• We have solved the X-ray crystal structure of the RNA chaperone protein Hfq from the alpha-proteobacterium Caulobacter crescentus to 2.15-Å resolution, resolving the conserved core of the protein and the entire C-terminal domain (CTD). (johnshopkins.edu)
  • As a model system, they exploited the gram-negative, alpha-proteobacterium Caulobacter crescentus, which is widely present in fresh-water lakes and streams. (asbmb.org)
  • Caulobacter crescentus is a Gram-negative, oligotrophic bacterium widely distributed in fresh water lakes and streams. (wikipedia.org)
  • Caulobacter crescentus is an aquatic free-living Gram-negative bacterium whose cell cycle depends on cell differentiation. (usp.br)
  • The dimorphic bacterium Caulobacter crescentus is a model organism for studying the bacterial cell cycle. (csun.edu)
  • Unexpectedly, they come from studying the bacterium Caulobacter crescentus , long used as a model organism for understanding cell differentiation and the cell cycle, but not known to produce GTAs. (asmblog.org)
  • In order to best accomplish our goals, we use the genetically tractable bacterium Caulobacter crescentus whose cell cycle can be easily synchronized to enable fine temporal resolution of each cell cycle event. (illinois.edu)
  • In the laboratory, researchers distinguish between C. crescentus strain CB15 (the strain originally isolated from a freshwater lake) and NA1000 (the primary experimental strain). (wikipedia.org)
  • The Caulobacter CB15 genome has 4,016,942 base pairs in a single circular chromosome encoding 3,767 genes. (wikipedia.org)
  • In 2010, the Caulobacter NA1000 strain was sequenced and all differences with the CB15 "wild type" strain were identified. (wikipedia.org)
  • CIL:40019, Caulobacter crescentus CB15. (ucsd.edu)
  • Lucy Shapiro, Harley McAdams (2012) CIL:40019, Caulobacter crescentus CB15. (ucsd.edu)
  • Due to this capacity to be physically synchronized, strain NA1000 has become the predominant experimental Caulobacter strain throughout the world. (wikipedia.org)
  • Since previous interspecies comparisons of Caulobacter genomes have revealed extensive genome rearrangements, we decided to compare the nucleotide sequences of four C. crescentus genomes, NA1000, CB1, CB2, and CB13. (pacb.com)
  • As a card-carrying cell biologist, Goley was next drawn to the fledgling field of bacterial cell biology and brought her expertise in eukaryotic cytoskeletal biochemistry to studying the cytoskeleton of Caulobacter crescentus as a postdoctoral researcher with Lucy Shapiro at Stanford University. (rupress.org)
  • So in December 1996, she joined the lab of Lucy Shapiro at Stanford University, where she spent four productive years exploring how Caulobacter coordinates its developmental program with cell-cycle progression. (the-scientist.com)
  • C. crescentus derives its name from its crescent shape, which is caused by the protein crescentin. (wikipedia.org)
  • The regular surface protein structure (S-layer) of Caulobacter crescentus was analyzed by electron microscopy and three-dimensional image reconstruction to a resolution of 2 nm. (mpg.de)
  • Negative-stain and thin-section electron microscopy examination of colonies of C. crescentus strains with a mutation in a surface molecule involved in the attachment of the S-layer showed that shed RsaA protein organized into large sheets. (mpg.de)
  • In addition, the expression of these fusions are no longer cell-cycle regulated, as well as GroEL synthesis in a strain which does not have the HrcA protein, the putative repressor that binds CIRCE, indicating that the CIRCE-HrcA system are involved in cell cycle regulation of groESL in C. crescentus. (usp.br)
  • Their research group focused on understanding the progression of the cell division cycle throughout successive developmental events and how multiple histidine protein kinases regulate the signal transduction pathways of C. crescentus in cell division. (asbmb.org)
  • Caulobacter is famous for its dimorphic life cycle: it has two primary cell types, a motile form called the swarmer and a sessile form called the stalked cell, and it produces one of each through an obligate asymmetric cell division. (rupress.org)
  • Frontispiece: Transmission electron micrograph of filtered supernatant showing spherical particles from C. crescentus ΔrogA cells in stationary phase. (asmblog.org)
  • Characterization of the Proteins Associated with Caulobacter crescentus Bacteriophage CbK Particles. (cdc.gov)
  • Yet, Caulobacter has the swarmer cell stage that results in slower population growth. (wikipedia.org)
  • Swarmer cells from wild-type C. crescentus were isolated and allowed to proceed synchronously through their 150-min cell cycle (Fig. 1A). (harvard.edu)
  • C. crescentus cell division is assymetric, and results in a sessile stalked cell and a motile swarmer cell. (science20.com)
  • Since many of the proteins involved in regulating the cell cycle of C. crescentus are conserved among many genera of a-proteobacteria, the proposed mechanism may be applicable to other species of importance in agriculture and medicine. (ebi.ac.uk)
  • The Caulobacter cell cycle regulatory system controls many modular subsystems that organize the progression of cell growth and reproduction. (wikipedia.org)
  • An essential regulatory function of the DnaK chaperone dictates the decision between proliferation and maintenance in Caulobacter crescentus. (scilifelab.se)
  • Here, we demonstrate that DnaK is essential in the α-proteobacterium Caulobacter crescentus due to its regulatory function in gene expression. (scilifelab.se)
  • Genome Comparisons of Wild Isolates of Caulobacter crescentus Reveal Rates of Inversion and Horizontal Gene Transfer. (pacb.com)
  • Transposon mutagenesis paired with deep sequencing of Caulobacter crescentus under uranium stress reveals genes essential for detoxification and stress tolerance. (csun.edu)
  • We conclude that Caulobacter has co-opted the DnaK chaperone system as an essential regulator of gene expression under conditions when its folding activity is dispensable. (scilifelab.se)
  • Regarding antibiotics, we have isolated mutants of Caulobacter crescentus that exhibit multidrug resistance and display developmental problems. (illinois.edu)
  • Thus, we estimate that inversions occur at a rate of one per 10 to 12 million generations in Caulobacter genomes. (pacb.com)
  • evolved Caulobacter crescentus for 2000 generations under conditions where selection was strong early in life, but weak late in life. (science20.com)
  • From published experimental evidence, we propose a molecular mechanism for control of the cell division cycle in Caulobacter crescentus. (ebi.ac.uk)
  • Caulobacter cultures (strain 3724) were prefixed with 1% acrolein and 2.5% glutaradehyde, followed by high pressure freezing, and freeze substitution using a cocktail of 2% Osmium tetroxide, 0.5% uranyl acetate, and embedding in Durcupan. (ucsd.edu)
  • Detailed study of the molecular development of these cells as they progress through the cell cycle has enabled researchers to understand Caulobacter cell cycle regulation in great detail. (wikipedia.org)
  • During my thesis, I characterized at the molecular level the involvement of PTS Ntr in the control of Caulobacter crescentus asymmetric cell cycle. (unamur.be)
  • Her pioneering studies on the molecular mechanisms underlying cell shape and cell polarity in Caulobacter crescentus , says Errington, "have helped change the way people think about bacteria. (the-scientist.com)
  • Caulobacter daughter cells have two very different forms. (wikipedia.org)
  • This work shows that GTAs produced by C. crescentus help cells overcome the lethal effect of DNA damage. (asmblog.org)
  • When Caulobacter divides, it does so asymmetrically, producing daughter cells that have different fates. (the-scientist.com)
  • Current research examines the general principles and spatiotemporal mechanisms by which bacterial cells replicate, using Caulobacter crescentus and Escherichia coli as models. (stanford.edu)
  • This work might lay the foundation for further studies on the intertwining of phosphate, nitrogen and potentially carbon metabolisms and their consequences on Caulobacter cell cycle. (unamur.be)
  • The model accounts for important details of the physiology, biochemistry, and genetics of cell cycle control in stalked C. crescentus cell. (ebi.ac.uk)
  • 2012. The Caulobacter crescentus ctrA P1 promoter is essential for the coordination of cell cycle events that prevent the overinitiation of DNA replication. (csun.edu)
  • In Shapiro's laboratory, Goley used Caulobacter as a model system to investigate the role of the conserved tubulin-like GTPase, FtsZ, in orchestrating bacterial division ( 3 , 4 ). (rupress.org)
  • Shapiro gave Goley the support and intellectual freedom to pursue whatever questions inspired her "as long as it was in Caulobacter ! (rupress.org)
  • p)ppGpp modulates cell size and the initiation of DNA replication in Caulobacter crescentus in response to a block in lipid biosynthesis. (csun.edu)
  • The Caulobacter stalked cell stage provides a fitness advantage by anchoring the cell to surfaces to form biofilms and or to exploit nutrient sources. (wikipedia.org)
  • A 512 by 512 image from the slice by slice reconstruction of a caulobacter crescentus specimen. (ucsd.edu)
  • construct simple models to show why organisms might evolve aging, and test these models using age-specific performance data of C. crescentus to test the assumptions of the models. (science20.com)