• gels
  • Make sure the gels boxes are very clean (any mineral oil will ruin the blot, and we have boxes set aside strictly for Southerns). (ebioworld.com)
  • probes
  • To address our own needs to regularly design Southern blot probes, automating this process, reducing the time needed to do this manually. (genes2cognition.org)
  • DNA microarrays operate by similar procedures to those used in the reverse northern blot, consisting of many DNA probes hybridized to a solid glass, plastic or silicon substrate which is probed with labeled RNA or cDNA. (wikipedia.org)
  • cDNA
  • Size markers are indicated at the left side of C. The blot was hybridized with the entire insert from the full-length cDNA clone for RMCP-8 and the blot was washed under medium stringency conditions (2 × SSC and 0.5% SDS at 65°C). (nih.gov)
  • sequence
  • If we're talking eukaryotic cells, then the answer might simply be that the RNA in the Northern blot was mRNA and had introns excised, therefore making it different in sequence than the DNA from whence it was transcribed. (biology-online.org)
  • samples
  • one should be a 0.6% gel for the Southern, which can use two combs for samples that are run halfway or a single comb for samples that can be run further (half way is fine to get a decent, publishable result on the Cambridge boxes). (ebioworld.com)
  • Bulked DNA samples can be analysed using Southern blotting. (wikipedia.org)
  • Methods
  • The names for other blotting methods may follow this convention, by analogy. (wikipedia.org)
  • These large regions were termed "ultra-long" telomeres in the literature when they were identified using southern blotting and "mega-telomeres" when identified by cytogenetic methods. (wikipedia.org)
  • similar
  • As a result, zoo blotting is used to detect similar or exact relationships between the DNA in question and other organisms. (wikipedia.org)
  • techniques
  • Although DNA Microarrays and newer next-generation techniques have generally supplanted reverse northern blotting, it is still utilized today and provides a relatively cheap and easy means of defining expression of large sets of genes. (wikipedia.org)