are grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 15% fetal bovine serum (FBS) in a humid, 5% CO2-enriched atmosphere at 28 °C. Under these conditions, cells passaged 1 in 4 doubled every 72 hours when fed with fresh culture medium at 3-day intervals. (wikipedia.org)
  • Based on this, Hayflick was given another line of fetal cells to try to preserve and duplicate using this method. (wikipedia.org)
  • HeLa
  • The origins of some immortal cell lines, for example HeLa human cells, are from naturally occurring cancers. (wikipedia.org)
  • Major examples include human HeLa cells that were obtained from a cervical cancer, mouse Raw 264.7 cells that were subjected to mutagenesis and then selected for cells which are able to undergo division. (wikipedia.org)
  • Contaminated cell lines should never be used for research demanding the specific type of cell line they are assumed to be, and most of them should ideally be discarded or at least not used in research at all, except when the contaminant cells have acquired novel characteristics (e.g., by mutation or viral transfection, for example the HeLa derivate Det98) and thus constitute a novel lineage after all. (wikipedia.org)
  • It is worth noting that the widespread contamination with HeLa cells was initially recognized by Walter Nelson-Rees using simple Giemsa stain karyotyping under a light microscope. (wikipedia.org)
  • This technique works well in recognizing HeLa because these cells have distinctive chromosome aberrations. (wikipedia.org)
  • It is good practice to periodically check cell lines maintained under laboratory conditions (i.e., not placed in long-term storage) for contamination with HeLa or other common contaminants, to ensure that their quality and integrity is maintained. (wikipedia.org)
  • WI-38 was invaluable to early researchers, especially those studying virology and immunology, since it was a readily-available cell line of normal human tissue, unlike the HeLa line, which were cancerous cells. (wikipedia.org)
  • gene
  • 1. Does anybody have a set protocol that describes the procedure starting from trancfecting cells to performing gene expression studies using stably transfected cells? (bio.net)
  • citation needed] Introduction of a viral gene that partially deregulates the cell cycle (e.g., the adenovirus type 5 E1 gene was used to immortalize the HEK 293 cell line). (wikipedia.org)
  • The zebrafish fibrolast cell line AB9 as a tool to complement gene regulation studies" (PDF). (wikipedia.org)
  • For offline access, many of our popular cell lines that are usually available for immediate delivery are listed in our downloadable catalog - your gene edited cell line may have already been created! (horizondiscovery.com)
  • Glial cell-derived neurotrophic factor (GDNF) is a protein that, in humans, is encoded by the GDNF gene. (wikipedia.org)
  • 1977
  • Further, Shah and Beckett reported the use of differentially pumped cells or chambers to presumably maintain botanical specimens conductive in order to allow the use of the absorbed specimen current mode for signal detection in 1977 and in 1979. (wikipedia.org)
  • reported the design and use of various environmental cell detection configurations in an SEM including differential pumping, or the use of electron transparent films to maintain the specimens in their wet state in 1977. (wikipedia.org)
  • 1999
  • A Holstein heifer named Daisy was cloned by Dr. Xiangzhong (Jerry) Yang using ear skin cells from a high-merit cow named Aspen at the University of Connecticut in 1999, followed by three additional clones, Amy, Betty, and Cathy in 1999. (wikipedia.org)
  • 2002
  • A project is currently underway to enumerate and rename contaminated cell lines to avoid errors in research caused by misattribution (Masters, 2002). (wikipedia.org)
  • 2002). Contaminated cell lines have been extensively used in research without knowledge of their true character. (wikipedia.org)
  • neurons
  • Antibiotic & Antimycotic Their embryological origin with neuroblastic cells means they can easily differentiate into neuron-like cells even though they are not considered adult neurons. (wikipedia.org)
  • tissue
  • The alternative, performing an analysis on primary cells from multiple tissue donors, does not have this advantage. (wikipedia.org)
  • His research interests include metabolic network modelling, systems biology, tissue engineering and cell culture, the development of analysis procedures for genome-scale models, and the experimental verification of these models in Escherichia coli, Saccharomyces cerevisiae and other important organisms. (wikipedia.org)
  • WI-38 is a diploid human cell culture line composed of fibroblasts derived from lung tissue of a 3 months gestation female fetus. (wikipedia.org)
  • tumour
  • Hi cell biologists, I am looking for tumour cell lines derived from childhood renal tumours such as Wilms' or the bone marrow metastasizing renal tumour of chilhood (BMRTC). (bio.net)
  • stable
  • My goal is to transfect these cells with GFP reporter plasmids and establish stable cell lines. (bio.net)
  • We are trying to establish two stable cell lines using G418 resistance and hygromycin resistance. (bio.net)
  • Could you still establish a stable cell line? (bio.net)
  • Baden HP, Kubilus J, Wolman SR, Steinberg ML, Phillips SB, Kvedar JC (1987) The NM 1 keratinocyte line is cytogenetically and biologically stable and exhibits a unique structural protein. (springer.com)
  • ATCC hTERT immortalized aortic endothelial cells have an extended lifespan with a stable karyotype. (atcc.org)
  • ATCC hTERT immortalized Barrett's esophageal epithelial cells contain stable, defined cell cycle and genetic abnormalities, have an extended life span, and are karyotypically, morphologically, and phenotypically similar to the primary parent cells. (atcc.org)
  • ATCC hTERT immortalized bronchial epithelial cells have an extended lifespan, consistently form an electrically tight epithelium, have a stable karyotype and are phenotypically similar to the primary parent cells. (atcc.org)
  • mycoplasma
  • non-specific killing of our targets despite the fact that we use T-cell (1) Have you tested for mycoplasma? (bio.net)
  • receptors
  • The hybridoma D0-11.10, a mouse monoclonal antibody against receptors for antigen plus I region products on T cells. (nationaljewish.org)
  • Hybridomas expressing various combinations of gamma delta T cell receptors available as research tools. (nationaljewish.org)
  • commonly
  • They can restore the response of purified T lymphocytes to Con A. They show increased CO2 production on phagocytosis and can be differentiated into macrophage-like cells using for example phorbol 12-myristate 13-acetate (commonly known as PMA or TPA). (wikipedia.org)
  • These cells are commonly used for studies focusing on the biochemical and molecular properties of zebrafish. (wikipedia.org)
  • malignant
  • In order to provoke malignant conversion HaCaT cells were transfected with mutated cellular Ha-ras (val-12). (springer.com)
  • fibroblasts
  • ATCC hTERT immortalized chondrocyte fibroblasts have an extended lifespan, and are karyotypically, morphologically, and phenotypically similar to the primary parent cells. (atcc.org)
  • densities
  • I always plate freshly thawed cells at very high densities (unless there is a good reason not to) to get the fastest recovery from the freeze. (bio.net)
  • culture
  • After considering starting cell culture of astrocytes does anyone have any comments as to the usefulness of bought cell lines such as the c6 glioma line? (bio.net)
  • Experiments using cell culture are used for developing new diagnostic tests and new treatments for diseases. (wikipedia.org)
  • ATCC
  • 32:857- ATCC designat CCL 16 comments derived from 8 motn old male adult How many different V79 cell lines are there? (bio.net)
  • The B103 and B104 lines have appeared in the literature recently, but I am unable to locate the lines at ATCC, JCTA, etc. (bio.net)
  • growth
  • PC12 cells stop dividing and terminally differentiate when treated with nerve growth factor or dexamethasone. (wikipedia.org)
  • Treatment of PC12 cells with nerve growth factor creates cells with long processes known as neurite varicosities, which contain small amounts of vesicles. (wikipedia.org)
  • PC12 cells treated for 10-14 days with nerve growth factor had no release of vesicles from the cell body which indicates the aggregation of vesicles in the ends of the neurites. (wikipedia.org)
  • Cells that are fairly density dependent for growth (NIH 3T3 cells, for ex. (bio.net)
  • The derived cell clones could be classified according to their growth properties in vivo into one (1) non-tumorigenic (growing as surface epithelia) and two tumorigenic groups, forming either (2) benign cysts or (3) well-differentiated squamous cell carcinomas. (springer.com)
  • Working in viruses of aquaculture we have a number of raggedy primary monocultures that we have been tiptoeing around in the hopes of getting a cell line for routine use in screening.Are there any methods involving growth enhancing chemicals or carcinogens that may help speed this process up? (bio.net)
  • Because of slow growth rates and easy detachment of the original NCI-H295 strains, efforts were made to select a population of cells with better monolayer attachment and more rapid growth. (wikipedia.org)
  • undergo
  • This finding would later be known as the Hayflick limit, which indicated that normal human cells could only undergo a limited number of divisions, and later contributed to the discovery of the biological roles of telomeres. (wikipedia.org)
  • ECACC
  • At ECACC the STR PCR profile of all stocks of this cell line match the profile of the original source material received at ECACC. (sigmaaldrich.com)
  • researchers
  • In 2001 researchers at Advanced Cell Technology in Worcester, Massachusetts, United States, reported that 24 successfully cloned Holsteins had been monitored from birth to the age of four. (wikipedia.org)
  • viable
  • Also, if your freeze killed a lot of the cells, the viable cell plating density after thawing is even lower. (bio.net)
  • However, during this period of research, the team also discovered that if cells were properly stored in a freezer, cells would remain viable and could provide enormous numbers of cells for research purposes. (wikipedia.org)
  • characteristics
  • Boukamp P, Tilgen W, Dzarlieva RT, Breitkreutz D, Haag D, Riehl RK, Bohnert A, Fusenig NE (1982) Phenotypic and genotypic characteristics of a cell line from a squamous cell carcinoma of human skin. (springer.com)
  • phenotypic
  • Further, cell lines can change genetically over multiple passages, leading to phenotypic differences among isolates and potentially different experimental results depending on when and with what strain isolate an experiment is conducted. (wikipedia.org)
  • receptor alpha
  • A variant of the AKR thymona BW5147 has been isolated which can no longer express functional T cell receptor alpha- and beta- chains. (nationaljewish.org)
  • Glial cell line-derived neurotrophic factor has been shown to interact with GFRA2 and GDNF family receptor alpha 1. (wikipedia.org)
  • Isolation
  • There are several methods for generating immortalized cell lines: Isolation from a naturally occurring cancer. (wikipedia.org)
  • A Javan banteng calf was successfully cloned from frozen cells using a cow as a surrogate, delivered via c-section April 1, 2003 then hand raised at the San Diego Wild Animal Parks Infant Isolation Unit. (wikipedia.org)
  • Cellular
  • An immortalized cell line is a population of cells from a multicellular organism which would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division. (wikipedia.org)
  • proteins
  • PC12 cell line use has given much information to the function of proteins underlying vesicle fusion. (wikipedia.org)
  • The cells are used for a wide variety of purposes, from testing toxicity of compounds or drugs to production of eukaryotic proteins. (wikipedia.org)
  • Artificial expression of key proteins required for immortality, for example telomerase which prevents degradation of chromosome ends during DNA replication in eukaryotes Hybridoma technology, specifically used for the generation of immortalized antibody-producing B cell lines, where an antibody-producing B cell is fused with a myeloma (B cell cancer) cell. (wikipedia.org)
  • lineage
  • U937 cells are of the myeloid lineage and so secrete a large number of cytokines and chemokines either constitutively (e.g. (wikipedia.org)
  • biology
  • LGC Promochem: Cell Biology Collection. (wikipedia.org)
  • Immortal cell lines are a very important tool for research into the biochemistry and cell biology of multicellular organisms. (wikipedia.org)
  • This simplifies analysis of the biology of cells which may otherwise have a limited lifetime. (wikipedia.org)
  • This can alter the biology of the cell and must be taken into consideration in any analysis. (wikipedia.org)
  • The cell line, isolated by Leonard Hayflick and Paul Moorhead in the 1960s, has been used extensively in scientific research, with applications ranging from developing important theories in molecular biology to the production of many types of vaccines. (wikipedia.org)
  • secrete
  • Infected WI-38 cells secrete the virus, and can be cultured in large volumes suitable for commercial production. (wikipedia.org)