• The secondary objective was to assess the effect of anti-OX40 on lymphocyte subsets in the tumor and blood. (bepress.com)
  • The relative number of different lymphocyte subsets remained constant throughout the experiment. (usda.gov)
  • CD4+ T-cells and other lymphocyte subsets in persons infected with human immunodeficiency virus (HIV). (cdc.gov)
  • The guidelines describe single-platform technology (SPT), a process in which absolute counts of lymphocyte subsets are measured from a single tube by a single instrument. (cdc.gov)
  • With CD45 gating, the relative numbers of beads and lymphocyte subsets are enumerated, and their absolute numbers and percentage values are calculated. (cdc.gov)
  • The level of The infants with PEM were enrolled in T-lymphocyte subsets in peripheral blood the study after fulfilling a set of inclusion provide information about the development criteria. (who.int)
  • The aim of our research was to determine how immunotherapy changes the proportion of lymphocyte subsets in dog peripheral blood and the levels of cytokines secreted by these cells during therapy. (mdpi.com)
  • It is common to evaluate lymphocytes subsets in virus infection, immunodeficiency diseases and cancer. (researchsquare.com)
  • This study retrospectively analyzed serum lymphocyte subsets and survival outcomes in intracranial germ cell tumors (iGCTs) patients. (researchsquare.com)
  • Serum from145 iGCTs patients were collected and lymphocyte subsets were analyzed by flow cytometry. (researchsquare.com)
  • Dynamic monitoring of serum lymphocyte subsets can be used as an auxiliary indicator for prognosis judgment. (researchsquare.com)
  • HIV-1 may infect thymic precursor cells destined to become CD4 and CD8 lymphocytes and contribute to the numerical decline in both subsets on disease progression. (ox.ac.uk)
  • To investigate the relationship between CD8 activation and infection by HIV-1 in vivo, activated subsets of CD8 lymphocytes in peripheral blood mononuclear cells (PBMCs) of HIV-seropositive individuals were investigated for CD4 expression and HIV infection. (ox.ac.uk)
  • Macrophages and T lymphocytes demonstrated a marked expression of HLA-DR antigen. (wikipedia.org)
  • Mycobacterium bovis, the causative agent of bovine tuberculosis is known to persist within granulomas, distinct lesions represented by a caseonecrotic core surrounded by epithelioid macrophages, T-lymphocytes, B-lymphocytes, Langhans-type multinucleated giant cells and fibroblasts. (usda.gov)
  • iNOS positive cells consisted of epithelioid macrophages and multinucleated giant cells surrounding central areas of caseous necrosis. (usda.gov)
  • Early in the disease process, the epidermis becomes infiltrated with CD8 T lymphocytes and macrophages, whereas the dermis displays a slight influx of CD4 lymphocytes. (medscape.com)
  • Starting with colorectal cancer, the researchers relied on multiplex immunohistochemistry to profile immune cells spanning 15 classes, coming up with a tumor microenvironment "signature of immune activation" (SIA) that took anti-tumor CD8-positive lymphocyte and tumor-associated macrophages into account. (genomeweb.com)
  • Scattered T gamma-delta and B lymphocytes, macrophages and NK cells were observed among the cells of the dermal infiltrate of vulvar condylomata. (medicaljournals.se)
  • Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. (thermofisher.com)
  • The specific targeting of responding CD8 lymphocytes may provide a functional explanation for the previously observed impairment of cytotoxic T-lymphocyte (CTL) function disproportionate to their numerical decline in AIDS and for the deletion of specific clones of CTLs responding to HIV antigens. (ox.ac.uk)
  • CD8 responses were assessed in vitro for cytotoxicity and in vivo tumor therapy. (bmj.com)
  • At week 4, vaccine antigen-specific T cell responses were detected in 28 (93.3%) and 18 (60%) of 30 vaccine recipients for CD4(+) and CD8(+) T cells, respectively, by intracellular cytokine staining assay and in 22 (73%) of 30 vaccine recipients by enzyme-linked immunospot assay. (emmes.com)
  • A single injection induced HIV-1 antigen-specific CD4(+) T cell, CD8(+) T cell, and antibody responses in the majority of vaccine recipients. (emmes.com)
  • MHC-I and MHC-II proteins are key components of the antigen presentation machinery responsible for neoantigen presentation to CD8 + and CD4 + T lymphocytes, respectively. (portlandpress.com)
  • Immunohistochemically, the vast majority of the infiltrating cells stained positive for CD3 (Figure 6 ) and over half of them were positive for CD2 and T-cell intracellular antigen 1 (TIA1). (upmc.edu)
  • Activated CD8 lymphocytes were identified by expression of CD69, CD71, and the human leukocyte antigen (HLA) class II, the beta-chain of CD8, and the RO isoform of CD45. (ox.ac.uk)
  • Those condylomata with moderate to severe mononuclear infiltrate showed leucocyte function antigen 1 positive T cells forming small clusters in the lower epithelial half around the ICAM-1 positive keratinocytes. (medicaljournals.se)
  • To determine the immunologic characteristics of tumor infiltrating lymphocytes (TILs) in comparison with the corresponding peripheral blood lymphocytes (PBLs) of patients with ovarian cancer in order to detect specific antitumor-reactive-immunocompetent cells. (nih.gov)
  • Peripheral blood lymphocytes had a higher proliferative activity and cytotoxicity against natural killer-sensitive tumor cells than the corresponding TILs. (nih.gov)
  • Peripheral blood lymphocytes showed more potentially valuable behavioral characteristics than TILs. (nih.gov)
  • Peripheral Blood Mononuclear Cells (PBMC) are labeled with BD IMag™ Anti-Human CD8 Magnetic Particles - DM according to the Magnetic Labeling Protocol. (bdbiosciences.com)
  • Flow cytometric analysis of CD16 on human peripheral blood lymphocytes. (bdbiosciences.com)
  • Foremost, N-809 enhances intratumoral lymphocyte numbers by increasing trafficking via altered chemokine levels in the TME and chemokine receptor expression on CD8 + T cells and NK cells. (bmj.com)
  • Adoptive cell therapy using tumor-infiltrating lymphocytes (TILs) has shown activity in melanoma, but has not been previously evaluated in metastatic non-small cell lung cancer. (nature.com)
  • Comparison of tumor biopsies before and after treatment reveals an increase of activated, conventional CD4+ tumor-infiltrating lymphocytes (TIL) in most patients and higher clonality by TCRβ sequencing. (bepress.com)
  • Tumor infiltrating lymphocytes and PBLs were phenotyped by their surface markers, cytokine pattern, proliferation rate, and cytotoxic ability. (nih.gov)
  • Previous results from two proficiency panels of intracellular cytokine staining (ICS) from the Cancer Immunotherapy Consortium and panels from the National Institute of Allergy and Infectious Disease and the Association for Cancer Immunotherapy highlight the variability across laboratories in reported % CD8+ or % CD4+ cytokine-positive cells. (nih.gov)
  • As shown by multiplex tissue immunofluorescence and intravital microscopy, combined immunotherapy treatments enhance T cell infiltration and the intratumoral performance of T lymphocytes. (unav.edu)
  • CD4 helper T-cells which alert the immune the studied infants were from low socio- system to an attack by a pathogen and the economic status families according to the CD8 suppressor T-cells which destroy cells classification of Park and Park [11]. (who.int)
  • CD3 is a generic T-cell receptor and will identify all T-cells while CD8 is a marker for cytotoxic T-cells and suppressor T cells [ 10 ]. (biomedcentral.com)
  • HIV antibodies were undetectable, and CD4/CD8 lymphocyte counts were within reference ranges. (cdc.gov)
  • T-lymphocyte counts, with special emphasis and stunted children has not changed dra- on the effect of nutritional rehabilitation. (who.int)
  • Stimulated recovery of leukocyte, lymphocytes, and NK cells counts were observed in mice pre-treated with Vitamin c . (biomedgrid.com)
  • Notably, vaccination with DREP-GP and DREP-VP40, which produces both GP and VP40 antigens, induced a significantly higher level of anti-GP IgG2a antibody and increased IFN-γ secreting CD8 + T-cell responses relative to vaccination with DREP-GP or DREP-VP40 vector alone. (frontiersin.org)
  • Recent studies suggested that strategies to reduce immunosuppressive cells, promote systemic T-cell responses and lymphocyte trafficking to the tumor microenvironment (TME) may improve efficacy. (bmj.com)
  • Results Homocitrullinated peptides from aldolase and cytokeratin were identified, that stimulated CD8-mediated responses in vivo. (bmj.com)
  • Modified peptides showed enhanced binding to HLA-A2 compared with the native sequences and immunization of HLA-A2 transgenic mice generated high avidity modification specific CD8 responses that killed peptide expressing target cells. (bmj.com)
  • Thus, Hcit-specific CD8 T-cell responses have potential in the development of future anticancer therapy. (bmj.com)
  • This study provides the first evidence that homocitrullinated proteins in tumors can also be targets for CD8 T-cell responses. (bmj.com)
  • Implement of comprehensive treatments led to positive responses. (researchsquare.com)
  • The demonstration of antibodies, mixed T-helper (Th) type 1/2 responses diisocyanate occupational asthma in a murine model after sub- and the involvement of CD8 T lymphocytes in chronic inhalation exposure at relevant exposure levels should diisocyanate OA. (cdc.gov)
  • Compared with N-803+αPD-L1, N-809 enhanced natural killer (NK) and CD8 + T-cell activation and function in the dLN and TME, relating to increased gene expression associated with interferon and cytokine signaling, lymphoid compartment, costimulation and cytotoxicity. (bmj.com)
  • The focus of Dr. Berg's work is on the signaling proteins and pathways regulating T lymphocyte development, differentiation, activation, and migration, with a strong emphasis on T-cell receptor (TCR) signaling. (cuanschutz.edu)
  • It neutralizes pathogenic cytokines such as tumor necrosis factor and interleukin-1 as well as superantigens that prevent activation of classes of T lymphocytes. (nursingcenter.com)
  • Differentiating between low-grade lymphoma and reactive lymphocytes is often difficult by morphology alone as reactive lymphoid cells may acquire activation morphology from being exposed to different cytokines within the body fluid. (cytojournal.com)
  • CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells. (thermofisher.com)
  • Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. (thermofisher.com)
  • Importantly, in vivo the homocitrullinated aldolase specific response was associated with efficient CD8 dependent antitumor therapy of the aggressive murine B16 tumor model indicating that this epitope is naturally presented in the tumor. (bmj.com)
  • In addition, the feasibility of imaging of DAbR1-positive T cells in vivo after intravenous injection of 86 Y/ 177 Lu-AABD was studied and radiation doses determined. (snmjournals.org)
  • Imaging studies showed 86 Y-AABD was retained by DAbR1-positive T cells while it continuously cleared from normal tissues, allowing for in vivo tracking of intravenously administered CAR T cells. (snmjournals.org)
  • Activated peripheral CD8 lymphocytes express CD4 in vivo and are targets for infection by human immunodeficiency virus type 1. (ox.ac.uk)
  • There is increasing evidence that CD8 lymphocytes may represent targets for infection by human immunodeficiency virus type 1 (HIV-1) in vivo whose destruction may contribute to the loss of immune function underlying AIDS. (ox.ac.uk)
  • Combined, these findings provide evidence that antigenic stimulation of CD8 lymphocytes in vivo induces CD4 expression that renders them susceptible to HIV infection and destruction. (ox.ac.uk)
  • Estos linfocitos citotóxicos pueden generarse in vitro en cultivos mixtos de linfocitos (MLC, siglas en inglés), in vivo durante una reacción de injerto contra huésped (GVH, siglas en inglés), o después de la inmunización con un aloinjerto, célula tumoral o célula diana modificada químicamente o transformada por virus. (bvsalud.org)
  • These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. (bvsalud.org)
  • This led to high variability in the reported percentage of cytokine-positive cells and consequently in response detection in Phase I. However, variability was dramatically reduced when all laboratories used the same gating strategy (Phase II). (nih.gov)
  • Proximity of the cytokine gate to the negative population most impacted true-positive and false-positive response detection. (nih.gov)
  • Recommendations are provided for the (1) placement of the cytokine-positive gate, (2) identification of CD4+ CD8+ double-positive T cells, (3) placement of lymphocyte gate, (4) inclusion of dim cells, (5) gate uniformity, and 6) proper adjustment of the biexponential scaling. (nih.gov)
  • Median survival was 45.81 months and patients of increased Tc cells, activated T helper cells(CD4 + CD25 + T cells or activated Th cells), activated Tc cells and normal CD4 + /CD8 + ratio presented encouraging outcomes (p = 0.007;0.016;0.028;0.016 respectively). (researchsquare.com)
  • The researchers discovered that, among the 22 patients who survived for a shorter period than the average time (a median survival of 313 days), immune cells called IL-10-positive myelomonocytes tended to be located close to a cluster of granzyme B-positive CD8-positive T cells (or cytotoxic T lymphocytes). (ascopost.com)
  • Among the 23 patients who survived longer than average (a median survival of 832 days), the same myelomonocytes were more grouped near a different type of T cell, known as PD-1-positive CD4-positive T cells (or activated helper T cells). (ascopost.com)
  • X-tile software (Yale School of Medicine, CT, USA) was used to stratify patients into 'high' and 'low' for each of the lymphocytes stained and their association with survival. (biomedcentral.com)
  • The presence of intratumoural CD3 positive cells was associated with improved survival (p = 0.028), and intratumoural and stromal CD3 in combination also correlated with improved survival (p = 0.043). (biomedcentral.com)
  • When CD20 positive lymphocyte levels were high, survival improved (p = 0.029) and similar results were seen for CD20 in combination with intratumoural CD3 (p = 0.001) and stromal CD8 (p = 0.013). (biomedcentral.com)
  • Based on the studies of survival, behavior of hematograms, and numbers of lymphocytes, whole body following irradiation, it was demonstrated that Vitamin c was an effective radioprotector. (biomedgrid.com)
  • Description: The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). (thermofisher.com)
  • Ongoing research investigates mechanisms invoked by cancer cells to abrogate MHC-I expression and attenuate anti-tumour CD8 + cytotoxic T cell response. (portlandpress.com)
  • Tumour-associated lymphocytes (TALs) have been linked with good prognosis in several solid tumours. (biomedcentral.com)
  • The number of lymphocytes within specific tumour compartments (i.e. stromal and intratumoural) was quantified. (biomedcentral.com)
  • CD3 and CD8 positive lymphocytes were associated with grade of tumour differentiation. (biomedcentral.com)
  • Occasional lymphocytes expressed CD4, CD5, CD8 and CD30, while staining for the B-cell marker CD20 and for CD56, activin receptor-like kinase 1 (Alk1) and T-cell receptor alpha/beta (TCR alpha/beta) was consistently negative. (upmc.edu)
  • This study aimed to evaluate the prognostic significance of CD3, CD8 and CD20 positive lymphocytes in pancreatic ductal adenocarcinoma. (biomedcentral.com)
  • After histological re-evaluation of the tumours of 81 patients who underwent surgical resection for exclusively pancreatic ductal adenocarcinoma, tissue micro-arrays (TMA) were constructed and immunohistochemistry was performed for CD3, CD8 and CD20. (biomedcentral.com)
  • This study evaluated the prognostic significance of CD3, CD8 and CD20 positive lymphocytes in pancreatic ductal adenocarcinoma. (biomedcentral.com)
  • We developed a CD8 + T-cell-based vaccine platform based on intramuscular (i.m.) injection of a DNA vector coding for antigens of interest fused at the C-terminus of a biologically inactive Human Immunodeficiency Virus (HIV)-Type 1 Nef protein (Nef mut ) having an unusually high efficiency of incorporation into EVs. (nature.com)
  • Methods: Children with a positive (OFC + , n = 16) or a negative (OFC − , n = 10) OFC-outcome were included (controls, n = 7). (sdu.dk)
  • To assure the accuracy and reliability of CD4+ T-lymphocyte test results obtained within individual laboratories and to assure the comparability of results between laboratories, standard methods for performing the test, as well as guidelines for quality control and quality assurance, are desirable. (cdc.gov)
  • CD4+ T-lymphocyte levels are also used as prognostic indicators in patients with human immunodeficiency virus (HIV) disease (8). (cdc.gov)
  • Whole EDTA anti-coagulated blood stored at -70°C from the 31 HIV antibody-positive participants from NHANES 1999-2004 and 34 age-matched controls were used. (cdc.gov)
  • To fill the gap, we investigated the immunity generated in the lungs by N-engineered EVs in terms of induction of N-specific effectors and resident memory CD8 + T lymphocytes before and after virus challenge carried out three weeks and three months after boosting. (nature.com)
  • They believe that cytotoxic T lymphocytes are apoptotic effectors as well as target cells and that histiocytes could possibly enhance apoptosis in Kikuchi disease. (medscape.com)
  • The origin of the reactive cells in such patients are either thymic release of T-cells that expand at the periphery or transplacentally transfers of maternal T lymphocytes. (hindawi.com)
  • All enrolled thymic lymphocytes is widely accepted as infants were breastfed in addition to receiv- an indicator of the depression of thymus- ing some traditional foods, according to dependent immune competence associated their age. (who.int)
  • The higher number of TME CD8 + T cells was attributed to enhanced infiltration, not in situ expansion. (bmj.com)
  • The antiviral effect appeared similarly strong when the viral challenge was carried out 3 months after boosting, and associated with the persistence of N-specific CD8 + T-resident memory lymphocytes. (nature.com)
  • Positive associations between AR activity and ERVs/antiviral pathways were evident in patient transcriptomic data, supporting the clinical relevance of our findings. (cam.ac.uk)
  • Many other studies have used a similar strategy and in a recent meta-analysis of the role of TALs [ 14 ], it was concluded that CD3 and CD8 were associated with good prognosis. (biomedcentral.com)
  • Moreover, cIAPs are required for CD137 signaling toward the NF-?B and MAPK pathways and for costimulation of human and mouse T lymphocytes. (unav.edu)
  • Almost half of the cells were positive for the activated T-cell marker granzyme B, and some for CD7. (upmc.edu)
  • While the autoreactive cells displayed significantly reduced Tregs numbers, the alloreactive transplacentally acquired maternal lymphocytes had high functional Tregs. (hindawi.com)
  • Enumeration of CD4+ lymphocytes in HIV-positive participants and age-matched controls was performed on cryopreserved whole blood using the method reported by Fiebig et. (cdc.gov)
  • This research suggests that post-translational modification of proteins provide efficient targets in tumors for both CD4 and CD8 T-cell-mediated therapies. (bmj.com)
  • For flow cytometric analysis, fresh PBMC (left panel), the negative fraction (middle panel) and the positive fraction (right panel) were stained with PE Mouse Anti-Human CD8 (Cat. (bdbiosciences.com)
  • Specifically, from one case of Kikuchi disease, they reported that the in situ hybridization and immunohistochemistry double-staining assay results revealed that parvovirus B19-infected cells were mainly lymphocytes and a small number of histiocytes. (medscape.com)
  • A low ratio of CD4+ (helper) any chromosomal or hereditary disorder lymphocytes relative to CD8+ (suppressor) that caused the malnutrition. (who.int)
  • In patients who survived longer than the average, however, researchers proposed that the activated helper T cells switched off the myelomonocytes, which, in turn, let the cytotoxic T lymphocytes more effectively fight the cancer. (ascopost.com)
  • HIV infection is characterized by a decrease and, eventually, a depletion of CD4+ T-lymphocytes (helper T cells). (cdc.gov)
  • Using immunophenotyping, HIV-positive blood samples and age-matched controls were tested for the proportion of lymphocytes that are T cells, B cells, natural killer (NK) cells, CD4+ T cells (helper T cells), and CD8+ T cells (suppressor/inducer T cells). (cdc.gov)
  • T lymphocytes with a mean CD4/CD8 ratio of 0.25 and a mean density of 267 +/- 59 cells/mm2 of epithelial section were the main cellular infiltrate in vulvar papillomavirus infection. (medicaljournals.se)
  • Single-platform technology (SPT) is designed to enable de- system and managing the health care of persons infected with terminations of both absolute and percentage lymphocyte sub- human immunodeficiency virus (HIV) ( 1-4 ). (cdc.gov)
  • We suggest that a high-tolerance capacity of the alloreactive transplacentally acquired maternal lymphocytes represents a toleration advantage, yet still associated with severe immunodeficiency. (hindawi.com)
  • This document has been developed by CDC to give guidance to laboratories performing lymphocyte immunophenotyping assays in human immunodeficiency virus-infected persons. (cdc.gov)
  • The pathogenesis of acquired immunodeficiency syndrome (AIDS) is largely attributable to the decrease in T-lymphocytes bearing the CD4 receptor (CD4+) (1-5). (cdc.gov)
  • However, in most cases there are specific features that may aid in differentiating small reactive from non-reactive lymphocytes including the round shape of the nucleus, the absence of visible nucleoli and the presence of fine clumped chromatin. (cytojournal.com)
  • However, nothing is known about the ability of the N-specific CD8 + T cell immunity in controlling viral replication in the lungs, a major pathogenic signature of severe disease in humans. (nature.com)
  • Increased TME NK and CD8 + T-cell numbers correlated with augmented chemokine ligands and receptors. (bmj.com)
  • W]e have identified an immune cell signature combining CD8 cytotoxic T-cells and a distinct TAM subset reflecting anti- and pro-tumorigenic immune reactions," they report. (genomeweb.com)
  • The aim of this study was to investigate whether DAbR1 can be expressed on lymphocytes and used as a reporter gene as well as a suicide gene for therapy of immune-related adverse effects. (snmjournals.org)
  • and the percentage of lymphocytes that are CD4+ T-cells. (cdc.gov)
  • The process of measuring the percentage of CD4+ T-lymphocytes in the whole blood sample is referred to as ``immunophenotyping by flow cytometry'' (9-14). (cdc.gov)
  • A distinctive feature of SCID patients, which sometimes can clinically resemble Omenn, [ 6 ] is the presence of alloreactive cells originated from transplacentally maternal T lymphocytes. (hindawi.com)
  • Blood from patients treated with IVIG is reported to have increased suppressor CD8-positive T-cells. (nursingcenter.com)
  • Cytotoxic T lymphocytes produced a toxin capable of killing cancer cells, but the researchers hypothesized that the nearby myelomonocytes blocked that ability in patients who survived shorter than the average. (ascopost.com)
  • Serial monitoring of CD4 T cell count in HIV-positive patients. (bumrungrad.com)
  • The progressive loss of CD4 T-lymphocytes in patients infected with HIV is associated with increased infections and complications. (bumrungrad.com)
  • The Public Health Service has recommended that all HIV-positive patients be tested every 3 to 6 months for the level of CD4 T-lymphocytes. (bumrungrad.com)
  • After the positive fraction is washed, the small size of the magnetic particles allows the positive fraction to be evaluated in downstream applications such as flow cytometry. (bdbiosciences.com)