- Spin down briefly and dilute with 2xSDS Sample Buffer provided (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT). (lsbio.com)
- The slurry was washed four times with wash buffer containing protease inhibitors and the biotinylated proteins were solubilized in 400 μl of 4× SDS-PAGE sample buffer (50 mm Tris pH 6.8 2 SDS 10 glycerol 0.01% bromphenol blue and 50 mm DTT) for 60 BIO-32546 min at room temperature with rotation. (healthcarecoremeasures.com)
- The immune complexes were collected on protein A-agarose during 1 h of incubation at 4 °C. The beads were washed three times with washing buffer (50 mm Tris pH 7.5 1 Nonidet P-40 150 mm NaCl 2 mm EGTA) and boiled for 5 min in SDS-PAGE sample buffer (50 mm Tris-HCl pH 6.8 2 SDS 2 β-mercaptoethanol 10 glycerol 0.005% bromphenol blue). (healthyguide.info)
- Loading Buffer II is a 6x dying solution for gel electrophoresis with 0.1 % Bromphenol blue sodium salt, 0.1 % Xylene cyanol FF and 30 % Glycerin. (bioron.net)
- In 1 % agarose gels, bromphenol blue migrates with 300 bp linear double-stranded DNA fragment, while xylene cyanol FF migrates at approximately the same rate as linear doublestranded DNA fragments of 4 kb length. (bioron.net)
- 138 mm unbuffered Tris-HCl, 73% (v/v) formamide, 2 mm Tris borate, 86 mm EDTA, pH 8, with xylene cyanol and bromphenol blue) was added, and the samples were heated at 95 °C for an additional 5 min. (ncsu.edu)
- the rate at which it migrates varies according to gel density and buffer composition, but in a typical 1% agarose gel in a 1X TAE buffer or TBE buffer, bromophenol blue migrates at the same rate as a DNA fragment of about 300 base pairs, in 2% agarose as 150 bp. (wikipedia.org)
- Bromophenol blue is structurally related to phenolphthalein (a popular indicator). (wikipedia.org)
- In solution at pH 3.6 (in the middle of the transition range of this pH indicator) obtained by dissolution in water without any pH adjustment, bromophenol blue has a characteristic green red colour, where the apparent colour shifts depending on the concentration and/or path length through which the solution is observed. (wikipedia.org)
- At specific pH values soaps mixed with bromphenol, generate a specific blue colour. (cdrfoodlab.com)
- At low pH, the dye absorbs ultraviolet and blue light most strongly and appears yellow in solution. (wikipedia.org)
- This procedure for routine quantification of albumin in urine is based on the dye-binding properties of albumin with bromphenol blue. (lookformedical.com)
- At neutral pH, the dye absorbs red light most strongly and transmits blue light. (wikipedia.org)
- Membranes had been visualized using the Traditional western Light Chemiluminescence Reagent (PerkinElmer Existence Sciences) and Kodak X-OMAT Blue XB-1 film (PerkinElmer Existence Sciences) was subjected and developed utilizing a Kodak X-OMAT 1000A processor chip. (healthcarecoremeasures.com)
- When exposed to blue light (450-500 nm), Auramine O becomes excited and emits light in the range of around 500 nm to 700 nm (green-yellow-red). (macsenlab.com)
- Bromophenol blue is structurally related to phenolphthalein (a popular indicator). (wikipedia.org)
- SiSpecifications Alkalinity (as CaCO3) Type Titration Range 0-100mg/L (ppm)0-300 mg/L (ppm) Smallest increment 1mg/L (ppm)3mg/L (ppm) Method phenolphthalein/bromphenol blue Number of tes. (indonetwork.co.id)
- the rate at which it migrates varies according to gel density and buffer composition, but in a typical 1% agarose gel in a 1X TAE buffer or TBE buffer, bromophenol blue migrates at the same rate as a DNA fragment of about 300 base pairs, in 2% agarose as 150 bp. (wikipedia.org)
- Bromophenol blue is also used as a dye. (wikipedia.org)
- In solution at pH 3.6 (in the middle of the transition range of this pH indicator) obtained by dissolution in water without any pH adjustment, bromophenol blue has a characteristic green red colour, where the apparent colour shifts depending on the concentration and/or path length through which the solution is observed. (wikipedia.org)
- Bromophenol blue is the substance with the highest known value of Kreft's dichromaticity index. (wikipedia.org)
- Bromophenol blue (115-39-9) Chemical Names and Synonyms. (wikipedia.org)
- Product Specification : Bromophenol Blue" (PDF). (wikipedia.org)
- To describe visualization of the vitreous and the posterior hyaloid membrane using bromophenol blue during vitrectomy for macular hole and retinal detachment. (nih.gov)
- Bromophenol blue provided sufficient staining of the attached posterior hyaloid membrane and vitreous remnants in the periphery. (nih.gov)
- Delineation of the vitreous and the posterior hyaloid using bromophenol blue staining greatly facilitates vitreoretinal procedures. (nih.gov)
- Bromophenol blue appeared to be a very helpful and safe tool to visualize the posterior hyaloid membrane in macular hole surgery and assured its complete separation from the retinal surface. (nih.gov)
- Therefore, bromophenol blue appears as a very good alternative to triamcinolone, which has been used for this purpose, because the dye has no pharmacological properties and no side effects are likely to occur such as cataract formation and increase in intraocular pressure. (nih.gov)
- The method is based on the interaction of the drug base with bromophenol blue to give a stable ion-pair complex. (nih.gov)
- Extractive spectrophotometric methods for determination of diltiazem HCl in pharmaceutical formulations using bromothymol blue, bromophenol blue and bromocresol green. (nih.gov)
- Therefore, loading buffer containing bromophenol blue alone was also introduced (loading buffer Edge). (top-bio.com)
- The two Axygen DNA Markers for Gel Electrophoresis require no additional preparation and contain bromphenol blue for ease of use. (selectscience.net)
- At low pH, the dye absorbs ultraviolet and blue light most strongly and appears yellow in solution. (wikipedia.org)
- 40 mg/ L ( 0 to 800 mg/ L range) Method heteropoly blue Number of Tests 100 CTK Type Checker Disc Ordering Information HI38067 test kit comes with 25 mL silica reage. (indonetwork.co.id)