- In biochemistry, biotinylation is the process of covalently attaching biotin to a protein, nucleic acid or other molecule. (wikipedia.org)
- Biotinylation is rapid, specific and is unlikely to disturb the natural function of the molecule due to the small size of biotin (MW = 244.31 g/mol). (wikipedia.org)
- Enzymatic biotinylation results in biotinylation of a specific lysine within a certain sequence by a bacterial biotin ligase. (wikipedia.org)
- Most chemical biotinylation reagents consist of a reactive group attached via a linker to the valeric acid side chain of biotin. (wikipedia.org)
- As the biotin binding pocket in avidin / streptavidin is buried beneath the protein surface, biotinylation reagents possessing a longer linker are desirable, as they enable the biotin molecule, once it has been attached to its target, to be more accessible to binding avidin/streptavidin/Neutravidin protein. (wikipedia.org)
- In contrast to chemical biotinylation methods, enzymatic biotinylation allows biotin to be linked at exactly one residue present in the protein. (wikipedia.org)
- Enzymatic biotinylation is most often carried out by E. coli biotin holoenzyme synthetase, also known as biotin ligase (BirA, P06709). (wikipedia.org)
- Once tagged, the protein is then incubated with BirA allowing biotinylation to take place in the presence of biotin and ATP. (wikipedia.org)
- Biotinylation of bls by each BirA was examined using anti-biotin antibody immunoblotting. (elifesciences.org)
- Biotinylations were performed at 500 nM of biotin at 26 °C for 1 hr (TurboID), 3 hr (AVVA-RG, AVVA-RS, and GFVA-RS), or 16 hr (BioID). (elifesciences.org)
- NHS Biotin is the simplest NHS ester activated biotinylation reagent for labeling primary amines such as protein lysines, The short spacer arm of NHS biotin allows membrane permeability. (soltecventures.com)
- Enzymatic biotinylation can be carried out in vitro but BirA also reacts specifically with its target peptide inside mammalian and bacterial cells and at the cell surface, while other cellular proteins are not modified. (wikipedia.org)
- Enzymatic biotinylation can also take place in vivo typically through the co-expression of an Avitag tagged protein and BirA. (wikipedia.org)
- Rat CD4 d3+4, enzymatic biotinylation sequence, linker and His tag (CD4d3+4-BioLHis). (addgene.org)
- NHS-coupling gives biotinylation of any primary amines in the protein). (wikipedia.org)
- Biotinylation by antibody recognition- A novel method for proximity labeling. (sspnet.org)
- There is a large number of biotinylation reagents available that exploit the wide range of possible labelling methods. (wikipedia.org)
- linkers that incorporate poly(ethylene) glycol (PEG) can make water-insoluble reagents soluble or increase the solubility of biotinylation reagents that are already soluble to some extent. (wikipedia.org)
- Amine-reactive biotinylation reagents can be divided into two groups based on water solubility. (wikipedia.org)
- Because of the hydrophobicity of NHS-esters, NHS biotinylation reagents can also diffuse through the cell membrane, meaning that they will biotinylate both internal and external components of a cell. (wikipedia.org)
- Here, we set up and evaluated the 'surface-spanning protein enrichment with click sugars' (SUSPECS) method for cell surface membrane glycoprotein biotinylation, enrichment and label-free quantitative mass spectrometry. (nih.gov)
- We present here an in vitro method that combines biotinylation and mass spectrometry (MS) to identify substrates deacetylated by sirtuins. (nyu.edu)
- Chemical biotinylation utilises various conjugation chemistries to yield nonspecific biotinylation of amines, carboxylates, sulfhydryls and carbohydrates (e.g. (wikipedia.org)
- Addgene: Split-BioID: a proximity-biotinylation assay for dimerization-dependent protein interactions. (addgene.org)
- Decreased histone biotinylation decreases life span and stress resistance in Drosophila. (nebraska.edu)
- C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag. (addgene.org)
- Other sources of experimental information include X-ray diffraction, biotinylation, sequence mutation and deletion, solution binding and chemical cross-linking data. (lu.se)
- Biotinylation" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (jefferson.edu)
- A ) RS mutants of AVVA and GFVA were cloned, and biotinylations of FLAG-GST-MDM2 (FG-MDM2) by BirA-p53 including RS mutants were analyzed. (elifesciences.org)
- B ) FG-RelA biotinylation by BirA-IκBα was examined. (elifesciences.org)
- in 2006, reported a simple and fast protocol for the biotinylation of carbohydrates (Figure 3). (biosyn.com)
- This biotinylation reaction can also go to completion, meaning that the product is generated with high uniformity and can be linked to streptavidin in a defined orientation e.g. for MHC multimers. (wikipedia.org)
- We also show that genetic silencing of BPL eliminated the pathogen efficiently from mice during acute and chronic infection with Mtb Partial chemical inactivation of BPL increased the potency of two first-line drugs, rifampicin and ethambutol, and genetic interference with protein biotinylation accelerated clearance of Mtb from mouse lungs and spleens by rifampicin. (tamu.edu)
- NHS-coupling gives biotinylation of any primary amines in the protein). (wikipedia.org)
- Enzymatic biotinylation can also take place in vivo typically through the co-expression of an Avitag tagged protein and BirA. (wikipedia.org)
- Protein biotinylation is a key post-translational modification found throughout the living world. (nih.gov)
- Phospholipid biotinylation of polydimethylsiloxane (PDMS) for protein immobilization. (bvsalud.org)
- BioID screening of biotinylation sites using the avidin-like protein Tamavidin 2-REV identifies global interactors of stimulator of interferon genes (STING). (nih.gov)
- The aim is tested in rodent models (rat, KCNMB1 K/0 mouse) using both in vitro (Western-blotting, protein biotinylation, ICC/IF microscopy, patch-clamp, cDNA reverse-permeabilization, and isolated myocytes and arteries) and in vivo methods (closed cranial window, intravital microscopy). (nih.gov)
- In order to gain a more detailed description of these events at the molecular level, we have developed a cell surface biotinylation assay to isolate, detect, and quantify neuronal membrane proteins. (unitn.it)
- Biotinylation is typically used to label proteins or nucleic acid based targets for these applications. (sigmaaldrich.com)
- Within the nucleus, biotinylation of DNA-associated proteins called histones may help determine whether certain genes are turned on or off. (medlineplus.gov)
- Surface membrane proteins of viable, intact cells were subjected to biotinylation then affinity-captured and purified on monomeric avidin columns. (nih.gov)
- Description: Biotinylation reagent with N-hydroxysuccinimide ester, and a 3 PEG spacer. (iowaodes.com)
- Kinase-Catalyzed Biotinylation to Map Cell Signaling Pathways: Application to Epidermal Growth Factor Signaling. (nih.gov)
- Quantitation of biotinylation can be used to optimize the labelling of a given target. (sigmaaldrich.com)
- Clone once with SNAP-tag® or CLIP-tag™ for multiple color fluorescent labeling, biotinylation or bead coupling of your target. (neb.com)
- Pukang dPEG biotinylation have characteristic design & practical performance & competitive price, for more information on the dPEG biotinylation , please feel free to contact us. (pu-kang.com)