Regulating the Golgi apparatus by co-delivery of a COX-2 inhibitor and Brefeldin A for suppression of tumor metastasis. (bvsalud.org)
In this study, treatment of EMT6 mouse mammary tumor cells with hypoxia or the chemical stress agents brefeldin A (BFA) or okadaic acid (OA) causes the development of resistance to the topoisomerase II inhibitor etoposide. (aspetjournals.org)
Brefeldin A was initially isolated with hopes to become an antiviral drug but is now primarily used in research to study protein transport. (wikipedia.org)
pAMPK)] and inhibition [dopamine-beta-hydroxylase GLUT3 MCT2] of protein expression in these cells responses Brefeldin A that were normalized Brefeldin A by insulin plus lactate treatment. (bioskinrevive.com)
However, if they are trapped inside the cell, they can be stained as intracellular proteins using protein transport inhibitors such as BD GolgiStop™ (containing monensin) or BD GolgiPlug™ (containing brefeldin A). Cytokines are relatively accessible using the gentle fixation and permeabilization afforded by BD Cytofix/ Cytoperm™ Fixation and Permeabilization Solution. (bdbiosciences.com)
Here we show that the AN gene encodes a C-terminal binding proteins/brefeldin A ADP-ribosylated substrates (CtBP/BARS) related protein. (nih.gov)
Salomons, F.A., I.J. van der Klei, A.M. Kram, W. Harder & M. Veenhuis (1997) Brefeldin A interferes with peroxisomal protein sorting in the yeast Hansenula polymorpha. (rug.nl)
Inhibits2
Brefeldin A reversibly inhibits the function of GBF1 uncompetitively by binding to the complex it forms with GDP-bound Arf1p and preventing conversion to the GTP-bound form. (wikipedia.org)
Brefeldin A blocks binding of ADP-ribosylation factor to the Golgi apparatus and inhibits GDP-GTP exchange. (adooq.com)
Secretion2
An important goal of the present work was to reconcile the role of GnRH nerve cell AMPK in the context Brefeldin A of metabolic restraint of pituitary LH secretion. (bioskinrevive.com)
Cilium trafficking of P/rds was sustained even when the Golgi functions, including trans -Golgi-mediated conventional secretion, were inhibited by the small molecules brefeldin A, 30N12, and monensin. (jneurosci.org)
Inhibition1
2007 a response that is abolished by AMPK inhibition [Roland and Brefeldin A Moenter 2011 Also immortalized mouse hypothalamic GT1-7 cells express AMPK and exhibit diminished GnRH release upon treatment with AMPK activators [Coyral-Castel et al. (bioskinrevive.com)
Proteins2
Brefeldin A (BFA) causes rapid redistribution of Golgi proteins into the endoplasmic reticulum (ER), leaving no definable Golgi-apparatus, and blocks transport of proteins from the ER to distal secretory compartments of the cell. (mpg.de)
We conclude that this 21 kDa and possibly also other GTP-binding proteins may be the molecular target of Brefeldin A in pancreatic acinar cells. (mpg.de)
Further, the uptake of NBD-PS-SWCNTs was suppressed by a cocktail of endocytosis inhibitors (chlorpromazine, nystatin, genistein and brefeldin A). This suggests that endocytosis is involved in the uptake of SWCNTs by macrophages. (cdc.gov)
Fungus1
Brefeldin A is a lactone antiviral produced by the fungus Penicillium brefeldianum. (wikipedia.org)
Membrane1
Disruption of carrier insertion with brefeldin A (5 µM) was followed by a decline of I( SMIT ) to a similar extent in Xenopus oocytes expressing SMIT with JAK2 and in Xenopus oocytes expressing SMIT alone, suggesting that JAK2 did not affect carrier stability in the cell membrane . (xenbase.org)
Cocktail1
PBMC stimulated with 1 μg/mL of aCD3 and 3 ug/ml of aCD28 for 2 days were treated with Tocris cell activation cocktail 500x (Catalog # 5476 ) and Brefeldin A (Catalog # 1231/5 ) for 3 hours or naïve PBMC lymphocytes. (rndsystems.com)
Yeast1
citation needed] In mammalian and yeast cells, the main target of brefeldin A appears to be a guanine nucleotide exchange factor (GEF) called GBF1. (wikipedia.org)
Cells2
Immunoprecipitation of ApoE from Hep G2 cells treated with Brefeldin A #9972 (10 ng/ml, 90 min), using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or ApoE (pan) (D7I9N) Rabbit mAb (lane 3). (cellsignal.com)
PMA+ionomycin-stimulated (6 hours, in presence of brefeldin A) Th2-polarized C57BL/6 CD4-positive cells were surface stained with CD3 PE and then intracellularly stained with IL-4 (11B11) APC (top) or rat IgG1, κ APC isotype control (bottom). (biolegend.com)
Assembly1
10.1021/acs.orglett.6b02002 ) a complementary assembly of brefeldin A ( 3 ), starting from glucose. (organic-chemistry.org)
Response1
the former Brefeldin A but not the latter response was reversed by lactate. (bioskinrevive.com)